Abstract：
:Liquid phase separation into two or more coexisting phases has emerged as a new paradigm for understanding subcellular organization, prebiotic life, and the origins of disease. The design principles underlying biomolecular phase separation have the potential to drive the development of novel liquid-based organelles and therapeutics, however, an understanding of how individual molecules contribute to emergent material properties, and approaches to directly manipulate phase dynamics are lacking. Here, using microrheology, we demonstrate that droplets of poly-arginine coassembled with mono/polynucleotides have approximately 100 fold greater viscosity than comparable lysine droplets, both of which can be finer tuned by polymer length. We find that these amino acid-level differences can drive the formation of coexisting immiscible phases with tunable formation kinetics and can be further exploited to trigger the controlled release of droplet components. Together, this work provides a novel mechanism for leveraging sequence-level components in order to regulate droplet dynamics and multiphase coexistence.
journal_name
Nat Communjournal_title
Nature communicationsauthors
Fisher RS,Elbaum-Garfinkle Sdoi
10.1038/s41467-020-18224-ysubject
Has Abstractpub_date
2020-09-15 00:00:00pages
4628issue
1issn
2041-1723pii
10.1038/s41467-020-18224-yjournal_volume
11pub_type
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