Abstract:
:Bacterial nitric oxide (NO) synthases (bNOS) play diverse and important roles in microbial physiology, stress resistance, and virulence. Although bacterial and mammalian NOS enzymes have been well-characterized, comparatively little is known about the prevalence and function of NOS enzymes in Archaea. Analysis of archaeal genomes revealed that highly conserved bNOS homologs were restricted to members of the Halobacteria. Of these, Natronomonas pharaonis NOS (npNOS) was chosen for further characterization. NO production was confirmed in heterologously expressed His-tagged npNOS by coupling nitrite production from N-hydroxy-L-arginine in an H2O2-supported reaction. Additionally, the nos gene was successfully targeted and disrupted to create a Nmn. pharaonis nos mutant by adapting an established Natrialba magadii transformation protocol. Genome re-sequencing of this mutant revealed an additional frameshift in a putative cation-acetate symporter gene, which could contribute to altered acetate metabolism in the nos mutant. Inactivation of Nmn. pharaonis nos was also associated with several phenotypes congruent with bacterial nos mutants (altered growth, increased oxygen consumption, increased pigment, increased UV susceptibility), suggesting that NOS function may be conserved between bacteria and archaea. These studies are the first to describe genetic inactivation and characterization of a Nmn. pharaonis gene and provides enhanced tools for probing its physiology.
journal_name
Microbiologyopenjournal_title
MicrobiologyOpenauthors
Orsini SS,James KL,Reyes DJ,Couto-Rodriguez RL,Gulko MK,Witte A,Carroll RK,Rice KCdoi
10.1002/mbo3.1124subject
Has Abstractpub_date
2020-11-01 00:00:00pages
e1124issue
11issn
2045-8827journal_volume
9pub_type
杂志文章相关文献
MicrobiologyOpen文献大全abstract::The emerging pollutants antibiotic resistance genes (ARGs) are prevalent in aquatic environments such as estuary. Coastal mangrove ecosystems always serve as natural wetlands for receiving sewage which always carry ARGs. Currently, the research considering ARG distribution in mangrove ecosystems gains more interest. I...
journal_title:MicrobiologyOpen
pub_type: 杂志文章
doi:10.1002/mbo3.871
更新日期:2019-11-01 00:00:00
abstract::Xylella fastidiosa Temecula1 is the causative agent of Pierce's disease of grapevine, which is spread by xylem-feeding insects. An important feature of the infection cycle is the ability of X. fastidiosa to colonize and interact with two distinct environments, the xylem of susceptible plants and the insect foregut. He...
journal_title:MicrobiologyOpen
pub_type: 杂志文章
doi:10.1002/mbo3.6
更新日期:2012-03-01 00:00:00
abstract::The black pigmentation-related genes in Porphyromonas gingivalis are primarily involved in regulating gingipain functions. In this study, we identified a pigmentation-related gene, designated as pgn_0361. To characterize the role of pgn_0361 in regulating P. gingivalis-mediated epithelial cell detachment and inhibitio...
journal_title:MicrobiologyOpen
pub_type: 杂志文章
doi:10.1002/mbo3.1128
更新日期:2020-12-01 00:00:00
abstract::Eggerthella timonensis strain Marseille-P3135 is a new bacterial species, isolated from the stool sample of a healthy 8-year-old pygmy female. This strain (LT598568) showed a 16S rRNA sequence similarity of 96.95% with its phylogenetically closest species with standing in nomenclature Eggerthella lenta strain DSM 2243...
journal_title:MicrobiologyOpen
pub_type: 杂志文章
doi:10.1002/mbo3.575
更新日期:2018-10-01 00:00:00
abstract::Sporulation in Bacillus subtilis is induced upon starvation. In a widely accepted model, an N-terminal "sensor" domain of the major sporulation kinase KinA recognizes a hypothetical starvation signal(s) and autophosphorylates a histidine residue to activate the master regulator Spo0A via a multicomponent phosphorelay....
journal_title:MicrobiologyOpen
pub_type: 杂志文章
doi:10.1002/mbo3.481
更新日期:2017-08-01 00:00:00
abstract::Interactions between corals and their associated microbial communities (Symbiodiniaceae and prokaryotes) are key to understanding corals' potential for and rate of acclimatory and adaptive responses. However, the establishment of microalgal and bacterial communities is poorly understood during coral ontogeny in the wi...
journal_title:MicrobiologyOpen
pub_type: 杂志文章
doi:10.1002/mbo3.959
更新日期:2020-02-01 00:00:00
abstract::Microbially mediated decomposition of particulate organic carbon (POC) is a central component of the oceanic carbon cycle, controlling the flux of organic carbon from the surface ocean to the deep ocean. Yet, the specific microbial taxa responsible for POC decomposition and degradation in the deep ocean are still unkn...
journal_title:MicrobiologyOpen
pub_type: 杂志文章
doi:10.1002/mbo3.1013
更新日期:2020-05-01 00:00:00
abstract::Metal- and pesticide-tolerant biocontrol agents are preferred in integrated pest management, as such strains can be applied in combination with different pesticides. The Bacillus velezensis strain SZMC 6161J proved to be sensitive to copper, nickel, zinc, and cadmium, while manganese elevated its growth. At concentrat...
journal_title:MicrobiologyOpen
pub_type: 杂志文章
doi:10.1002/mbo3.660
更新日期:2019-03-01 00:00:00
abstract::The aim of this study was to determine the serogroups, antimicrobial resistance and genetic diversity of Escherichia coli isolates from samples of bivalve mollusks collected along Santa Catarina coast, Brazil, and from the Chesapeake Bay, Maryland, USA. One hundred forty-one E. coli isolates were characterized for ser...
journal_title:MicrobiologyOpen
pub_type: 杂志文章
doi:10.1002/mbo3.738
更新日期:2019-05-01 00:00:00
abstract::The Siamese crocodile (Crocodylus siamensis) is a freshwater, endangered crocodile with high economic value in the farming industry. Gut microflora plays an essential role in host physiological activity, and it contributes significantly to both the health and diseased states of animals. However, thus far, no study has...
journal_title:MicrobiologyOpen
pub_type: 杂志文章
doi:10.1002/mbo3.929
更新日期:2019-12-01 00:00:00
abstract::Gene expression in Haloarcula hispanica cells infected with the gammapleolipovirus His2 was studied using a custom DNA microarray. Total RNA from cells sampled at 0, 1, 2, 3, and 4.5 hr postinfection was reverse-transcribed into labeled cDNA and hybridized to microarrays, revealing temporal and differential expression...
journal_title:MicrobiologyOpen
pub_type: 杂志文章
doi:10.1002/mbo3.1016
更新日期:2020-05-01 00:00:00
abstract::Human gut microbiome dysbiosis has been associated with the onset of metabolic diseases and disorders. However, the critical factors leading to dysbiosis are poorly understood. In this study, we provide increasing evidence of the association of diet type and body mass index (BMI) and how they relatively influence the ...
journal_title:MicrobiologyOpen
pub_type: 杂志文章
doi:10.1002/mbo3.476
更新日期:2017-08-01 00:00:00
abstract::Genomic analysis points to N-glycosylation as being a common posttranslational modification in Archaea. To date, however, pathways of archaeal N-glycosylation have only been described for few species. With this in mind, the similarities of N-linked glycans decorating glycoproteins in the haloarchaea Haloferax volcanii...
journal_title:MicrobiologyOpen
pub_type: 杂志文章
doi:10.1002/mbo3.215
更新日期:2015-02-01 00:00:00
abstract::Nuclease colicins bind their target receptor BtuB in the outer membrane of sensitive Escherichia coli cells in the form of a high-affinity complex with their cognate immunity proteins. The release of the immunity protein from the colicin complex is a prerequisite for cell entry of the colicin and occurs via a process ...
journal_title:MicrobiologyOpen
pub_type: 杂志文章
doi:10.1002/mbo3.122
更新日期:2013-10-01 00:00:00
abstract::The Hatton-Rockall Basin (North-East Atlantic) is an area with potential for deep-sea (2,900 m) hydrocarbon exploration. Following the Deepwater Horizon oil spill, many investigations into the responses of sediment microbial communities to oil pollution have been undertaken. However, hydrostatic pressure is a paramete...
journal_title:MicrobiologyOpen
pub_type: 杂志文章
doi:10.1002/mbo3.768
更新日期:2019-06-01 00:00:00
abstract::Extracts from white birch have been reported to possess antimicrobial properties, but no study has linked the chemical composition of bark extract with antimicrobial activity. This study aimed to identify white birch (Betula papyrifera Marshall) bark extracts with antimicrobial activity and elucidate its composition. ...
journal_title:MicrobiologyOpen
pub_type: 杂志文章
doi:10.1002/mbo3.944
更新日期:2020-01-01 00:00:00
abstract::Despite the relevance of landscape, regarding the spatial patterning of microbial communities and the relative influence of environmental parameters versus human activities, few investigations have been conducted at this scale. Here, we used a systematic grid to characterize the distribution of soil microbial communit...
journal_title:MicrobiologyOpen
pub_type: 杂志文章
doi:10.1002/mbo3.255
更新日期:2015-06-01 00:00:00
abstract::Dinitrogen fixation, the biological reduction in N2 gas to ammonia contributes to the supply of new nitrogen in the surface ocean. To understand the diversity and abundance of potentially diazotrophic (N2 fixing) microorganisms associated with marine zooplankton, especially copepods, the nifH gene was studied using zo...
journal_title:MicrobiologyOpen
pub_type: 杂志文章
doi:10.1002/mbo3.385
更新日期:2016-12-01 00:00:00
abstract::UV resistance of bacteria isolated from the marine cyanobacterium Moorea producens has not been observed previously, findings which highlight how unsafe germicidal UV irradiation for sterilization of air, food, and water could be. Further, UV resistance of Bacillus licheniformis is being observed for the first time. T...
journal_title:MicrobiologyOpen
pub_type: 杂志文章
doi:10.1002/mbo3.697
更新日期:2019-04-01 00:00:00
abstract::A yeast expression plasmid was constructed containing a cardenolide biosynthetic module, referred to as CARD II, using the AssemblX toolkit, which enables the assembly of large DNA constructs. The genes cloned into the vector were (a) a Δ5 -3β-hydroxysteroid dehydrogenase gene from Digitalis lanata, (b) a steroid Δ5 -...
journal_title:MicrobiologyOpen
pub_type: 杂志文章
doi:10.1002/mbo3.925
更新日期:2019-12-01 00:00:00
abstract::Newcastle disease, caused by virulent strain of Newcastle disease virus (NDV), is an acute, highly contagious disease that is prevalent worldwide and is responsible for serious economic losses to the poultry industry. In the current study, we compared the early immune responses in chickens infected with two strains of...
journal_title:MicrobiologyOpen
pub_type: 杂志文章
doi:10.1002/mbo3.701
更新日期:2019-04-01 00:00:00
abstract::In cyanobacteria, genes conferring mercury resistance are not organized as mer-operon, unlike in other bacterial phyla. Synechocystis contains only a putative MerR regulator, Slr0701, and a mercury reductase, MerA, located aside from each other in the genome. The slr0701-mutant showed reduction in photosynthetic activ...
journal_title:MicrobiologyOpen
pub_type: 杂志文章
doi:10.1002/mbo3.838
更新日期:2019-09-01 00:00:00
abstract::Kalimantacin is an antimicrobial compound with strong antistaphylococcal activity that is produced by a hybrid trans-acyltransferase polyketide synthase/nonribosomal peptide synthetase system in Pseudomonas fluorescens BCCM_ID9359. We here present a systematic analysis of the substrate specificity of the glycine-incor...
journal_title:MicrobiologyOpen
pub_type: 杂志文章
doi:10.1002/mbo3.326
更新日期:2016-04-01 00:00:00
abstract::Trying to widen the discussion on the risks associated with dental waste, this study proposed to investigate and genetically compare yeast isolates recovered from dental solid waste and waste workers. Three samples were collected from workers' hands, nasal mucosa, and professional clothing (days 0, 30, and 180), and t...
journal_title:MicrobiologyOpen
pub_type: 杂志文章
doi:10.1002/mbo3.554
更新日期:2018-04-01 00:00:00
abstract::Sustained monoculture often leads to the inhibition of plant growth, the decrease of the soil microbial diversity, and changes in soil microbial community composition, particularly to the accumulation of soil-borne pathogens. In this study, we conducted field experiments to investigate the practical effects of tilling...
journal_title:MicrobiologyOpen
pub_type: 杂志文章
doi:10.1002/mbo3.1045
更新日期:2020-07-01 00:00:00
abstract::Aflatoxin M1 (AFM1 ) is a potent mycotoxin which causes serious health concerns in developing countries, where it is mainly found in milk, meat, and other foods. Biological detoxification is a promising method for eliminating AFM1 . The aim of this work was to search for AFM1 -degrading bacterial strains from animal w...
journal_title:MicrobiologyOpen
pub_type: 杂志文章
doi:10.1002/mbo3.663
更新日期:2019-03-01 00:00:00
abstract::Phellinus noxius is a pathogenic fungus that causes brown root rot disease, resulting in a widespread tree and crop mortality in the tropics and subtropics. Early stages of this disease are largely asymptomatic, hindering early diagnosis and effective treatment. We hypothesized that P. noxius infection would alter the...
journal_title:MicrobiologyOpen
pub_type: 杂志文章
doi:10.1002/mbo3.1115
更新日期:2020-10-01 00:00:00
abstract::Rhodothermus marinus, a marine aerobic thermophile, was first isolated from an intertidal hot spring in Iceland. In recent years, the R. marinus strain PRI 493 has been genetically modified, which opens up possibilities for targeted metabolic engineering of the species, such as of the carotenoid biosynthetic pathway. ...
journal_title:MicrobiologyOpen
pub_type: 杂志文章
doi:10.1002/mbo3.536
更新日期:2018-02-01 00:00:00
abstract::Probiotic bacteria are frequently used to treat intestinal diseases or to improve health; however, little is known about the evolutionary changes of these bacteria during probiotic manufacture and the bacterial ability to colonize the intestine. It has been observed that when bacteria adapt to a new environment, they ...
journal_title:MicrobiologyOpen
pub_type: 杂志文章
doi:10.1002/mbo3.972
更新日期:2020-02-01 00:00:00
abstract::The extreme radiation resistance of Deinococcus bacteria requires the radiation-stimulated cleavage of protein DdrO by a specific metalloprotease called IrrE. DdrO is the repressor of a predicted radiation/desiccation response (RDR) regulon, composed of radiation-induced genes having a conserved DNA motif (RDRM) in th...
journal_title:MicrobiologyOpen
pub_type: 杂志文章
doi:10.1002/mbo3.477
更新日期:2017-08-01 00:00:00