Abstract:
:Marine picocyanobacteria belonging to genera Synechococcus and Prochlorococcus are genetically diverged and distributed into distinct biogeographical patterns, and both are infected by genetically closely related cyanopodoviruses. Previous studies have not fully explored whether the two virus-host systems share similar gene expression patterns during infection. Whole-genome expression dynamics of T7-like cyanopodovirus P-SSP7 and its host Prochlorococcus strain MED4 have already been reported. Here, we conducted genomic and transcriptomic analyses on T7-like cyanopodovirus S-SBP1 during its infection on Synechococcus strain WH7803. S-SBP1 has a latent period of 8 h and phage DNA production of 30 copies per cell. In terms of whole-genome phylogenetic relationships and average nucleotide identity, S-SBP1 was most similar to cyanopodovirus S-RIP2, which also infects Synechococcus WH7803. Three hypervariable genomic islands were identified when comparing the genomes of S-SBP1 and S-RIP2. Single nucleotide variants were also observed in three S-SBP1 genes, which were located within the island regions. Based on RNA-seq analysis, S-SBP1 genes clustered into three temporal expression classes, whose gene content was similar to that of P-SSP7. Thirty-two host genes were upregulated during phage infection, including those involved in carbon metabolism, ribosome components, and stress response. These upregulated genes were similar to those upregulated by Prochlorococcus MED4 in response to infection by P-SSP7. Our study demonstrates a programmed temporal expression pattern of cyanopodoviruses and hosts during infection.
journal_name
Microbiologyopenjournal_title
MicrobiologyOpenauthors
Huang S,Sun Y,Zhang S,Long Ldoi
10.1002/mbo3.1150subject
Has Abstractpub_date
2020-12-30 00:00:00pages
e1150issn
2045-8827pub_type
杂志文章相关文献
MicrobiologyOpen文献大全abstract::Extracts from white birch have been reported to possess antimicrobial properties, but no study has linked the chemical composition of bark extract with antimicrobial activity. This study aimed to identify white birch (Betula papyrifera Marshall) bark extracts with antimicrobial activity and elucidate its composition. ...
journal_title:MicrobiologyOpen
pub_type: 杂志文章
doi:10.1002/mbo3.944
更新日期:2020-01-01 00:00:00
abstract::[NiFe]-hydrogenases catalyze the reversible conversion of molecular hydrogen into protons end electrons. This reaction takes place at a NiFe(CN)2 (CO) cofactor located in the large subunit of the bipartite hydrogenase module. The corresponding apo-protein carries usually a C-terminal extension that is cleaved off by a...
journal_title:MicrobiologyOpen
pub_type: 杂志文章
doi:10.1002/mbo3.1029
更新日期:2020-06-01 00:00:00
abstract::Kalimantacin is an antimicrobial compound with strong antistaphylococcal activity that is produced by a hybrid trans-acyltransferase polyketide synthase/nonribosomal peptide synthetase system in Pseudomonas fluorescens BCCM_ID9359. We here present a systematic analysis of the substrate specificity of the glycine-incor...
journal_title:MicrobiologyOpen
pub_type: 杂志文章
doi:10.1002/mbo3.326
更新日期:2016-04-01 00:00:00
abstract::Microbially mediated decomposition of particulate organic carbon (POC) is a central component of the oceanic carbon cycle, controlling the flux of organic carbon from the surface ocean to the deep ocean. Yet, the specific microbial taxa responsible for POC decomposition and degradation in the deep ocean are still unkn...
journal_title:MicrobiologyOpen
pub_type: 杂志文章
doi:10.1002/mbo3.1013
更新日期:2020-05-01 00:00:00
abstract::In rod-shaped bacteria, the proper placement of the division septum at the midcell relies, at least partially, on the proteins of the Min system as an inhibitor of cell division. The main principle of Min system function involves the formation of an inhibitor gradient along the cell axis; however, the establishment of...
journal_title:MicrobiologyOpen
pub_type: 杂志文章
doi:10.1002/mbo3.337
更新日期:2016-06-01 00:00:00
abstract::The Sairecabur volcano (5971 m), in the Atacama Desert, is a high-altitude extreme environment with high daily temperature variations, acidic soils, intense UV radiation, and low availability of water. Four different species of yeasts were isolated from this region using oligotrophic media, identified and characterize...
journal_title:MicrobiologyOpen
pub_type: 杂志文章
doi:10.1002/mbo3.262
更新日期:2015-08-01 00:00:00
abstract::Aflatoxin M1 (AFM1 ) is a potent mycotoxin which causes serious health concerns in developing countries, where it is mainly found in milk, meat, and other foods. Biological detoxification is a promising method for eliminating AFM1 . The aim of this work was to search for AFM1 -degrading bacterial strains from animal w...
journal_title:MicrobiologyOpen
pub_type: 杂志文章
doi:10.1002/mbo3.663
更新日期:2019-03-01 00:00:00
abstract::The Hatton-Rockall Basin (North-East Atlantic) is an area with potential for deep-sea (2,900 m) hydrocarbon exploration. Following the Deepwater Horizon oil spill, many investigations into the responses of sediment microbial communities to oil pollution have been undertaken. However, hydrostatic pressure is a paramete...
journal_title:MicrobiologyOpen
pub_type: 杂志文章
doi:10.1002/mbo3.768
更新日期:2019-06-01 00:00:00
abstract::Dinitrogen fixation, the biological reduction in N2 gas to ammonia contributes to the supply of new nitrogen in the surface ocean. To understand the diversity and abundance of potentially diazotrophic (N2 fixing) microorganisms associated with marine zooplankton, especially copepods, the nifH gene was studied using zo...
journal_title:MicrobiologyOpen
pub_type: 杂志文章
doi:10.1002/mbo3.385
更新日期:2016-12-01 00:00:00
abstract::The OMPs A (OmpA)-of Edwardsiella anguillarum and OmpU of V. vulnificus have been proven to be good antigens. In this study, after construction of a vector, a new recombinant Omp (rOMP) containing both OmpA and OmpU was expressed and purified. Then, the Japanese eels (Anguilla japonica) were intraperitoneally (i.p.) i...
journal_title:MicrobiologyOpen
pub_type: 杂志文章
doi:10.1002/mbo3.766
更新日期:2019-06-01 00:00:00
abstract::The black pigmentation-related genes in Porphyromonas gingivalis are primarily involved in regulating gingipain functions. In this study, we identified a pigmentation-related gene, designated as pgn_0361. To characterize the role of pgn_0361 in regulating P. gingivalis-mediated epithelial cell detachment and inhibitio...
journal_title:MicrobiologyOpen
pub_type: 杂志文章
doi:10.1002/mbo3.1128
更新日期:2020-12-01 00:00:00
abstract::The objective of this study was to phylogenetically analyze microorganisms from the domains Bacteria and Archaea in hypersaline sediment from Death Valley National Park. Using domain-specific primers, a region of the 16S rRNA gene was amplified using polymerase chain reaction (PCR), and the product was subsequently us...
journal_title:MicrobiologyOpen
pub_type: 杂志文章
doi:10.1002/mbo3.20
更新日期:2012-06-01 00:00:00
abstract::Most DNA assembly methods require bacterial amplification steps, which restrict its application to genes that can be cloned in the bacterial host without significant toxic effects. However, genes that cannot be cloned in bacteria do not necessarily exert toxic effects on the final host. In order to tackle this issue, ...
journal_title:MicrobiologyOpen
pub_type: 杂志文章
doi:10.1002/mbo3.978
更新日期:2020-03-01 00:00:00
abstract::FtsZ - a prokaryotic tubulin homolog - is one of the central components of bacterial division machinery. At the early stage of cytokinesis FtsZ forms the so-called Z-ring at mid-cell that guides septum formation. Many approaches were used to resolve the structure of the Z-ring, however, researchers are still far from ...
journal_title:MicrobiologyOpen
pub_type: 杂志文章
doi:10.1002/mbo3.336
更新日期:2016-06-01 00:00:00
abstract::The emerging pollutants antibiotic resistance genes (ARGs) are prevalent in aquatic environments such as estuary. Coastal mangrove ecosystems always serve as natural wetlands for receiving sewage which always carry ARGs. Currently, the research considering ARG distribution in mangrove ecosystems gains more interest. I...
journal_title:MicrobiologyOpen
pub_type: 杂志文章
doi:10.1002/mbo3.871
更新日期:2019-11-01 00:00:00
abstract::In microbes that fix nitrogen, nitrogenase catalyzes the conversion of N2 to ammonia in an ATP-demanding reaction. To help conserve energy some bacteria inhibit nitrogenase activity upon exposure to ammonium. The purple nonsulfur phototrophic bacterium Rhodopseudomonas palustris strain CGA009 can synthesize three func...
journal_title:MicrobiologyOpen
pub_type: 杂志文章
doi:10.1002/mbo3.265
更新日期:2015-08-01 00:00:00
abstract::Bacterial colonization of the urogenital tract is limited by innate defenses, including the production of antimicrobial peptides (AMPs). Uropathogenic Escherichia coli (UPEC) resist AMP-killing to cause a range of urinary tract infections (UTIs) including asymptomatic bacteriuria, cystitis, pyelonephritis, and sepsis....
journal_title:MicrobiologyOpen
pub_type: 杂志文章
doi:10.1002/mbo3.915
更新日期:2019-11-01 00:00:00
abstract::A new type of structural extracellular polymers (EPS) was extracted from aerobic granular sludge dominated by ammonium-oxidizing bacteria. It was analyzed by Raman and FTIR spectroscopy to characterize specific amino acids and protein secondary structure, and by SDS-PAGE with different stains to identify different gly...
journal_title:MicrobiologyOpen
pub_type: 杂志文章
doi:10.1002/mbo3.616
更新日期:2018-12-01 00:00:00
abstract::UV resistance of bacteria isolated from the marine cyanobacterium Moorea producens has not been observed previously, findings which highlight how unsafe germicidal UV irradiation for sterilization of air, food, and water could be. Further, UV resistance of Bacillus licheniformis is being observed for the first time. T...
journal_title:MicrobiologyOpen
pub_type: 杂志文章
doi:10.1002/mbo3.697
更新日期:2019-04-01 00:00:00
abstract::Sustained monoculture often leads to the inhibition of plant growth, the decrease of the soil microbial diversity, and changes in soil microbial community composition, particularly to the accumulation of soil-borne pathogens. In this study, we conducted field experiments to investigate the practical effects of tilling...
journal_title:MicrobiologyOpen
pub_type: 杂志文章
doi:10.1002/mbo3.1045
更新日期:2020-07-01 00:00:00
abstract::Sourdough fermentation is a traditional process that is used to improve bread quality. A spontaneous sourdough ecosystem consists of a mixture of flour and water that is fermented by endogenous lactic acid bacteria (LAB) and yeasts. The aim of this study was to identify bacterial diversity during backslopping of spont...
journal_title:MicrobiologyOpen
pub_type: 杂志文章
doi:10.1002/mbo3.1009
更新日期:2020-04-01 00:00:00
abstract::Trying to widen the discussion on the risks associated with dental waste, this study proposed to investigate and genetically compare yeast isolates recovered from dental solid waste and waste workers. Three samples were collected from workers' hands, nasal mucosa, and professional clothing (days 0, 30, and 180), and t...
journal_title:MicrobiologyOpen
pub_type: 杂志文章
doi:10.1002/mbo3.554
更新日期:2018-04-01 00:00:00
abstract::As part of the culturomics project aiming at describing the human microbiota, we report in this study the description of the new bacterial genus Raoultibacter gen. nov. that includes two new species, that is, R. massiliensis sp. nov. and R. timonensis sp. nov. The R. massiliensis type strain Marseille-P2849T was isola...
journal_title:MicrobiologyOpen
pub_type: 杂志文章
doi:10.1002/mbo3.758
更新日期:2019-06-01 00:00:00
abstract::Microbial biofilms are communities of surface-adhered cells enclosed in a matrix of extracellular polymeric substances. Bacterial cells in biofilm are 10~1,000-fold more resistant to antimicrobials than the planktonic cells. Burgeoning antibiotic resistance in Pseudomonas aeruginosa biofilm has necessitated the develo...
journal_title:MicrobiologyOpen
pub_type: 杂志文章
doi:10.1002/mbo3.545
更新日期:2018-02-01 00:00:00
abstract::The aim of this study was to determine the serogroups, antimicrobial resistance and genetic diversity of Escherichia coli isolates from samples of bivalve mollusks collected along Santa Catarina coast, Brazil, and from the Chesapeake Bay, Maryland, USA. One hundred forty-one E. coli isolates were characterized for ser...
journal_title:MicrobiologyOpen
pub_type: 杂志文章
doi:10.1002/mbo3.738
更新日期:2019-05-01 00:00:00
abstract::Few studies have evaluated the trophic level in tropical caves, and none related the microbial biomass dynamics in the immobilization of carbon and nitrogen. Here, four tropical caves of Terra Ronca State Park, Brazil, were studied: Angélica, São Bernardo, Terra Ronca I, and Terra Ronca II caves. Physical, chemical, a...
journal_title:MicrobiologyOpen
pub_type: 杂志文章
doi:10.1002/mbo3.1044
更新日期:2020-07-01 00:00:00
abstract::Sporulation in Bacillus subtilis is induced upon starvation. In a widely accepted model, an N-terminal "sensor" domain of the major sporulation kinase KinA recognizes a hypothetical starvation signal(s) and autophosphorylates a histidine residue to activate the master regulator Spo0A via a multicomponent phosphorelay....
journal_title:MicrobiologyOpen
pub_type: 杂志文章
doi:10.1002/mbo3.481
更新日期:2017-08-01 00:00:00
abstract::Newcastle disease, caused by virulent strain of Newcastle disease virus (NDV), is an acute, highly contagious disease that is prevalent worldwide and is responsible for serious economic losses to the poultry industry. In the current study, we compared the early immune responses in chickens infected with two strains of...
journal_title:MicrobiologyOpen
pub_type: 杂志文章
doi:10.1002/mbo3.701
更新日期:2019-04-01 00:00:00
abstract::Bacterial community structures were evaluated in oil samples using culture-independent pyrosequencing, including oil mousses collected on sea surface and salt marshes during the Deepwater Horizon oil spill, and oil deposited in sediments adjacent to the wellhead 1 year after the spill. Phylogenetic analysis suggested ...
journal_title:MicrobiologyOpen
pub_type: 杂志文章
doi:10.1002/mbo3.89
更新日期:2013-06-01 00:00:00
abstract::The Siamese crocodile (Crocodylus siamensis) is a freshwater, endangered crocodile with high economic value in the farming industry. Gut microflora plays an essential role in host physiological activity, and it contributes significantly to both the health and diseased states of animals. However, thus far, no study has...
journal_title:MicrobiologyOpen
pub_type: 杂志文章
doi:10.1002/mbo3.929
更新日期:2019-12-01 00:00:00