Abstract：
:Results from epidemiological and prospective studies indicate a close association between periodontitis and diabetes. However the mechanisms by which periodontal pathogens influence the development of prediabetes/diabetes are not clear. We previously reported that oral administration of a periodontal pathogen, Porphyromonas gingivalis (Pg) to WT mice results in insulin resistance, hyperinsulinemia, and glucose intolerance and that Pg translocates to the pancreas. In the current study, we determined the specific localization of Pg in relation to mouse and human pancreatic α- and β-cells using 3-D confocal and immunofluorescence microscopy and orthogonal analyses. Pg/gingipain is intra- or peri-nuclearly localized primarily in β-cells in experimental mice and also in human post-mortem pancreatic samples. We also identified bihormonal cells in experimental mice as well as human pancreatic samples. A low percentage of bihormonal cells has intracellular Pg in both humans and experimental mice. Our data show that the number of Pg translocated to the pancreas correlates with the number of bihormonal cells in both mice and humans. Our findings suggest that Pg/gingipain translocates to pancreas, particularly β-cells in both humans and mice, and this is strongly associated with emergence of bihormonal cells.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Ilievski V,Toth PT,Valyi-Nagy K,Valyi-Nagy T,Green SJ,Marattil RS,Aljewari HW,Wicksteed B,O'Brien-Simpson NM,Reynolds EC,Layden BT,Unterman TG,Watanabe Kdoi
10.1038/s41598-020-65828-xsubject
Has Abstractpub_date
2020-06-19 00:00:00pages
9976issue
1issn
2045-2322pii
10.1038/s41598-020-65828-xjournal_volume
10pub_type
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