Abstract:
:Fanconi Anemia is a rare genetic disease associated with DNA repair defects, congenital abnormalities and infertility. Most of FA pathway is evolutionary conserved, allowing dissection and mechanistic studies in simpler model systems such as Caenorhabditis elegans. In the present study, we employed C. elegans to better understand the role of FA group D2 (FANCD2) protein in vivo, a key player in promoting genome stability. We report that localization of FCD-2/FANCD2 is dynamic during meiotic prophase I and requires its heterodimeric partner FNCI-1/FANCI. Strikingly, we found that FCD-2 recruitment depends on SPO-11-induced double-strand breaks (DSBs) but not RAD-51-mediated strand invasion. Furthermore, exposure to DNA damage-inducing agents boosts FCD-2 recruitment on the chromatin. Finally, analysis of genetic interaction between FCD-2 and BRC-1 (the C. elegans orthologue of mammalian BRCA1) supports a role for these proteins in different DSB repair pathways. Collectively, we showed a direct involvement of FCD-2 at DSBs and speculate on its function in driving meiotic DNA repair.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Germoglio M,Valenti A,Gallo I,Forenza C,Santonicola P,Silva N,Adamo Adoi
10.1038/s41598-019-57096-1subject
Has Abstractpub_date
2020-01-09 00:00:00pages
103issue
1issn
2045-2322pii
10.1038/s41598-019-57096-1journal_volume
10pub_type
杂志文章abstract::The aim of this study is to explore the differentially expressed lncRNAs, which may have potential biological function and diagnostic value in colorectal cancer (CRC). Through integrated data mining, we finally identified nine differentially expressed lncRNAs and their potential mRNA targets. After a series of bioinfo...
journal_title:Scientific reports
pub_type: 临床试验,杂志文章
doi:10.1038/srep46572
更新日期:2017-04-13 00:00:00
abstract::This study was to evaluate the effectiveness of oral hygiene care in improving oral health- and health-related quality of life (OHRQoL and HRQoL) among patients receiving outpatient stroke rehabilitation. Subjects were randomized to: (1) a conventional oral hygiene care programme (COHCP) comprising a manual toothbrush...
journal_title:Scientific reports
pub_type: 杂志文章,随机对照试验
doi:10.1038/s41598-017-07666-y
更新日期:2017-08-09 00:00:00
abstract::The mangrove fern genus Acrostichum grows in the extremely unstable marine intertidal zone under harsh conditions, such as high salt concentrations, tidal rhythms and long-term climate changes. To explore the phylogenetic relationships and molecular mechanisms underlying adaptations in this genus, we sequenced the tra...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep35634
更新日期:2016-10-26 00:00:00
abstract::ANCA vasculitis encompasses several autoimmune conditions characterised by destruction of small vessels, inflammation of the respiratory tract and glomerulonephritis. Most patients harbour autoantibodies to myeloperoxidase (MPO) or proteinase 3 (PR3). Clinical and experimental data suggest that pathogenesis is driven ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep11888
更新日期:2015-07-07 00:00:00
abstract::Ammonia-oxidizing archaea (AOA) are recently found to participate in the ammonia removal processes in wastewater treatment plants (WWTPs), similar to their bacterial counterparts. However, due to lack of cultivated AOA strains from WWTPs, their functions and contributions in these systems remain unclear. Here we repor...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep23747
更新日期:2016-03-31 00:00:00
abstract::Recently, the first-order synchronization transition has been studied in systems of coupled phase oscillators. In this paper, we propose a framework to investigate the synchronization in the frequency-weighted Kuramoto model with all-to-all couplings. A rigorous mean-field analysis is implemented to predict the possib...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep21926
更新日期:2016-02-23 00:00:00
abstract::To study quantitatively replicative senescence as a tumor suppressor mechanism, we investigate the distribution of a growing clonal cell population restricted by Hayflick's limit. We find that in the biologically relevant range of parameters, if the imbalance between cell division and death is moderate or low (high de...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep17660
更新日期:2015-12-09 00:00:00
abstract::Combining strong magnetic anisotropy with strong exchange interaction is a long standing goal in the design of quantum magnets. The lanthanide complexes, while exhibiting a very strong ionic anisotropy, usually display a weak exchange coupling, amounting to only a few wavenumbers. Recently, an isostructural series of ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep24046
更新日期:2016-04-18 00:00:00
abstract::The annotation of genomic ranges of interest represents a recurring task for bioinformatics analyses. These ranges can originate from various sources, including peaks called for transcription factor binding sites (TFBS) or histone modification ChIP-seq experiments, chromatin structure and accessibility experiments (su...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-02464-y
更新日期:2017-06-01 00:00:00
abstract::Methamphetamine (METH) is a highly addictive psychostimulant that causes long-lasting effects in the brain and increases the risk of developing neurodegenerative diseases. The cellular and molecular effects of METH in the brain are functionally linked to alterations in glutamate levels. Despite the well-documented eff...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-80917-7
更新日期:2021-01-14 00:00:00
abstract::Bacterial proteins exported to the cell surface play key cellular functions. However, despite the interest to study the localisation of surface proteins such as adhesins, transporters or hydrolases, monitoring their dynamics in live imaging remains challenging, due to the limited availability of fluorescent probes rem...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-72498-2
更新日期:2020-09-25 00:00:00
abstract::Early brain development requires a tight orchestration between neural tube patterning and growth. How pattern formation and brain growth are coordinated is incompletely understood. Previously we showed that aristaless-related homeobox (ARX), a paired-like transcription factor, regulates cortical progenitor pool expans...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-36194-6
更新日期:2019-01-18 00:00:00
abstract::An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...
journal_title:Scientific reports
pub_type: 已发布勘误
doi:10.1038/s41598-020-68128-6
更新日期:2020-07-06 00:00:00
abstract::Red mud as a waste material is produced in large quantities by the aluminum industry. Heat activation has been used to enhance sorption capacity of red mud for its beneficial reuse as an effective sorbent. In this study, heat-activated red mud (HARM) was investigated for its Cd(II) sorption capacity under various proc...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-31967-5
更新日期:2018-09-10 00:00:00
abstract::Dentin sialoprotein (DSP) is a dentin extracellular matrix protein. It is involved in dental mesenchymal cell lineages and dentin formation through regulation of its target gene expression. DSP mutations cause dentin genetic diseases. However, mechanisms of DSP in controlling dental mesenchymal cell differentiation ar...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-00339-w
更新日期:2017-03-22 00:00:00
abstract::The possibility of producing transgenic cloned buffalos by nuclear transfer of fetal fibroblasts expressing enhanced green fluorescent protein (EGFP) was explored in this study. When buffalo fetal fibroblasts (BFFs) isolated from a male buffalo fetus were transfected with pEGFP-N1 (EGFP is driven by CMV and Neo is dri...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-25120-5
更新日期:2018-05-03 00:00:00
abstract::This corrects the article DOI: 10.1038/srep16188. ...
journal_title:Scientific reports
pub_type: 杂志文章,已发布勘误
doi:10.1038/srep46909
更新日期:2017-12-22 00:00:00
abstract::Prenatal stress is known to epigenetically program offspring physiology and behaviour, and may become a risk factor for adult complex diseases. To gain insight into the underlying environment-gene interactions, we used proton nuclear magnetic resonance spectroscopy to analyze urinary metabolomes of male and female ado...
journal_title:Scientific reports
pub_type: 临床试验,杂志文章
doi:10.1038/s41598-018-31230-x
更新日期:2018-08-28 00:00:00
abstract::Despite its intrinsic advantages, translation of laser induced breakdown spectroscopy for material identification has been often impeded by the lack of robustness of developed classification models, often due to the presence of spurious correlations. While a number of classifiers exhibiting high discriminatory power h...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep13169
更新日期:2015-08-19 00:00:00
abstract::Accumulating evidence suggests that neuroinflammatory processes are implicated in the initiation and progression of amyotrophic lateral sclerosis (ALS). Previous reports have demonstrated an increase in microgliosis and astrogliosis in the lumbar spinal cord of SOD1G93A transgenic mice before the onset of symptoms, a ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-53982-w
更新日期:2019-11-27 00:00:00
abstract::The new era of spintronics promises the development of nanodevices, where the electron spin will be used to store information and charge currents will be replaced by spin currents. For this, ferromagnetic semiconductors at room temperature are needed. We report on significant room-temperature spin polarization of EuS ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep01333
更新日期:2013-01-01 00:00:00
abstract::Auxin response factors (ARFs) are the transcription factors that regulate auxin responses in various aspects of plant growth and development. Although genome-wide analysis of ARF gene family has been done in some species, no information is available regarding ARF genes in chickpea. In this study, we identified 28 ARF ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-11327-5
更新日期:2017-09-07 00:00:00
abstract::Toxin-antitoxin (TA) systems were initially discovered as plasmid addiction systems on low-copy-number plasmids. Thousands of TA loci have since been identified on chromosomes, plasmids and mobile elements in bacteria and archaea with diverse roles in bacterial physiology and in maintenance of genetic elements. Here, ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-46318-1
更新日期:2019-07-08 00:00:00
abstract::Little work has been done on the prediction of malignancy risk in patients with subcentimeter thyroid nodule (TN) categorized as atypia/follicular lesion of undetermined significance (AUS/FLUS). We performed a retrospective analysis on the medical records of subcentimeter TNs whose initial fine-needle aspiration (FNA)...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-50597-z
更新日期:2019-10-18 00:00:00
abstract::This work describes a simple technique to produce porous ceramics with aligned porosity having very high permeability and specific surface area. SiOC-based compositions were processed from blends of three types of preceramic polymer and a catalyst, followed by curing and pyrolysis. The heating applied from the bottom ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep41049
更新日期:2017-01-20 00:00:00
abstract::Sphingopyxis granuli strain TFA is an α-proteobacterium that belongs to the sphingomonads, a group of bacteria well-known for its degradative capabilities and oligotrophic metabolism. Strain TFA is the only bacterium in which the mineralisation of the aromatic pollutant tetralin has been completely characterized at bi...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-65258-9
更新日期:2020-05-26 00:00:00
abstract::The stethoscope is used as first line diagnostic tool in assessment of patients with pulmonary symptoms. However, there is much debate about the diagnostic accuracy of this instrument. This meta-analysis aims to evaluate the diagnostic accuracy of lung auscultation for the most common respiratory pathologies. Studies ...
journal_title:Scientific reports
pub_type: 杂志文章,meta分析
doi:10.1038/s41598-020-64405-6
更新日期:2020-04-30 00:00:00
abstract::Second-harmonic generation (SHG) is the most specific label-free indicator of collagen accumulation in widespread pathologies such as fibrosis, and SHG-based measurements hold important potential for biomedical analyses. However, efficient collagen SHG scoring in histological slides is hampered by the limited depth-of...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep29863
更新日期:2016-07-20 00:00:00
abstract::Small oligomers formed early along human islet amyloid polypeptide (hIAPP) aggregation is responsible for the cell death in Type II diabetes. The epigallocatechin gallate (EGCG), a green tea extract, was found to inhibit hIAPP fibrillation. However, the inhibition mechanism and the conformational distribution of the s...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep33076
更新日期:2016-09-13 00:00:00
abstract::Combining chemotherapy and targeted therapies has resulted in an enhanced survival in metastatic colorectal cancer (mCRC) patients. However, the result of this palliative treatment in patients with metachronous peritoneal carcinomatosis (PC) remains unknown. The current population-based study aims to investigate the u...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep18632
更新日期:2015-12-21 00:00:00