Abstract:
:Matcha and green tea catechins such as (-)-epicatechin (EC), (-)-epigallocatechin (EGC) and (-)-epigallocatechin gallate (EGCG) have long been studied for their antioxidant and health-promoting effects. Using specific fluorophores for H2S (AzMC) and polysulfides (SSP4) as well as IC-MS and UPLC-MS/MS-based techniques we here show that popular Japanese and Chinese green teas and select catechins all catalytically oxidize hydrogen sulfide (H2S) to polysulfides with the potency of EGC > EGCG > EG. This reaction is accompanied by the formation of sulfite, thiosulfate and sulfate, consumes oxygen and is partially inhibited by the superoxide scavenger, tempol, and superoxide dismutase but not mannitol, trolox, DMPO, or the iron chelator, desferrioxamine. We propose that the reaction proceeds via a one-electron autoxidation process during which one of the OH-groups of the catechin B-ring is autooxidized to a semiquinone radical and oxygen is reduced to superoxide, either of which can then oxidize HS- to thiyl radicals (HS•) which react to form hydrogen persulfide (H2S2). H2S oxidation reduces the B-ring back to the hydroquinone for recycling while the superoxide is reduced to hydrogen peroxide (H2O2). Matcha and catechins also concentration-dependently and rapidly produce polysulfides in HEK293 cells with the potency order EGCG > EGC > EG, an EGCG threshold of ~300 nM, and an EC50 of ~3 μM, suggesting green tea also acts as powerful pro-oxidant in vivo. The resultant polysulfides formed are not only potent antioxidants, but elicit a cascade of secondary cytoprotective effects, and we propose that many of the health benefits of green tea are mediated through these reactions. Remarkably, all green tea leaves constitutively contain small amounts of H2S2.
journal_name
Redox Bioljournal_title
Redox biologyauthors
Olson KR,Briggs A,Devireddy M,Iovino NA,Skora NC,Whelan J,Villa BP,Yuan X,Mannam V,Howard S,Gao Y,Minnion M,Feelisch Mdoi
10.1016/j.redox.2020.101731subject
Has Abstractpub_date
2020-10-01 00:00:00pages
101731issn
2213-2317pii
S2213-2317(20)30936-8journal_volume
37pub_type
杂志文章相关文献
Redox Biology文献大全abstract::NADPH oxidase 5 (NOX5) is a transmembrane signaling enzyme that produces superoxide in response to elevated cytosolic calcium. In addition to its association with numerous human diseases, NOX5 has recently been discovered to play crucial roles in the immune response and cardiovascular system. Details of NOX5 maturatio...
journal_title:Redox biology
pub_type: 杂志文章
doi:10.1016/j.redox.2020.101656
更新日期:2020-09-01 00:00:00
abstract::Glucose starvation is one of the major forms of metabolic stress in cancer cells. Deprivation of glucose impairs glycolysis and the pentose phosphate pathway, which elicits oxidative stress due to enhanced production of reactive oxygen species (ROS) and impaired antioxidant system, leading to redox imbalance and cell ...
journal_title:Redox biology
pub_type: 杂志文章,评审
doi:10.1016/j.redox.2019.101154
更新日期:2019-07-01 00:00:00
abstract:OBJECTIVE:Cellular repressor of E1A-stimulated genes (CREG), a vasculoprotective molecule, is significantly downregulated in atherosclerotic vessels through unclear mechanisms. While epigenetic regulation is involved in atherosclerosis development, it is not known if the CREG gene is epigenetically regulated. The aim o...
journal_title:Redox biology
pub_type: 杂志文章
doi:10.1016/j.redox.2020.101444
更新日期:2020-05-01 00:00:00
abstract::Hydrogen peroxide appears to be the key reactive oxygen species involved in redox signalling, but comparisons of the low concentrations of hydrogen peroxide that are calculated to exist within cells with those previously shown to activate common signalling events in vitro indicate that direct oxidation of key thiol gr...
journal_title:Redox biology
pub_type: 杂志文章,评审
doi:10.1016/j.redox.2020.101484
更新日期:2020-08-01 00:00:00
abstract::Fanconi Anemia (FA) is a disease characterized by bone marrow (BM) failure and aplastic anemia. In addition to a defective DNA repair system, other mechanisms are involved in its pathogenesis, such as defective mitochondrial metabolism, accumulation of lipids, and increment of oxidative stress production. To better un...
journal_title:Redox biology
pub_type: 杂志文章
doi:10.1016/j.redox.2020.101618
更新日期:2020-09-01 00:00:00
abstract::Glutathione (GSH), the most abundant vertebrate endogenous redox buffer, plays key roles in organogenesis and embryonic development, however, organ-specific GSH utilization during development remains understudied. Monochlorobimane (MCB), a dye conjugated with GSH by glutathione-s-transferase (GST) to form a fluorescen...
journal_title:Redox biology
pub_type: 杂志文章
doi:10.1016/j.redox.2019.101235
更新日期:2019-09-01 00:00:00
abstract::Transcription factors control the rate of transcription of genetic information from DNA to messenger RNA, by binding specific DNA sequences in promoter regions. Transcriptional gene control is a rate-limiting process that is tightly regulated and based on transient environmental signals which are translated into long-...
journal_title:Redox biology
pub_type: 杂志文章,评审
doi:10.1016/j.redox.2019.101104
更新日期:2019-02-01 00:00:00
abstract::Preeclampsia affects one in twelve of the 130 million pregnancies a year. The lack of an effective therapeutic to prevent or treat it is responsible for an annual global cost burden of 100 billion US dollars. Preeclampsia also affects these women later in life as it is a recognised risk factor for cardiovascular disea...
journal_title:Redox biology
pub_type: 杂志文章
doi:10.1016/j.redox.2020.101768
更新日期:2021-01-01 00:00:00
abstract::Cardiovascular disease is the leading cause of death and disability worldwide with increased oxidative stress and reduced NO bioavailability serving as key risk factors. For decades, elevation in protein abundance and enzymatic activity of xanthine oxidoreductase (XOR) under hypoxic/inflammatory conditions has been as...
journal_title:Redox biology
pub_type: 杂志文章
doi:10.1016/j.redox.2020.101447
更新日期:2020-07-01 00:00:00
abstract::Coronary artery disease (CAD) is the primary critical cardiovascular event. Endothelial cell and monocyte dysfunction with subsequent extravagant inflammation are the main causes of vessel damage in CAD. Thus, strategies that repress cell death and manage unsuitable pro-inflammatory responses in CAD are potential ther...
journal_title:Redox biology
pub_type: 杂志文章
doi:10.1016/j.redox.2017.05.027
更新日期:2017-10-01 00:00:00
abstract::Acute oxygen (O2) sensing is essential for individuals to survive under hypoxic conditions. The carotid body (CB) is the main peripheral chemoreceptor, which contains excitable and O2-sensitive glomus cells with O2-regulated ion channels. Upon exposure to acute hypoxia, inhibition of K+ channels is the signal that tri...
journal_title:Redox biology
pub_type: 杂志文章,评审
doi:10.1016/j.redox.2017.04.033
更新日期:2017-08-01 00:00:00
abstract::Reactive oxygen species (ROS) are generated during physiological bouts of synaptic activity and as a consequence of pathological conditions in the central nervous system. How neurons respond to and distinguish between ROS in these different contexts is currently unknown. In Drosophila mutants with enhanced JNK activit...
journal_title:Redox biology
pub_type: 杂志文章
doi:10.1016/j.redox.2020.101712
更新日期:2020-10-01 00:00:00
abstract::Hydroxytyrosol (HT) is a main phenolic component of olive oil. In this study, we investigated the safety and effects produced by HT purified (99.5%) from olive mill waste. HT was administered at a daily dosage of 45mg for 8 weeks to volunteers with mild hyperlipidemia (n=14). We measured markers of cardiovascular dise...
journal_title:Redox biology
pub_type: 临床试验,杂志文章
doi:10.1016/j.redox.2016.12.014
更新日期:2017-04-01 00:00:00
abstract::Oxidative stress resulting from the accumulation of high levels of reactive oxygen species is a salient feature of, and a well-recognised pathological factor for, diverse pathologies. One common mechanism for oxidative stress damage is via the disruption of intracellular ion homeostasis to induce cell death. TRPM2 is ...
journal_title:Redox biology
pub_type: 杂志文章,评审
doi:10.1016/j.redox.2020.101755
更新日期:2020-10-01 00:00:00
abstract::Hydrogen peroxide (H2O2) is an essential second intracellular messenger. To reach its targets in the cytosol, H2O2 must cross a membrane, a feat that requires aquaporins (AQP) endowed with 'peroxiporin' activity (AQP3, AQP8, AQP9). Here, we exploit different organelle-targeted H2O2-sensitive probes to show that also A...
journal_title:Redox biology
pub_type: 杂志文章
doi:10.1016/j.redox.2019.101326
更新日期:2020-01-01 00:00:00
abstract::The precise molecular events defining the complex role of oxidative stress in the inactivation of the cerebral sodium pump in radical-induced neurodegenerative diseases is yet to be fully clarified and thus still open. Herein we investigated the modulation of the activity of the cerebral transmembrane electrogenic enz...
journal_title:Redox biology
pub_type: 杂志文章
doi:10.1016/j.redox.2014.12.015
更新日期:2015-01-01 00:00:00
abstract::Culturing cells and tissues in vitro has provided valuable insights into the molecular mechanisms regulating redox signaling in cells with implications for medicine. However, standard culture techniques maintain mammalian cells in vitro under an artificial physicochemical environment such as ambient air and 5% CO2. Ox...
journal_title:Redox biology
pub_type: 杂志文章,评审
doi:10.1016/j.redox.2014.04.008
更新日期:2014-04-18 00:00:00
abstract::Reactivation of p53 and induction of tumor cell apoptosis (RITA) is a small molecule that blocks p53-MDM2 interaction, thereby reactivating p53 in tumors. RITA can induce exclusive apoptosis in cancer cells independently of the p53 pathway; however, the resistance of cancer cells remains a major drawback. Here, we fou...
journal_title:Redox biology
pub_type: 杂志文章
doi:10.1016/j.redox.2017.05.025
更新日期:2017-10-01 00:00:00
abstract::Macrophage recruitment and pro-inflammatory differentiation are hallmarks of various diseases, including infection and sepsis. Although studies suggest that mitochondria may regulate macrophage immune responses, it remains unclear whether mitochondrial mass affects macrophage pro-inflammatory differentiation. Here, we...
journal_title:Redox biology
pub_type: 杂志文章
doi:10.1016/j.redox.2020.101761
更新日期:2020-10-01 00:00:00
abstract::Ferroptosis is an outcome of metabolic disorders and closely linked to liver cancer. However, the mechanism underlying the fine regulation of ferroptosis in liver cancer remains unclear. Here, we have identified two categories of genes: ferroptosis up-regulated factors (FUF) and ferroptosis down-regulated factors (FDF...
journal_title:Redox biology
pub_type: 杂志文章
doi:10.1016/j.redox.2019.101211
更新日期:2019-06-01 00:00:00
abstract:BACKGROUND:The oxidation of the methionine adenosyltransferase (MAT) by the combined impact of peroxides contaminating parenteral nutrition (PN) and oxidized redox potential of glutathione is suspected to explain its inhibition observed in animals. A modification of MAT activity is suspected to be at origin of the PN-a...
journal_title:Redox biology
pub_type: 杂志文章
doi:10.1016/j.redox.2015.12.003
更新日期:2016-08-01 00:00:00
abstract::We previously reported that oncogenic KRAS activation of the PI3K/AKT pathway stimulates the remaining wild-type HRAS and NRAS proteins in a manner dependent upon both eNOS expression and C118 in HRAS and NRAS, which promoted tumor growth. Interestingly however, we recently found that loss of wild-type HRAS, NRAS, and...
journal_title:Redox biology
pub_type: 杂志文章
doi:10.1016/j.redox.2015.09.023
更新日期:2015-08-01 00:00:00
abstract::Compared to other organs, the brain is especially exposed to oxidative stress. In general, brains from young females tend to present lower oxidative damage in comparison to their male counterparts. This has been attributed to higher antioxidant defenses and a better mitochondrial function in females, which has been li...
journal_title:Redox biology
pub_type: 杂志文章,评审
doi:10.1016/j.redox.2020.101505
更新日期:2020-04-01 00:00:00
abstract::In the present study, we aimed to investigate the impact of permanent cystathionine-β-Synthase (CBS) gene knockdown in human telomerase reverse transcriptase (hTERT) immortalized human adipose-derived mesenchymal stem cells (ASC52telo) and in their capacity to differentiate into adipocytes. CBS gene KD in ASC52telo ce...
journal_title:Redox biology
pub_type: 杂志文章
doi:10.1016/j.redox.2020.101668
更新日期:2020-08-02 00:00:00
abstract::Ozone (O3) is a serious public health concern. Recent findings indicate that the damaging health effects of O3 extend to multiple systemic organ systems. Herein, we hypothesize that O3 inhalation will cause downstream alterations to the liver. To test this, male Sprague-Dawley rats were exposed to 0.5ppm O3 for 8h/day...
journal_title:Redox biology
pub_type: 杂志文章
doi:10.1016/j.redox.2013.11.006
更新日期:2014-01-01 00:00:00
abstract::In order to overcome intercellular variability and thereby effectively assess signal propagation in biological networks it is imperative to simultaneously quantify multiple biological observables in single living cells. While fluorescent biosensors have been the tool of choice to monitor the dynamics of protein intera...
journal_title:Redox biology
pub_type: 杂志文章
doi:10.1016/j.redox.2018.07.023
更新日期:2018-10-01 00:00:00
abstract::Protein disulfide isomerases (PDI) are a family of redox chaperones that catalyze formation or isomerization of disulfide bonds in proteins. Previous studies have shown that one member, PDIA3, interacts with influenza A virus (IAV) hemagglutinin (HA), and this interaction is required for efficient oxidative folding of...
journal_title:Redox biology
pub_type: 杂志文章
doi:10.1016/j.redox.2019.101129
更新日期:2019-04-01 00:00:00
abstract::Artesunate (ART) is a prominent anti-malarial with significant anti-cancer properties. Our previous studies showed that ART enhances lysosomal function and ferritin degradation, which was necessary for its anti-cancer properties. ART targeting to mitochondria also significantly improved its efficacy, but the effect of...
journal_title:Redox biology
pub_type: 杂志文章
doi:10.1016/j.redox.2018.07.025
更新日期:2018-10-01 00:00:00
abstract::The development of hypertension is associated with mitochondrial redox balance disruptions. NADP+-dependent isocitrate dehydrogenase 2 (IDH2) plays an important role in the maintenance of mitochondrial redox balance by producing mitochondrial NADPH, which is an essential cofactor in the reduction of glutathione (from ...
journal_title:Redox biology
pub_type: 杂志文章
doi:10.1016/j.redox.2020.101548
更新日期:2020-07-01 00:00:00
abstract::Hydrogen peroxide (H2O2) at moderate steady-state concentrations synergizes with TNF-α, leading to increased nuclear levels of NF-κB p65 subunit and to a cell-type specific up-regulation of a limited number of NF-κB-dependent genes. Here, we address how H2O2 achieves this molecular specificity. HeLa and MCF-7 cells we...
journal_title:Redox biology
pub_type: 杂志文章
doi:10.1016/j.redox.2013.05.004
更新日期:2013-06-21 00:00:00