Abstract：
:Rapid and cost-effective methods to monitor the presence of diarrhetic shellfish poisoning (DSP) toxins in seawater samples in an easy and reliable manner are required to protect human health and avoid economic losses to shellfish industry. Immunoassays for the detection of okadaic acid (OA) and dinophysistoxin-1 and dinophysistoxin-2 are developed by immobilising OA on self-assembled monothiols or dithiols in an ordered and oriented way, providing an effective limit of detection of ∼1 ng OA equiv./mL seawater. The immunoassays are applied to the analysis of the particulate fraction of seawater samples from two Catalan harbours (NW Mediterranean) and samples collected periodically from the Galician Rias (E Atlantic), as well as a reference mussel sample. Results are in agreement with LC-MS/MS and the certified values. OA concentration in seawater correlates with Dinophysis cell abundance, with a 1-2 weeks lag. The immunoassays provide powerful high-throughput analytical methods potentially applicable as alternative monitoring tools.
journal_name
Mar Environ Resjournal_title
Marine environmental researchauthors
Leonardo S,Toldrà A,Rambla-Alegre M,Fernández-Tejedor M,Andree KB,Ferreres L,Campbell K,Elliott CT,O'Sullivan CK,Pazos Y,Diogène J,Campàs Mdoi
10.1016/j.marenvres.2017.11.004subject
Has Abstractpub_date
2018-02-01 00:00:00pages
6-14eissn
0141-1136issn
1879-0291pii
S0141-1136(17)30596-2journal_volume
133pub_type
杂志文章abstract：:The size structure of coral populations is the culmination of key demographic events, including recruitment, mortality and growth, thereby providing important insights to recent ecological dynamics. Importantly, the size structure of corals reflects both intrinsic (inherent life-history characteristics) and extrinsic ...
journal_title：Marine environmental research
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