Abstract:
:We isolated a novel bacterial strain from a prokaryotic consortium associated to the psychrophilic marine ciliate Euplotes focardii, endemic of the Antarctic coastal seawater. The 16S rDNA sequencing and the phylogenetic analysis revealed the close evolutionary relationship to the Antarctic marine bacterium Marinomonas sp. BSw10506 and the sub antarctic Marinomonas polaris. We named this new strain Marinomonas sp. ef1. The optimal growth temperature in LB medium was 22 °C. Whole genome sequencing and analysis showed a reduced gene loss limited to regions encoding for transposases. Additionally, five genomic islands, e.g. DNA fragments that facilitate horizontal gene transfer phenomena, were identified. Two open reading frames predicted from the genomic islands coded for enzymes belonging to the Nitro-FMN-reductase superfamily. One of these, the putative NAD(P)H nitroreductase YfkO, has been reported to be involved in the bioreduction of silver (Ag) ions and the production of silver nanoparticles (AgNPs). After the Marinomonas sp. ef1 biomass incubation with 1 mM of AgNO3 at 22 °C, we obtained AgNPs within 24 h. The AgNPs were relatively small in size (50 nm) and had a strong antimicrobial activity against twelve common nosocomial pathogenic microorganisms including Staphylococcus aureus and two Candida strains. To our knowledge, this is the first report of AgNPs biosynthesis by a Marinomonas strain. This biosynthesis may play a dual role in detoxification from silver nitrate and protection from pathogens for the bacterium and potentially for the associated ciliate. Biosynthetic AgNPs also represent a promising alternative to conventional antibiotics against common pathogens.
journal_name
Sci Repjournal_title
Scientific reportsauthors
John MS,Nagoth JA,Ramasamy KP,Ballarini P,Mozzicafreddo M,Mancini A,Telatin A,Liò P,Giuli G,Natalello A,Miceli C,Pucciarelli Sdoi
10.1038/s41598-020-66878-xsubject
Has Abstractpub_date
2020-06-23 00:00:00pages
10218issue
1issn
2045-2322pii
10.1038/s41598-020-66878-xjournal_volume
10pub_type
杂志文章abstract::Microbial rhodopsin is a photoreceptor protein found in various bacteria and archaea, and it is considered to be a light-utilization device unique to heterotrophs. Recent studies have shown that several cyanobacterial genomes also include genes that encode rhodopsins, indicating that these auxiliary light-utilizing pr...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-73606-y
更新日期:2020-10-07 00:00:00
abstract::A novel process for improving tar yield through catalytic depolymerization of a typical lignite was put forward. The catalytic depolymerization of the lignite over CoCl2 and ZnCl2 catalyst in different concentrations were investigated by a Gray-King apparatus. The optimal catalyst concentrations of CoCl2 and ZnCl2 cat...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-14869-w
更新日期:2017-10-31 00:00:00
abstract::Two yellow luminescence bands related to different defects have been revealed in undoped GaN grown by hydride vapor phase epitaxy (HVPE). One of them, labeled YL1, has the zero-phonon line (ZPL) at 2.57 eV and the band maximum at 2.20 eV at low temperature. This luminescence band is the ubiquitous yellow band observed...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-26354-z
更新日期:2018-05-25 00:00:00
abstract::Solid solutions of Zr(Mo,W)2O7(OH,Cl)2∙2H2O with a preset ratio of components were prepared by a hydrothermal method. The chemical composition of the solutions was determined by energy dispersive X-ray spectroscopy (EDX). For all the samples of ZrMoxW2-xO7(OH,Cl)2∙2H2O (x = 0.0, 0.2, 0.4, 0.6, 0.8, 1.0, 1.2, 1.4, 1.6,...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-23529-6
更新日期:2018-03-28 00:00:00
abstract::Drying and aging paint dispersions display a wealth of complex phenomena that make their study fascinating yet challenging. To meet the growing demand for sustainable, high-quality paints, it is essential to unravel the microscopic mechanisms underlying these phenomena. Visualising the governing dynamics is, however, ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep34383
更新日期:2016-09-29 00:00:00
abstract::Seagrass meadows are among the four most productive marine ecosystems in the world. Zostera japonica (Z. japonica) is the most widely distributed species of seagrass in China. However, there is no reference genome or transcriptome available for Z. japonica, impeding progress in functional genomic and molecular ecology...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-51176-y
更新日期:2019-10-10 00:00:00
abstract::Glial fibrillary acidic protein expressing (GFAP+) glia modulate nociceptive neuronal activity in both the peripheral nervous system (PNS) and the central nervous system (CNS). Resident GFAP+ glia in dorsal root ganglia (DRG) known as satellite glial cells (SGCs) potentiate neuronal activity by releasing pro-inflammat...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-71073-z
更新日期:2020-08-25 00:00:00
abstract::Tin selenide has attracted much research interest due to its unprecedentedly high thermoelectric figure of merit (ZT). For real applications, it is desirable to increase the ZT value in the lower-temperature range, as the peak ZT value currently exists near the melting point. It is shown in this paper that the structu...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep26193
更新日期:2016-05-19 00:00:00
abstract::Respiratory diseases such as asthma, chronic obstructive pulmonary disease (COPD), and lung infections have critical consequences on mortality and morbidity in humans. The aims of the present study were to examine the mechanisms by which CXCL12 affects MUC1 transcription and airway inflammation, which depend on activa...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep27054
更新日期:2016-06-07 00:00:00
abstract::The use of micro- and nanodevices as multifunctional systems for biomedical applications has experienced an exponential growth during the past decades. Although a large number of studies have focused on the design and fabrication of new micro- and nanosystems capable of developing multiple functions, a deeper understa...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep11371
更新日期:2015-06-12 00:00:00
abstract::Multishelled NiO hollow microspheres for high-performance supercapacitors have been prepared and the formation mechanism has been investigated. By using resin microspheres to absorb Ni(2+) and subsequent proper calcinations, the shell numbers, shell spacing and exterior shell structure were facilely controlled via var...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep33241
更新日期:2016-09-12 00:00:00
abstract::Peach is an economically import fruit crop worldwide, and serves as a model species of the Rosaceae family as well. However, peach functional genomics studies are severely hampered due to its recalcitrance to regeneration and stable transformation. Here, we report a fast and efficient Agrobacterium rhizogenes-mediated...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-59626-8
更新日期:2020-02-18 00:00:00
abstract::Microorganisms associated with plants are highly diverse and can produce a large number of secondary metabolites, with antimicrobial, anti-parasitic and cytotoxic activities. We are particularly interested in exploring endophytes from medicinal plants found in the Pantanal, a unique and widely unexplored wetland in Br...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-21400-2
更新日期:2018-02-15 00:00:00
abstract::The structure and function of cellular membranes were sustained by redox-enzymes. We studied the interaction between the oxidative stress caused by excessive accumulation of ZnO-nanoparticles (ZnO-NPs) in plants and the role of redox-enzymes that can alleviate this stress. The crude callus extract from pomegranate, wh...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-76664-4
更新日期:2020-11-12 00:00:00
abstract::The quantification of hepatitis C virus (HCV) is essential for the management of chronic hepatitis C therapy. We have developed a fully automated microfluidic RT-qPCR system for rapid quantitative detection of HCV RNA in human EDTA-plasma and serum, and the performance of the method was assessed. The platform for the ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-64169-z
更新日期:2020-04-30 00:00:00
abstract::GDP-mannose pyrophosphorylase (GMP) catalyzed the formation of GDP-mannose, which serves as a donor for the biosynthesis of mannose-containing polysaccharides. In this study, three GMP genes from Dendrobium officinale (i.e., DoGMPs) were cloned and analyzed. The putative 1000 bp upstream regulatory region of these DoG...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep41010
更新日期:2017-02-08 00:00:00
abstract::Consistent cell preparation is a fundamental preliminary step for understanding complex cellular mechanisms in various cell-based research fields, including basic cell biology, cancer research, and tissue engineering. However, certain elusive factors, such as cellular de-differentiation and contamination with mycoplas...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-02001-x
更新日期:2017-05-15 00:00:00
abstract::Although various drugs, environmental pollutants and nanoparticles (NP) can cross the human placental barrier and may harm the developing fetus, knowledge on predictive placental transfer rates and the underlying transport pathways is mostly lacking. Current available in vitro placental transfer models are often inapp...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-23410-6
更新日期:2018-03-29 00:00:00
abstract::Ammonia-oxidizing archaea (AOA) are recently found to participate in the ammonia removal processes in wastewater treatment plants (WWTPs), similar to their bacterial counterparts. However, due to lack of cultivated AOA strains from WWTPs, their functions and contributions in these systems remain unclear. Here we repor...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep23747
更新日期:2016-03-31 00:00:00
abstract::X-ray free-electron lasers (XFELs) provide extremely bright and highly spatially coherent x-ray radiation with femtosecond pulse duration. Currently, they are widely used in biology and material science. Knowledge of the XFEL statistical properties during an experiment may be vitally important for the accurate interpr...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-19793-1
更新日期:2018-02-02 00:00:00
abstract::Plasmonics is a rapidly emerging platform for quantum state engineering with the potential for building ultra-compact and hybrid optoelectronic devices. Recent experiments have shown that despite the presence of decoherence and loss, photon statistics and entanglement can be preserved in single plasmonic systems. This...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep18313
更新日期:2015-12-16 00:00:00
abstract::Filth flies cause billions of dollars of losses annually to the animal production industry. Fluralaner is a relatively new pesticide currently sold for control of fleas, ticks, and mites on companion animals and poultry. We examined the efficacy of fluralaner against three species of filth flies. Insecticide-susceptib...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-68121-z
更新日期:2020-07-07 00:00:00
abstract::Bacterial communities from freshwater lakes are shaped by various factors such as nutrients, pH value, temperature, etc. Their compositions and relative abundances would undergo changes to adapt the changing environments, and in turn could affect the environments of freshwater lakes. Analyses of the freshwater lake's ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep30616
更新日期:2016-07-27 00:00:00
abstract::Microalgae and plankton can be a rich source of bioactivity. However, induction of secondary metabolite production in lab conditions can be difficult. One simple way of bypassing this issue is to collect biomass in the field and screen for bioactivity. Therefore, bulk net samples from three areas along the coast of no...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-15980-8
更新日期:2017-11-20 00:00:00
abstract::A synergetic approach of employing smooth mesoporous TiO2 microsphere (SμS-TiO2)-nanoparticulate TiO2 (np-TiO2) composite photoanode, and size and defect controlled CdSe quantum dots (QD) to achieve high efficiency (η) in a modified Grätzel solar cell, quantum dot sensitized whisperonic solar cells (QDSWSC), is report...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-27969-y
更新日期:2018-06-26 00:00:00
abstract::Colaughter-simultaneous laughter between two or more individuals-allows listeners across different cultures and languages to quickly evaluate affiliation within a social group. We examined whether infants are sensitive to acoustic information in colaughter that indicates affiliation, specifically whether they can diff...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-38954-4
更新日期:2019-03-11 00:00:00
abstract::Our study aimed to investigate the effect of Pap smear screening on stage at diagnosis of cervical cancer in a heterogeneous population of Thai women. Data was merged from the population-based cancer registry and screening registry based on unique identification numbers from 2006 to 2014. Patients being screened had l...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-52607-6
更新日期:2019-11-15 00:00:00
abstract::A correction to this article has been published and is linked from the HTML and PDF versions of this paper. The error has been fixed in the paper. ...
journal_title:Scientific reports
pub_type: 已发布勘误
doi:10.1038/s41598-018-31276-x
更新日期:2018-08-29 00:00:00
abstract::Recent reports on direct reprogramming of cancer cells (iPCs) which results in reduced tumorigenic potential has attributed the importance of epigenetics in tumorigenesis, but lacked genome-wide analysis. Here we describe successful generation of iPCs from non-small cell lung cancer (NSCLC) cell lines. Following repro...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep00592
更新日期:2012-01-01 00:00:00
abstract::DNA methylation on CpG sites is the most common epigenetic modification. Recently, methylation in a non-CpG context was found to occur widely on genomic DNA. Moreover, methylation of non-CpG sites is a highly controlled process, and its level may vary during cellular development. To study non-CpG methylation effects o...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep29119
更新日期:2016-07-07 00:00:00