Abstract:
:Microbial biofilms are communities of surface-adhered cells enclosed in a matrix of extracellular polymeric substances. Bacterial cells in biofilm are 10~1,000-fold more resistant to antimicrobials than the planktonic cells. Burgeoning antibiotic resistance in Pseudomonas aeruginosa biofilm has necessitated the development of antimicrobial agents. Here, we have investigated the antibiofilm effect of meloxicam against P. aeruginosaPAO1 and its potential mechanisms. Further, we have explored whether meloxicam could enhance the susceptibility of bacterial biofilms to treatment with conventional antimicrobials. Here, we found that meloxicam could significantly inhibit PAO1 biofilm formation in a dose-dependent manner at the concentration without influence on planktonic cell growth. Meloxicam could also significantly inhibit the motilities, production of extracellular matrix, and expression of quorum sensing-related genes and virulence factors of PAO1. Furthermore, synergistic interaction was observed when meloxicam combined with tetracycline, gentamicin, tobramycin, ciprofloxacin, ceftriaxone, ofloxacin, norfloxacin, ceftazidime, and DNase at subminimal inhibitory concentrations against PAO1 bioiflm. Collectively, our study lays the foundation for further investigation of repurposing meloxicam as a topical antibiofilm agent to treat P. aeruginosa biofilm-related infections.
journal_name
Microbiologyopenjournal_title
MicrobiologyOpenauthors
She P,Wang Y,Luo Z,Chen L,Tan R,Wang Y,Wu Ydoi
10.1002/mbo3.545subject
Has Abstractpub_date
2018-02-01 00:00:00issue
1issn
2045-8827journal_volume
7pub_type
杂志文章相关文献
MicrobiologyOpen文献大全abstract::RodZ is important for maintaining the rod shape of Escherichia coli. Loss of RodZ causes conversion of the rod shape to a round shape and a growth rate slower than that of wild-type cells. Suppressor mutations that simultaneously restore both the growth rates and the rod shape were isolated. Most of the suppressor mut...
journal_title:MicrobiologyOpen
pub_type: 杂志文章
doi:10.1002/mbo3.116
更新日期:2013-10-01 00:00:00
abstract::Protein lysine acetylation, a dynamic and reversible posttranslational modification, plays a crucial role in several cellular processes, including cell cycle regulation, metabolism, enzymatic activities, and protein interactions. Brenneria nigrifluens is a pathogen of walnut trees with shallow bark canker and can caus...
journal_title:MicrobiologyOpen
pub_type: 杂志文章
doi:10.1002/mbo3.952
更新日期:2020-01-01 00:00:00
abstract::Citrus canker is an economically important disease that affects orange production in some of the most important producing areas around the world. It represents a great threat to the Brazilian and North American citriculture, particularly to the states of São Paulo and Florida, which together correspond to the biggest ...
journal_title:MicrobiologyOpen
pub_type: 杂志文章
doi:10.1002/mbo3.323
更新日期:2016-04-01 00:00:00
abstract::Metal- and pesticide-tolerant biocontrol agents are preferred in integrated pest management, as such strains can be applied in combination with different pesticides. The Bacillus velezensis strain SZMC 6161J proved to be sensitive to copper, nickel, zinc, and cadmium, while manganese elevated its growth. At concentrat...
journal_title:MicrobiologyOpen
pub_type: 杂志文章
doi:10.1002/mbo3.660
更新日期:2019-03-01 00:00:00
abstract::Copper resistance system in the cyanobacterium Synechocystis sp. PCC 6803 comprises two operons, copMRS and copBAC, which are expressed in response to copper in the media. copBAC codes for a heavy-metal efflux-resistance nodulation and division (HME-RND) system, while copMRS codes for a protein of unknown function, Co...
journal_title:MicrobiologyOpen
pub_type: 杂志文章
doi:10.1002/mbo3.231
更新日期:2015-02-01 00:00:00
abstract::The black pigmentation-related genes in Porphyromonas gingivalis are primarily involved in regulating gingipain functions. In this study, we identified a pigmentation-related gene, designated as pgn_0361. To characterize the role of pgn_0361 in regulating P. gingivalis-mediated epithelial cell detachment and inhibitio...
journal_title:MicrobiologyOpen
pub_type: 杂志文章
doi:10.1002/mbo3.1128
更新日期:2020-12-01 00:00:00
abstract::The small hive beetle (SHB) is an opportunistic parasite that feeds on bee larvae, honey, and pollen. While SHBs can also feed on fruit and other plant products, like its plant-feeding relatives, SHBs prefer to feed on hive resources and only reproduce inside bee colonies. As parasites, SHBs are inevitably exposed to ...
journal_title:MicrobiologyOpen
pub_type: 杂志文章
doi:10.1002/mbo3.899
更新日期:2019-10-01 00:00:00
abstract::Pseudomonas aeruginosa is a good environmental microorganism capable of degrading decabromodiphenyl ether (BDE-209). This paper studied the effect of Cu2+ and humic acid (HA) extracted from e-waste contaminated soils on biodegradation of BDE-209 by P. aeruginosa. The adsorption isotherms of Cu2+ on HA, the crude enzym...
journal_title:MicrobiologyOpen
pub_type: 杂志文章
doi:10.1002/mbo3.439
更新日期:2017-06-01 00:00:00
abstract::Streptococcus dysgalactiae subsp. dysgalactiae (SDSD) is a major cause of bovine mastitis and has been regarded as an animal-restricted pathogen, although rare infections have been described in humans. Previous studies revealed the presence of virulence genes encoded by phages of the human pathogen Group A Streptococc...
journal_title:MicrobiologyOpen
pub_type: 杂志文章
doi:10.1002/mbo3.623
更新日期:2019-01-01 00:00:00
abstract::Bacterial nitric oxide (NO) synthases (bNOS) play diverse and important roles in microbial physiology, stress resistance, and virulence. Although bacterial and mammalian NOS enzymes have been well-characterized, comparatively little is known about the prevalence and function of NOS enzymes in Archaea. Analysis of arch...
journal_title:MicrobiologyOpen
pub_type: 杂志文章
doi:10.1002/mbo3.1124
更新日期:2020-11-01 00:00:00
abstract::The emerging pollutants antibiotic resistance genes (ARGs) are prevalent in aquatic environments such as estuary. Coastal mangrove ecosystems always serve as natural wetlands for receiving sewage which always carry ARGs. Currently, the research considering ARG distribution in mangrove ecosystems gains more interest. I...
journal_title:MicrobiologyOpen
pub_type: 杂志文章
doi:10.1002/mbo3.871
更新日期:2019-11-01 00:00:00
abstract::Marine picocyanobacteria belonging to genera Synechococcus and Prochlorococcus are genetically diverged and distributed into distinct biogeographical patterns, and both are infected by genetically closely related cyanopodoviruses. Previous studies have not fully explored whether the two virus-host systems share simila...
journal_title:MicrobiologyOpen
pub_type: 杂志文章
doi:10.1002/mbo3.1150
更新日期:2020-12-30 00:00:00
abstract::Betaproteobacteria were the most common isolates from the water-filled tank of a Costa Rican bromeliad. Isolates included eight species from the orders Neisseriales and Burkholderiales, with close relatives recovered previously from tropical soils, wetlands, freshwater, or in association with plants. Compared to close...
journal_title:MicrobiologyOpen
pub_type: 杂志文章
doi:10.1002/mbo3.344
更新日期:2016-06-01 00:00:00
abstract::Nuclease colicins bind their target receptor BtuB in the outer membrane of sensitive Escherichia coli cells in the form of a high-affinity complex with their cognate immunity proteins. The release of the immunity protein from the colicin complex is a prerequisite for cell entry of the colicin and occurs via a process ...
journal_title:MicrobiologyOpen
pub_type: 杂志文章
doi:10.1002/mbo3.122
更新日期:2013-10-01 00:00:00
abstract::During mixed-acid fermentation Escherichia coli produces formate, which is initially excreted out the cell. Accumulation of formate, and dropping extracellular pH, leads to biosynthesis of the formate hydrogenlyase (FHL) complex. FHL consists of membrane and soluble domains anchored within the inner membrane. The solu...
journal_title:MicrobiologyOpen
pub_type: 杂志文章
doi:10.1002/mbo3.365
更新日期:2016-10-01 00:00:00
abstract::Pseudomonas aeruginosa and Staphylococcus aureus mixed-species biofilm infections are more resilient to biocide attacks compared to their single-species counterparts. Therefore, this study used an in vitro model recapitulating bacterial burdens seen in in vivo infections to investigate the interactions of P. aeruginos...
journal_title:MicrobiologyOpen
pub_type: 杂志文章
doi:10.1002/mbo3.427
更新日期:2017-06-01 00:00:00
abstract::In cyanobacteria, genes conferring mercury resistance are not organized as mer-operon, unlike in other bacterial phyla. Synechocystis contains only a putative MerR regulator, Slr0701, and a mercury reductase, MerA, located aside from each other in the genome. The slr0701-mutant showed reduction in photosynthetic activ...
journal_title:MicrobiologyOpen
pub_type: 杂志文章
doi:10.1002/mbo3.838
更新日期:2019-09-01 00:00:00
abstract::The intraspecies genomic diversity of the single-strand RNA (+) virus species hepatitis A virus (Hepatovirus), hepatitis C virus (Hepacivirus), and hepatitis E virus (Orthohepevirus) was compared. These viral species all can cause liver inflammation (hepatitis), but share no gene similarity. The codon usage of human h...
journal_title:MicrobiologyOpen
pub_type: 杂志文章
doi:10.1002/mbo3.973
更新日期:2020-02-01 00:00:00
abstract::Sucrose is an important disaccharide used as a substrate in many industrial applications. It is a major component of molasses, a cheap by-product of the sugar industry. Unfortunately, not all industrially relevant organisms, among them Pseudomonas putida, are capable of metabolizing sucrose. We chose a metabolic engin...
journal_title:MicrobiologyOpen
pub_type: 杂志文章
doi:10.1002/mbo3.473
更新日期:2017-08-01 00:00:00
abstract::FtsZ - a prokaryotic tubulin homolog - is one of the central components of bacterial division machinery. At the early stage of cytokinesis FtsZ forms the so-called Z-ring at mid-cell that guides septum formation. Many approaches were used to resolve the structure of the Z-ring, however, researchers are still far from ...
journal_title:MicrobiologyOpen
pub_type: 杂志文章
doi:10.1002/mbo3.336
更新日期:2016-06-01 00:00:00
abstract::In enterohemorrhagic Escherichia coli (EHEC), sigma factor N (σ(N)) regulates glutamate-dependent acid resistance (GDAR) and the locus of enterocyte effacement (LEE); discrete genetic systems that are required for transmission and virulence of this intestinal pathogen. Regulation of these systems requires nitrogen reg...
journal_title:MicrobiologyOpen
pub_type: 杂志文章
doi:10.1002/mbo3.183
更新日期:2014-08-01 00:00:00
abstract::The Hatton-Rockall Basin (North-East Atlantic) is an area with potential for deep-sea (2,900 m) hydrocarbon exploration. Following the Deepwater Horizon oil spill, many investigations into the responses of sediment microbial communities to oil pollution have been undertaken. However, hydrostatic pressure is a paramete...
journal_title:MicrobiologyOpen
pub_type: 杂志文章
doi:10.1002/mbo3.768
更新日期:2019-06-01 00:00:00
abstract::Methicillin-resistant S. aureus has been reported as the main pathogen involved in chronic infections, osteomyelitis, and prosthetic joint infections. The host/pathogen interaction is dynamic and requires several changes to promote bacterial survival. Here, we focused on the internalization and persistence behavior of...
journal_title:MicrobiologyOpen
pub_type: 杂志文章
doi:10.1002/mbo3.1017
更新日期:2020-05-01 00:00:00
abstract::Vibrio coralliilyticus is an important coral pathogen demonstrated to cause disease outbreaks worldwide. This study investigated the feasibility of applying bacteriophage therapy to treat the coral pathogen V. coralliilyticus. A specific bacteriophage for V. coralliilyticus strain P1 (LMG23696), referred to here as ba...
journal_title:MicrobiologyOpen
pub_type: 杂志文章
doi:10.1002/mbo3.52
更新日期:2013-02-01 00:00:00
abstract::Dinitrogen fixation, the biological reduction in N2 gas to ammonia contributes to the supply of new nitrogen in the surface ocean. To understand the diversity and abundance of potentially diazotrophic (N2 fixing) microorganisms associated with marine zooplankton, especially copepods, the nifH gene was studied using zo...
journal_title:MicrobiologyOpen
pub_type: 杂志文章
doi:10.1002/mbo3.385
更新日期:2016-12-01 00:00:00
abstract::Gene expression in Haloarcula hispanica cells infected with the gammapleolipovirus His2 was studied using a custom DNA microarray. Total RNA from cells sampled at 0, 1, 2, 3, and 4.5 hr postinfection was reverse-transcribed into labeled cDNA and hybridized to microarrays, revealing temporal and differential expression...
journal_title:MicrobiologyOpen
pub_type: 杂志文章
doi:10.1002/mbo3.1016
更新日期:2020-05-01 00:00:00
abstract::Microbially mediated decomposition of particulate organic carbon (POC) is a central component of the oceanic carbon cycle, controlling the flux of organic carbon from the surface ocean to the deep ocean. Yet, the specific microbial taxa responsible for POC decomposition and degradation in the deep ocean are still unkn...
journal_title:MicrobiologyOpen
pub_type: 杂志文章
doi:10.1002/mbo3.1013
更新日期:2020-05-01 00:00:00
abstract::The effect of elevated atmospheric CO2 concentration [CO2 ] on the diversity and composition of the prokaryotic community inhabiting the rhizosphere of winter barley (Hordeum vulgare L.) was investigated in a field experiment, using open-top chambers. Rhizosphere samples were collected at anthesis (flowering stage) fr...
journal_title:MicrobiologyOpen
pub_type: 杂志文章
doi:10.1002/mbo3.462
更新日期:2017-08-01 00:00:00
abstract::Sourdough fermentation is a traditional process that is used to improve bread quality. A spontaneous sourdough ecosystem consists of a mixture of flour and water that is fermented by endogenous lactic acid bacteria (LAB) and yeasts. The aim of this study was to identify bacterial diversity during backslopping of spont...
journal_title:MicrobiologyOpen
pub_type: 杂志文章
doi:10.1002/mbo3.1009
更新日期:2020-04-01 00:00:00
abstract::Bacterial community structures were evaluated in oil samples using culture-independent pyrosequencing, including oil mousses collected on sea surface and salt marshes during the Deepwater Horizon oil spill, and oil deposited in sediments adjacent to the wellhead 1 year after the spill. Phylogenetic analysis suggested ...
journal_title:MicrobiologyOpen
pub_type: 杂志文章
doi:10.1002/mbo3.89
更新日期:2013-06-01 00:00:00