Facilitating microplastic quantification through the introduction of a cellulose dissolution step prior to oxidation: Proof-of-concept and demonstration using diverse samples from the Inner Oslofjord, Norway.

Abstract:

:Identifying and quantifying microplastic in marine samples can be facilitated by removing natural organic matter (NOM). Cellulosic material, like chitin, however, are a type of NOM that is resistant to chemical digestion, and difficult to eliminate from samples. To address this, a two-step digestion method was developed to remove or reduce cellulosic materials in diverse marine media. This method was applied to reference microplastics, reference cellulosic materials, and diverse marine samples from the Inner Oslofjord Norway. This included plankton, seabed sediments near a water treatment plant and driftline sand. The method was developed and tested for plastic particles >45 μm. The first-step was to pre-dissolve cellulosic materials using a mixture of urea:thiourea:NaOH. This was followed by an oxidative digestion step, here using H2O2 and NaOH. Most reference plastics were unaffected, except minor effects for PET and nylon. After sufficient repetitions, cellulosic materials in both reference and marine samples were largely removed. This method was compared to other digestion methods used for microplastic quantification, including single-step oxidation, alkaline treatment, acid treatment and enzymatic treatment. The results indicate that the pre-dissolution step greatly facilitates NOM and cellulosic material digestion for the purpose of microplastic quantification.

journal_name

Mar Environ Res

authors

Olsen LMB,Knutsen H,Mahat S,Wade EJ,Arp HPH

doi

10.1016/j.marenvres.2020.105080

subject

Has Abstract

pub_date

2020-10-01 00:00:00

pages

105080

eissn

0141-1136

issn

1879-0291

pii

S0141-1136(19)30882-7

journal_volume

161

pub_type

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