当前位置： SCI文献检索 > Nature Communications期刊下所有文献 > Allele-aware chromosome-level genome assembly and efficient transgene-free genome editing for the autotetraploid cultivated alfalfa.

Allele-aware chromosome-level genome assembly and efficient transgene-free genome editing for the autotetraploid cultivated alfalfa.

Abstract：

:Artificially improving traits of cultivated alfalfa (Medicago sativa L.), one of the most important forage crops, is challenging due to the lack of a reference genome and an efficient genome editing protocol, which mainly result from its autotetraploidy and self-incompatibility. Here, we generate an allele-aware chromosome-level genome assembly for the cultivated alfalfa consisting of 32 allelic chromosomes by integrating high-fidelity single-molecule sequencing and Hi-C data. We further establish an efficient CRISPR/Cas9-based genome editing protocol on the basis of this genome assembly and precisely introduce tetra-allelic mutations into null mutants that display obvious phenotype changes. The mutated alleles and phenotypes of null mutants can be stably inherited in generations in a transgene-free manner by cross pollination, which may help in bypassing the debate about transgenic plants. The presented genome and CRISPR/Cas9-based transgene-free genome editing protocol provide key foundations for accelerating research and molecular breeding of this important forage crop.

journal_name

Nat Commun

journal_title

Nature communications

authors

Chen H,Zeng Y,Yang Y,Huang L,Tang B,Zhang H,Hao F,Liu W,Li Y,Liu Y,Zhang X,Zhang R,Zhang Y,Li Y,Wang K,He H,Wang Z,Fan G,Yang H,Bao A,Shang Z,Chen J,Wang W,Qiu Q

doi

10.1038/s41467-020-16338-x

subject

Has Abstract

pub_date

2020-05-19 00:00:00

pages

2494

issue

1

issn

2041-1723

pii

10.1038/s41467-020-16338-x

journal_volume

11

pub_type

杂志文章

相关文献

Nature Communications文献大全
  • Sphingosine-1-phosphate receptor 3 promotes leukocyte rolling by mobilizing endothelial P-selectin.

    abstract：:Sphingosine-1-phosphate (S1P) participates in inflammation; however, its role in leukocyte rolling is still unclear. Here we use intravital microscopy in inflamed mouse cremaster muscle venules and human endothelial cells to show that S1P contributes to P-selectin-dependent leukocyte rolling through endothelial S1P re...

    journal_title：Nature communications

    pub_type： 杂志文章

    doi：10.1038/ncomms7416

    authors： Nussbaum C,Bannenberg S,Keul P,Gräler MH,Gonçalves-de-Albuquerque CF,Korhonen H,von Wnuck Lipinski K,Heusch G,de Castro Faria Neto HC,Rohwedder I,Göthert JR,Prasad VP,Haufe G,Lange-Sperandio B,Offermanns S,Sperandio M,Lev

    更新日期：2015-04-02 00:00:00

  • Vanadium dioxide nanowire-based microthermometer for quantitative evaluation of electron beam heating.

    abstract：:Temperature measurement is critical for many technological applications and scientific experiments, and different types of thermometers have been developed to detect temperature at macroscopic length scales. However, quantitative measurement of the temperature of nanostructures remains a challenge. Here, we show a new...

    journal_title：Nature communications

    pub_type： 杂志文章

    doi：10.1038/ncomms5986

    authors： Guo H,Khan MI,Cheng C,Fan W,Dames C,Wu J,Minor AM

    更新日期：2014-10-13 00:00:00

  • Rethinking Indian monsoon rainfall prediction in the context of recent global warming.

    abstract：:Prediction of Indian summer monsoon rainfall (ISMR) is at the heart of tropical climate prediction. Despite enormous progress having been made in predicting ISMR since 1886, the operational forecasts during recent decades (1989-2012) have little skill. Here we show, with both dynamical and physical-empirical models, t...

    journal_title：Nature communications

    pub_type： 杂志文章

    doi：10.1038/ncomms8154

    authors： Wang B,Xiang B,Li J,Webster PJ,Rajeevan MN,Liu J,Ha KJ

    更新日期：2015-05-18 00:00:00

  • Atypical chemoreceptor arrays accommodate high membrane curvature.

    abstract：:The prokaryotic chemotaxis system is arguably the best-understood signaling pathway in biology. In all previously described species, chemoreceptors organize into a hexagonal (P6 symmetry) extended array. Here, we report an alternative symmetry (P2) of the chemotaxis apparatus that emerges from a strict linear organiza...

    journal_title：Nature communications

    pub_type： 杂志文章

    doi：10.1038/s41467-020-19628-6

    authors： Muok AR,Ortega DR,Kurniyati K,Yang W,Maschmann ZA,Sidi Mabrouk A,Li C,Crane BR,Briegel A

    更新日期：2020-11-13 00:00:00

  • Spatially inhomogeneous competition between superconductivity and the charge density wave in YBa2Cu3O6.67.

    abstract：:The charge density wave in the high-temperature superconductor YBa2Cu3O7-x (YBCO) has two different ordering tendencies differentiated by their c-axis correlations. These correspond to ferro- (F-CDW) and antiferro- (AF-CDW) couplings between CDWs in neighbouring CuO2 bilayers. This discovery has prompted several funda...

    journal_title：Nature communications

    pub_type： 杂志文章

    doi：10.1038/s41467-020-14536-1

    authors： Choi J,Ivashko O,Blackburn E,Liang R,Bonn DA,Hardy WN,Holmes AT,Christensen NB,Hücker M,Gerber S,Gutowski O,Rütt U,Zimmermann MV,Forgan EM,Hayden SM,Chang J

    更新日期：2020-02-20 00:00:00

  • Highly multiplexed imaging of single cells using a high-throughput cyclic immunofluorescence method.

    abstract：:Single-cell analysis reveals aspects of cellular physiology not evident from population-based studies, particularly in the case of highly multiplexed methods such as mass cytometry (CyTOF) able to correlate the levels of multiple signalling, differentiation and cell fate markers. Immunofluorescence (IF) microscopy add...

    journal_title：Nature communications

    pub_type： 杂志文章

    doi：10.1038/ncomms9390

    authors： Lin JR,Fallahi-Sichani M,Sorger PK

    更新日期：2015-09-24 00:00:00

  • A bioinspired hydrogen bond-triggered ultrasensitive ionic mechanoreceptor skin.

    abstract：:Biological cellular structures have inspired many scientific disciplines to design synthetic structures that can mimic their functions. Here, we closely emulate biological cellular structures in a rationally designed synthetic multicellular hybrid ion pump, composed of hydrogen-bonded [EMIM+][TFSI-] ion pairs on the s...

    journal_title：Nature communications

    pub_type： 杂志文章

    doi：10.1038/s41467-019-11973-5

    authors： Amoli V,Kim JS,Jee E,Chung YS,Kim SY,Koo J,Choi H,Kim Y,Kim DH

    更新日期：2019-09-05 00:00:00

  • Acetylation of histone H4 lysine 5 and 12 is required for CENP-A deposition into centromeres.

    abstract：:Centromeres are specified epigenetically through the deposition of the centromere-specific histone H3 variant CENP-A. However, how additional epigenetic features are involved in centromere specification is unknown. Here, we find that histone H4 Lys5 and Lys12 acetylation (H4K5ac and H4K12ac) primarily occur within the...

    journal_title：Nature communications

    pub_type： 杂志文章

    doi：10.1038/ncomms13465

    authors： Shang WH,Hori T,Westhorpe FG,Godek KM,Toyoda A,Misu S,Monma N,Ikeo K,Carroll CW,Takami Y,Fujiyama A,Kimura H,Straight AF,Fukagawa T

    更新日期：2016-11-04 00:00:00

  • Male-female communication triggers calcium signatures during fertilization in Arabidopsis.

    abstract：:Cell-cell communication and interaction is critical during fertilization and triggers free cytosolic calcium ([Ca2+]cyto) as a key signal for egg activation and a polyspermy block in animal oocytes. Fertilization in flowering plants is more complex, involving interaction of a pollen tube with egg adjoining synergid ce...

    journal_title：Nature communications

    pub_type： 杂志文章

    doi：10.1038/ncomms5645

    authors： Denninger P,Bleckmann A,Lausser A,Vogler F,Ott T,Ehrhardt DW,Frommer WB,Sprunck S,Dresselhaus T,Grossmann G

    更新日期：2014-08-22 00:00:00

  • Orbital-selective spin texture and its manipulation in a topological insulator.

    abstract：:Topological insulators represent a new quantum state of matter that are insulating in the bulk but metallic on the edge or surface. In the Dirac surface state, it is well-established that the electron spin is locked with the crystal momentum. Here we report a new phenomenon of the spin texture locking with the orbital...

    journal_title：Nature communications

    pub_type： 杂志文章

    doi：10.1038/ncomms4382

    authors： Xie Z,He S,Chen C,Feng Y,Yi H,Liang A,Zhao L,Mou D,He J,Peng Y,Liu X,Liu Y,Liu G,Dong X,Yu L,Zhang J,Zhang S,Wang Z,Zhang F,Yang F,Peng Q,Wang X,Chen C,Xu Z,Zhou XJ

    更新日期：2014-02-28 00:00:00

  • Author Correction: Expression-based drug screening of neural progenitor cells from individuals with schizophrenia.

    abstract：:In the originally published version of this Article, the affiliation details for Eric E. Schadt and Radoslav Savic incorrectly omitted 'Sema4, a Mount Sinai venture, Stamford, Connecticut, USA'. This has been corrected in both the PDF and HTML versions of the Article. ...

    journal_title：Nature communications

    pub_type： 杂志文章,已发布勘误

    doi：10.1038/s41467-018-07326-3

    authors： Readhead B,Hartley BJ,Eastwood BJ,Collier DA,Evans D,Farias R,He C,Hoffman G,Sklar P,Dudley JT,Schadt EE,Savić R,Brennand KJ

    更新日期：2018-11-19 00:00:00

  • In vivo imaging of clock gene expression in multiple tissues of freely moving mice.

    abstract：:Clock genes are expressed throughout the body, although how they oscillate in unrestrained animals is not known. Here, we show an in vivo imaging technique that enables long-term simultaneous imaging of multiple tissues. We use dual-focal 3D tracking and signal-intensity calibration to follow gene expression in a targ...

    journal_title：Nature communications

    pub_type： 杂志文章

    doi：10.1038/ncomms11705

    authors： Hamada T,Sutherland K,Ishikawa M,Miyamoto N,Honma S,Shirato H,Honma K

    更新日期：2016-06-10 00:00:00

  • Polarity of bacterial magnetotaxis is controlled by aerotaxis through a common sensory pathway.

    abstract：:Most motile bacteria navigate within gradients of external chemical stimuli by regulating the length of randomly oriented swimming episodes. Magnetotactic bacteria are characterized by chains of intracellular ferromagnetic nanoparticles and their ability to sense the geomagnetic field, which is believed to facilitate ...

    journal_title：Nature communications

    pub_type： 杂志文章

    doi：10.1038/ncomms6398

    authors： Popp F,Armitage JP,Schüler D

    更新日期：2014-11-14 00:00:00

  • A combined cryo-EM and molecular dynamics approach reveals the mechanism of ErmBL-mediated translation arrest.

    abstract：:Nascent polypeptides can induce ribosome stalling, regulating downstream genes. Stalling of ErmBL peptide translation in the presence of the macrolide antibiotic erythromycin leads to resistance in Streptococcus sanguis. To reveal this stalling mechanism we obtained 3.6-Å-resolution cryo-EM structures of ErmBL-stalled...

    journal_title：Nature communications

    pub_type： 杂志文章

    doi：10.1038/ncomms12026

    authors： Arenz S,Bock LV,Graf M,Innis CA,Beckmann R,Grubmüller H,Vaiana AC,Wilson DN

    更新日期：2016-07-06 00:00:00

  • A hepatic amino acid/mTOR/S6K-dependent signalling pathway modulates systemic lipid metabolism via neuronal signals.

    abstract：:Metabolism is coordinated among tissues and organs via neuronal signals. Levels of circulating amino acids (AAs), which are elevated in obesity, activate the intracellular target of rapamycin complex-1 (mTORC1)/S6kinase (S6K) pathway in the liver. Here we demonstrate that hepatic AA/mTORC1/S6K signalling modulates sys...

    journal_title：Nature communications

    pub_type： 杂志文章

    doi：10.1038/ncomms8940

    authors： Uno K,Yamada T,Ishigaki Y,Imai J,Hasegawa Y,Sawada S,Kaneko K,Ono H,Asano T,Oka Y,Katagiri H

    更新日期：2015-08-13 00:00:00

  • A rapid cosmic-ray increase in BC 3372-3371 from ancient buried tree rings in China.

    abstract：:Cosmic rays interact with the Earth's atmosphere to produce 14C, which can be absorbed by trees. Therefore, rapid increases of 14C in tree rings can be used to probe previous cosmic-ray events. By this method, three 14C rapidly increasing events have been found. Plausible causes of these events include large solar pro...

    journal_title：Nature communications

    pub_type： 杂志文章

    doi：10.1038/s41467-017-01698-8

    authors： Wang FY,Yu H,Zou YC,Dai ZG,Cheng KS

    更新日期：2017-11-14 00:00:00

  • An unexpected protein interaction promotes drug resistance in leukemia.

    abstract：:The overall survival of patients with acute myeloid leukemia (AML) is poor and identification of new disease-related therapeutic targets remains a major goal for this disease. Here we show that expression of MPP1, a PDZ-domain-containing protein, highly correlated with ABCC4 in AML, is associated with worse overall su...

    journal_title：Nature communications

    pub_type： 杂志文章

    doi：10.1038/s41467-017-01678-y

    authors： Pitre A,Ge Y,Lin W,Wang Y,Fukuda Y,Temirov J,Phillips AH,Peters JL,Fan Y,Ma J,Nourse A,Sinha C,Lin H,Kriwacki R,Downing JR,Gruber TA,Centonze VE,Naren AP,Chen T,Schuetz JD

    更新日期：2017-11-16 00:00:00

  • Controlled formation and reflection of a bright solitary matter-wave.

    abstract：:Bright solitons are non-dispersive wave solutions, arising in a diverse range of nonlinear, one-dimensional systems, including atomic Bose-Einstein condensates with attractive interactions. In reality, cold-atom experiments can only approach the idealized one-dimensional limit necessary for the realization of true sol...

    journal_title：Nature communications

    pub_type： 杂志文章

    doi：10.1038/ncomms2893

    authors： Marchant AL,Billam TP,Wiles TP,Yu MM,Gardiner SA,Cornish SL

    更新日期：2013-01-01 00:00:00

  • RIP1 autophosphorylation is promoted by mitochondrial ROS and is essential for RIP3 recruitment into necrosome.

    abstract：:Necroptosis is a type of programmed cell death with great significance in many pathological processes. Tumour necrosis factor-α(TNF), a proinflammatory cytokine, is a prototypic trigger of necroptosis. It is known that mitochondrial reactive oxygen species (ROS) promote necroptosis, and that kinase activity of recepto...

    journal_title：Nature communications

    pub_type： 杂志文章

    doi：10.1038/ncomms14329

    authors： Zhang Y,Su SS,Zhao S,Yang Z,Zhong CQ,Chen X,Cai Q,Yang ZH,Huang D,Wu R,Han J

    更新日期：2017-02-08 00:00:00

  • Direct RNA sequencing reveals m6A modifications on adenovirus RNA are necessary for efficient splicing.

    abstract：:Adenovirus is a nuclear replicating DNA virus reliant on host RNA processing machinery. Processing and metabolism of cellular RNAs can be regulated by METTL3, which catalyzes the addition of N6-methyladenosine (m6A) to mRNAs. While m6A-modified adenoviral RNAs have been previously detected, the location and function o...

    journal_title：Nature communications

    pub_type： 杂志文章

    doi：10.1038/s41467-020-19787-6

    authors： Price AM,Hayer KE,McIntyre ABR,Gokhale NS,Abebe JS,Della Fera AN,Mason CE,Horner SM,Wilson AC,Depledge DP,Weitzman MD

    更新日期：2020-11-26 00:00:00

  • Sex-associated molecular differences for cancer immunotherapy.

    abstract：:Immune checkpoint blockade therapies have extended patient survival across multiple cancer lineages, but there is a heated debate on whether cancer immunotherapy efficacy is different between male and female patients. We summarize the existing meta-analysis to show inconsistent conclusions for whether gender is associ...

    journal_title：Nature communications

    pub_type： 杂志文章

    doi：10.1038/s41467-020-15679-x

    authors： Ye Y,Jing Y,Li L,Mills GB,Diao L,Liu H,Han L

    更新日期：2020-04-14 00:00:00

  • Mitigating oxygen loss to improve the cycling performance of high capacity cation-disordered cathode materials.

    abstract：:Recent progress in the understanding of percolation theory points to cation-disordered lithium-excess transition metal oxides as high-capacity lithium-ion cathode materials. Nevertheless, the oxygen redox processes required for these materials to deliver high capacity can trigger oxygen loss, which leads to the format...

    journal_title：Nature communications

    pub_type： 杂志文章

    doi：10.1038/s41467-017-01115-0

    authors： Lee J,Papp JK,Clément RJ,Sallis S,Kwon DH,Shi T,Yang W,McCloskey BD,Ceder G

    更新日期：2017-10-17 00:00:00

  • Activation of G-protein-coupled receptors correlates with the formation of a continuous internal water pathway.

    abstract：:Recent crystal structures of G-protein-coupled receptors (GPCRs) have revealed ordered internal water molecules, raising questions about the functional role of those waters for receptor activation that could not be answered by the static structures. Here, we used molecular dynamics simulations to monitor--at atomic an...

    journal_title：Nature communications

    pub_type： 杂志文章

    doi：10.1038/ncomms5733

    authors： Yuan S,Filipek S,Palczewski K,Vogel H

    更新日期：2014-09-09 00:00:00

  • An investigation of irreproducibility in maximum likelihood phylogenetic inference.

    abstract：:Phylogenetic trees are essential for studying biology, but their reproducibility under identical parameter settings remains unexplored. Here, we find that 3515 (18.11%) IQ-TREE-inferred and 1813 (9.34%) RAxML-NG-inferred maximum likelihood (ML) gene trees are topologically irreproducible when executing two replicates ...

    journal_title：Nature communications

    pub_type： 杂志文章

    doi：10.1038/s41467-020-20005-6

    authors： Shen XX,Li Y,Hittinger CT,Chen XX,Rokas A

    更新日期：2020-11-30 00:00:00

  • Balanced activity in basal ganglia projection pathways is critical for contraversive movements.

    abstract：:The basal ganglia, and the striatum in particular, have been implicated in the generation of contraversive movements. The striatum projects to downstream basal ganglia nuclei through two main circuits, originating in striatonigral and striatopallidal neurons, and different models postulate that the two pathways can wo...

    journal_title：Nature communications

    pub_type： 杂志文章

    doi：10.1038/ncomms5315

    authors： Tecuapetla F,Matias S,Dugue GP,Mainen ZF,Costa RM

    更新日期：2014-07-08 00:00:00

  • Evidence for a palaeo-subglacial lake on the Antarctic continental shelf.

    abstract：:Subglacial lakes are widespread beneath the Antarctic Ice Sheet but their control on ice-sheet dynamics and their ability to harbour life remain poorly characterized. Here we present evidence for a palaeo-subglacial lake on the Antarctic continental shelf. A distinct sediment facies recovered from a bedrock basin in P...

    journal_title：Nature communications

    pub_type： 杂志文章

    doi：10.1038/ncomms15591

    authors： Kuhn G,Hillenbrand CD,Kasten S,Smith JA,Nitsche FO,Frederichs T,Wiers S,Ehrmann W,Klages JP,Mogollón JM

    更新日期：2017-06-01 00:00:00

  • Coral-associated bacteria demonstrate phylosymbiosis and cophylogeny.

    abstract：:Scleractinian corals' microbial symbionts influence host health, yet how coral microbiomes assembled over evolution is not well understood. We survey bacterial and archaeal communities in phylogenetically diverse Australian corals representing more than 425 million years of diversification. We show that coral microbio...

    journal_title：Nature communications

    pub_type： 杂志文章

    doi：10.1038/s41467-018-07275-x

    authors： Pollock FJ,McMinds R,Smith S,Bourne DG,Willis BL,Medina M,Thurber RV,Zaneveld JR

    更新日期：2018-11-22 00:00:00

  • Structural basis of Q-dependent transcription antitermination.

    abstract：:Bacteriophage Q protein engages σ-dependent paused RNA polymerase (RNAP) by binding to a DNA site embedded in late gene promoter and renders RNAP resistant to termination signals. Here, we report a single-particle cryo-electron microscopy (cryo-EM) structure of an intact Q-engaged arrested complex. The structure revea...

    journal_title：Nature communications

    pub_type： 杂志文章

    doi：10.1038/s41467-019-10958-8

    authors： Shi J,Gao X,Tian T,Yu Z,Gao B,Wen A,You L,Chang S,Zhang X,Zhang Y,Feng Y

    更新日期：2019-07-02 00:00:00

  • SMURF2 regulates bone homeostasis by disrupting SMAD3 interaction with vitamin D receptor in osteoblasts.

    abstract：:Coordination between osteoblasts and osteoclasts is required for bone health and homeostasis. Here we show that mice deficient in SMURF2 have severe osteoporosis in vivo. This low bone mass phenotype is accompanied by a pronounced increase in osteoclast numbers, although Smurf2-deficient osteoclasts have no intrinsic ...

    journal_title：Nature communications

    pub_type： 杂志文章

    doi：10.1038/ncomms14570

    authors： Xu Z,Greenblatt MB,Yan G,Feng H,Sun J,Lotinun S,Brady N,Baron R,Glimcher LH,Zou W

    更新日期：2017-02-20 00:00:00

  • High-sensitivity acoustic sensors from nanofibre webs.

    abstract：:Considerable interest has been devoted to converting mechanical energy into electricity using polymer nanofibres. In particular, piezoelectric nanofibres produced by electrospinning have shown remarkable mechanical energy-to-electricity conversion ability. However, there is little data for the acoustic-to-electric con...

    journal_title：Nature communications

    pub_type： 杂志文章

    doi：10.1038/ncomms11108

    authors： Lang C,Fang J,Shao H,Ding X,Lin T

    更新日期：2016-03-23 00:00:00