Developmental abnormalities and neurotoxicological effects of CuO NPs on the black sea urchin Arbacia lixula by embryotoxicity assay.

Abstract:

:The embryotoxicity of CuO NPs was evaluated in the black sea urchin Arbacia lixula embryos, by using 24-well plates. Fertilized eggs were exposed to five doses of CuO NPs ranging from 0.07 to 20 ppb, until pluteus stage. CuO NPs suspensions in artificial seawater formed agglomerates of 80-200 nm size, and copper uptake was 2.5-fold up in larvae exposed to high NP concentrations in respect to control. Developmental delay and morphological alteration, including skeletal abnormalities, were observed, as well as impairment in cholinergic and serotonergic nervous systems. These findings suggest the potential of CuO NPs to interfere with the normal neurotransmission pathways, thus affecting larval morphogenesis. Overall, the embryotoxicity tests are effective for evaluation of nanoparticle effects on the health of aquatic biota. Furthermore, as the black sea urchin A. lixula demonstrated to be vulnerable to NP exposure, it may be a valid bioindicator in marine biomonitoring and ecotoxicological programmes.

journal_name

Mar Environ Res

authors

Maisano M,Cappello T,Catanese E,Vitale V,Natalotto A,Giannetto A,Barreca D,Brunelli E,Mauceri A,Fasulo S

doi

10.1016/j.marenvres.2015.05.010

subject

Has Abstract

pub_date

2015-10-01 00:00:00

pages

121-7

eissn

0141-1136

issn

1879-0291

pii

S0141-1136(15)00077-X

journal_volume

111

pub_type

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