Abstract：
:Peach is an economically import fruit crop worldwide, and serves as a model species of the Rosaceae family as well. However, peach functional genomics studies are severely hampered due to its recalcitrance to regeneration and stable transformation. Here, we report a fast and efficient Agrobacterium rhizogenes-mediated transformation system in peach. Various explants, including leaf, hypocotyl and shoot, were all able to induce transgenic hairy roots, with a transformation efficiency of over 50% for hypocotyl. Composite plants were generated by infecting shoots with A. rhizogenes to induce transgenic adventitious hairy roots. The composite plant system was successfully used to validate function of an anthocyanin-related regulatory gene PpMYB10.1 in transgenic hairy roots, and two downstream genes, PpUFGT and PpGST, were strongly activated. Our stable and reproductive A. rhizogenes-mediated transformation system provides an avenue for gene function assay, genetic engineering, and investigation of root-rhizosphere microorganism interaction in peach.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Xu S,Lai E,Zhao L,Cai Y,Ogutu C,Cherono S,Han Y,Zheng Bdoi
10.1038/s41598-020-59626-8subject
Has Abstractpub_date
2020-02-18 00:00:00pages
2836issue
1issn
2045-2322pii
10.1038/s41598-020-59626-8journal_volume
10pub_type
杂志文章abstract：:Chromatin remodeling has been newly established as an important cancer genome characterization and recent exome and whole-genome sequencing studies of hepatocellular carcinoma (HCC) showed that recurrent inactivating mutations in SWI/SNF subunits involved in the molecular basis of hepatocarcinogenesis. To test the hyp...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep04147
更新日期：2014-02-21 00:00:00
abstract：:Geographical origin determination of white rice has become the major issue of food industry. However, there is still lack of a high-throughput method for rapidly and reproducibly differentiating the geographical origins of commercial white rice. In this study, we developed a method that employed lipidomics and deep le...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-08892-0
更新日期：2017-08-17 00:00:00
abstract：:The product of the meiosis-expressed gene 1 (MEIG1) is found in the cell bodies of spermatocytes and recruited to the manchette, a structure unique to elongating spermatids, by Parkin co-regulated gene (PACRG). This complex is essential for targeting cargo to the manchette during sperm flagellum assembly. Here we show...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep18278
更新日期：2016-01-04 00:00:00
abstract：:In this study we present an image analysis methodology capable of quantifying morphological changes in tissue collagen fibril organization caused by pathological conditions. Texture analysis based on first-order statistics (FOS) and second-order statistics such as gray level co-occurrence matrix (GLCM) was explored to...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep02190
更新日期：2013-01-01 00:00:00
abstract：:It is generally assumed that an investment into cognitive abilities and their associated cost is particularly beneficial for long-lived species, as a prolonged lifespan allows to recoup the initial investment. However, ephemeral organisms possess astonishing cognitive abilities too. Invertebrates, for example, are cap...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-51652-5
更新日期：2019-10-23 00:00:00
abstract：:Isoliquiritigenin (ISL), a natural flavonoid extracted from licorice, has been demonstrated to exert attenuation of osteoclastogenesis and anti-angiogenesis activity in a wide variety of cells. Here, we first evaluated the effects of ISL on pathogenesis of osteoarthritis in a mouse model of OA. The data showed that IS...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-19162-y
更新日期：2018-01-29 00:00:00
abstract：:Fish reproductive success is linked to the ability of couples to mate and produce clutches that successfully hatch. Environmental stressors like high temperature and low pH can jeopardize this energetically costly process. In this study, exposure to high temperature and low pH was tested on a marine temperate species,...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-77906-1
更新日期：2020-11-30 00:00:00
abstract：:Inter-digital capacitors (IDCs) with aerosol-deposition (AD) high-k dielectric layer were compared via simulation and measurements of bare IDCs and AD IDCs at room temperature and subjected to a post-annealing process for realizing capacitive super-sensing applications. IDCs with thin AD films can provide higher capac...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-37416-7
更新日期：2019-01-24 00:00:00
abstract：:The unconventional myosin MYO18A that contains a PDZ domain is required for muscle integrity during zebrafish development. However, the mechanism by which it functions in myofibers is not clear. The presence of a PDZ domain suggests that MYO18A may interact with other partners to perform muscle-specific functions. Her...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep36768
更新日期：2016-11-08 00:00:00
abstract：:Various techniques are commonly used to produce nano-crystalline NiAl2O4 materials; however, their practical applications in the microwave region remain very limited. In this work, flexible substrates for metamaterials containing two different concentrations of NiAl2O4 (labelled Ni36 and Ni42) have been synthesised us...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-33295-0
更新日期：2018-10-08 00:00:00
abstract：:Surfaces with reversible wettability have broad applications but remain challenging since the switching process is usually energy intensive and complex. In this paper, a pyramid shaped Cu2S film with hierarchical micro/nanostructures is formed on a commercial copper mesh. This film is formed by a spontaneous redox sul...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-48952-1
更新日期：2019-08-28 00:00:00
abstract：:Self-healing is a capacity observed in most biological systems in which the healing processes are autonomously triggered after the damage. Inspired by this natural behavior, researchers believed that a synthetic material possessing similar self-recovery capability could also be developed. Albeit various intrinsic self...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep30804
更新日期：2016-08-22 00:00:00
abstract：:Although recent studies have shed insights on some of the potential causes of male infertility, new underlining molecular mechanisms still remain to be elucidated. Makorin-2 (Mkrn2) is an evolutionarily conserved gene whose biological functions are not fully known. We developed an Mrkn2 knockout mouse model to study t...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep39318
更新日期：2016-12-23 00:00:00
abstract：:Leiomyomas, adenomyosis, and endometriosis are reported to be risk factors for endometrial carcinoma (EC), and adenomyosis and endometriosis also for ovarian carcinoma (OC). We aimed to describe the prevalence of these conditions in EC patients with or without an OC diagnosis, and to investigate their relationship wit...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-59916-1
更新日期：2020-02-27 00:00:00
abstract：:Stroke incidence is a multifactorial disease and especially hyperhomocysteinemia is associated with a higher risk of stroke. Previous studies have reported a folate metabolism disorder associated with the MTHFR gene. We investigated four single nucleotide polymorphisms in the MTHFR 3'-UTR [2572 C > A (rs4846049), 4869...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-12668-x
更新日期：2017-09-29 00:00:00
abstract：:Neurovascular unit (NVU) is a basic unit in the brain, including neurons, glial cells, blood vessels and extracellular matrix. This concept implies the importance of a three-dimensional (3D) culture model including these cell types for investigating brain functions. However, little is known about the construction of a...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-17411-0
更新日期：2017-12-11 00:00:00
abstract：:Microbiome analysis using metagenomic sequencing has revealed a vast microbial diversity associated with plants. Identifying the molecular functions associated with microbiome-plant interaction is a significant challenge concerning the development of microbiome-derived technologies applied to agriculture. An alternati...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep29543
更新日期：2016-07-12 00:00:00
abstract：:Confirmatory tests for the diagnosis of brain death in addition to clinical findings may shorten observation time required in some countries and may add certainty to the diagnosis under specific circumstances. The practicability of Gadolinium-enhanced magnetic resonance angiography to confirm cerebral circulatory arre...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep03659
更新日期：2014-01-13 00:00:00
abstract：:The helicase Twinkle is indispensable for mtDNA replication in nucleoids. Previously, we showed that Twinkle is tightly membrane-associated even in the absence of mtDNA, which suggests that Twinkle is part of a membrane-attached replication platform. Here we show that this platform is a cholesterol-rich membrane struc...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep15292
更新日期：2015-10-19 00:00:00
abstract：:We have developed a plasma cell treatment device called Plasma-on-Chip that enables the real-time monitoring of a single cell culture during plasma treatment. The device consists of three parts: 1) microwells for cell culture, 2) a microplasma device for generating reactive oxygen and nitrogen species (RONS) for use i...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep41953
更新日期：2017-02-08 00:00:00
abstract：:We herein present a novel and sustainable technology for mercury recycling, with the maximum observed uptake capacity. Facile synthesis of the most efficient (~1.9 gg-1) nano-trap, made of montmorillonite-Fe-iron oxides, was performed to instantaneously remove mercury(II) ions from water. Elemental Hg was recovered fr...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-34172-6
更新日期：2018-11-02 00:00:00
abstract：:This study aimed to compare the accuracy of novel lipid indices, including the visceral adiposity index (VAI), lipid accumulation product (LAP), triglycerides and glucose (TyG) index, TyG-body mass index (TyG-BMI), and TyG-waist circumference (TyG-WC), in identifying insulin resistance and establish valid cutoff value...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-021-82053-2
更新日期：2021-01-27 00:00:00
abstract：:One of the greatest challenges in the field of biofabrication remains the discovery of suitable bioinks that satisfy physicochemical and biological requirements. Despite recent advances in tissue engineering and biofabrication, progress has been limited to the development of technologies using polymer-based materials....
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-59632-w
更新日期：2020-02-18 00:00:00
abstract：:Advanced glycation end-products (AGEs)-induced mesangial cell death is one of major causes of glomerulus dysfunction in diabetic nephropathy. Both endoplasmic reticulum (ER) stress and autophagy are adaptive responses in cells under environmental stress and participate in the renal diseases. The role of ER stress and ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep34167
更新日期：2016-09-26 00:00:00
abstract：:The effect on the reproductive system and fertility of living in a space environment remains unclear. Here, we caged 12 male mice under artificial gravity (≈1 gravity) (AG) or microgravity (MG) in the International Space Station (ISS) for 35 days, and characterized the male reproductive organs (testes, epididymides, a...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-50128-w
更新日期：2019-09-24 00:00:00
abstract：:Complex networks characterize the nature of internal/external interactions in real-world systems including social, economic, biological, ecological, and technological networks. Two issues keep as obstacles to fulfilling control of large-scale networks: structural controllability which describes the ability to guide a ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-22655-5
更新日期：2018-03-15 00:00:00
abstract：:New urease and nitrification inhibitors and polymer coatings were introduced in recent years, but their effects on N loss and plant N nutrition were scarcely examined in agronomic no-tillage production systems. A field experiment of urea treated with efficiency enhancers was conducted on no-tillage corn (Zea mays L.) ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-42912-5
更新日期：2019-04-29 00:00:00
abstract：:Video game play induces a generalized recovery of a range of spatial visual functions in the amblyopic brain. Here we ask whether video game play also alters temporal processing in the amblyopic brain. When visual targets are presented in rapid succession, correct identification of the first target (T1) can interfere ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep08483
更新日期：2015-02-26 00:00:00
abstract：:MicroRNAs (miRNAs) are proposed as potential biomarkers for the diagnosis of numerous diseases. Here, we performed a meta-analysis to evaluate the utility of faecal miRNAs as a non-invasive tool in colorectal cancer (CRC) screening. A systematic literature search, according to predetermined criteria, in five databases...
journal_title：Scientific reports
pub_type： 杂志文章,meta分析
doi：10.1038/s41598-019-45570-9
更新日期：2019-07-01 00:00:00
abstract：:Recent research has shown that the cerebellum is involved not only in motor control but also in higher-level activities, which are closely related to creativity. This study aimed to explore the role of the cerebellum in visual divergent thinking based on its intrinsic activity. To this end, we selected the resting-sta...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-73679-9
更新日期：2020-10-06 00:00:00
