Abstract：
:Extensive mapping of neuronal connections in the central nervous system requires high-throughput µm-scale imaging of large volumes. In recent years, different approaches have been developed to overcome the limitations due to tissue light scattering. These methods are generally developed to improve the performance of a specific imaging modality, thus limiting comprehensive neuroanatomical exploration by multi-modal optical techniques. Here, we introduce a versatile brain clearing agent (2,2'-thiodiethanol; TDE) suitable for various applications and imaging techniques. TDE is cost-efficient, water-soluble and low-viscous and, more importantly, it preserves fluorescence, is compatible with immunostaining and does not cause deformations at sub-cellular level. We demonstrate the effectiveness of this method in different applications: in fixed samples by imaging a whole mouse hippocampus with serial two-photon tomography; in combination with CLARITY by reconstructing an entire mouse brain with light sheet microscopy and in translational research by imaging immunostained human dysplastic brain tissue.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Costantini I,Ghobril JP,Di Giovanna AP,Allegra Mascaro AL,Silvestri L,Müllenbroich MC,Onofri L,Conti V,Vanzi F,Sacconi L,Guerrini R,Markram H,Iannello G,Pavone FSdoi
10.1038/srep09808subject
Has Abstractpub_date
2015-05-07 00:00:00pages
9808issn
2045-2322pii
srep09808journal_volume
5pub_type
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