当前位置： SCI文献检索 > Scientific Reports期刊下所有文献 > Direct visualization of the extracellular binding structure of E-cadherins in liquid.

Direct visualization of the extracellular binding structure of E-cadherins in liquid.

Abstract：

:E-cadherin is a key Ca-dependent cell adhesion molecule, which is expressed on many cell surfaces and involved in cell morphogenesis, embryonic development, EMT, etc. The fusion protein E-cad-Fc consists of the extracellular domain of E-cadherin and the IgG Fc domain. On plates coated with this chimeric protein, ES/iPS cells are cultivated particularly well and induced to differentiate. The cells adhere to the plate via E-cad-Fc in the presence of Ca2+ and detach by a chelating agent. For the purpose of clarifying the structures of E-cad-Fc in the presence and absence of Ca2+, we analyzed the molecular structure of E-cad-Fc by AFM in liquid. Our AFM observations revealed a rod-like structure of the entire extracellular domain of E-cad-Fc in the presence of Ca2+ as well as trans-binding of E-cad-Fc with adjacent molecules, which may be the first, direct confirmation of trans-dimerization of E-cadherin. The observed structures were in good agreement with an X-ray crystallographic model. Furthermore, we succeeded in visualizing the changes in the rod-like structure of the EC domains with and without calcium. The biomatrix surface plays an important role in cell culture, so the analysis of its structure and function may help promote cell engineering based on cell recognition.

journal_name

Sci Rep

journal_title

Scientific reports

authors

Shibata-Seki T,Nagaoka M,Goto M,Kobatake E,Akaike T

doi

10.1038/s41598-020-72517-2

subject

Has Abstract

pub_date

2020-10-12 00:00:00

pages

17044

issue

1

issn

2045-2322

pii

10.1038/s41598-020-72517-2

journal_volume

10

pub_type

杂志文章

相关文献

Scientific Reports文献大全
  • Mathematical modeling of the cardiovascular autonomic control in healthy subjects during a passive head-up tilt test.

    abstract：:A mathematical model is proposed for the autonomic control of cardiovascular system, which takes into account two separated self-exciting sympathetic control loops of heart rate and peripheral vascular tone. The control loops are represented by self-exciting time-delay systems and their tone depends on activity of the...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-020-71532-7

    authors： Ishbulatov YM,Karavaev AS,Kiselev AR,Simonyan MA,Prokhorov MD,Ponomarenko VI,Mironov SA,Gridnev VI,Bezruchko BP,Shvartz VA

    更新日期：2020-10-05 00:00:00

  • Revealing Tumor Habitats from Texture Heterogeneity Analysis for Classification of Lung Cancer Malignancy and Aggressiveness.

    abstract：:We propose an approach for characterizing structural heterogeneity of lung cancer nodules using Computed Tomography Texture Analysis (CTTA). Measures of heterogeneity were used to test the hypothesis that heterogeneity can be used as predictor of nodule malignancy and patient survival. To do this, we use the National ...

    journal_title：Scientific reports

    pub_type： 杂志文章,随机对照试验

    doi：10.1038/s41598-019-38831-0

    authors： Cherezov D,Goldgof D,Hall L,Gillies R,Schabath M,Müller H,Depeursinge A

    更新日期：2019-03-14 00:00:00

  • Comprehensive profiling of JMJD3 in gastric cancer and its influence on patient survival.

    abstract：:Histone methylation is thought to control the regulation of genetic program and the dysregulation of it has been found to be closely associated with cancer. JMJD3 has been identified as an H3K27 demethylase and its role in cancer development is context specific. The role of JMJD3 in gastric cancer (GC) has not been ex...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-018-37340-w

    authors： Xu Z,Xia Y,Xiao Z,Jia Y,Li L,Jin Y,Zhao Q,Wan L,Yi T,Yu Y,Wen Q,Zhu Y,Qin B,Zhang F,Shen J

    更新日期：2019-01-29 00:00:00

  • Two-dimensional echocardiography after return of spontaneous circulation and its association with in-hospital survival after in-hospital cardiopulmonary resuscitation.

    abstract：:This retrospective cohort study investigated the association between in-hospital survival and two-dimensional (2D) echocardiography within 24 hours after the return of spontaneous circulation (ROSC) in patients who underwent in-hospital cardiopulmonary resuscitation (ICPR) after in-hospital cardiopulmonary arrest (IHC...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-019-56153-z

    authors： Song IA,Cha JK,Oh TK,Jo YH,Yoon YE

    更新日期：2020-01-08 00:00:00

  • Ca(2+) dynamics in oocytes from naturally-aged mice.

    abstract：:The ability of human metaphase-II arrested eggs to activate following fertilisation declines with advancing maternal age. Egg activation is triggered by repetitive increases in intracellular Ca(2+) concentration ([Ca(2+)]i) in the ooplasm as a result of sperm-egg fusion. We therefore hypothesised that eggs from older ...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep19357

    authors： Haverfield J,Nakagawa S,Love D,Tsichlaki E,Nomikos M,Lai FA,Swann K,FitzHarris G

    更新日期：2016-01-20 00:00:00

  • Mind wandering simultaneously prolongs reactions and promotes creative incubation.

    abstract：:Mind wandering (MW) refers to the disengagement of attention from the external environment and the generation of thoughts unrelated to the task at hand. It is a ubiquitous cognitive process resulting in lapses of attention. MW imposes a negative impact on attention-based task performance, but also has been associated ...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-017-10616-3

    authors： Leszczynski M,Chaieb L,Reber TP,Derner M,Axmacher N,Fell J

    更新日期：2017-08-31 00:00:00

  • Structure and co-occurrence patterns in microbial communities under acute environmental stress reveal ecological factors fostering resilience.

    abstract：:Understanding the factors that modulate bacterial community assembly in natural soils is a longstanding challenge in microbial community ecology. In this work, we compared two microbial co-occurrence networks representing bacterial soil communities from two different sections of a pH, temperature and humidity gradient...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-018-23931-0

    authors： Mandakovic D,Rojas C,Maldonado J,Latorre M,Travisany D,Delage E,Bihouée A,Jean G,Díaz FP,Fernández-Gómez B,Cabrera P,Gaete A,Latorre C,Gutiérrez RA,Maass A,Cambiazo V,Navarrete SA,Eveillard D,González M

    更新日期：2018-04-12 00:00:00

  • High dietary salt intake correlates with modulated Th17-Treg cell balance resulting in enhanced bone loss and impaired bone-microarchitecture in male mice.

    abstract：:Osteoporosis is associated with reduced density and quality of bone leading to weakened skeleton thereby increasing the risk of fractures responsible for increased morbidity and mortality. Due to preference for western food style the consumption of salt intake in our diets has increased many folds. High dietary salt i...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-018-20896-y

    authors： Dar HY,Singh A,Shukla P,Anupam R,Mondal RK,Mishra PK,Srivastava RK

    更新日期：2018-02-06 00:00:00

  • Localized Down-regulation of P-glycoprotein by Focused Ultrasound and Microbubbles induced Blood-Brain Barrier Disruption in Rat Brain.

    abstract：:Multi-drug resistant efflux transporters found in Blood-Brain Barrier (BBB) acts as a functional barrier, by pumping out most of the drugs into the blood. Previous studies showed focused ultrasound (FUS) induced microbubble oscillation can disrupt the BBB by loosening the tight junctions in the brain endothelial cells...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep31201

    authors： Cho H,Lee HY,Han M,Choi JR,Ahn S,Lee T,Chang Y,Park J

    更新日期：2016-08-11 00:00:00

  • Genetic aetiology of self-harm ideation and behaviour.

    abstract：:Family studies have identified a heritable component to self-harm that is partially independent from comorbid psychiatric disorders. However, the genetic aetiology of broad sense (non-suicidal and suicidal) self-harm has not been characterised on the molecular level. In addition, controversy exists about the degree to...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-020-66737-9

    authors： Campos AI,Verweij KJH,Statham DJ,Madden PAF,Maciejewski DF,Davis KAS,John A,Hotopf M,Heath AC,Martin NG,Rentería ME

    更新日期：2020-06-16 00:00:00

  • Author Correction: High-throughput format for the phenotyping of fungi on solid substrates.

    abstract：:An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...

    journal_title：Scientific reports

    pub_type： 已发布勘误

    doi：10.1038/s41598-020-61396-2

    authors： Cánovas D,Studt L,Marcos AT,Strauss J

    更新日期：2020-03-06 00:00:00

  • Perfluorooctanoic acid in indoor particulate matter triggers oxidative stress and inflammation in corneal and retinal cells.

    abstract：:To investigate the particle size distribution of particulate matter and the concentration of specific perfluorinated compounds in indoor dust samples from several locations. Then, we used cell-based assays to investigate the effect of perfluorinated compounds on human corneal epithelial (HCEpiC), endothelial cells (HC...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-020-72600-8

    authors： Tien PT,Lin HJ,Tsai YY,Lim YP,Chen CS,Chang CY,Lin CJ,Chen JJ,Wu SM,Huang YJ,Wan L

    更新日期：2020-09-24 00:00:00

  • A versatile clearing agent for multi-modal brain imaging.

    abstract：:Extensive mapping of neuronal connections in the central nervous system requires high-throughput µm-scale imaging of large volumes. In recent years, different approaches have been developed to overcome the limitations due to tissue light scattering. These methods are generally developed to improve the performance of a...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep09808

    authors： Costantini I,Ghobril JP,Di Giovanna AP,Allegra Mascaro AL,Silvestri L,Müllenbroich MC,Onofri L,Conti V,Vanzi F,Sacconi L,Guerrini R,Markram H,Iannello G,Pavone FS

    更新日期：2015-05-07 00:00:00

  • A humanized mouse model identifies key amino acids for low immunogenicity of H7N9 vaccines.

    abstract：:Influenza vaccines of H7N9 subtype are consistently less immunogenic in humans than vaccines developed for other subtypes. Although prior immunoinformatic analysis identified T-cell epitopes in H7 hemagglutinin (HA) which potentially enhance regulatory T cell response due to conservation with the human genome, the lin...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-017-01372-5

    authors： Wada Y,Nithichanon A,Nobusawa E,Moise L,Martin WD,Yamamoto N,Terahara K,Hagiwara H,Odagiri T,Tashiro M,Lertmemongkolchai G,Takeyama H,De Groot AS,Ato M,Takahashi Y

    更新日期：2017-04-28 00:00:00

  • Yield-phenology relations and water use efficiency of maize (Zea mays L.) in ridge-furrow mulching system in semiarid east African Plateau.

    abstract：:Yield-phenology relation is a critical issue affecting rainfed maize field productivity in semiarid east African Plateau (EAP). We first introduced Chinese ridge-furrow mulching (RFM) system to EAP, using three maize cultivars with early-, mid- and late-maturing traits as test materials. A two-year field experiment wa...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-017-03372-x

    authors： Mo F,Wang JY,Li FM,Nguluu SN,Ren HX,Zhou H,Zhang J,Kariuki CW,Gicheru P,Kavagi L,Cheruiyot WK,Xiong YC

    更新日期：2017-06-12 00:00:00

  • Electricity from lignocellulosic substrates by thermophilic Geobacillus species.

    abstract：:Given our vast lignocellulosic biomass reserves and the difficulty in bioprocessing them without expensive pretreatment and fuel separation steps, the conversion of lignocellulosic biomass directly into electricity would be beneficial. Here we report the previously unexplored capabilities of thermophilic Geobacillus s...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-020-72866-y

    authors： Shrestha N,Tripathi AK,Govil T,Sani RK,Urgun-Demirtas M,Kasthuri V,Gadhamshetty V

    更新日期：2020-10-12 00:00:00

  • Modulation of light-driven arousal by LIM-homeodomain transcription factor Apterous in large PDF-positive lateral neurons of the Drosophila brain.

    abstract：:Apterous (Ap), the best studied LIM-homeodomain transcription factor in Drosophila, cooperates with the cofactor Chip (Chi) to regulate transcription of specific target genes. Although Ap regulates various developmental processes, its function in the adult brain remains unclear. Here, we report that Ap and Chi in the ...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep37255

    authors： Shimada N,Inami S,Sato S,Kitamoto T,Sakai T

    更新日期：2016-11-17 00:00:00

  • Pattern of retinal morphological and functional decay in a light-inducible, rhodopsin mutant mouse.

    abstract：:Hallmarks of Retinitis Pigmentosa (RP), a family of genetic diseases, are a typical rod-cone-degeneration with initial night blindness and loss of peripheral vision, followed by decreased daylight sight and progressive visual acuity loss up to legal blindness. Great heterogeneity in nature and function of mutated gene...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-017-06045-x

    authors： Gargini C,Novelli E,Piano I,Biagioni M,Strettoi E

    更新日期：2017-07-18 00:00:00

  • Ultralow power spin-orbit torque magnetization switching induced by a non-epitaxial topological insulator on Si substrates.

    abstract：:The large spin Hall effect in topological insulators (TIs) is very attractive for ultralow-power spintronic devices. However, evaluation of the spin Hall angle and spin-orbit torque (SOT) of TIs is usually performed on high-quality single-crystalline TI thin films grown on dedicated III-V semiconductor substrates. Her...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-020-69027-6

    authors： Khang NHD,Nakano S,Shirokura T,Miyamoto Y,Hai PN

    更新日期：2020-07-22 00:00:00

  • Muscle metabolic remodelling patterns in Duchenne muscular dystrophy revealed by ultra-high-resolution mass spectrometry imaging.

    abstract：:Duchenne muscular dystrophy (DMD) is a common and severe X-linked myopathy, characterized by muscle degeneration due to altered or absent dystrophin. DMD has no effective cure, and the underlying molecular mechanisms remain incompletely understood. The aim of this study is to investigate the metabolic changes in DMD u...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-021-81090-1

    authors： Dabaj I,Ferey J,Marguet F,Gilard V,Basset C,Bahri Y,Brehin AC,Vanhulle C,Leturcq F,Marret S,Laquerrière A,Schmitz-Afonso I,Afonso C,Bekri S,Tebani A

    更新日期：2021-01-21 00:00:00

  • Systemic treatment of patients with metachronous peritoneal carcinomatosis of colorectal origin.

    abstract：:Combining chemotherapy and targeted therapies has resulted in an enhanced survival in metastatic colorectal cancer (mCRC) patients. However, the result of this palliative treatment in patients with metachronous peritoneal carcinomatosis (PC) remains unknown. The current population-based study aims to investigate the u...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep18632

    authors： van Oudheusden TR,Razenberg LG,van Gestel YR,Creemers GJ,Lemmens VE,de Hingh IH

    更新日期：2015-12-21 00:00:00

  • Tailored plasmon-induced transparency in attenuated total reflection response in a metal-insulator-metal structure.

    abstract：:We demonstrated tailored plasmon-induced transparency (PIT) in a metal (Au)-insulator (SiO2)-metal (Ag) (MIM) structure, where the Fano interference between the MIM waveguide mode and the surface plasmon polariton (SPP) resonance mode induced a transparency window in an otherwise opaque wavenumber (k) region. A series...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-017-17847-4

    authors： Matsunaga K,Hirai Y,Neo Y,Matsumoto T,Tomita M

    更新日期：2017-12-19 00:00:00

  • A new high-resolution melting analysis for the detection and identification of Plasmodium in human and Anopheles vectors of malaria.

    abstract：:Among vector-borne diseases malaria is the leading cause of morbidity in the world, with more than 200 million cases per year and a large number of deaths. The techniques traditionally used for the detection of Plasmodium in humans and Anopheles mosquitoes include microscopy, IRMA, ELISA, antibody or molecular assays,...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-018-36515-9

    authors： Murillo E,Muskus C,Agudelo LA,Vélez ID,Ruiz-Lopez F

    更新日期：2019-02-08 00:00:00

  • Shape Tailored Magnetic Nanorings for Intracellular Hyperthermia Cancer Therapy.

    abstract：ABSTARCT:This work explores a new class of vortex/magnetite/iron oxide nanoparticles designed for magnetic hyperthermia applications. These nanoparticles, named Vortex Iron oxide Particles (VIPs), are an alternative to the traditional Superparamagnetic Iron Oxide Nanoparticles (SPIONs), since VIPs present superior heat...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-017-14633-0

    authors： Dias CSB,Hanchuk TDM,Wender H,Shigeyosi WT,Kobarg J,Rossi AL,Tanaka MN,Cardoso MB,Garcia F

    更新日期：2017-11-01 00:00:00

  • Perception of Discrete Emotions in Others: Evidence for Distinct Facial Mimicry Patterns.

    abstract：:Covert facial mimicry involves subtle facial muscle activation in observers when they perceive the facial emotional expressions of others. It remains uncertain whether prototypical facial features in emotional expressions are being covertly mimicked and also whether covert facial mimicry involves distinct facial muscl...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-020-61563-5

    authors： Wingenbach TSH,Brosnan M,Pfaltz MC,Peyk P,Ashwin C

    更新日期：2020-03-13 00:00:00

  • Vagus Nerve Stimulation Improves Cardiac Function by Preventing Mitochondrial Dysfunction in Obese-Insulin Resistant Rats.

    abstract：:Long-term high-fat diet (HFD) consumption leads to not only obese-insulin resistance, but also impaired left ventricular (LV) function. Vagus nerve stimulation (VNS) has been shown to exert cardioprotection. However, its effects on the heart and metabolic parameters under obese-insulin resistant condition is not known...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep19749

    authors： Samniang B,Shinlapawittayatorn K,Chunchai T,Pongkan W,Kumfu S,Chattipakorn SC,KenKnight BH,Chattipakorn N

    更新日期：2016-02-01 00:00:00

  • A New Strategy for Analyzing Time-Series Data Using Dynamic Networks: Identifying Prospective Biomarkers of Hepatocellular Carcinoma.

    abstract：:Time-series metabolomics studies can provide insight into the dynamics of disease development and facilitate the discovery of prospective biomarkers. To improve the performance of early risk identification, a new strategy for analyzing time-series data based on dynamic networks (ATSD-DN) in a systematic time dimension...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep32448

    authors： Huang X,Zeng J,Zhou L,Hu C,Yin P,Lin X

    更新日期：2016-08-31 00:00:00

  • Highly Sensitive Detection of Minimal Cardiac Ischemia using Positron Emission Tomography Imaging of Activated Platelets.

    abstract：:A reliable method for the diagnosis of minimal cardiac ischemia would meet a strong demand for the sensitive diagnosis of coronary artery disease in cardiac stress testing and risk stratification in patients with chest pain but unremarkable ECGs and biomarkers. We hypothesized that platelets accumulate early on in isc...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep38161

    authors： Ziegler M,Alt K,Paterson BM,Kanellakis P,Bobik A,Donnelly PS,Hagemeyer CE,Peter K

    更新日期：2016-12-02 00:00:00

  • Spatio-temporal distribution and acoustic characterization of haddock (Melanogrammus aeglefinus, Gadidae) calls in the Arctic fjord Kongsfjorden (Svalbard Islands).

    abstract：:In this study we analysed the acoustic properties and presence of haddock calls in the Arctic fjord Kongsfjorden (79° N-12° E, Svalbard Islands, Norway) in one year. Data were collected with three autonomous acoustic recorders located in the inner, middle, and outer parts of the fjord. The fjord is characterized by a ...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-020-75415-9

    authors： Buscaino G,Picciulin M,Canale DE,Papale E,Ceraulo M,Grammauta R,Mazzola S

    更新日期：2020-10-26 00:00:00

  • Hierarchically structured activated carbon for ultracapacitors.

    abstract：:To resolve the pore-associated bottleneck problem observed in the electrode materials used for ultracapacitors, which inhibits the transport of the electrolyte ions, we designed hierarchically structured activated carbon (HAC) by synthesizing a mesoporous silica template/carbon composite and chemically activating it t...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep21182

    authors： Kim MH,Kim KB,Park SM,Roh KC

    更新日期：2016-02-16 00:00:00