Evaluation of two xenobiotic reductases from Pseudomonas putida for their suitability for magnetic nanoparticle-directed enzyme prodrug therapy as a novel approach to cancer treatment.

Abstract:

:Directed enzyme prodrug therapy (DEPT) is a cancer chemotherapy strategy in which bacterial enzymes are delivered to a cancer site before prodrug administration, resulting in prodrug activation at the cancer site and more localized treatment. A major limitation to DEPT is the poor effectiveness of the most studied enzyme for the CB1954 prodrug, NfnB from Escherichia coli, at concentrations suitable for human use. Much research into finding alternative enzymes to NfnB has resulted in the identification of the Xenobiotic reductases, XenA and XenB, which have been shown in the literature to reduce environmentally polluting nitro-compounds. In this study, they were assessed for their potential use in cancer prodrug therapy strategies. Both proteins were cloned into the pET28a+ expression vector to give the genetically modified proteins XenA-his and XenB-his, of which only XenB-his was active when tested with CB1954. XenB-his was further modified to include a cysteine-tag to facilitate direct immobilization on to a gold surface for future magnetic nanoparticle DEPT (MNDEPT) treatments and was named XenB-cys. When tested using high-performance liquid chromatography (HPLC), XenB-his and XenB-cys both demonstrated a preference for reducing CB1954 at the 4-nitro position. Furthermore, XenB-his and XenB-cys successfully induced cell death in SK-OV-3 cells when combined with CB1954. This led to XenB-cys being identified as a promising candidate for use in future MNDEPT treatments.

journal_name

Microbiologyopen

journal_title

MicrobiologyOpen

authors

Ball P,Halliwell J,Anderson S,Gwenin V,Gwenin C

doi

10.1002/mbo3.1110

subject

Has Abstract

pub_date

2020-10-01 00:00:00

pages

e1110

issue

10

issn

2045-8827

journal_volume

9

pub_type

杂志文章
  • Co-dynamics of Symbiodiniaceae and bacterial populations during the first year of symbiosis with Acropora tenuis juveniles.

    abstract::Interactions between corals and their associated microbial communities (Symbiodiniaceae and prokaryotes) are key to understanding corals' potential for and rate of acclimatory and adaptive responses. However, the establishment of microalgal and bacterial communities is poorly understood during coral ontogeny in the wi...

    journal_title:MicrobiologyOpen

    pub_type: 杂志文章

    doi:10.1002/mbo3.959

    authors: Quigley KM,Alvarez Roa C,Torda G,Bourne DG,Willis BL

    更新日期:2020-02-01 00:00:00

  • Phage therapy treatment of the coral pathogen Vibrio coralliilyticus.

    abstract::Vibrio coralliilyticus is an important coral pathogen demonstrated to cause disease outbreaks worldwide. This study investigated the feasibility of applying bacteriophage therapy to treat the coral pathogen V. coralliilyticus. A specific bacteriophage for V. coralliilyticus strain P1 (LMG23696), referred to here as ba...

    journal_title:MicrobiologyOpen

    pub_type: 杂志文章

    doi:10.1002/mbo3.52

    authors: Cohen Y,Joseph Pollock F,Rosenberg E,Bourne DG

    更新日期:2013-02-01 00:00:00

  • CopM is a novel copper-binding protein involved in copper resistance in Synechocystis sp. PCC 6803.

    abstract::Copper resistance system in the cyanobacterium Synechocystis sp. PCC 6803 comprises two operons, copMRS and copBAC, which are expressed in response to copper in the media. copBAC codes for a heavy-metal efflux-resistance nodulation and division (HME-RND) system, while copMRS codes for a protein of unknown function, Co...

    journal_title:MicrobiologyOpen

    pub_type: 杂志文章

    doi:10.1002/mbo3.231

    authors: Giner-Lamia J,López-Maury L,Florencio FJ

    更新日期:2015-02-01 00:00:00

  • Immunogenicity of a bivalent protein as a vaccine against Edwardsiella anguillarum and Vibrio vulnificus in Japanese eel (Anguilla japonica).

    abstract::The OMPs A (OmpA)-of Edwardsiella anguillarum and OmpU of V. vulnificus have been proven to be good antigens. In this study, after construction of a vector, a new recombinant Omp (rOMP) containing both OmpA and OmpU was expressed and purified. Then, the Japanese eels (Anguilla japonica) were intraperitoneally (i.p.) i...

    journal_title:MicrobiologyOpen

    pub_type: 杂志文章

    doi:10.1002/mbo3.766

    authors: Guo S,Hu L,Feng J,Lin P,He L,Yan Q

    更新日期:2019-06-01 00:00:00

  • Biosynthetic approach to combine the first steps of cardenolide formation in Saccharomyces cerevisiae.

    abstract::A yeast expression plasmid was constructed containing a cardenolide biosynthetic module, referred to as CARD II, using the AssemblX toolkit, which enables the assembly of large DNA constructs. The genes cloned into the vector were (a) a Δ5 -3β-hydroxysteroid dehydrogenase gene from Digitalis lanata, (b) a steroid Δ5 -...

    journal_title:MicrobiologyOpen

    pub_type: 杂志文章

    doi:10.1002/mbo3.925

    authors: Rieck C,Geiger D,Munkert J,Messerschmidt K,Petersen J,Strasser J,Meitinger N,Kreis W

    更新日期:2019-12-01 00:00:00

  • A preliminary examination of the bacterial, archaeal, and fungal rhizosphere microbiome in healthy and Phellinus noxius-infected trees.

    abstract::Phellinus noxius is a pathogenic fungus that causes brown root rot disease, resulting in a widespread tree and crop mortality in the tropics and subtropics. Early stages of this disease are largely asymptomatic, hindering early diagnosis and effective treatment. We hypothesized that P. noxius infection would alter the...

    journal_title:MicrobiologyOpen

    pub_type: 杂志文章

    doi:10.1002/mbo3.1115

    authors: Tsang KSW,Cheung MK,Lam RYC,Kwan HS

    更新日期:2020-10-01 00:00:00

  • Noncontiguous finished genome sequence and description of Raoultibacter massiliensis gen. nov., sp. nov. and Raoultibacter timonensis sp. nov, two new bacterial species isolated from the human gut.

    abstract::As part of the culturomics project aiming at describing the human microbiota, we report in this study the description of the new bacterial genus Raoultibacter gen. nov. that includes two new species, that is, R. massiliensis sp. nov. and R. timonensis sp. nov. The R. massiliensis type strain Marseille-P2849T was isola...

    journal_title:MicrobiologyOpen

    pub_type: 杂志文章

    doi:10.1002/mbo3.758

    authors: Traore SI,Bilen M,Beye M,Diop A,Mbogning Fonkou MD,Tall ML,Michelle C,Yasir M,Ibraheem Azhar E,Bibi F,Bittar F,Jiman-Fatani AA,Daoud Z,Cadoret F,Fournier PE,Edouard S

    更新日期:2019-06-01 00:00:00

  • Autoinducer2 affects trimethoprim-sulfamethoxazole susceptibility in avian pathogenic Escherichia coli dependent on the folate synthesis-associate pathway.

    abstract::Avian pathogenic Escherichia coli (APEC) causes airsacculitis, polyserositis, septicemia, and other mainly extraintestinal diseases in chickens, ducks, geese, pigeons, and other avian species, and is responsible for great economic losses in the avian industry. The autoinducer 2 (AI-2) quorum sensing system is widely p...

    journal_title:MicrobiologyOpen

    pub_type: 杂志文章

    doi:10.1002/mbo3.582

    authors: Yu L,Li W,Zhang M,Cui Y,Chen X,Ni J,Yu L,Shang F,Xue T

    更新日期:2018-08-01 00:00:00

  • Selective chemoattraction of the benthic diatom Seminavis robusta to phosphate but not to inorganic nitrogen sources contributes to biofilm structuring.

    abstract::Diatoms frequently dominate marine and freshwater biofilms as major primary producers. Nutrient resources in these biofilms are patchily distributed and fluctuate dynamically over time. We recently reported that this spatially and temporally structured environment can be exploited by motile diatoms that use chemoattra...

    journal_title:MicrobiologyOpen

    pub_type: 杂志文章

    doi:10.1002/mbo3.694

    authors: V Bondoc KG,Lembke C,Vyverman W,Pohnert G

    更新日期:2019-04-01 00:00:00

  • XatA, an AT-1 autotransporter important for the virulence of Xylella fastidiosa Temecula1.

    abstract::Xylella fastidiosa Temecula1 is the causative agent of Pierce's disease of grapevine, which is spread by xylem-feeding insects. An important feature of the infection cycle is the ability of X. fastidiosa to colonize and interact with two distinct environments, the xylem of susceptible plants and the insect foregut. He...

    journal_title:MicrobiologyOpen

    pub_type: 杂志文章

    doi:10.1002/mbo3.6

    authors: Matsumoto A,Huston SL,Killiny N,Igo MM

    更新日期:2012-03-01 00:00:00

  • Temporal transcriptomes of a marine cyanopodovirus and its Synechococcus host during infection.

    abstract::Marine picocyanobacteria belonging to genera Synechococcus and Prochlorococcus are genetically diverged and distributed into distinct biogeographical patterns, and both are infected by genetically closely related cyanopodoviruses. Previous studies have not fully explored whether the two virus-host systems share simila...

    journal_title:MicrobiologyOpen

    pub_type: 杂志文章

    doi:10.1002/mbo3.1150

    authors: Huang S,Sun Y,Zhang S,Long L

    更新日期:2020-12-30 00:00:00

  • Hexyl gallate for the control of citrus canker caused by Xanthomonas citri subsp citri.

    abstract::Brazil is the biggest producer of sweet oranges and the main exporter of concentrated orange juice in the world. Among the diseases that affect citriculture, Asiatic citrus canker, caused by the bacterial pathogen Xanthomonas citri, represents one of the most significant threats. The current Brazilian legislation regu...

    journal_title:MicrobiologyOpen

    pub_type: 杂志文章

    doi:10.1002/mbo3.1104

    authors: Cavalca LB,Zamuner CFC,Saldanha LL,Polaquini CR,Regasini LO,Behlau F,Ferreira H

    更新日期:2020-09-01 00:00:00

  • Taxonomic and functional profiles of soil samples from Atlantic forest and Caatinga biomes in northeastern Brazil.

    abstract::Although microorganisms play crucial roles in ecosystems, metagenomic analyses of soil samples are quite scarce, especially in the Southern Hemisphere. In this work, the microbial diversity of soil samples from an Atlantic Forest and Caatinga was analyzed using a metagenomic approach. Proteobacteria and Actinobacteria...

    journal_title:MicrobiologyOpen

    pub_type: 杂志文章

    doi:10.1002/mbo3.169

    authors: Pacchioni RG,Carvalho FM,Thompson CE,Faustino AL,Nicolini F,Pereira TS,Silva RC,Cantão ME,Gerber A,Vasconcelos AT,Agnez-Lima LF

    更新日期:2014-06-01 00:00:00

  • Oscillating behavior of Clostridium difficile Min proteins in Bacillus subtilis.

    abstract::In rod-shaped bacteria, the proper placement of the division septum at the midcell relies, at least partially, on the proteins of the Min system as an inhibitor of cell division. The main principle of Min system function involves the formation of an inhibitor gradient along the cell axis; however, the establishment of...

    journal_title:MicrobiologyOpen

    pub_type: 杂志文章

    doi:10.1002/mbo3.337

    authors: Makroczyová J,Jamroškovič J,Krascsenitsová E,Labajová N,Barák I

    更新日期:2016-06-01 00:00:00

  • Comparative genomics of hepatitis A virus, hepatitis C virus, and hepatitis E virus provides insights into the evolutionary history of Hepatovirus species.

    abstract::The intraspecies genomic diversity of the single-strand RNA (+) virus species hepatitis A virus (Hepatovirus), hepatitis C virus (Hepacivirus), and hepatitis E virus (Orthohepevirus) was compared. These viral species all can cause liver inflammation (hepatitis), but share no gene similarity. The codon usage of human h...

    journal_title:MicrobiologyOpen

    pub_type: 杂志文章

    doi:10.1002/mbo3.973

    authors: Wassenaar TM,Jun SR,Robeson M,Ussery DW

    更新日期:2020-02-01 00:00:00

  • Characterizing the bacterial community across the gastrointestinal tract of goats: Composition and potential function.

    abstract::The composition and function of the microbial community in the gastrointestinal tract (GIT) have increasingly captured the attention of nutritionists because these traits affect the nutrient utilization efficiency and health of host animals. Little information has been reported on these aspects of the goat GIT. This s...

    journal_title:MicrobiologyOpen

    pub_type: 杂志文章

    doi:10.1002/mbo3.820

    authors: Wang L,Jin L,Xue B,Wang Z,Peng Q

    更新日期:2019-09-01 00:00:00

  • The DNABII family of proteins is comprised of the only nucleoid associated proteins required for nontypeable Haemophilus influenzae biofilm structure.

    abstract::Biofilms play a central role in the pathobiology of otitis media (OM), bronchitis, sinusitis, conjunctivitis, and pneumonia caused by nontypeable Haemophilus influenzae (NTHI). Our previous studies show that extracellular DNA (eDNA) and DNABII proteins are essential components of biofilms formed by NTHI. The DNABII pr...

    journal_title:MicrobiologyOpen

    pub_type: 杂志文章

    doi:10.1002/mbo3.563

    authors: Devaraj A,Buzzo J,Rocco CJ,Bakaletz LO,Goodman SD

    更新日期:2018-06-01 00:00:00

  • Glycosylated amyloid-like proteins in the structural extracellular polymers of aerobic granular sludge enriched with ammonium-oxidizing bacteria.

    abstract::A new type of structural extracellular polymers (EPS) was extracted from aerobic granular sludge dominated by ammonium-oxidizing bacteria. It was analyzed by Raman and FTIR spectroscopy to characterize specific amino acids and protein secondary structure, and by SDS-PAGE with different stains to identify different gly...

    journal_title:MicrobiologyOpen

    pub_type: 杂志文章

    doi:10.1002/mbo3.616

    authors: Lin Y,Reino C,Carrera J,Pérez J,van Loosdrecht MCM

    更新日期:2018-12-01 00:00:00

  • The effect of sulfamonomethoxine treatment on the gut microbiota of Nile tilapia (Oreochromis niloticus).

    abstract::To investigate the possible effects of sulfamonomethoxine (SMM) on Nile tilapia (Oreochromis niloticus), we quantitatively evaluated the microbial shifts in the intestines of Nile tilapia in response to different doses of SMM (200 and 300 mg/kg) using 16S rRNA gene sequencing. At the phylum level, the control group (0...

    journal_title:MicrobiologyOpen

    pub_type: 杂志文章

    doi:10.1002/mbo3.1116

    authors: Ming J,Fu Z,Ma Z,Zhou L,Zhang Z,Song C,Yuan X,Wu Q

    更新日期:2020-11-01 00:00:00

  • Mapping and determinism of soil microbial community distribution across an agricultural landscape.

    abstract::Despite the relevance of landscape, regarding the spatial patterning of microbial communities and the relative influence of environmental parameters versus human activities, few investigations have been conducted at this scale. Here, we used a systematic grid to characterize the distribution of soil microbial communit...

    journal_title:MicrobiologyOpen

    pub_type: 杂志文章

    doi:10.1002/mbo3.255

    authors: Constancias F,Terrat S,Saby NP,Horrigue W,Villerd J,Guillemin JP,Biju-Duval L,Nowak V,Dequiedt S,Ranjard L,Chemidlin Prévost-Bouré N

    更新日期:2015-06-01 00:00:00

  • Immunity protein release from a cell-bound nuclease colicin complex requires global conformational rearrangement.

    abstract::Nuclease colicins bind their target receptor BtuB in the outer membrane of sensitive Escherichia coli cells in the form of a high-affinity complex with their cognate immunity proteins. The release of the immunity protein from the colicin complex is a prerequisite for cell entry of the colicin and occurs via a process ...

    journal_title:MicrobiologyOpen

    pub_type: 杂志文章

    doi:10.1002/mbo3.122

    authors: Vankemmelbeke M,Housden NG,James R,Kleanthous C,Penfold CN

    更新日期:2013-10-01 00:00:00

  • Shared and unique microbes between Small hive beetles (Aethina tumida) and their honey bee hosts.

    abstract::The small hive beetle (SHB) is an opportunistic parasite that feeds on bee larvae, honey, and pollen. While SHBs can also feed on fruit and other plant products, like its plant-feeding relatives, SHBs prefer to feed on hive resources and only reproduce inside bee colonies. As parasites, SHBs are inevitably exposed to ...

    journal_title:MicrobiologyOpen

    pub_type: 杂志文章

    doi:10.1002/mbo3.899

    authors: Huang Q,Lopez D,Evans JD

    更新日期:2019-10-01 00:00:00

  • Biological degradation of aflatoxin M1 by Bacillus pumilus E-1-1-1.

    abstract::Aflatoxin M1 (AFM1 ) is a potent mycotoxin which causes serious health concerns in developing countries, where it is mainly found in milk, meat, and other foods. Biological detoxification is a promising method for eliminating AFM1 . The aim of this work was to search for AFM1 -degrading bacterial strains from animal w...

    journal_title:MicrobiologyOpen

    pub_type: 杂志文章

    doi:10.1002/mbo3.663

    authors: Gu X,Sun J,Cui Y,Wang X,Sang Y

    更新日期:2019-03-01 00:00:00

  • Spatial patterns in soil physicochemical and microbiological properties in a grassland adjacent to a newly built lake.

    abstract::Soil water content (SWC) is an important determinant for nutrient cycling and microorganism activity in the grassland ecosystem. Lakes have a positive effect on the water supply of the neighboring ecosystem. However, information evaluating whether newly built lakes improve the physiochemical properties and microorgani...

    journal_title:MicrobiologyOpen

    pub_type: 杂志文章

    doi:10.1002/mbo3.912

    authors: Li J,An C,Shang J,Zhao T,Zhang Q,Yang X,Ren C,Huang D,Liu K,Shao X

    更新日期:2019-12-01 00:00:00

  • Global transcriptome responses including small RNAs during mixed-species interactions with methicillin-resistant Staphylococcus aureus and Pseudomonas aeruginosa.

    abstract::Pseudomonas aeruginosa and Staphylococcus aureus mixed-species biofilm infections are more resilient to biocide attacks compared to their single-species counterparts. Therefore, this study used an in vitro model recapitulating bacterial burdens seen in in vivo infections to investigate the interactions of P. aeruginos...

    journal_title:MicrobiologyOpen

    pub_type: 杂志文章

    doi:10.1002/mbo3.427

    authors: Miller CL,Van Laar TA,Chen T,Karna SLR,Chen P,You T,Leung KP

    更新日期:2017-06-01 00:00:00

  • Mcr colistin resistance gene: a systematic review of current diagnostics and detection methods.

    abstract::Resistance to colistin, mediated by chromosomal mutations and more recently, by plasmid-borne mcr genes, is increasingly being reported in bacterial isolates taken from humans, animals, farms, foods, and the environment. To easily identify and contain this quickly spreading menace, efficient diagnostics that are cheap...

    journal_title:MicrobiologyOpen

    pub_type: 杂志文章

    doi:10.1002/mbo3.682

    authors: Osei Sekyere J

    更新日期:2019-04-01 00:00:00

  • Pressure and temperature effects on deep-sea hydrocarbon-degrading microbial communities in subarctic sediments.

    abstract::The Hatton-Rockall Basin (North-East Atlantic) is an area with potential for deep-sea (2,900 m) hydrocarbon exploration. Following the Deepwater Horizon oil spill, many investigations into the responses of sediment microbial communities to oil pollution have been undertaken. However, hydrostatic pressure is a paramete...

    journal_title:MicrobiologyOpen

    pub_type: 杂志文章

    doi:10.1002/mbo3.768

    authors: Perez Calderon LJ,Gontikaki E,Potts LD,Shaw S,Gallego A,Anderson JA,Witte U

    更新日期:2019-06-01 00:00:00

  • UV Resistance of bacteria from the Kenyan Marine cyanobacterium Moorea producens.

    abstract::UV resistance of bacteria isolated from the marine cyanobacterium Moorea producens has not been observed previously, findings which highlight how unsafe germicidal UV irradiation for sterilization of air, food, and water could be. Further, UV resistance of Bacillus licheniformis is being observed for the first time. T...

    journal_title:MicrobiologyOpen

    pub_type: 杂志文章

    doi:10.1002/mbo3.697

    authors: Dzeha T,Nyiro C,Kardasopoulos D,Mburu D,Mwafaida J,Hall MJ,Burgess JG

    更新日期:2019-04-01 00:00:00

  • The effects of low levels of trivalent ions on a standard strain of Escherichia coli (ATCC 11775) in aqueous solutions.

    abstract::Considering the ever-growing usage of trivalent salts in water treatment, for example, lanthanum salts in rare earth, AlCl3 and FeCl3 , the effects of different trivalent cations on the bacterium Escherichia coli (E. coli) ATCC 11775 strain have been studied in aqueous solutions. From colony incubation studies, the co...

    journal_title:MicrobiologyOpen

    pub_type: 杂志文章

    doi:10.1002/mbo3.574

    authors: Deng C,Li X,Xue X,Pashley RM

    更新日期:2018-06-01 00:00:00

  • Assessment of carbapenem-resistant Enterobacteriaceae-plate formula and quality control procedure.

    abstract:AIMS:To assess a cost-effective in-house selective plate formula for actively screening carbapenem-resistant Enterobacteriaceae (CRE). METHODOLOGY AND RESULTS:The in-house formula included CHROMagarTM Orientation, meropenem, and ingredients present in the Mac-Conkey formula, such as bile salts and crystal violet (pH 6...

    journal_title:MicrobiologyOpen

    pub_type: 杂志文章

    doi:10.1002/mbo3.1130

    authors: Tran TC,Pham BT,Pham VH,Ngo TA,Hanberger H,Larsson M,Olson L

    更新日期:2020-12-01 00:00:00