Abstract:
:Peach is an economically import fruit crop worldwide, and serves as a model species of the Rosaceae family as well. However, peach functional genomics studies are severely hampered due to its recalcitrance to regeneration and stable transformation. Here, we report a fast and efficient Agrobacterium rhizogenes-mediated transformation system in peach. Various explants, including leaf, hypocotyl and shoot, were all able to induce transgenic hairy roots, with a transformation efficiency of over 50% for hypocotyl. Composite plants were generated by infecting shoots with A. rhizogenes to induce transgenic adventitious hairy roots. The composite plant system was successfully used to validate function of an anthocyanin-related regulatory gene PpMYB10.1 in transgenic hairy roots, and two downstream genes, PpUFGT and PpGST, were strongly activated. Our stable and reproductive A. rhizogenes-mediated transformation system provides an avenue for gene function assay, genetic engineering, and investigation of root-rhizosphere microorganism interaction in peach.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Xu S,Lai E,Zhao L,Cai Y,Ogutu C,Cherono S,Han Y,Zheng Bdoi
10.1038/s41598-020-59626-8subject
Has Abstractpub_date
2020-02-18 00:00:00pages
2836issue
1issn
2045-2322pii
10.1038/s41598-020-59626-8journal_volume
10pub_type
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