Abstract：
:Bone marrow mesenchymal stem cells (BMSCs) are involved in cancer initiation and metastasis, and sometimes mediate cell communication by releasing exosomes and delivering microRNAs (miRNAs). The study aims to investigate the effects of exosomal hsa-miR-23b-3p derived from human BMSCs on intracranial aneurysm (IA). Firstly, human BMSCs-derived exosomes were extracted by ultra-high speed centrifugation. After clinical specimen collection, imbalance of T helper (Th) 17/Treg was found in patients with IA. Then, basilar artery aneurysm models were established and BMSCs-derived exosomes were isolated and identified. The results showed that BMSCs-derived exosomes improved pathological remodeling of IA wall, upregulated the contractile phenotype and inhibited the secretory phenotype of smooth muscle cells and reduced the number of Th17 cells to maintain the balance of Th17/Treg. In addition, human BMSCs-derived exosomes inhibited the activation of the phosphatidylinositol-3 kinase (PI3K)/protein kinase B (Akt)/nuclear factor-kappa B (NF-κB) signaling pathway and maintained Th17/Treg balance, which in turn interfered with aneurysm formation. Finally, the targeting relationship between hsa-miR-23b-3p and KLF5 was confirmed. We further noted that BMSCs-derived exosomal hsa-miR-23b-3p inhibited IA formation by targeting KLF5 through suppression of the PI3k/Akt/NF-κB signaling pathway. All in all, our study concluded that BMSCs-derived exosomal hsa-miR-23b-3p could maintain Th17/Treg balance by targeting KLF5 through suppression of the PI3k/Akt/NF-κB signaling pathway, thus inhibit IA formation.
journal_name
Brain Res Bulljournal_title
Brain research bulletinauthors
Sun X,Zheng X,Zhang X,Zhang Y,Luo Gdoi
10.1016/j.brainresbull.2020.09.003subject
Has Abstractpub_date
2020-12-01 00:00:00pages
305-315eissn
0361-9230issn
1873-2747pii
S0361-9230(20)30625-0journal_volume
165pub_type
杂志文章abstract：:Surgical removal of the olfactory bulbs (OB) was performed in mature male red-winged blackbirds, maintained under a short-day light regime. Bulbectomy caused hyperphagia, which was not accompanied by obesity. Bulbectomized (OBX) birds had incresaed thyroid follicular activity and had greater developed testes than sham...
journal_title：Brain research bulletin
pub_type： 杂志文章
doi：10.1016/s0361-9230(79)80011-8
更新日期：1979-05-01 00:00:00
abstract：:How deep brain stimulation (DBS) acts and how the brain responds to it remains unclear. To investigate the mechanisms involved, we analyzed changes in local field potentials from the subthalamic area (STN-LFPs) recorded through the deep brain macroelectrode during monopolar DBS of the subthalamic nucleus area (STN-DBS...
journal_title：Brain research bulletin
pub_type： 杂志文章
doi：10.1016/j.brainresbull.2008.01.023
更新日期：2008-07-30 00:00:00
abstract：:Diminished sensitivity to kainic acid (KA) lesioning action is present in the hippocampus of the lactating dam. Given that KA can alter blood-brain barrier (BBB) permeability, we investigated whether lactation would protect BBB permeability after treatment with this neurotoxin. Adult virgin or lactating (13-15 days pp...
journal_title：Brain research bulletin
pub_type： 杂志文章
doi：10.1016/j.brainresbull.2018.11.020
更新日期：2019-01-01 00:00:00
abstract：:Spinal NMDA receptors contribute to control of the cardiovascular system by the ventrolateral medulla. However, little is known about the contribution of these receptors to suprabulbar regulation of hemodynamics and sympathetic outflow. Hence, the involvement of spinal NMDA receptors in regulation of the cardiovascula...
journal_title：Brain research bulletin
pub_type： 杂志文章
doi：10.1016/0361-9230(93)90169-c
更新日期：1993-01-01 00:00:00
abstract：:To examine synaptic input and association of terminals containing dopamine and other transmitters to rat striatal nitric oxide synthase-expressing neurons, an electron microscopic study using tyrosine hydroxylase (TH) immunohistochemistry combined with histochemistry for NADPH-diaphorase (NADPHd) was performed. NADPHd...
journal_title：Brain research bulletin
pub_type： 杂志文章
doi：10.1016/0361-9230(96)00035-4
更新日期：1996-01-01 00:00:00
abstract：:Norepinephrine (NE) and dopamine (DA) levels and turnover were measured in 17 discrete brain regions of Swiss-Webster (NIH) mice made aggressive by prolonged isolation. The NE steady state level was significantly lower in olfactory tubercle and substantia nigra and significantly higher in the septal area of the aggres...
journal_title：Brain research bulletin
pub_type： 杂志文章
doi：10.1016/0361-9230(80)90287-7
更新日期：1980-01-01 00:00:00
abstract：:Locomotor performance of transgenic R6/2 mice carrying the Huntington's disease (HD) mutation was assessed using four different tasks, fixed speed rotarod, accelerating rotarod, Digigait and footprint test. The tasks were compared directly in age- and CAG repeat-matched R6/2 mice. Accelerating rotarod was more sensiti...
journal_title：Brain research bulletin
pub_type： 杂志文章
doi：10.1016/j.brainresbull.2008.10.007
更新日期：2009-03-30 00:00:00
abstract：:Several studies have demonstrated alterations in the dopamine (DA) system after traumatic brain injury (TBI). Additionally, the existence of significant sex-related differences in the dopaminergic system has long been recognized. Accordingly, the purpose of the present study was to investigate whether TBI would differ...
journal_title：Brain research bulletin
pub_type： 杂志文章
doi：10.1016/j.brainresbull.2016.05.010
更新日期：2016-06-01 00:00:00
abstract：:Chronic swimming training and phytotherapeutic supplementation are assumed to alleviate oxidative damage, and support cell survival in the brain. The effect of forced, chronic swimming training, and enriched lab chow containing 1% (w/w) dried nettle (Urtica dioica) leaf were investigated for oxidative stress, inflamma...
journal_title：Brain research bulletin
pub_type： 杂志文章
doi：10.1016/j.brainresbull.2005.02.028
更新日期：2005-05-30 00:00:00
abstract：:Administering increasing dosages of 5alpha-dihydrotestosterone propionate (DHTP) (0, 40, or 80 microgram/100 g in oil daily) to ovariectomized female rats treated concurrently with estradiol benzoate (EB) (1 microgram/100 g in oil daily) caused significant, equivalent reduction in lordotic responsiveness in rats maint...
journal_title：Brain research bulletin
pub_type： 杂志文章
doi：10.1016/0361-9230(81)90090-3
更新日期：1981-07-01 00:00:00
abstract：:Insulin-like growth factor-1 (IGF-1) and growth hormone (GH) have been suggested to promote memory and cognitive capabilities. In a recent publication we observed that GH increase the proportion of the NR2B subunit mRNA transcript of the NMDA receptor in rat hippocampus. NR2B has been suggested to be essential for spa...
journal_title：Brain research bulletin
pub_type： 杂志文章
doi：10.1016/j.brainresbull.2005.01.012
更新日期：2005-05-15 00:00:00
abstract：:Male and female rats were treated daily for 7 days with mazindol (5, 10, and 20 mg/kg), an anorectic drug, and tested in the open field. Mazindol developed sensitization to its locomotor stimulatory effect in both sexes on day 7 with a nondose-dependent pattern of response. However, the locomotor activity appeared to ...
journal_title：Brain research bulletin
pub_type： 杂志文章
doi：10.1016/0361-9230(94)90034-5
更新日期：1994-01-01 00:00:00
abstract：:Electrophysiologic response characteristics of mouse sciatic nerve and spinal cord were investigated following CNS infection with the temperature-sensitive (ts) vesicular stomatitis virus (VSV) mutant G31 KS5. Measurements were obtained before clinical symptoms of the virus-induced CNS disease appeared. Sciatic nerve ...
journal_title：Brain research bulletin
pub_type： 杂志文章
doi：10.1016/0361-9230(94)90001-9
更新日期：1994-01-01 00:00:00
abstract：:Many objective and quantitative methods have been developed to create a procedure or a device to prove, describe and quantify olfactory deficit and anosmia, especially after a head trauma. Electrophysiological testing throughout olfactoelectroencephalography (olfactoEEG) is based on brain activity desynchronisation, a...
journal_title：Brain research bulletin
pub_type： 杂志文章
doi：10.1016/j.brainresbull.2006.08.004
更新日期：2006-12-11 00:00:00
abstract：:Developing nigrostriatal axons and their perikarya have substantial quantities of dopamine (DA) before the axons reach their postsynaptic target. In order to investigate possible developmental effects of these stores of DA, we have depleted DA chronically during critical periods in the ontogeny of the nigrostriatal sy...
journal_title：Brain research bulletin
pub_type： 杂志文章
doi：10.1016/0361-9230(82)90169-1
更新日期：1982-07-01 00:00:00
abstract：:This study tested for the presence of androgen receptor-immunoreactivity in somatostatin, galanin, vasopressin, corticotropin-releasing hormone, and oxytocin neurons in the rat forebrain. The brains of adult male Sprague-Dawley rats were fixed with 4% paraformaldehyde. Androgen receptor was visualized in coronal secti...
journal_title：Brain research bulletin
pub_type： 杂志文章
doi：10.1016/0361-9230(94)90117-1
更新日期：1994-01-01 00:00:00
abstract：:Aged (25-27 months) Long-Evans female rats were distinguished according to whether they showed no significant impairment (AU), moderate impairment (AMI), or severe impairment (ASI) in a spatial reference-memory task. Young (3-5 months) rats served as controls. Electrically evoked overflow of tritium was assessed in hi...
journal_title：Brain research bulletin
pub_type： 杂志文章
doi：10.1016/s0361-9230(03)00042-x
更新日期：2003-05-15 00:00:00
abstract：:A high proportion of patients who have suffered a stroke also suffer from aphasia. Approximately half of those affected will remain in this state despite intensive language therapy. Non-invasive brain stimulation allows us to directly and focally stimulate areas of the brain. Repetitive transcranial magnetic stimulati...
journal_title：Brain research bulletin
pub_type： 杂志文章,评审
doi：10.1016/j.brainresbull.2013.07.005
更新日期：2013-09-01 00:00:00
abstract：:The Tac4 gene-derived hemokinin-1 (HK-1) is present in pain-related regions and activates the tachykinin NK1 receptor, but with binding site and signaling pathways different from Substance P (SP). NK1 receptor is involved in nociception, but our earlier data showed that it has no role in chronic neuropathic hyperalges...
journal_title：Brain research bulletin
pub_type： 杂志文章
doi：10.1016/j.brainresbull.2019.01.015
更新日期：2019-04-01 00:00:00
abstract：:We quantitated the activity of ornithine decarboxylase (ODC) in homogenates and subcellular fractions of inner ear tissues from the rat and guinea pig and demonstrate inhibition of cochlear ODC by the aminoglycoside neomycin. Subcellular fractionation showed the enzyme associated with the post-mitochondrial supernatan...
journal_title：Brain research bulletin
pub_type： 杂志文章
doi：10.1016/0361-9230(87)90056-6
更新日期：1987-12-01 00:00:00
abstract：:Motor neurons are endowed with intrinsic and conditional membrane properties that may shape the final motor output. In the first half of this paper we present data on the contribution of I(h), a hyperpolarization-activated inward cation current, to phase-transition in motor neurons during rhythmic firing. Motor neuron...
journal_title：Brain research bulletin
pub_type： 杂志文章,评审
doi：10.1016/s0361-9230(00)00398-1
更新日期：2000-11-15 00:00:00
abstract：:Cerebral ischemia is one of the leading causes of death and long-term disability worldwide. N-myc downstream-regulated gene 4 (NDRG4) is predominantly expressed in the brain as well as in the heart and has been reported to be involved in resistance to neuronal cell death caused by ischemic injury. However, the underly...
journal_title：Brain research bulletin
pub_type： 杂志文章
doi：10.1016/j.brainresbull.2018.12.010
更新日期：2019-03-01 00:00:00
abstract：:Dorsal lateral geniculate nucleus (dLGN) multiple-unit activity (MUA) and single visual evoked responses (VER) followed by afterdischarge (AD) were examined in lightly restrained albino rats. It was found that VER AD spike components are initially quite prominent, exceeding at times the amplitude of the broad surface ...
journal_title：Brain research bulletin
pub_type： 杂志文章
doi：10.1016/0361-9230(81)90102-7
更新日期：1981-07-01 00:00:00
abstract：:Plasma gonadotropins, prolactin and hypothalamic tyrosine hydroxylase (TH) activity were evaluated at 15 and 30 min after third ventricular injection of bombesin at doses of 100 or 1000 ng and secretin at doses of 1000 and 5000 ng in ovariectomized (OVX) unanesthetized rats. Bombesin had no effect on plasma gonadotrop...
journal_title：Brain research bulletin
pub_type： 杂志文章
doi：10.1016/0361-9230(83)90053-9
更新日期：1983-07-01 00:00:00
abstract：:Small GTPases of the rho family regulate the extensive rearrangements of the cytoskeleton that characterize neuronal differentiation. Citron kinase is a target molecule for activated rhoA, previously implicated in control of cytokinesis. We have found that, in addition, it could play an important role in modulating th...
journal_title：Brain research bulletin
pub_type： 杂志文章
doi：10.1016/s0361-9230(03)00058-3
更新日期：2003-05-30 00:00:00
abstract：:The putative behavioral effects of the enantiomers of BAY K 8644 and the behavioral responses to (+/-)-BAY K 8644 following chronic injection were assessed on motor function in mice. The interaction of the enantiomers of BAY K 8644 with mouse brain dihydropyridine binding sites was also evaluated. The calcium channel ...
journal_title：Brain research bulletin
pub_type： 杂志文章
doi：10.1016/0361-9230(88)90019-6
更新日期：1988-12-01 00:00:00
abstract：:A classical approach to the control of food consumption has been to assume separate mechanisms for the arousal to eat, on the one hand, and the satiation of feeding responses, on the other. The present paper is concerned with a single, and a comparatively simple, neuronal mechanism which is endowed with properties to ...
journal_title：Brain research bulletin
pub_type： 杂志文章
doi：10.1016/0361-9230(85)90008-5
更新日期：1985-10-01 00:00:00
abstract：:The primary visual pathway exhibits a symmetrical anatomical structure, initially arising from the left and right retinas, passing through the lateral geniculate nucleus, and finally projecting to the left and right primary visual cortices. However, to our knowledge, studies based on scalp EEG have not provided adequa...
journal_title：Brain research bulletin
pub_type： 杂志文章
doi：10.1016/j.brainresbull.2016.11.005
更新日期：2017-01-01 00:00:00
abstract：:In order to test the functional significance of noradrenergic neurotransmission for brain maturation, rat pups were injected daily with clonidine from 8-21 days of postnatal life. In adulthood, single cell responses to microiontophoretically applied neurotransmitters were studied in different layers of the hippocampus...
journal_title：Brain research bulletin
pub_type： 杂志文章
doi：10.1016/0361-9230(89)90212-8
更新日期：1989-10-01 00:00:00
abstract：:Neurodevelopmental disorders such as autism and schizophrenia involve social interaction problems and poor mentalizing abilities, associated with abnormal regional cerebral activity. Similar problems may be present in aggressive personality disorders and psychopathy. This paper reviews brain imaging data from research...
journal_title：Brain research bulletin
pub_type： 杂志文章,评审
doi：10.1016/j.brainresbull.2006.01.008
更新日期：2006-04-14 00:00:00
