Dietary delphinidin inhibits human colorectal cancer metastasis associating with upregulation of miR-204-3p and suppression of the integrin/FAK axis.

Abstract:

:Delphinidin is a flavonoid belonging to dietary anthocyanidin family that has been reported to possess diverse anti-tumoral activities. However, the effects of delphinidin on colorectal cancer (CRC) cells and the underlying mechanisms are not fully understood. Thus, we aimed to investigate the anti-cancer activity of delphinidin in CRC cells and the underlying molecular mechanisms. The effects of delphinidin on the viability, metastatic characteristics, signaling, and microRNA (miR) profile of human CRC cell lines used were analyzed. In vivo metastasis was also evaluated using xenograft animal models. Our findings showed that delphinidin (<100 μM) inhibited the colony formation of DLD-1, SW480, and SW620 cells, but non-significantly affected cell viability. Delphinidin also suppressed the migratory ability and invasiveness of the tested CRC cell lines, downregulated integrin αV/β3 expression, inhibited focal adhesion kinase (FAK)/Src/paxillin signaling, and interfered with cytoskeletal construction. Analysis of the miR expression profile revealed a number of miRs, particularly miR-204-3p, that were significantly upregulated and downregulated by delphinidin. Abolishing the expression of one upregulated miR, miR-204-3p, with an antagomir restored delphinidin-mediated inhibition of cell migration and invasiveness in DLD-1 cells as well as the αV/β3-integrin/FAK/Src axis. Delphinidin also inhibited the lung metastasis of DLD-1 cells in the xenograft animal model. Collectively, these results indicate that the migration and invasion of CRC cells are inhibited by delphinidin, and the mechanism may involve the upregulation of miR-204-3p and consequent suppression of the αV/β3-integrin/FAK axis. These findings suggest that delphinidin exerts anti-metastatic effects in CRC cells by inhibiting integrin/FAK signaling and indicate that miR-204-3p may play an important role in CRC metastasis.

journal_name

Sci Rep

journal_title

Scientific reports

authors

Huang CC,Hung CH,Hung TW,Lin YC,Wang CJ,Kao SH

doi

10.1038/s41598-019-55505-z

subject

Has Abstract

pub_date

2019-12-12 00:00:00

pages

18954

issue

1

issn

2045-2322

pii

10.1038/s41598-019-55505-z

journal_volume

9

pub_type

杂志文章
  • Dna is a New Target of Parp3.

    abstract::Most members of the poly(ADP-ribose)polymerase family, PARP family, have a catalytic activity that involves the transfer of ADP-ribose from a beta-NAD+-molecule to protein acceptors. It was recently discovered by Talhaoui et al. that DNA-dependent PARP1 and PARP2 can also modify DNA. Here, we demonstrate that DNA-depe...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-018-22673-3

    authors: Belousova EA,Ishchenko АA,Lavrik OI

    更新日期:2018-03-08 00:00:00

  • Photodynamic activation as a molecular switch to promote osteoblast cell differentiation via AP-1 activation.

    abstract::In photodynamic therapy (PDT), cells are impregnated with a photosensitizing agent that is activated by light irradiation, thereby photochemically generating reactive oxygen species (ROS). The amounts of ROS produced depends on the PDT dose and the nature of the photosensitizer. Although high levels of ROS are cytotox...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep13114

    authors: Kushibiki T,Tu Y,Abu-Yousif AO,Hasan T

    更新日期:2015-08-17 00:00:00

  • Cover crops and chicken grazing in a winter fallow field improve soil carbon and nitrogen contents and decrease methane emissions.

    abstract::Using symbiotic farming methods [cover crops and chicken grazing (+ C)] in a winter fallow field, we found that the soil organic matter and total nitrogen of the + C treatment were 5.2% and 26.6% higher, respectively, than those of a treatment with cover crops and no chicken grazing (- C). The annual rice grain yield ...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-69407-y

    authors: Zheng H,Zhou L,Wei J,Tang Q,Zou Y,Tang J,Xu H

    更新日期:2020-07-28 00:00:00

  • Author Correction: Diversity of gut microflora is required for the generation of B cell with regulatory properties in a skin graft model.

    abstract::An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...

    journal_title:Scientific reports

    pub_type: 已发布勘误

    doi:10.1038/s41598-020-62381-5

    authors: Alhabbab R,Blair P,Elgueta R,Stolarczyk E,Marks E,Becker PD,Ratnasothy K,Smyth L,Safinia N,Sharif-Paghaleh E,O'Connell S,Noelle RJ,Lord GM,Howard JK,Spencer J,Lechler RI,Lombardi G

    更新日期:2020-04-06 00:00:00

  • Health effect of agricultural pesticide use in China: implications for the development of GM crops.

    abstract::It is notable that the adoption of GM glyphosate-tolerant crops increases glyphosate use but reduces non-glyphosate herbicide use; and adoption of GM insect-resistant crops significantly reduces insecticide use. While the health hazard of pesticide use has been well documented, little literature evaluates the health e...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep34918

    authors: Zhang C,Hu R,Huang J,Huang X,Shi G,Li Y,Yin Y,Chen Z

    更新日期:2016-10-10 00:00:00

  • Integrin α11 cytoplasmic tail is required for FAK activation to initiate 3D cell invasion and ERK-mediated cell proliferation.

    abstract::Integrin α11β1 is a collagen-binding integrin, which is receiving increasing attention in the context of wound healing and fibrosis. Although α11β1 integrin displays similar collagen specificity to α2β1 integrin, both integrins have distinct in vivo functions. In this context, the contribution of α11 subunit cytoplasm...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-019-51689-6

    authors: Erusappan P,Alam J,Lu N,Zeltz C,Gullberg D

    更新日期:2019-10-25 00:00:00

  • Effects of veridical expectations on syntax processing in music: Event-related potential evidence.

    abstract::Numerous past studies have investigated neurophysiological correlates of music-syntactic processing. However, only little is known about how prior knowledge about an upcoming syntactically irregular event modulates brain correlates of music-syntactic processing. Two versions of a short chord sequence were presented re...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep19064

    authors: Guo S,Koelsch S

    更新日期:2016-01-19 00:00:00

  • Predictors for malignancy risk in subcentimeter thyroid nodules categorized as atypia/follicular lesion of undetermined significance by fine needle aspiration.

    abstract::Little work has been done on the prediction of malignancy risk in patients with subcentimeter thyroid nodule (TN) categorized as atypia/follicular lesion of undetermined significance (AUS/FLUS). We performed a retrospective analysis on the medical records of subcentimeter TNs whose initial fine-needle aspiration (FNA)...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-019-50597-z

    authors: Ding J,Jiang L,Fang J,Jiang Y,Zhu Y,Hua T,Yuan Y,Wu W

    更新日期:2019-10-18 00:00:00

  • Photoacoustic monitoring of tumor and normal tissue response to radiation.

    abstract::Hypoxia is a recognized characteristic of tumors that influences efficacy of radiotherapy (RT). Photoacoustic imaging (PAI) is a relatively new imaging technique that exploits the optical characteristics of hemoglobin to provide information on tissue oxygenation. In the present study, PAI based measures of tumor oxyge...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep21237

    authors: Rich LJ,Seshadri M

    更新日期:2016-02-17 00:00:00

  • Mechanical forces induce an asthma gene signature in healthy airway epithelial cells.

    abstract::Bronchospasm compresses the bronchial epithelium, and this compressive stress has been implicated in asthma pathogenesis. However, the molecular mechanisms by which this compressive stress alters pathways relevant to disease are not well understood. Using air-liquid interface cultures of primary human bronchial epithe...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-57755-8

    authors: Kılıç A,Ameli A,Park JA,Kho AT,Tantisira K,Santolini M,Cheng F,Mitchel JA,McGill M,O'Sullivan MJ,De Marzio M,Sharma A,Randell SH,Drazen JM,Fredberg JJ,Weiss ST

    更新日期:2020-01-22 00:00:00

  • Computational analysis of androgen receptor (AR) variants to decipher the relationship between protein stability and related-diseases.

    abstract::Although more than 1,000 androgen receptor (AR) mutations have been identified and these mutants are pathologically important, few theoretical studies have investigated the role of AR protein folding stability in disease and its relationship with the phenotype of the patients. Here, we extracted AR variant data from f...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-68731-7

    authors: Chen F,Chen X,Jiang F,Leng F,Liu W,Gui Y,Yu J

    更新日期:2020-07-21 00:00:00

  • Targeted analysis of polymorphic loci from low-coverage shotgun sequence data allows accurate genotyping of HLA genes in historical human populations.

    abstract::The highly polymorphic human leukocyte antigen (HLA) plays a crucial role in adaptive immunity and is associated with various complex diseases. Accurate analysis of HLA genes using ancient DNA (aDNA) data is crucial for understanding their role in human adaptation to pathogens. Here, we describe the TARGT pipeline for...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-64312-w

    authors: Pierini F,Nutsua M,Böhme L,Özer O,Bonczarowska J,Susat J,Franke A,Nebel A,Krause-Kyora B,Lenz TL

    更新日期:2020-04-30 00:00:00

  • Galanin receptor 3 attenuates inflammation and influences the gut microbiota in an experimental murine colitis model.

    abstract::The regulatory (neuro)peptide galanin and its three receptors (GAL1-3R) are involved in immunity and inflammation. Galanin alleviated inflammatory bowel disease (IBD) in rats. However, studies on the galanin receptors involved are lacking. We aimed to determine galanin receptor expression in IBD patients and to evalua...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-79456-y

    authors: Brunner SM,Reichmann F,Leitner J,Wölfl S,Bereswill S,Farzi A,Schneider AM,Klieser E,Neureiter D,Emberger M,Heimesaat MM,Weghuber D,Lang R,Holzer P,Kofler B

    更新日期:2021-01-12 00:00:00

  • Impaired hippocampal-cortical coupling but preserved local synchrony during sleep in APP/PS1 mice modeling Alzheimer's disease.

    abstract::Sleep, in addition to its brain restorative processes, plays an important role in memory transfer from its temporary store in the hippocampus to the more permanent storage in the neocortex. Alzheimer's disease (AD) affects memory and sleep. The aim of this study was to explore disturbances in global and local synchron...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-019-41851-5

    authors: Zhurakovskaya E,Ishchenko I,Gureviciene I,Aliev R,Gröhn O,Tanila H

    更新日期:2019-03-29 00:00:00

  • Bifunctional Mesoporous Carbon Nitride: Highly Efficient Enzyme-like Catalyst for One-pot Deacetalization-Knoevenagel Reaction.

    abstract::Recently, mesoporous carbon nitride (MCN) has aroused extensive interest for its potential applications in organocatalysis, photo- and electrochemistry and CO2 capture. However, further surface functionalization of MCN for advanced nanomaterials and catalysis still remains very challenging. Here we show that acidic ca...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep12901

    authors: Zhong L,Anand C,Lakhi KS,Lawrence G,Vinu A

    更新日期:2015-08-05 00:00:00

  • Stress-induced tunneling nanotubes support treatment adaptation in prostate cancer.

    abstract::Tunneling nanotubes (TNTs) are actin-based membranous structures bridging distant cells for intercellular communication. We define roles for TNTs in stress adaptation and treatment resistance in prostate cancer (PCa). Androgen receptor (AR) blockade and metabolic stress induce TNTs, but not in normal prostatic epithel...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-019-44346-5

    authors: Kretschmer A,Zhang F,Somasekharan SP,Tse C,Leachman L,Gleave A,Li B,Asmaro I,Huang T,Kotula L,Sorensen PH,Gleave ME

    更新日期:2019-05-24 00:00:00

  • Diversity and predictive metabolic pathways of the prokaryotic microbial community along a groundwater salinity gradient of the Pearl River Delta, China.

    abstract::Almost half of the groundwater in the Pearl River Delta (PRD) contains salt water originally derived from paleo-seawater due to the Holocene transgression, which then generates intense physicochemical gradients in the mixing zone between freshwater and saltwater. Although some studies have been conducted on the hydrol...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-018-35350-2

    authors: Sang S,Zhang X,Dai H,Hu BX,Ou H,Sun L

    更新日期:2018-11-23 00:00:00

  • Climate variability in the northern and southern Altai Mountains during the past 50 years.

    abstract::The Holocene drying trend in the northern Altai Mountains and the wetting trend in the southern Altai Mountains inferred from the paleoclimatic studies indicated it is needed to understand the modern climatic characters in this region. However, a detailed analysis of modern climate variations in the northern and south...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-018-21637-x

    authors: Zhang D,Yang Y,Lan B

    更新日期:2018-02-19 00:00:00

  • Gene expression profiling gut microbiota in different races of humans.

    abstract::The gut microbiome is shaped and modified by the polymorphisms of microorganisms in the intestinal tract. Its composition shows strong individual specificity and may play a crucial role in the human digestive system and metabolism. Several factors can affect the composition of the gut microbiome, such as eating habits...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep23075

    authors: Chen L,Zhang YH,Huang T,Cai YD

    更新日期:2016-03-15 00:00:00

  • Structural Basis for DNA Recognition of a Single-stranded DNA-binding Protein from Enterobacter Phage Enc34.

    abstract::Modern DNA sequencing capabilities have led to the discovery of a large number of new bacteriophage genomes, which are a rich source of novel proteins with an unidentified biological role. The genome of Enterobacter cancerogenus bacteriophage Enc34 contains several proteins of unknown function that are nevertheless co...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-017-15774-y

    authors: Cernooka E,Rumnieks J,Tars K,Kazaks A

    更新日期:2017-11-14 00:00:00

  • Competition structured a Late Cretaceous megaherbivorous dinosaur assemblage.

    abstract::Modern megaherbivore community richness is limited by bottom-up controls, such as resource limitation and resultant dietary competition. However, the extent to which these same controls impacted the richness of fossil megaherbivore communities is poorly understood. The present study investigates the matter with refere...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-019-51709-5

    authors: Mallon JC

    更新日期:2019-10-28 00:00:00

  • Tempered mlo broad-spectrum resistance to barley powdery mildew in an Ethiopian landrace.

    abstract::Recessive mutations in the Mlo gene confer broad spectrum resistance in barley (Hordeum vulgare) to powdery mildew (Blumeria graminis f. sp. hordei), a widespread and damaging disease. However, all alleles discovered to date also display deleterious pleiotropic effects, including the naturally occurring mlo-11 mutant ...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep29558

    authors: Ge X,Deng W,Lee ZZ,Lopez-Ruiz FJ,Schweizer P,Ellwood SR

    更新日期:2016-07-12 00:00:00

  • Multiaxial Lenticular Stress-Strain Relationship of Native Myocardium is Preserved by Infarct-Induced Natural Heart Regeneration in Neonatal Mice.

    abstract::Neonatal mice exhibit natural heart regeneration after myocardial infarction (MI) on postnatal day 1 (P1), but this ability is lost by postnatal day 7 (P7). Cardiac biomechanics intricately affect long-term heart function, but whether regenerated cardiac muscle is biomechanically similar to native myocardium remains u...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-63324-w

    authors: Wang H,Bennett-Kennett R,Paulsen MJ,Hironaka CE,Thakore AD,Farry JM,Eskandari A,Lucian HJ,Shin HS,Wu MA,Imbrie-Moore AM,Steele AN,Stapleton LM,Zhu Y,Dauskardt RH,Woo YJ

    更新日期:2020-04-30 00:00:00

  • Computational Classification Approach to Profile Neuron Subtypes from Brain Activity Mapping Data.

    abstract::The analysis of cell type-specific activity patterns during behaviors is important for better understanding of how neural circuits generate cognition, but has not been well explored from in vivo neurophysiological datasets. Here, we describe a computational approach to uncover distinct cell subpopulations from in vivo...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep12474

    authors: Li M,Zhao F,Lee J,Wang D,Kuang H,Tsien JZ

    更新日期:2015-07-27 00:00:00

  • Rheumatoid arthritis and risk for Alzheimer's disease: a systematic review and meta-analysis and a Mendelian Randomization study.

    abstract::Rheumatoid arthritis (RA) patients have been observed to be at a lower risk of developing Alzheimer's Disease (AD). Clinical trials have showed no relationship between nonsteroidal anti-inflammatory drug (NSAID) use and AD. The aim of this study was to establish if there is a causal link between RA and AD. A systemati...

    journal_title:Scientific reports

    pub_type: 杂志文章,meta分析

    doi:10.1038/s41598-017-13168-8

    authors: Policicchio S,Ahmad AN,Powell JF,Proitsi P

    更新日期:2017-10-09 00:00:00

  • IL1B-CGTC haplotype is associated with colorectal cancer in admixed individuals with increased African ancestry.

    abstract::Single-nucleotide polymorphisms (SNPs) in cytokine genes can affect gene expression and thereby modulate inflammation and carcinogenesis. However, the data on the association between SNPs in the interleukin 1 beta gene (IL1B) and colorectal cancer (CRC) are conflicting. We found an association between a 4-SNP haplotyp...

    journal_title:Scientific reports

    pub_type: 杂志文章,多中心研究

    doi:10.1038/srep41920

    authors: Sanabria-Salas MC,Hernández-Suárez G,Umaña-Pérez A,Rawlik K,Tenesa A,Serrano-López ML,Sánchez de Gómez M,Rojas MP,Bravo LE,Albis R,Plata JL,Green H,Borgovan T,Li L,Majumdar S,Garai J,Lee E,Ashktorab H,Brim H,Li L,

    更新日期:2017-02-03 00:00:00

  • Compact flexible multi-pass rotary delay line using spinning micro-machined mirrors.

    abstract::We propose a new method to extend the path length tunability of rotary delay-lines. This method was shown to achieve a duty cycle of >80% and repetition rates of over 40 kHz. The new method relies on a new multi-segmented micro-machined mirror and serial injection of a single reflection onto separate segments of this ...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-017-09576-5

    authors: Fleddermann R,Lee WM,Huang K,Campbell GT,Lam PK,Chow JH

    更新日期:2017-08-24 00:00:00

  • Circulating exosomal microRNAs reveal the mechanism of Fructus Meliae Toosendan-induced liver injury in mice.

    abstract::The toxicological mechanisms of liver injury caused by most traditional Chinese medicine (TCM) remain largely unknown. Due to the unique features, exosomal microRNAs (miRNAs) are currently attracting major interests to provide further insights into toxicological mechanisms. Thus, taking Fructus Meliae Toosendan as an ...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-018-21113-6

    authors: Zheng J,Yu L,Chen W,Lu X,Fan X

    更新日期:2018-02-12 00:00:00

  • Carotid Body-Mediated Chemoreflex Drive in The Setting of low and High Output Heart Failure.

    abstract::Enhanced carotid body (CB) chemoreflex function is strongly related to cardiorespiratory disorders and disease progression in heart failure (HF). The mechanisms underlying CB sensitization during HF are not fully understood, however previous work indicates blood flow per se can affect CB function. Then, we hypothesize...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-017-08142-3

    authors: Del Rio R,Andrade DC,Toledo C,Diaz HS,Lucero C,Arce-Alvarez A,Marcus NJ,Schultz HD

    更新日期:2017-08-14 00:00:00

  • Comparative Genomic and Regulatory Analyses of Natamycin Production of Streptomyces lydicus A02.

    abstract::Streptomyces lydicus A02 is used by industry because it has a higher natamycin-producing capacity than the reference strain S. natalensis ATCC 27448. We sequenced the complete genome of A02 using next-generation sequencing platforms, and to achieve better sequence coverage and genome assembly, we utilized single-molec...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-017-09532-3

    authors: Wu H,Liu W,Shi L,Si K,Liu T,Dong D,Zhang T,Zhao J,Liu D,Tian Z,Yue Y,Zhang H,Xuelian B,Liang Y

    更新日期:2017-08-22 00:00:00