Temperature stress promotes cell division arrest in Xanthomonas citri subsp. citri.

Abstract:

:Citrus canker is an economically important disease that affects orange production in some of the most important producing areas around the world. It represents a great threat to the Brazilian and North American citriculture, particularly to the states of São Paulo and Florida, which together correspond to the biggest orange juice producers in the world. The etiological agent of this disease is the Gram-negative bacterium Xanthomonas citri subsp. citri (Xcc), which grows optimally in laboratory cultures at ~30 °C. To investigate how temperatures differing from 30 °C influence the development of Xcc, we subjected the bacterium to thermal stresses, and afterward scored its recovery capability. In addition, we analyzed cell morphology and some markers of essential cellular processes that could indicate the extent of the heat-induced damage. We found that the exposure of Xcc to 37 °C for a period of 6 h led to a cell cycle arrest at the division stage. Thermal stress might have also interfered with the DNA replication and/or the chromosome segregation apparatuses, since cells displayed an increased number of sister origins side-by-side within rods. Additionally, Xcc treated at 37 °C was still able to induce citrus canker symptoms, showing that thermal stress did not affect the ability of Xcc to colonize the host citrus. At 40-42 °C, Xcc lost viability and became unable to induce disease symptoms in citrus. Our results provide evidence about essential cellular mechanisms perturbed by temperature, and can be potentially explored as a new method for Xanthomonas citri synchronization in cell cycle studies, as well as for the sanitation of plant material.

journal_name

Microbiologyopen

journal_title

MicrobiologyOpen

authors

Sumares JA,Morão LG,Martins PM,Martins DA,Gomes E,Belasque J Jr,Ferreira H

doi

10.1002/mbo3.323

subject

Has Abstract

pub_date

2016-04-01 00:00:00

pages

244-53

issue

2

issn

2045-8827

journal_volume

5

pub_type

杂志文章
  • Selective chemoattraction of the benthic diatom Seminavis robusta to phosphate but not to inorganic nitrogen sources contributes to biofilm structuring.

    abstract::Diatoms frequently dominate marine and freshwater biofilms as major primary producers. Nutrient resources in these biofilms are patchily distributed and fluctuate dynamically over time. We recently reported that this spatially and temporally structured environment can be exploited by motile diatoms that use chemoattra...

    journal_title:MicrobiologyOpen

    pub_type: 杂志文章

    doi:10.1002/mbo3.694

    authors: V Bondoc KG,Lembke C,Vyverman W,Pohnert G

    更新日期:2019-04-01 00:00:00

  • Phage therapy treatment of the coral pathogen Vibrio coralliilyticus.

    abstract::Vibrio coralliilyticus is an important coral pathogen demonstrated to cause disease outbreaks worldwide. This study investigated the feasibility of applying bacteriophage therapy to treat the coral pathogen V. coralliilyticus. A specific bacteriophage for V. coralliilyticus strain P1 (LMG23696), referred to here as ba...

    journal_title:MicrobiologyOpen

    pub_type: 杂志文章

    doi:10.1002/mbo3.52

    authors: Cohen Y,Joseph Pollock F,Rosenberg E,Bourne DG

    更新日期:2013-02-01 00:00:00

  • Microbial community composition in alpine lake sediments from the Hengduan Mountains.

    abstract::Microbial communities in sediments play an important role in alpine lake ecosystems. However, the microbial diversity and community composition of alpine lake sediments from the Hengduan Mountains remain largely unknown. Therefore, based on the Illumina MiSeq platform, high-throughput sequencing analysis of the 16S rR...

    journal_title:MicrobiologyOpen

    pub_type: 杂志文章

    doi:10.1002/mbo3.832

    authors: Liao B,Yan X,Zhang J,Chen M,Li Y,Huang J,Lei M,He H,Wang J

    更新日期:2019-09-01 00:00:00

  • Taxonomic and functional profiles of soil samples from Atlantic forest and Caatinga biomes in northeastern Brazil.

    abstract::Although microorganisms play crucial roles in ecosystems, metagenomic analyses of soil samples are quite scarce, especially in the Southern Hemisphere. In this work, the microbial diversity of soil samples from an Atlantic Forest and Caatinga was analyzed using a metagenomic approach. Proteobacteria and Actinobacteria...

    journal_title:MicrobiologyOpen

    pub_type: 杂志文章

    doi:10.1002/mbo3.169

    authors: Pacchioni RG,Carvalho FM,Thompson CE,Faustino AL,Nicolini F,Pereira TS,Silva RC,Cantão ME,Gerber A,Vasconcelos AT,Agnez-Lima LF

    更新日期:2014-06-01 00:00:00

  • Immunogenicity of a bivalent protein as a vaccine against Edwardsiella anguillarum and Vibrio vulnificus in Japanese eel (Anguilla japonica).

    abstract::The OMPs A (OmpA)-of Edwardsiella anguillarum and OmpU of V. vulnificus have been proven to be good antigens. In this study, after construction of a vector, a new recombinant Omp (rOMP) containing both OmpA and OmpU was expressed and purified. Then, the Japanese eels (Anguilla japonica) were intraperitoneally (i.p.) i...

    journal_title:MicrobiologyOpen

    pub_type: 杂志文章

    doi:10.1002/mbo3.766

    authors: Guo S,Hu L,Feng J,Lin P,He L,Yan Q

    更新日期:2019-06-01 00:00:00

  • Metabolic engineering to expand the substrate spectrum of Pseudomonas putida toward sucrose.

    abstract::Sucrose is an important disaccharide used as a substrate in many industrial applications. It is a major component of molasses, a cheap by-product of the sugar industry. Unfortunately, not all industrially relevant organisms, among them Pseudomonas putida, are capable of metabolizing sucrose. We chose a metabolic engin...

    journal_title:MicrobiologyOpen

    pub_type: 杂志文章

    doi:10.1002/mbo3.473

    authors: Löwe H,Schmauder L,Hobmeier K,Kremling A,Pflüger-Grau K

    更新日期:2017-08-01 00:00:00

  • XatA, an AT-1 autotransporter important for the virulence of Xylella fastidiosa Temecula1.

    abstract::Xylella fastidiosa Temecula1 is the causative agent of Pierce's disease of grapevine, which is spread by xylem-feeding insects. An important feature of the infection cycle is the ability of X. fastidiosa to colonize and interact with two distinct environments, the xylem of susceptible plants and the insect foregut. He...

    journal_title:MicrobiologyOpen

    pub_type: 杂志文章

    doi:10.1002/mbo3.6

    authors: Matsumoto A,Huston SL,Killiny N,Igo MM

    更新日期:2012-03-01 00:00:00

  • The effect of sulfamonomethoxine treatment on the gut microbiota of Nile tilapia (Oreochromis niloticus).

    abstract::To investigate the possible effects of sulfamonomethoxine (SMM) on Nile tilapia (Oreochromis niloticus), we quantitatively evaluated the microbial shifts in the intestines of Nile tilapia in response to different doses of SMM (200 and 300 mg/kg) using 16S rRNA gene sequencing. At the phylum level, the control group (0...

    journal_title:MicrobiologyOpen

    pub_type: 杂志文章

    doi:10.1002/mbo3.1116

    authors: Ming J,Fu Z,Ma Z,Zhou L,Zhang Z,Song C,Yuan X,Wu Q

    更新日期:2020-11-01 00:00:00

  • Bacterial community dynamics in spontaneous sourdoughs made from wheat, spelt, and rye wholemeal flour.

    abstract::Sourdough fermentation is a traditional process that is used to improve bread quality. A spontaneous sourdough ecosystem consists of a mixture of flour and water that is fermented by endogenous lactic acid bacteria (LAB) and yeasts. The aim of this study was to identify bacterial diversity during backslopping of spont...

    journal_title:MicrobiologyOpen

    pub_type: 杂志文章

    doi:10.1002/mbo3.1009

    authors: Boreczek J,Litwinek D,Żylińska-Urban J,Izak D,Buksa K,Gawor J,Gromadka R,Bardowski JK,Kowalczyk M

    更新日期:2020-04-01 00:00:00

  • Genetic determinants of swimming motility in the squid light-organ symbiont Vibrio fischeri.

    abstract::Bacterial flagellar motility is a complex cellular behavior required for the colonization of the light-emitting organ of the Hawaiian bobtail squid, Euprymna scolopes, by the beneficial bioluminescent symbiont Vibrio fischeri. We characterized the basis of this behavior by performing (i) a forward genetic screen to id...

    journal_title:MicrobiologyOpen

    pub_type: 杂志文章

    doi:10.1002/mbo3.96

    authors: Brennan CA,Mandel MJ,Gyllborg MC,Thomasgard KA,Ruby EG

    更新日期:2013-08-01 00:00:00

  • Assessment of carbapenem-resistant Enterobacteriaceae-plate formula and quality control procedure.

    abstract:AIMS:To assess a cost-effective in-house selective plate formula for actively screening carbapenem-resistant Enterobacteriaceae (CRE). METHODOLOGY AND RESULTS:The in-house formula included CHROMagarTM Orientation, meropenem, and ingredients present in the Mac-Conkey formula, such as bile salts and crystal violet (pH 6...

    journal_title:MicrobiologyOpen

    pub_type: 杂志文章

    doi:10.1002/mbo3.1130

    authors: Tran TC,Pham BT,Pham VH,Ngo TA,Hanberger H,Larsson M,Olson L

    更新日期:2020-12-01 00:00:00

  • UV Resistance of bacteria from the Kenyan Marine cyanobacterium Moorea producens.

    abstract::UV resistance of bacteria isolated from the marine cyanobacterium Moorea producens has not been observed previously, findings which highlight how unsafe germicidal UV irradiation for sterilization of air, food, and water could be. Further, UV resistance of Bacillus licheniformis is being observed for the first time. T...

    journal_title:MicrobiologyOpen

    pub_type: 杂志文章

    doi:10.1002/mbo3.697

    authors: Dzeha T,Nyiro C,Kardasopoulos D,Mburu D,Mwafaida J,Hall MJ,Burgess JG

    更新日期:2019-04-01 00:00:00

  • Biosynthetic approach to combine the first steps of cardenolide formation in Saccharomyces cerevisiae.

    abstract::A yeast expression plasmid was constructed containing a cardenolide biosynthetic module, referred to as CARD II, using the AssemblX toolkit, which enables the assembly of large DNA constructs. The genes cloned into the vector were (a) a Δ5 -3β-hydroxysteroid dehydrogenase gene from Digitalis lanata, (b) a steroid Δ5 -...

    journal_title:MicrobiologyOpen

    pub_type: 杂志文章

    doi:10.1002/mbo3.925

    authors: Rieck C,Geiger D,Munkert J,Messerschmidt K,Petersen J,Strasser J,Meitinger N,Kreis W

    更新日期:2019-12-01 00:00:00

  • UV-resistant yeasts isolated from a high-altitude volcanic area on the Atacama Desert as eukaryotic models for astrobiology.

    abstract::The Sairecabur volcano (5971 m), in the Atacama Desert, is a high-altitude extreme environment with high daily temperature variations, acidic soils, intense UV radiation, and low availability of water. Four different species of yeasts were isolated from this region using oligotrophic media, identified and characterize...

    journal_title:MicrobiologyOpen

    pub_type: 杂志文章

    doi:10.1002/mbo3.262

    authors: Pulschen AA,Rodrigues F,Duarte RT,Araujo GG,Santiago IF,Paulino-Lima IG,Rosa CA,Kato MJ,Pellizari VH,Galante D

    更新日期:2015-08-01 00:00:00

  • The differential susceptibility of spores from virulent and attenuated Bacillus anthracis strains to aldehyde- and hypochlorite-based disinfectants.

    abstract::This study compared the sensitivity of spores from virulent and attenuated Bacillus anthracis strains in suspension to inactivation by various chemical disinfectants. Spore suspensions from two virulent strains (A0256 and A0372) and two attenuated strains (Sterne and A0141) of B. anthracis were tested against two alde...

    journal_title:MicrobiologyOpen

    pub_type: 杂志文章

    doi:10.1002/mbo3.45

    authors: March JK,Cohen MN,Lindsey JM,Millar DA,Lowe CW,Bunnell AJ,O'Neill KL,Schaalje GB,Robison RA

    更新日期:2012-12-01 00:00:00

  • Acetylome analysis of lysine acetylation in the plant pathogenic bacterium Brenneria nigrifluens.

    abstract::Protein lysine acetylation, a dynamic and reversible posttranslational modification, plays a crucial role in several cellular processes, including cell cycle regulation, metabolism, enzymatic activities, and protein interactions. Brenneria nigrifluens is a pathogen of walnut trees with shallow bark canker and can caus...

    journal_title:MicrobiologyOpen

    pub_type: 杂志文章

    doi:10.1002/mbo3.952

    authors: Li Y,Xue H,Bian DR,Xu G,Piao C

    更新日期:2020-01-01 00:00:00

  • The PAS domains of the major sporulation kinase in Bacillus subtilis play a role in tetramer formation that is essential for the autokinase activity.

    abstract::Sporulation in Bacillus subtilis is induced upon starvation. In a widely accepted model, an N-terminal "sensor" domain of the major sporulation kinase KinA recognizes a hypothetical starvation signal(s) and autophosphorylates a histidine residue to activate the master regulator Spo0A via a multicomponent phosphorelay....

    journal_title:MicrobiologyOpen

    pub_type: 杂志文章

    doi:10.1002/mbo3.481

    authors: Kiehler B,Haggett L,Fujita M

    更新日期:2017-08-01 00:00:00

  • Microbial composition of enigmatic bird parasites: Wolbachia and Spiroplasma are the most important bacterial associates of quill mites (Acariformes: Syringophilidae).

    abstract:BACKGROUND:The microbiome is an integral component of many animal species, potentially affecting behavior, physiology, and other biological properties. Despite this importance, bacterial communities remain vastly understudied in many groups of invertebrates, including mites. Quill mites (Acariformes: Syringophilidae) a...

    journal_title:MicrobiologyOpen

    pub_type: 杂志文章

    doi:10.1002/mbo3.964

    authors: Glowska E,Filutowska ZK,Dabert M,Gerth M

    更新日期:2020-05-01 00:00:00

  • Mcr colistin resistance gene: a systematic review of current diagnostics and detection methods.

    abstract::Resistance to colistin, mediated by chromosomal mutations and more recently, by plasmid-borne mcr genes, is increasingly being reported in bacterial isolates taken from humans, animals, farms, foods, and the environment. To easily identify and contain this quickly spreading menace, efficient diagnostics that are cheap...

    journal_title:MicrobiologyOpen

    pub_type: 杂志文章

    doi:10.1002/mbo3.682

    authors: Osei Sekyere J

    更新日期:2019-04-01 00:00:00

  • The DNABII family of proteins is comprised of the only nucleoid associated proteins required for nontypeable Haemophilus influenzae biofilm structure.

    abstract::Biofilms play a central role in the pathobiology of otitis media (OM), bronchitis, sinusitis, conjunctivitis, and pneumonia caused by nontypeable Haemophilus influenzae (NTHI). Our previous studies show that extracellular DNA (eDNA) and DNABII proteins are essential components of biofilms formed by NTHI. The DNABII pr...

    journal_title:MicrobiologyOpen

    pub_type: 杂志文章

    doi:10.1002/mbo3.563

    authors: Devaraj A,Buzzo J,Rocco CJ,Bakaletz LO,Goodman SD

    更新日期:2018-06-01 00:00:00

  • Truncation of type IV pilin induces mucoidy in Pseudomonas aeruginosa strain PAO579.

    abstract::Pseudomonas aeruginosa is a Gram negative, opportunistic pathogen that uses the overproduction of alginate, a surface polysaccharide, to form biofilms in vivo. Overproduction of alginate, also known as mucoidy, affords the bacterium protection from the host's defenses and facilitates the establishment of chronic lung ...

    journal_title:MicrobiologyOpen

    pub_type: 杂志文章

    doi:10.1002/mbo3.86

    authors: Ryan Withers T,Heath Damron F,Yin Y,Yu HD

    更新日期:2013-06-01 00:00:00

  • Evaluation of DNA extraction kits and phylogenetic diversity of the porcine gastrointestinal tract based on Illumina sequencing of two hypervariable regions.

    abstract::A robust DNA extraction method is important to identify the majority of microorganisms present in environmental microbial communities and to enable a consistent comparison between different studies. Here, 15 manual and four automated commercial DNA extraction kits were evaluated for their efficiency to extract DNA fro...

    journal_title:MicrobiologyOpen

    pub_type: 杂志文章

    doi:10.1002/mbo3.312

    authors: Burbach K,Seifert J,Pieper DH,Camarinha-Silva A

    更新日期:2016-02-01 00:00:00

  • Immunity protein release from a cell-bound nuclease colicin complex requires global conformational rearrangement.

    abstract::Nuclease colicins bind their target receptor BtuB in the outer membrane of sensitive Escherichia coli cells in the form of a high-affinity complex with their cognate immunity proteins. The release of the immunity protein from the colicin complex is a prerequisite for cell entry of the colicin and occurs via a process ...

    journal_title:MicrobiologyOpen

    pub_type: 杂志文章

    doi:10.1002/mbo3.122

    authors: Vankemmelbeke M,Housden NG,James R,Kleanthous C,Penfold CN

    更新日期:2013-10-01 00:00:00

  • Effect of copper ion and soil humic acid on biodegradation of decabromodiphenyl ether (BDE-209) by Pseudomonas aeruginosa.

    abstract::Pseudomonas aeruginosa is a good environmental microorganism capable of degrading decabromodiphenyl ether (BDE-209). This paper studied the effect of Cu2+ and humic acid (HA) extracted from e-waste contaminated soils on biodegradation of BDE-209 by P. aeruginosa. The adsorption isotherms of Cu2+ on HA, the crude enzym...

    journal_title:MicrobiologyOpen

    pub_type: 杂志文章

    doi:10.1002/mbo3.439

    authors: Liu Y,Gong A,Qiu L,Li J,Li F

    更新日期:2017-06-01 00:00:00

  • Investigating the effect of carbon source on rabies virus glycoprotein production in Pichia pastoris by a transcriptomic approach.

    abstract::Several factors affect protein expression in Pichia pastoris, one among them is the carbon source. In this work, we studied the effect of this factor on the expression level of rabies virus glycoprotein (RABV-G) in two recombinant clones harboring seven copies of the gene of interest. The expression was driven either ...

    journal_title:MicrobiologyOpen

    pub_type: 杂志文章

    doi:10.1002/mbo3.489

    authors: Ben Azoun S,Kallel H

    更新日期:2017-08-01 00:00:00

  • Hexyl gallate for the control of citrus canker caused by Xanthomonas citri subsp citri.

    abstract::Brazil is the biggest producer of sweet oranges and the main exporter of concentrated orange juice in the world. Among the diseases that affect citriculture, Asiatic citrus canker, caused by the bacterial pathogen Xanthomonas citri, represents one of the most significant threats. The current Brazilian legislation regu...

    journal_title:MicrobiologyOpen

    pub_type: 杂志文章

    doi:10.1002/mbo3.1104

    authors: Cavalca LB,Zamuner CFC,Saldanha LL,Polaquini CR,Regasini LO,Behlau F,Ferreira H

    更新日期:2020-09-01 00:00:00

  • A novel gingipain regulatory gene in Porphyromonas gingivalis mediates host cell detachment and inhibition of wound closure.

    abstract::The black pigmentation-related genes in Porphyromonas gingivalis are primarily involved in regulating gingipain functions. In this study, we identified a pigmentation-related gene, designated as pgn_0361. To characterize the role of pgn_0361 in regulating P. gingivalis-mediated epithelial cell detachment and inhibitio...

    journal_title:MicrobiologyOpen

    pub_type: 杂志文章

    doi:10.1002/mbo3.1128

    authors: Liu H,Huang L,Cai Y,Bikker FJ,Wei X,Mei Deng D

    更新日期:2020-12-01 00:00:00

  • Investigating cross-contamination by yeast strains from dental solid waste to waste-handling workers by DNA sequencing.

    abstract::Trying to widen the discussion on the risks associated with dental waste, this study proposed to investigate and genetically compare yeast isolates recovered from dental solid waste and waste workers. Three samples were collected from workers' hands, nasal mucosa, and professional clothing (days 0, 30, and 180), and t...

    journal_title:MicrobiologyOpen

    pub_type: 杂志文章

    doi:10.1002/mbo3.554

    authors: Vieira CD,Tagliaferri TL,de Carvalho MAR,de Resende-Stoianoff MA,Holanda RA,de Magalhães TFF,Magalhães PP,Dos Santos SG,de Macêdo Farias L

    更新日期:2018-04-01 00:00:00

  • Comparative analysis of the composition and function of fecal-gut bacteria in captive juvenile Crocodylus siamensis between healthy and anorexic individuals.

    abstract::The Siamese crocodile (Crocodylus siamensis) is a freshwater, endangered crocodile with high economic value in the farming industry. Gut microflora plays an essential role in host physiological activity, and it contributes significantly to both the health and diseased states of animals. However, thus far, no study has...

    journal_title:MicrobiologyOpen

    pub_type: 杂志文章

    doi:10.1002/mbo3.929

    authors: Lin M,Zeng C,Li Z,Ma Y,Jia X

    更新日期:2019-12-01 00:00:00

  • Influence of agro-environmental pollutants on a biocontrol strain of Bacillus velezensis.

    abstract::Metal- and pesticide-tolerant biocontrol agents are preferred in integrated pest management, as such strains can be applied in combination with different pesticides. The Bacillus velezensis strain SZMC 6161J proved to be sensitive to copper, nickel, zinc, and cadmium, while manganese elevated its growth. At concentrat...

    journal_title:MicrobiologyOpen

    pub_type: 杂志文章

    doi:10.1002/mbo3.660

    authors: Vörös M,Manczinger L,Kredics L,Szekeres A,Shine K,Alharbi NS,Khaled JM,Vágvölgyi C

    更新日期:2019-03-01 00:00:00