Abstract:
:Photoreceptors can segregate inner segment proteins from outer segment proteins. It is uncertain whether such a sorting process occurs during disc morphogenesis in rods, thereby resulting in an outer segment plasma membrane which differs compositionally from the discs. In this study, the plasma membranes of intact, purified frog rod outer segments (ROS) have been labeled with a peanut lectin (PNA), either with or without prior neuraminidase treatment of the ROS. Neuraminidase removes terminal sialic acid residues from membrane-bound sialoglycoconjugates. PNA was demonstrated to bind to the plasma membrane of ROS only after neuraminidase treatment, as detected by fluorescence light microscopy and electron microscopy. In order to identify the sialoglycoproteins responsible for this labeling, ROS proteins were resolved by polyacrylamide gel electrophoresis and transferred to nitrocellulose paper for incubation with PNA. A single, prominent band at 125 Kd bound PNA after neuraminidase treatment of ROS. This band represents a protein which is an integral component of the ROS plasma membrane and is not associated with the interphotoreceptor matrix or other potential contaminants. Selective degradation of ROS prior to neuraminidase treatment indicates that this sialoglycoprotein is absent from discs and, thus, can serve as a marker specific for ROS plasma membrane during fractionation studies.
journal_name
Invest Ophthalmol Vis Scijournal_title
Investigative ophthalmology & visual scienceauthors
Polans AS,Burton MDsubject
Has Abstractpub_date
1988-10-01 00:00:00pages
1523-32issue
10eissn
0146-0404issn
1552-5783journal_volume
29pub_type
杂志文章abstract:Purpose:To compare in vivo lamina cribrosa (LC) strains (deformations) following acute IOP elevation in healthy, glaucoma, and ocular hypertensive subjects. Methods:There were 20 healthy, 20 high-tension primary open-angle glaucoma (POAG), 16 primary angle-closure glaucoma (PACG), and 20 ocular hypertensive (OHT; with...
journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:10.1167/iovs.17-21982
更新日期:2018-01-01 00:00:00
abstract:PURPOSE:To examine the correlation between corneal acoustic impedance and Young's modulus in a canine eye model. METHODS:Twenty canine globes were recovered from healthy animals. Corneal acoustic impedance was measured in the intact globes using two methods: a quantitative ultrasound spectroscopy method and the reflec...
journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:10.1167/iovs.10-5723
更新日期:2011-02-09 00:00:00
abstract:PURPOSE:To identify major gangliosides - the sialated glycolipids - of corneal epithelium; to determine which specific gangliosides, if any, are synthesized in a higher amount or are downregulated during corneal epithelial cell migration; and to determine what role, if any, they play in the modulation of corneal epithe...
journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:
更新日期:1996-03-01 00:00:00
abstract:PURPOSE:To investigate the neuroprotective effects of brain-derived neurotrophic factor (BDNF) against potassium cyanide (KCN)-induced retinal damage. METHODS:Rats were injected intravitreally with iodinated BDNF. Two days later, eyeballs were dissected into various parts, and the level of radioactivity in each part w...
journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:
更新日期:1999-08-01 00:00:00
abstract:PURPOSE:Hormone diseases induce changes in the lacrimal gland (LG) and ocular surface (OS). Thyroid hormone (TH) induces cell proliferation and lipid metabolism through the activation of TH receptors. The aim of the present study was to evaluate the location and comparative expression of TH receptor beta-1 (Thrb) in LG...
journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:10.1167/iovs.06-1309
更新日期:2007-07-01 00:00:00
abstract:PURPOSE:We examined changes of the central nervous system in patients with advanced primary open-angle glaucoma (POAG). METHODS:The clinical observational study included 15 patients with bilateral advanced POAG and 15 healthy normal control subjects, matched for age and sex with the study group. Retinal nerve fiber l...
journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:10.1167/iovs.12-9893
更新日期:2013-01-17 00:00:00
abstract:PURPOSE:Identification of novel proteins involved in retinal neovascularization may facilitate new and more effective molecular-based treatments for proliferative retinopathy. Tubedown-1 (Tbdn-1) is a novel protein that shows homology to the yeast acetyltransferase subunit NAT1 and copurifies with an acetyltransferase ...
journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:10.1167/iovs.03-1410
更新日期:2004-10-01 00:00:00
abstract:Purpose:To describe the phenotypic spectrum of retinal disease caused by the c.2991+1655A>G mutation in CEP290 and to compare disease severity between homozygous and compound heterozygous patients. Methods:Medical records were reviewed for best-corrected visual acuity (BCVA), age of onset, fundoscopy descriptions. Fov...
journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:10.1167/iovs.18-24817
更新日期:2018-09-04 00:00:00
abstract:PURPOSE:FGF-2 stimulates cell proliferation of rabbit corneal endothelial cells (rCECs) by degrading the cyclin-dependent kinase inhibitor p27(Kip1) (p27) through its phosphorylation mechanism. The authors investigated whether the cell proliferation of human CECs (hCECs) is also induced by FGF-2 stimulation through the...
journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:10.1167/iovs.11-8213
更新日期:2011-10-17 00:00:00
abstract:OBJECTIVE:To describe the variation in ocular biometry and its association with refraction in adult Mongolians. METHODS:The study included 1800 subjects, aged 40 years or more, who were selected in two Mongolian provinces-Hövsgöl and Omnögobi-to participate in this population survey. Axial length (AL) and its componen...
journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:10.1167/iovs.03-0456
更新日期:2004-03-01 00:00:00
abstract:PURPOSE:Large copy number variations (CNV) can contribute to increased burden for neurodegenerative diseases. In this study, we analyzed the genome-wide burden of large CNVs > 100 kb in primary open angle glaucoma (POAG), a neurodegenerative disease of the eye that is the largest cause of irreversible blindness. METHO...
journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:10.1167/iovs.14-14339
更新日期:2014-04-24 00:00:00
abstract:PURPOSE:To evaluate the efficiency of mitomycin C (MMC) in limiting the postoperative inflammatory response and scarring after strabismus surgery. METHODS:A prospective, two-stage, masked, controlled trial was conducted. In the first stage, the inflammatory response at the extraocular muscle reattachment site was incr...
journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:
更新日期:2000-10-01 00:00:00
abstract:PURPOSE:We evaluated the roles of tumor necrosis factor (TNF)-α in alkali-induced corneal neovascularization (CNV). METHODS:CNV was induced by alkali injury and compared in wild-type (WT) BALB/c mice, and TNF receptor 1-deficient (TNF-Rp55 KO) counterparts, or in mice treated with TNF-α antagonist and recombinant TNF-...
journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:10.1167/iovs.10-5548
更新日期:2012-06-14 00:00:00
abstract:PURPOSE:The purpose of the present study was to investigate the roles of caspase 1 and extracellular signal-regulated kinase (ERK) in inflammation-induced inhibition of lacrimal gland secretion. METHODS:Lacrimal gland inflammation was induced by injection of lipopolysaccharide (LPS; to study the role of caspase 1) or ...
journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:10.1167/iovs.08-1830
更新日期:2008-10-01 00:00:00
abstract::The definition and diagnostic criteria of dry eye disease (DED) proposed by the Japan Dry Eye Society and other countries are reviewed. The first definition and criteria of DED in Japan were proposed in 1995. In that report, DED was considered a disease of tears, which subsequently damaged ocular epithelia. The presen...
journal_title:Investigative ophthalmology & visual science
pub_type: 历史文章,杂志文章,评审
doi:10.1167/iovs.17-23475
更新日期:2018-11-01 00:00:00
abstract::The phosphatic metabolite content of specific anatomic regions within the crystalline lens was determined by phosphorus-31 nuclear magnetic resonance analyses performed on tissue perchloric acid (PCA) extracts. Anatomically distinct zones each were dissected from five sets of 10 porcine lenses, isolated, and frozen in...
journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:
更新日期:1985-04-01 00:00:00
abstract:Purpose:The activation of the urokinase-type plasminogen activator and its receptor system is associated with retinal diseases. Among peptide inhibitors of this system, UPARANT acts by preventing the onset of pathologic signs of neovascular ocular diseases. We investigated whether systemic UPARANT may act in a therapeu...
journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:10.1167/iovs.17-21593
更新日期:2017-06-01 00:00:00
abstract:PURPOSE:Interphotoreceptor retinoid-binding protein (IRBP) binds and transports retinoids and fatty acids in the interphotoreceptor space (IPS). To understand the relationship between the protein structure and its functions requires bulk quantities of human IRBP. The authors sought to produce recombinant human IRBP (rh...
journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:
更新日期:1994-09-01 00:00:00
abstract::To determine whether glaucoma selectively injures a particular size of optic nerve fiber (and its cell body), we induced chronic experimental glaucoma in one eye each of ten monkeys. With automated image analysis, the number and diameter of optic nerve fibers were compared between each glaucomatous eye and its normal ...
journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:
更新日期:1987-06-01 00:00:00
abstract:Purpose:Aging is a risk factor for dry eye disease. The aim of this study was to investigate if aging is associated with a range of signs of dry eye disease, including tear hyperosmolarity, reduced nerve density, and increased dendritic cell number, in mice. Method:Healthy C57BL/6 female mice, aged 2 months (young, n ...
journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:10.1167/iovs.19-26631
更新日期:2019-06-03 00:00:00
abstract:PURPOSE:To examine the distribution of anti-GQ1b, -GT1a, and -GD1b antibody binding in human extraocular muscles (EOMs), axial and limb muscles, and muscle spindles and thereby test the hypothesis that their distinctive ganglioside composition provides the molecular basis for selective involvement of EOMs and muscle sp...
journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:10.1167/iovs.08-3333
更新日期:2009-07-01 00:00:00
abstract:PURPOSE:The accessory lacrimal glands are assumed to contribute to the production of tear fluid, but little is known about their function. The goal of this study was to conduct an analysis of gene expression by glands of Wolfring that would provide a more complete picture of the function of these glands. METHODS:Gland...
journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:10.1167/iovs.12-10750
更新日期:2012-10-01 00:00:00
abstract:PURPOSE:The retina of the blind mole rat Spalax ehrenbergi was compared with other vertebrate photosensitive organs in an attempt to correlate its histologic organization with a presumptive nonvisual photoreceptor role. METHODS:The eyes of eight adult animals were analyzed by light and electron microscopy, using conve...
journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:
更新日期:2002-07-01 00:00:00
abstract:PURPOSE:To investigate possible protective effects of lens epithelium-derived growth factor (LEDGF) against photoreceptor death in light-damaged, Royal College of Surgeons (RCS) and P23H rhodopsin transgenic rats. METHODS:Twelve-week-old Sprague-Dawley (SD), 6-week-old RCS, and 10-day-old P23H (line 1, heterozygote) r...
journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:
更新日期:2001-04-01 00:00:00
abstract:PURPOSE:Best disease is a monogenic macular degeneration caused mainly by heterozygous mutations in the BEST1 gene. The objective was to characterize the molecular and clinical features of patients with the classical form of Best disease that is inherited in an autosomal recessive mode. METHODS:Clinical evaluation inc...
journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:10.1167/iovs.11-7174
更新日期:2011-07-18 00:00:00
abstract:PURPOSE:To explore the effect of lateral tension as a survival factor for retinal explants in vitro. The central nervous system (CNS) resides in a highly mechanical milieu. However, the importance of biomechanical homeostasis for normal CNS function has not been extensively explored. Diseases in which normal mechanical...
journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:10.1167/iovs.12-11420
更新日期:2013-03-13 00:00:00
abstract:PURPOSE:To investigate the distribution of inflammatory mediators such as interleukin (IL)-1beta and tumor necrosis factor (TNF)-alpha and angiogenic cytokines such as vascular endothelial growth factor (VEGF) and to identify their cellular source in surgically excised choroidal neovascular membranes (CNVMs) of various...
journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:
更新日期:1999-08-01 00:00:00
abstract:PURPOSE:Corneal allografts placed in human eyes at high risk often fail, and immune rejection is thought to be a major pathogenic factor. To understand the immunologic factors responsible for rejection in this instance, the authors have created "high-risk" eyes in mice by inducing corneal neovascularization. The author...
journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:
更新日期:1995-10-01 00:00:00
abstract::Previous studies have used Badal laser optometry to demonstrate significant variance in the distribution of tonic (or "dark-focus") resting positions of accommodation (TA) for groups of observers. This study investigates whether individual differences in TA are due to individual differences in autonomic tone of the ci...
journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:
更新日期:1985-07-01 00:00:00
abstract:PURPOSE:To determine the accuracy of a history of cataract and cataract surgery (self-report and for a sibling), and to determine which demographic, cognitive, and medical factors are predictive of an accurate history. METHODS:All participants in the Salisbury Eye Evaluation (SEE) project and their locally resident si...
journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:10.1167/iovs.02-1055
更新日期:2003-07-01 00:00:00