Effects of tributyltin on salmon interrenal CYP11β, steroidogenic factor-1 and glucocorticoid receptor transcripts in the presence and absence of second messenger activator, forskolin.

Abstract:

:The mechanisms by which TBT produces modulations of the endocrine systems are not fully described. In this study, juvenile salmon were force-fed diet containing TBT (0: solvent control, 0.1, 1 and 10 mg/kg fish) for 72 h. Subsequently, fish exposed to solvent control and 10 mg/kg TBT were exposed to waterborne concentration of the adenyl cyclase stimulator forskolin (200 μg/L) for 2 and 4 h. Tissue and blood were sampled from individual fish (n=6). Gene expression patterns of CYP11β, steroidogenic factor-1 (SF-1), and glucocorticoid receptor (GlucR) were determined by qPCR. TBT generally decreased mRNA levels of CYP11β, GlucR and SF-1, compared to the solvent control and these effects were differentially modulated by the presence of forskolin. This study suggests that TBT may exert broader endocrine disrupting effects through possible modulation of cAMP/PKA second messenger systems.

journal_name

Mar Environ Res

authors

Kortner TM,Pavlikova N,Arukwe A

doi

10.1016/j.marenvres.2010.01.005

subject

Has Abstract

pub_date

2010-01-01 00:00:00

pages

S56-8

eissn

0141-1136

issn

1879-0291

pii

S0141-1136(10)00027-9

journal_volume

69 Suppl

pub_type

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