Posttranslational modification of a vanadium nitrogenase.

Abstract:

:In microbes that fix nitrogen, nitrogenase catalyzes the conversion of N2 to ammonia in an ATP-demanding reaction. To help conserve energy some bacteria inhibit nitrogenase activity upon exposure to ammonium. The purple nonsulfur phototrophic bacterium Rhodopseudomonas palustris strain CGA009 can synthesize three functional nitrogenase isoenzymes: a molybdenum nitrogenase, a vanadium nitrogenase, and an iron nitrogenase. Previous studies showed that in some alphaproteobacteria, including R. palustris, molybdenum nitrogenase activity is inhibited by ADP-ribosylation when cells are exposed to ammonium. Some iron nitrogenases are also posttranslationally modified. However, the posttranslational modification of vanadium nitrogenase has not been reported. Here, we investigated the regulation of the alternative nitrogenases of R. palustris and determined that both its vanadium nitrogenase and its iron nitrogenase activities were inhibited and posttranslationally modified when cells are exposed to ammonium. Vanadium nitrogenase is not found in all strains of R. palustris, suggesting that it may have been acquired by horizontal gene transfer. Also, phylogenetic analyses of the three nitrogenases suggest that VnfH, the target of ADP-ribosylation, may be the product of a gene duplication of nifH, the molybdenum nitrogenase homolog.

journal_name

Microbiologyopen

journal_title

MicrobiologyOpen

authors

Heiniger EK,Harwood CS

doi

10.1002/mbo3.265

subject

Has Abstract

pub_date

2015-08-01 00:00:00

pages

597-603

issue

4

issn

2045-8827

journal_volume

4

pub_type

杂志文章
  • XatA, an AT-1 autotransporter important for the virulence of Xylella fastidiosa Temecula1.

    abstract::Xylella fastidiosa Temecula1 is the causative agent of Pierce's disease of grapevine, which is spread by xylem-feeding insects. An important feature of the infection cycle is the ability of X. fastidiosa to colonize and interact with two distinct environments, the xylem of susceptible plants and the insect foregut. He...

    journal_title:MicrobiologyOpen

    pub_type: 杂志文章

    doi:10.1002/mbo3.6

    authors: Matsumoto A,Huston SL,Killiny N,Igo MM

    更新日期:2012-03-01 00:00:00

  • Conservation and diversity of the IrrE/DdrO-controlled radiation response in radiation-resistant Deinococcus bacteria.

    abstract::The extreme radiation resistance of Deinococcus bacteria requires the radiation-stimulated cleavage of protein DdrO by a specific metalloprotease called IrrE. DdrO is the repressor of a predicted radiation/desiccation response (RDR) regulon, composed of radiation-induced genes having a conserved DNA motif (RDRM) in th...

    journal_title:MicrobiologyOpen

    pub_type: 杂志文章

    doi:10.1002/mbo3.477

    authors: Blanchard L,Guérin P,Roche D,Cruveiller S,Pignol D,Vallenet D,Armengaud J,de Groot A

    更新日期:2017-08-01 00:00:00

  • Thermoelectric heat exchange and growth regulation in a continuous yeast culture.

    abstract::We have designed a thermoelectric heat exchanger (TEHE) for microbial fermentations that is able to produce electric power from a microbial continuous culture using the intrinsic heat generated by microbial growth. While the TEHE was connected, the system proved able to stably self-maintain both the temperature and th...

    journal_title:MicrobiologyOpen

    pub_type: 杂志文章

    doi:10.1002/mbo3.648

    authors: Latorre-Pérez A,Vilanova C,Alcaina JJ,Porcar M

    更新日期:2019-03-01 00:00:00

  • Noncontiguous finished genome sequence and description of Raoultibacter massiliensis gen. nov., sp. nov. and Raoultibacter timonensis sp. nov, two new bacterial species isolated from the human gut.

    abstract::As part of the culturomics project aiming at describing the human microbiota, we report in this study the description of the new bacterial genus Raoultibacter gen. nov. that includes two new species, that is, R. massiliensis sp. nov. and R. timonensis sp. nov. The R. massiliensis type strain Marseille-P2849T was isola...

    journal_title:MicrobiologyOpen

    pub_type: 杂志文章

    doi:10.1002/mbo3.758

    authors: Traore SI,Bilen M,Beye M,Diop A,Mbogning Fonkou MD,Tall ML,Michelle C,Yasir M,Ibraheem Azhar E,Bibi F,Bittar F,Jiman-Fatani AA,Daoud Z,Cadoret F,Fournier PE,Edouard S

    更新日期:2019-06-01 00:00:00

  • A membrane-bound [NiFe]-hydrogenase large subunit precursor whose C-terminal extension is not essential for cofactor incorporation but guarantees optimal maturation.

    abstract::[NiFe]-hydrogenases catalyze the reversible conversion of molecular hydrogen into protons end electrons. This reaction takes place at a NiFe(CN)2 (CO) cofactor located in the large subunit of the bipartite hydrogenase module. The corresponding apo-protein carries usually a C-terminal extension that is cleaved off by a...

    journal_title:MicrobiologyOpen

    pub_type: 杂志文章

    doi:10.1002/mbo3.1029

    authors: Hartmann S,Frielingsdorf S,Caserta G,Lenz O

    更新日期:2020-06-01 00:00:00

  • Immunogenicity of a bivalent protein as a vaccine against Edwardsiella anguillarum and Vibrio vulnificus in Japanese eel (Anguilla japonica).

    abstract::The OMPs A (OmpA)-of Edwardsiella anguillarum and OmpU of V. vulnificus have been proven to be good antigens. In this study, after construction of a vector, a new recombinant Omp (rOMP) containing both OmpA and OmpU was expressed and purified. Then, the Japanese eels (Anguilla japonica) were intraperitoneally (i.p.) i...

    journal_title:MicrobiologyOpen

    pub_type: 杂志文章

    doi:10.1002/mbo3.766

    authors: Guo S,Hu L,Feng J,Lin P,He L,Yan Q

    更新日期:2019-06-01 00:00:00

  • DNA stable-isotope probing reveals potential key players for microbial decomposition and degradation of diatom-derived marine particulate matter.

    abstract::Microbially mediated decomposition of particulate organic carbon (POC) is a central component of the oceanic carbon cycle, controlling the flux of organic carbon from the surface ocean to the deep ocean. Yet, the specific microbial taxa responsible for POC decomposition and degradation in the deep ocean are still unkn...

    journal_title:MicrobiologyOpen

    pub_type: 杂志文章

    doi:10.1002/mbo3.1013

    authors: Liu Y,Fang J,Jia Z,Chen S,Zhang L,Gao W

    更新日期:2020-05-01 00:00:00

  • Glycolipid composition of the heterocyst envelope of Anabaena sp. PCC 7120 is crucial for diazotrophic growth and relies on the UDP-galactose 4-epimerase HgdA.

    abstract::The nitrogenase complex in the heterocysts of the filamentous freshwater cyanobacterium Anabaenasp. PCC 7120 fixes atmospheric nitrogen to allow diazotrophic growth. The heterocyst cell envelope protects the nitrogenase from oxygen and consists of a polysaccharide and a glycolipid layer that are formed by a complex pr...

    journal_title:MicrobiologyOpen

    pub_type: 杂志文章

    doi:10.1002/mbo3.811

    authors: Shvarev D,Nishi CN,Maldener I

    更新日期:2019-08-01 00:00:00

  • Microbial composition of enigmatic bird parasites: Wolbachia and Spiroplasma are the most important bacterial associates of quill mites (Acariformes: Syringophilidae).

    abstract:BACKGROUND:The microbiome is an integral component of many animal species, potentially affecting behavior, physiology, and other biological properties. Despite this importance, bacterial communities remain vastly understudied in many groups of invertebrates, including mites. Quill mites (Acariformes: Syringophilidae) a...

    journal_title:MicrobiologyOpen

    pub_type: 杂志文章

    doi:10.1002/mbo3.964

    authors: Glowska E,Filutowska ZK,Dabert M,Gerth M

    更新日期:2020-05-01 00:00:00

  • Mcr colistin resistance gene: a systematic review of current diagnostics and detection methods.

    abstract::Resistance to colistin, mediated by chromosomal mutations and more recently, by plasmid-borne mcr genes, is increasingly being reported in bacterial isolates taken from humans, animals, farms, foods, and the environment. To easily identify and contain this quickly spreading menace, efficient diagnostics that are cheap...

    journal_title:MicrobiologyOpen

    pub_type: 杂志文章

    doi:10.1002/mbo3.682

    authors: Osei Sekyere J

    更新日期:2019-04-01 00:00:00

  • Insights into gene regulation of the halovirus His2 infecting Haloarcula hispanica.

    abstract::Gene expression in Haloarcula hispanica cells infected with the gammapleolipovirus His2 was studied using a custom DNA microarray. Total RNA from cells sampled at 0, 1, 2, 3, and 4.5 hr postinfection was reverse-transcribed into labeled cDNA and hybridized to microarrays, revealing temporal and differential expression...

    journal_title:MicrobiologyOpen

    pub_type: 杂志文章

    doi:10.1002/mbo3.1016

    authors: Lee STM,Ding JY,Chiang PW,Dyall-Smith M,Tang SL

    更新日期:2020-05-01 00:00:00

  • Eggerthella timonensis sp. nov, a new species isolated from the stool sample of a pygmy female.

    abstract::Eggerthella timonensis strain Marseille-P3135 is a new bacterial species, isolated from the stool sample of a healthy 8-year-old pygmy female. This strain (LT598568) showed a 16S rRNA sequence similarity of 96.95% with its phylogenetically closest species with standing in nomenclature Eggerthella lenta strain DSM 2243...

    journal_title:MicrobiologyOpen

    pub_type: 杂志文章

    doi:10.1002/mbo3.575

    authors: Bilen M,Fonkou MDM,Tomei E,Armstrong N,Bittar F,Lagier JC,Daoud Z,Fournier PE,Raoult D,Cadoret F

    更新日期:2018-10-01 00:00:00

  • The DNABII family of proteins is comprised of the only nucleoid associated proteins required for nontypeable Haemophilus influenzae biofilm structure.

    abstract::Biofilms play a central role in the pathobiology of otitis media (OM), bronchitis, sinusitis, conjunctivitis, and pneumonia caused by nontypeable Haemophilus influenzae (NTHI). Our previous studies show that extracellular DNA (eDNA) and DNABII proteins are essential components of biofilms formed by NTHI. The DNABII pr...

    journal_title:MicrobiologyOpen

    pub_type: 杂志文章

    doi:10.1002/mbo3.563

    authors: Devaraj A,Buzzo J,Rocco CJ,Bakaletz LO,Goodman SD

    更新日期:2018-06-01 00:00:00

  • Global transcriptome responses including small RNAs during mixed-species interactions with methicillin-resistant Staphylococcus aureus and Pseudomonas aeruginosa.

    abstract::Pseudomonas aeruginosa and Staphylococcus aureus mixed-species biofilm infections are more resilient to biocide attacks compared to their single-species counterparts. Therefore, this study used an in vitro model recapitulating bacterial burdens seen in in vivo infections to investigate the interactions of P. aeruginos...

    journal_title:MicrobiologyOpen

    pub_type: 杂志文章

    doi:10.1002/mbo3.427

    authors: Miller CL,Van Laar TA,Chen T,Karna SLR,Chen P,You T,Leung KP

    更新日期:2017-06-01 00:00:00

  • The role of compatible solutes in desiccation resistance of Acinetobacter baumannii.

    abstract::Acinetobacter baumannii is a nosocomial pathogen which can persist in the hospital environment not only due to the acquirement of multiple antibiotic resistances, but also because of its exceptional resistance against disinfectants and desiccation. A suitable desiccation assay was established in which A. baumannii ATC...

    journal_title:MicrobiologyOpen

    pub_type: 杂志文章

    doi:10.1002/mbo3.740

    authors: Zeidler S,Müller V

    更新日期:2019-05-01 00:00:00

  • Microbial community composition in alpine lake sediments from the Hengduan Mountains.

    abstract::Microbial communities in sediments play an important role in alpine lake ecosystems. However, the microbial diversity and community composition of alpine lake sediments from the Hengduan Mountains remain largely unknown. Therefore, based on the Illumina MiSeq platform, high-throughput sequencing analysis of the 16S rR...

    journal_title:MicrobiologyOpen

    pub_type: 杂志文章

    doi:10.1002/mbo3.832

    authors: Liao B,Yan X,Zhang J,Chen M,Li Y,Huang J,Lei M,He H,Wang J

    更新日期:2019-09-01 00:00:00

  • CopM is a novel copper-binding protein involved in copper resistance in Synechocystis sp. PCC 6803.

    abstract::Copper resistance system in the cyanobacterium Synechocystis sp. PCC 6803 comprises two operons, copMRS and copBAC, which are expressed in response to copper in the media. copBAC codes for a heavy-metal efflux-resistance nodulation and division (HME-RND) system, while copMRS codes for a protein of unknown function, Co...

    journal_title:MicrobiologyOpen

    pub_type: 杂志文章

    doi:10.1002/mbo3.231

    authors: Giner-Lamia J,López-Maury L,Florencio FJ

    更新日期:2015-02-01 00:00:00

  • Comparative analysis of the composition and function of fecal-gut bacteria in captive juvenile Crocodylus siamensis between healthy and anorexic individuals.

    abstract::The Siamese crocodile (Crocodylus siamensis) is a freshwater, endangered crocodile with high economic value in the farming industry. Gut microflora plays an essential role in host physiological activity, and it contributes significantly to both the health and diseased states of animals. However, thus far, no study has...

    journal_title:MicrobiologyOpen

    pub_type: 杂志文章

    doi:10.1002/mbo3.929

    authors: Lin M,Zeng C,Li Z,Ma Y,Jia X

    更新日期:2019-12-01 00:00:00

  • Bacterial community dynamics in spontaneous sourdoughs made from wheat, spelt, and rye wholemeal flour.

    abstract::Sourdough fermentation is a traditional process that is used to improve bread quality. A spontaneous sourdough ecosystem consists of a mixture of flour and water that is fermented by endogenous lactic acid bacteria (LAB) and yeasts. The aim of this study was to identify bacterial diversity during backslopping of spont...

    journal_title:MicrobiologyOpen

    pub_type: 杂志文章

    doi:10.1002/mbo3.1009

    authors: Boreczek J,Litwinek D,Żylińska-Urban J,Izak D,Buksa K,Gawor J,Gromadka R,Bardowski JK,Kowalczyk M

    更新日期:2020-04-01 00:00:00

  • Investigating the effect of carbon source on rabies virus glycoprotein production in Pichia pastoris by a transcriptomic approach.

    abstract::Several factors affect protein expression in Pichia pastoris, one among them is the carbon source. In this work, we studied the effect of this factor on the expression level of rabies virus glycoprotein (RABV-G) in two recombinant clones harboring seven copies of the gene of interest. The expression was driven either ...

    journal_title:MicrobiologyOpen

    pub_type: 杂志文章

    doi:10.1002/mbo3.489

    authors: Ben Azoun S,Kallel H

    更新日期:2017-08-01 00:00:00

  • Identification and characterization of OmpT-like proteases in uropathogenic Escherichia coli clinical isolates.

    abstract::Bacterial colonization of the urogenital tract is limited by innate defenses, including the production of antimicrobial peptides (AMPs). Uropathogenic Escherichia coli (UPEC) resist AMP-killing to cause a range of urinary tract infections (UTIs) including asymptomatic bacteriuria, cystitis, pyelonephritis, and sepsis....

    journal_title:MicrobiologyOpen

    pub_type: 杂志文章

    doi:10.1002/mbo3.915

    authors: Desloges I,Taylor JA,Leclerc JM,Brannon JR,Portt A,Spencer JD,Dewar K,Marczynski GT,Manges A,Gruenheid S,Le Moual H,Thomassin JL

    更新日期:2019-11-01 00:00:00

  • Unique secreted-surface protein complex of Lactobacillus rhamnosus, identified by phage display.

    abstract::Proteins are the most diverse structures on bacterial surfaces; hence, they are candidates for species- and strain-specific interactions of bacteria with the host, environment, and other microorganisms. Genomics has decoded thousands of bacterial surface and secreted proteins, yet the function of most cannot be predic...

    journal_title:MicrobiologyOpen

    pub_type: 杂志文章

    doi:10.1002/mbo3.53

    authors: Gagic D,Wen W,Collett MA,Rakonjac J

    更新日期:2013-02-01 00:00:00

  • The differential susceptibility of spores from virulent and attenuated Bacillus anthracis strains to aldehyde- and hypochlorite-based disinfectants.

    abstract::This study compared the sensitivity of spores from virulent and attenuated Bacillus anthracis strains in suspension to inactivation by various chemical disinfectants. Spore suspensions from two virulent strains (A0256 and A0372) and two attenuated strains (Sterne and A0141) of B. anthracis were tested against two alde...

    journal_title:MicrobiologyOpen

    pub_type: 杂志文章

    doi:10.1002/mbo3.45

    authors: March JK,Cohen MN,Lindsey JM,Millar DA,Lowe CW,Bunnell AJ,O'Neill KL,Schaalje GB,Robison RA

    更新日期:2012-12-01 00:00:00

  • Genetic relatedness of the Escherichia coli fecal population and strains causing urinary tract infection in the same host.

    abstract::It is common knowledge that fecal microbiota is a primary source of Escherichia coli causing urinary tract infections (UTIs) via the fecal-perineal-urethral route. But, it is still unknown whether E. coli UTI is mainly caused by dominant fecal E. coli isolates (prevalence hypothesis) or the isolates that possess more ...

    journal_title:MicrobiologyOpen

    pub_type: 杂志文章

    doi:10.1002/mbo3.759

    authors: Bahadori M,Motamedifar M,Derakhshandeh A,Firouzi R,Motamedi Boroojeni A,Alinejad M,Naziri Z

    更新日期:2019-06-01 00:00:00

  • Spatial patterns in soil physicochemical and microbiological properties in a grassland adjacent to a newly built lake.

    abstract::Soil water content (SWC) is an important determinant for nutrient cycling and microorganism activity in the grassland ecosystem. Lakes have a positive effect on the water supply of the neighboring ecosystem. However, information evaluating whether newly built lakes improve the physiochemical properties and microorgani...

    journal_title:MicrobiologyOpen

    pub_type: 杂志文章

    doi:10.1002/mbo3.912

    authors: Li J,An C,Shang J,Zhao T,Zhang Q,Yang X,Ren C,Huang D,Liu K,Shao X

    更新日期:2019-12-01 00:00:00

  • Evaluation of two xenobiotic reductases from Pseudomonas putida for their suitability for magnetic nanoparticle-directed enzyme prodrug therapy as a novel approach to cancer treatment.

    abstract::Directed enzyme prodrug therapy (DEPT) is a cancer chemotherapy strategy in which bacterial enzymes are delivered to a cancer site before prodrug administration, resulting in prodrug activation at the cancer site and more localized treatment. A major limitation to DEPT is the poor effectiveness of the most studied enz...

    journal_title:MicrobiologyOpen

    pub_type: 杂志文章

    doi:10.1002/mbo3.1110

    authors: Ball P,Halliwell J,Anderson S,Gwenin V,Gwenin C

    更新日期:2020-10-01 00:00:00

  • Characterization of carotenoids in Rhodothermus marinus.

    abstract::Rhodothermus marinus, a marine aerobic thermophile, was first isolated from an intertidal hot spring in Iceland. In recent years, the R. marinus strain PRI 493 has been genetically modified, which opens up possibilities for targeted metabolic engineering of the species, such as of the carotenoid biosynthetic pathway. ...

    journal_title:MicrobiologyOpen

    pub_type: 杂志文章

    doi:10.1002/mbo3.536

    authors: Ron EYC,Plaza M,Kristjansdottir T,Sardari RRR,Bjornsdottir SH,Gudmundsson S,Hreggvidsson GO,Turner C,van Niel EWJ,Nordberg-Karlsson E

    更新日期:2018-02-01 00:00:00

  • Comparative genomics of hepatitis A virus, hepatitis C virus, and hepatitis E virus provides insights into the evolutionary history of Hepatovirus species.

    abstract::The intraspecies genomic diversity of the single-strand RNA (+) virus species hepatitis A virus (Hepatovirus), hepatitis C virus (Hepacivirus), and hepatitis E virus (Orthohepevirus) was compared. These viral species all can cause liver inflammation (hepatitis), but share no gene similarity. The codon usage of human h...

    journal_title:MicrobiologyOpen

    pub_type: 杂志文章

    doi:10.1002/mbo3.973

    authors: Wassenaar TM,Jun SR,Robeson M,Ussery DW

    更新日期:2020-02-01 00:00:00

  • Evolutionary changes of an intestinal Lactobacillus reuteri during probiotic manufacture.

    abstract::Probiotic bacteria are frequently used to treat intestinal diseases or to improve health; however, little is known about the evolutionary changes of these bacteria during probiotic manufacture and the bacterial ability to colonize the intestine. It has been observed that when bacteria adapt to a new environment, they ...

    journal_title:MicrobiologyOpen

    pub_type: 杂志文章

    doi:10.1002/mbo3.972

    authors: López L,Calderón D,Cardenas P,Prado MB,Valle C,Trueba G

    更新日期:2020-02-01 00:00:00

  • Autoinducer2 affects trimethoprim-sulfamethoxazole susceptibility in avian pathogenic Escherichia coli dependent on the folate synthesis-associate pathway.

    abstract::Avian pathogenic Escherichia coli (APEC) causes airsacculitis, polyserositis, septicemia, and other mainly extraintestinal diseases in chickens, ducks, geese, pigeons, and other avian species, and is responsible for great economic losses in the avian industry. The autoinducer 2 (AI-2) quorum sensing system is widely p...

    journal_title:MicrobiologyOpen

    pub_type: 杂志文章

    doi:10.1002/mbo3.582

    authors: Yu L,Li W,Zhang M,Cui Y,Chen X,Ni J,Yu L,Shang F,Xue T

    更新日期:2018-08-01 00:00:00