Abstract：
:The mechanism by which enzymes arose from both abiotic and biological worlds remains an unsolved natural mystery. We postulate that an enzyme can emerge from any sequence of any functional polymer under permissive evolutionary conditions. To support this premise, we have arbitrarily chosen a 50-nucleotide DNA fragment encoding for the Bos taurus (cattle) albumin mRNA and subjected it to test-tube evolution to derive a catalytic DNA (DNAzyme) with RNA-cleavage activity. After only a few weeks, a DNAzyme with significant catalytic activity has surfaced. Sequence comparison reveals that seven nucleotides are responsible for the conversion of the noncatalytic sequence into the enzyme. Deep sequencing analysis of DNA pools along the evolution trajectory has identified individual mutations as the progressive drivers of the molecular evolution. Our findings demonstrate that an enzyme can indeed arise from a sequence of a functional polymer via permissive molecular evolution, a mechanism that may have been exploited by nature for the creation of the enormous repertoire of enzymes in the biological world today.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Gysbers R,Tram K,Gu J,Li Ydoi
10.1038/srep11405subject
Has Abstractpub_date
2015-06-19 00:00:00pages
11405issn
2045-2322pii
srep11405journal_volume
5pub_type
杂志文章abstract：:Definitive evidence for the direct band gap predicted for Wurtzite Gallium Phosphide (WZ GaP) nanowires has remained elusive due to the lack of strong band-to-band luminescence in these materials. In order to circumvent this problem, we successfully obtained large volume WZ GaP structures grown by nanoparticle-crawlin...
journal_title：Scientific reports
pub_type： 杂志文章
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更新日期：2020-05-13 00:00:00
abstract：:Marine macroalgal forests are highly productive and iconic ecosystems, which are seriously threatened by number of factors such as habitat destruction, overgrazing, ocean warming, and pollution. The effect of chronic, but low levels of pollutants on the long-term survival of the canopy-forming algae is not well unders...
journal_title：Scientific reports
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pub_type： 杂志文章
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更新日期：2020-11-24 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep23885
更新日期：2016-04-04 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
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更新日期：2017-02-07 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
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pub_type： 杂志文章,多中心研究
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更新日期：2018-10-02 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
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更新日期：2016-02-01 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
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更新日期：2016-07-27 00:00:00
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pub_type： 杂志文章
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更新日期：2019-08-29 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
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更新日期：2017-11-30 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
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更新日期：2018-02-01 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
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更新日期：2019-04-08 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
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pub_type： 杂志文章
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更新日期：2017-06-06 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep25055
更新日期：2016-04-26 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep05888
更新日期：2014-07-30 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
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更新日期：2020-05-22 00:00:00
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更新日期：2017-07-14 00:00:00
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pub_type： 杂志文章
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更新日期：2018-02-12 00:00:00
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更新日期：2019-05-06 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
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更新日期：2018-09-13 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep37277
更新日期：2016-11-17 00:00:00
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更新日期：2019-10-29 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
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更新日期：2019-03-27 00:00:00
