Abstract:
:Identifying and quantifying microplastic in marine samples can be facilitated by removing natural organic matter (NOM). Cellulosic material, like chitin, however, are a type of NOM that is resistant to chemical digestion, and difficult to eliminate from samples. To address this, a two-step digestion method was developed to remove or reduce cellulosic materials in diverse marine media. This method was applied to reference microplastics, reference cellulosic materials, and diverse marine samples from the Inner Oslofjord Norway. This included plankton, seabed sediments near a water treatment plant and driftline sand. The method was developed and tested for plastic particles >45 μm. The first-step was to pre-dissolve cellulosic materials using a mixture of urea:thiourea:NaOH. This was followed by an oxidative digestion step, here using H2O2 and NaOH. Most reference plastics were unaffected, except minor effects for PET and nylon. After sufficient repetitions, cellulosic materials in both reference and marine samples were largely removed. This method was compared to other digestion methods used for microplastic quantification, including single-step oxidation, alkaline treatment, acid treatment and enzymatic treatment. The results indicate that the pre-dissolution step greatly facilitates NOM and cellulosic material digestion for the purpose of microplastic quantification.
journal_name
Mar Environ Resjournal_title
Marine environmental researchauthors
Olsen LMB,Knutsen H,Mahat S,Wade EJ,Arp HPHdoi
10.1016/j.marenvres.2020.105080subject
Has Abstractpub_date
2020-10-01 00:00:00pages
105080eissn
0141-1136issn
1879-0291pii
S0141-1136(19)30882-7journal_volume
161pub_type
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