Evaluation of DNA extraction kits and phylogenetic diversity of the porcine gastrointestinal tract based on Illumina sequencing of two hypervariable regions.

abstract：:Methicillin-resistant S. aureus has been reported as the main pathogen involved in chronic infections, osteomyelitis, and prosthetic joint infections. The host/pathogen interaction is dynamic and requires several changes to promote bacterial survival. Here, we focused on the internalization and persistence behavior of...

journal_title：MicrobiologyOpen

authors： Bongiorno D,Musso N,Lazzaro LM,Mongelli G,Stefani S,Campanile F

更新日期：2020-05-01 00:00:00

abstract：:To investigate the possible effects of sulfamonomethoxine (SMM) on Nile tilapia (Oreochromis niloticus), we quantitatively evaluated the microbial shifts in the intestines of Nile tilapia in response to different doses of SMM (200 and 300 mg/kg) using 16S rRNA gene sequencing. At the phylum level, the control group (0...

journal_title：MicrobiologyOpen

authors： Ming J,Fu Z,Ma Z,Zhou L,Zhang Z,Song C,Yuan X,Wu Q

更新日期：2020-11-01 00:00:00

abstract：:Urinary tract infections (UTIs) are among the most common bacterial infections in humans. Although a number of bacteria can cause UTIs, most cases are due to infection by uropathogenic Escherichia coli (UPEC). UPEC are a genetically heterogeneous group that exhibit several virulence factors associated with colonizatio...

journal_title：MicrobiologyOpen

authors： Schifano E,Marazzato M,Ammendolia MG,Zanni E,Ricci M,Comanducci A,Goldoni P,Conte MP,Uccelletti D,Longhi C

更新日期：2019-06-01 00:00:00

abstract：:Microbially mediated decomposition of particulate organic carbon (POC) is a central component of the oceanic carbon cycle, controlling the flux of organic carbon from the surface ocean to the deep ocean. Yet, the specific microbial taxa responsible for POC decomposition and degradation in the deep ocean are still unkn...

journal_title：MicrobiologyOpen

authors： Liu Y,Fang J,Jia Z,Chen S,Zhang L,Gao W

更新日期：2020-05-01 00:00:00

abstract：:The interactions of pathogens and phagocytes are complex. Our study demonstrated that Aeromonas hydrophila B11 can survive in the macrophagocytes of Tilapia mossambica. To explore the regulatory processes of A. hydrophila survival in the macrophagocytes, we used the mini-Tn10 transposon mutagenesis system to build a m...

journal_title：MicrobiologyOpen

authors： Jiang Q,Chen W,Qin Y,Huang L,Xu X,Zhao L,Yan Q

更新日期：2017-08-01 00:00:00

abstract：:The composition and function of the microbial community in the gastrointestinal tract (GIT) have increasingly captured the attention of nutritionists because these traits affect the nutrient utilization efficiency and health of host animals. Little information has been reported on these aspects of the goat GIT. This s...

journal_title：MicrobiologyOpen

authors： Wang L,Jin L,Xue B,Wang Z,Peng Q

更新日期：2019-09-01 00:00:00

abstract：:Gene expression in Haloarcula hispanica cells infected with the gammapleolipovirus His2 was studied using a custom DNA microarray. Total RNA from cells sampled at 0, 1, 2, 3, and 4.5 hr postinfection was reverse-transcribed into labeled cDNA and hybridized to microarrays, revealing temporal and differential expression...

journal_title：MicrobiologyOpen

authors： Lee STM,Ding JY,Chiang PW,Dyall-Smith M,Tang SL

更新日期：2020-05-01 00:00:00

abstract：:In our previous work, we demonstrated that viable but nonculturable (VBNC) Vibrio cholerae O1 and O139 were converted to culturable by coculture with eukaryotic cells. Furthermore, we isolated a factor converting VBNC V. cholerae to culturable (FCVC) from a eukaryotic cell line, HT-29. In this study, we purified FCVC ...

journal_title：MicrobiologyOpen

authors： Senoh M,Hamabata T,Takeda Y

更新日期：2015-08-01 00:00:00

abstract：:Microorganisms require a motility structure to move towards optimal growth conditions. The motility structure from archaea, the archaellum, is fundamentally different from its bacterial counterpart, the flagellum, and is assembled in a similar fashion as type IV pili. The archaellum filament consists of thousands of c...

journal_title：MicrobiologyOpen

authors： Syutkin AS,van Wolferen M,Surin AK,Albers SV,Pyatibratov MG,Fedorov OV,Quax TEF

更新日期：2019-05-01 00:00:00

abstract：:Despite the relevance of landscape, regarding the spatial patterning of microbial communities and the relative influence of environmental parameters versus human activities, few investigations have been conducted at this scale. Here, we used a systematic grid to characterize the distribution of soil microbial communit...

journal_title：MicrobiologyOpen

authors： Constancias F,Terrat S,Saby NP,Horrigue W,Villerd J,Guillemin JP,Biju-Duval L,Nowak V,Dequiedt S,Ranjard L,Chemidlin Prévost-Bouré N

更新日期：2015-06-01 00:00:00

abstract：:Increasing popularity of preservative-free cosmetics necessitates in-depth research, specifically as bacteria can react to local factors by important metabolic changes. In this respect, investigating the effect of cosmetic preparations on pathogenic strains of commensal species such as acneic forms of Cutibacterium ac...

journal_title：MicrobiologyOpen

authors： Gannesen AV,Borrel V,Lefeuvre L,Netrusov AI,Plakunov VK,Feuilloley MGJ

更新日期：2019-03-01 00:00:00

abstract：:Copper resistance system in the cyanobacterium Synechocystis sp. PCC 6803 comprises two operons, copMRS and copBAC, which are expressed in response to copper in the media. copBAC codes for a heavy-metal efflux-resistance nodulation and division (HME-RND) system, while copMRS codes for a protein of unknown function, Co...

journal_title：MicrobiologyOpen

authors： Giner-Lamia J,López-Maury L,Florencio FJ

更新日期：2015-02-01 00:00:00

abstract：:Bacterial colonization of the urogenital tract is limited by innate defenses, including the production of antimicrobial peptides (AMPs). Uropathogenic Escherichia coli (UPEC) resist AMP-killing to cause a range of urinary tract infections (UTIs) including asymptomatic bacteriuria, cystitis, pyelonephritis, and sepsis....

journal_title：MicrobiologyOpen

authors： Desloges I,Taylor JA,Leclerc JM,Brannon JR,Portt A,Spencer JD,Dewar K,Marczynski GT,Manges A,Gruenheid S,Le Moual H,Thomassin JL

更新日期：2019-11-01 00:00:00

abstract：:In microbes that fix nitrogen, nitrogenase catalyzes the conversion of N2 to ammonia in an ATP-demanding reaction. To help conserve energy some bacteria inhibit nitrogenase activity upon exposure to ammonium. The purple nonsulfur phototrophic bacterium Rhodopseudomonas palustris strain CGA009 can synthesize three func...

journal_title：MicrobiologyOpen

authors： Heiniger EK,Harwood CS

更新日期：2015-08-01 00:00:00

abstract：:The plant growth-promoting rhizobacterium Pseudomonas fluorescens WCS374r produces several iron-regulated metabolites, including the fluorescent siderophore pseudobactin (Psb374), salicylic acid (SA), and pseudomonine (Psm), a siderophore that contains a SA moiety. After purification of Psb374 from culture supernatant...

journal_title：MicrobiologyOpen

authors： Djavaheri M,Mercado-Blanco J,Versluis C,Meyer JM,Loon LC,Bakker PA

更新日期：2012-09-01 00:00:00

abstract：:RodZ is important for maintaining the rod shape of Escherichia coli. Loss of RodZ causes conversion of the rod shape to a round shape and a growth rate slower than that of wild-type cells. Suppressor mutations that simultaneously restore both the growth rates and the rod shape were isolated. Most of the suppressor mut...

journal_title：MicrobiologyOpen

authors： Shiomi D,Niki H

更新日期：2013-10-01 00:00:00

abstract：:Probiotic bacteria are frequently used to treat intestinal diseases or to improve health; however, little is known about the evolutionary changes of these bacteria during probiotic manufacture and the bacterial ability to colonize the intestine. It has been observed that when bacteria adapt to a new environment, they ...

journal_title：MicrobiologyOpen

authors： López L,Calderón D,Cardenas P,Prado MB,Valle C,Trueba G

更新日期：2020-02-01 00:00:00

abstract：:Few studies have evaluated the trophic level in tropical caves, and none related the microbial biomass dynamics in the immobilization of carbon and nitrogen. Here, four tropical caves of Terra Ronca State Park, Brazil, were studied: Angélica, São Bernardo, Terra Ronca I, and Terra Ronca II caves. Physical, chemical, a...

journal_title：MicrobiologyOpen

authors： Paula CCP,Bichuette ME,Seleghim MHR

更新日期：2020-07-01 00:00:00

abstract：:Nuclease colicins bind their target receptor BtuB in the outer membrane of sensitive Escherichia coli cells in the form of a high-affinity complex with their cognate immunity proteins. The release of the immunity protein from the colicin complex is a prerequisite for cell entry of the colicin and occurs via a process ...

journal_title：MicrobiologyOpen

authors： Vankemmelbeke M,Housden NG,James R,Kleanthous C,Penfold CN

更新日期：2013-10-01 00:00:00

abstract：:Extracts from white birch have been reported to possess antimicrobial properties, but no study has linked the chemical composition of bark extract with antimicrobial activity. This study aimed to identify white birch (Betula papyrifera Marshall) bark extracts with antimicrobial activity and elucidate its composition. ...

journal_title：MicrobiologyOpen

authors： Blondeau D,St-Pierre A,Bourdeau N,Bley J,Lajeunesse A,Desgagné-Penix I

更新日期：2020-01-01 00:00:00

abstract：:The effect of elevated atmospheric CO2 concentration [CO2 ] on the diversity and composition of the prokaryotic community inhabiting the rhizosphere of winter barley (Hordeum vulgare L.) was investigated in a field experiment, using open-top chambers. Rhizosphere samples were collected at anthesis (flowering stage) fr...

journal_title：MicrobiologyOpen

authors： Szoboszlay M,Näther A,Mitterbauer E,Bender J,Weigel HJ,Tebbe CC

更新日期：2017-08-01 00:00:00

abstract：:A yeast expression plasmid was constructed containing a cardenolide biosynthetic module, referred to as CARD II, using the AssemblX toolkit, which enables the assembly of large DNA constructs. The genes cloned into the vector were (a) a Δ5 -3β-hydroxysteroid dehydrogenase gene from Digitalis lanata, (b) a steroid Δ5 -...

journal_title：MicrobiologyOpen

authors： Rieck C,Geiger D,Munkert J,Messerschmidt K,Petersen J,Strasser J,Meitinger N,Kreis W

更新日期：2019-12-01 00:00:00

abstract：:Citrus canker is an economically important disease that affects orange production in some of the most important producing areas around the world. It represents a great threat to the Brazilian and North American citriculture, particularly to the states of São Paulo and Florida, which together correspond to the biggest ...

journal_title：MicrobiologyOpen

authors： Sumares JA,Morão LG,Martins PM,Martins DA,Gomes E,Belasque J Jr,Ferreira H

更新日期：2016-04-01 00:00:00

abstract：:Resistance to colistin, mediated by chromosomal mutations and more recently, by plasmid-borne mcr genes, is increasingly being reported in bacterial isolates taken from humans, animals, farms, foods, and the environment. To easily identify and contain this quickly spreading menace, efficient diagnostics that are cheap...

journal_title：MicrobiologyOpen

authors： Osei Sekyere J

更新日期：2019-04-01 00:00:00

abstract：:Dinitrogen fixation, the biological reduction in N2 gas to ammonia contributes to the supply of new nitrogen in the surface ocean. To understand the diversity and abundance of potentially diazotrophic (N2 fixing) microorganisms associated with marine zooplankton, especially copepods, the nifH gene was studied using zo...

journal_title：MicrobiologyOpen

authors： Azimuddin KM,Hirai J,Suzuki S,Haider MN,Tachibana A,Watanabe K,Kitamura M,Hashihama F,Takahashi K,Hamasaki K

更新日期：2016-12-01 00:00:00

abstract：:Basic cosmetics was used by volunteers belonging to high (HHG) and low (LHG) hydration groups for 4 weeks, and bacterial communities and biophysical parameters in facial skin were analyzed. Hydration level increases and transepidermal water loss and roughness decreases were observed in both groups after cosmetic use. ...

journal_title：MicrobiologyOpen

authors： Lee HJ,Jeong SE,Lee S,Kim S,Han H,Jeon CO

更新日期：2018-04-01 00:00:00

abstract：:As part of the culturomics project aiming at describing the human microbiota, we report in this study the description of the new bacterial genus Raoultibacter gen. nov. that includes two new species, that is, R. massiliensis sp. nov. and R. timonensis sp. nov. The R. massiliensis type strain Marseille-P2849T was isola...

journal_title：MicrobiologyOpen

authors： Traore SI,Bilen M,Beye M,Diop A,Mbogning Fonkou MD,Tall ML,Michelle C,Yasir M,Ibraheem Azhar E,Bibi F,Bittar F,Jiman-Fatani AA,Daoud Z,Cadoret F,Fournier PE,Edouard S

更新日期：2019-06-01 00:00:00

abstract：:Biofilms play a central role in the pathobiology of otitis media (OM), bronchitis, sinusitis, conjunctivitis, and pneumonia caused by nontypeable Haemophilus influenzae (NTHI). Our previous studies show that extracellular DNA (eDNA) and DNABII proteins are essential components of biofilms formed by NTHI. The DNABII pr...

journal_title：MicrobiologyOpen

authors： Devaraj A,Buzzo J,Rocco CJ,Bakaletz LO,Goodman SD

更新日期：2018-06-01 00:00:00

abstract：:Microbial communities in sediments play an important role in alpine lake ecosystems. However, the microbial diversity and community composition of alpine lake sediments from the Hengduan Mountains remain largely unknown. Therefore, based on the Illumina MiSeq platform, high-throughput sequencing analysis of the 16S rR...

journal_title：MicrobiologyOpen

authors： Liao B,Yan X,Zhang J,Chen M,Li Y,Huang J,Lei M,He H,Wang J

更新日期：2019-09-01 00:00:00

abstract：:Sourdough fermentation is a traditional process that is used to improve bread quality. A spontaneous sourdough ecosystem consists of a mixture of flour and water that is fermented by endogenous lactic acid bacteria (LAB) and yeasts. The aim of this study was to identify bacterial diversity during backslopping of spont...

journal_title：MicrobiologyOpen

authors： Boreczek J,Litwinek D,Żylińska-Urban J,Izak D,Buksa K,Gawor J,Gromadka R,Bardowski JK,Kowalczyk M

更新日期：2020-04-01 00:00:00