当前位置： SCI文献检索 > Nature Communications期刊下所有文献 > Highly multiplexed imaging of single cells using a high-throughput cyclic immunofluorescence method.

Highly multiplexed imaging of single cells using a high-throughput cyclic immunofluorescence method.

Abstract：

:Single-cell analysis reveals aspects of cellular physiology not evident from population-based studies, particularly in the case of highly multiplexed methods such as mass cytometry (CyTOF) able to correlate the levels of multiple signalling, differentiation and cell fate markers. Immunofluorescence (IF) microscopy adds information on cell morphology and the microenvironment that are not obtained using flow-based techniques, but the multiplicity of conventional IF is limited. This has motivated development of imaging methods that require specialized instrumentation, exotic reagents or proprietary protocols that are difficult to reproduce in most laboratories. Here we report a public-domain method for achieving high multiplicity single-cell IF using cyclic immunofluorescence (CycIF), a simple and versatile procedure in which four-colour staining alternates with chemical inactivation of fluorophores to progressively build a multichannel image. Because CycIF uses standard reagents and instrumentation and is no more expensive than conventional IF, it is suitable for high-throughput assays and screening applications.

journal_name

Nat Commun

journal_title

Nature communications

authors

Lin JR,Fallahi-Sichani M,Sorger PK

doi

10.1038/ncomms9390

subject

Has Abstract

pub_date

2015-09-24 00:00:00

pages

8390

issn

2041-1723

pii

ncomms9390

journal_volume

6

pub_type

杂志文章

相关文献

Nature Communications文献大全
  • Augmented manipulation ability in humans with six-fingered hands.

    abstract：:Neurotechnology attempts to develop supernumerary limbs, but can the human brain deal with the complexity to control an extra limb and yield advantages from it? Here, we analyzed the neuromechanics and manipulation abilities of two polydactyly subjects who each possess six fingers on their hands. Anatomical MRI of the...

    journal_title：Nature communications

    pub_type： 杂志文章

    doi：10.1038/s41467-019-10306-w

    authors： Mehring C,Akselrod M,Bashford L,Mace M,Choi H,Blüher M,Buschhoff AS,Pistohl T,Salomon R,Cheah A,Blanke O,Serino A,Burdet E

    更新日期：2019-06-03 00:00:00

  • Injection and controlled motion of conducting domain walls in improper ferroelectric Cu-Cl boracite.

    abstract：:Ferroelectric domain walls constitute a completely new class of sheet-like functional material. Moreover, since domain walls are generally writable, erasable and mobile, they could be useful in functionally agile devices: for example, creating and moving conducting walls could make or break electrical connections in n...

    journal_title：Nature communications

    pub_type： 杂志文章

    doi：10.1038/ncomms15105

    authors： McQuaid RGP,Campbell MP,Whatmore RW,Kumar A,Gregg JM

    更新日期：2017-05-16 00:00:00

  • Origin and structure of polar domains in doped molecular crystals.

    abstract：:Doping is a primary tool for the modification of the properties of materials. Occlusion of guest molecules in crystals generally reduces their symmetry by the creation of polar domains, which engender polarization and pyroelectricity in the doped crystals. Here we describe a molecular-level determination of the struct...

    journal_title：Nature communications

    pub_type： 杂志文章

    doi：10.1038/ncomms13351

    authors： Meirzadeh E,Azuri I,Qi Y,Ehre D,Rappe AM,Lahav M,Kronik L,Lubomirsky I

    更新日期：2016-11-08 00:00:00

  • Author Correction: True equilibrium measurement of transcription factor-DNA binding affinities using automated polarization microscopy.

    abstract：:In the original version of this Article, equation three contained a sign error whereby the term RT was added when it should have been subtracted. This has now been corrected in the PDF and HTML versions of the Article. ...

    journal_title：Nature communications

    pub_type： 杂志文章,已发布勘误

    doi：10.1038/s41467-019-08556-9

    authors： Jung C,Bandilla P,von Reutern M,Schnepf M,Rieder S,Unnerstall U,Gaul U

    更新日期：2019-02-05 00:00:00

  • Quantitative modeling predicts mechanistic links between pre-treatment microbiome composition and metronidazole efficacy in bacterial vaginosis.

    abstract：:Bacterial vaginosis is a condition associated with adverse reproductive outcomes and characterized by a shift from a Lactobacillus-dominant vaginal microbiota to a polymicrobial microbiota, consistently colonized by strains of Gardnerella vaginalis. Metronidazole is the first-line treatment; however, treatment failure...

    journal_title：Nature communications

    pub_type： 杂志文章

    doi：10.1038/s41467-020-19880-w

    authors： Lee CY,Cheu RK,Lemke MM,Gustin AT,France MT,Hampel B,Thurman AR,Doncel GF,Ravel J,Klatt NR,Arnold KB

    更新日期：2020-12-01 00:00:00

  • High-throughput cell and spheroid mechanics in virtual fluidic channels.

    abstract：:Microfluidics by soft lithography has proven to be of key importance for biophysics and life science research. While being based on replicating structures of a master mold using benchtop devices, design modifications are time consuming and require sophisticated cleanroom equipment. Here, we introduce virtual fluidic c...

    journal_title：Nature communications

    pub_type： 杂志文章

    doi：10.1038/s41467-020-15813-9

    authors： Panhwar MH,Czerwinski F,Dabbiru VAS,Komaragiri Y,Fregin B,Biedenweg D,Nestler P,Pires RH,Otto O

    更新日期：2020-05-04 00:00:00

  • Surface cooling caused by rare but intense near-inertial wave induced mixing in the tropical Atlantic.

    abstract：:The direct response of the tropical mixed layer to near-inertial waves (NIWs) has only rarely been observed. Here, we present upper-ocean turbulence data that provide evidence for a strongly elevated vertical diffusive heat flux across the base of the mixed layer in the presence of a NIW, thereby cooling the mixed lay...

    journal_title：Nature communications

    pub_type： 杂志文章

    doi：10.1038/s41467-020-17601-x

    authors： Hummels R,Dengler M,Rath W,Foltz GR,Schütte F,Fischer T,Brandt P

    更新日期：2020-07-31 00:00:00

  • Microring resonator-assisted Fourier transform spectrometer with enhanced resolution and large bandwidth in single chip solution.

    abstract：:Single chip integrated spectrometers are critical to bring chemical and biological sensing, spectroscopy, and spectral imaging into robust, compact and cost-effective devices. Existing on-chip spectrometer approaches fail to realize both high resolution and broad band. Here we demonstrate a microring resonator-assiste...

    journal_title：Nature communications

    pub_type： 杂志文章

    doi：10.1038/s41467-019-10282-1

    authors： Zheng SN,Zou J,Cai H,Song JF,Chin LK,Liu PY,Lin ZP,Kwong DL,Liu AQ

    更新日期：2019-05-28 00:00:00

  • Electropolymerization on wireless electrodes towards conducting polymer microfibre networks.

    abstract：:Conducting polymers can be easily obtained by electrochemical oxidation of aromatic monomers on an electrode surface as a film state. To prepare conducting polymer fibres by electropolymerization, templates such as porous membranes are necessary in the conventional methods. Here we report the electropolymerization of ...

    journal_title：Nature communications

    pub_type： 杂志文章

    doi：10.1038/ncomms10404

    authors： Koizumi Y,Shida N,Ohira M,Nishiyama H,Tomita I,Inagi S

    更新日期：2016-01-25 00:00:00

  • GPCR-specific autoantibody signatures are associated with physiological and pathological immune homeostasis.

    abstract：:Autoantibodies have been associated with autoimmune diseases. However, studies have identified autoantibodies in healthy donors (HD) who do not develop autoimmune disorders. Here we provide evidence of a network of immunoglobulin G (IgG) autoantibodies targeting G protein-coupled receptors (GPCR) in HD compared to pat...

    journal_title：Nature communications

    pub_type： 杂志文章

    doi：10.1038/s41467-018-07598-9

    authors： Cabral-Marques O,Marques A,Giil LM,De Vito R,Rademacher J,Günther J,Lange T,Humrich JY,Klapa S,Schinke S,Schimke LF,Marschner G,Pitann S,Adler S,Dechend R,Müller DN,Braicu I,Sehouli J,Schulze-Forster K,Trippel T,S

    更新日期：2018-12-06 00:00:00

  • Synthesis of acetic acid via methanol hydrocarboxylation with CO2 and H2.

    abstract：:Acetic acid is an important bulk chemical that is currently produced via methanol carbonylation using fossil based CO. Synthesis of acetic acid from the renewable and cheap CO2 is of great importance, but state of the art routes encounter difficulties, especially in reaction selectivity and activity. Here we report a ...

    journal_title：Nature communications

    pub_type： 杂志文章

    doi：10.1038/ncomms11481

    authors： Qian Q,Zhang J,Cui M,Han B

    更新日期：2016-05-11 00:00:00

  • Marangoni-driven flower-like patterning of an evaporating drop spreading on a liquid substrate.

    abstract：:Drop motility at liquid surfaces is attracting growing interest because of its potential applications in microfluidics and artificial cell design. Here we report the unique highly ordered pattern that sets in when a millimeter-size drop of dichloromethane spreads on an aqueous substrate under the influence of surface ...

    journal_title：Nature communications

    pub_type： 杂志文章

    doi：10.1038/s41467-018-03201-3

    authors： Wodlei F,Sebilleau J,Magnaudet J,Pimienta V

    更新日期：2018-02-26 00:00:00

  • MicroRNA regulation of endothelial TREX1 reprograms the tumour microenvironment.

    abstract：:Rather than targeting tumour cells directly, elements of the tumour microenvironment can be modulated to sensitize tumours to the effects of therapy. Here we report a unique mechanism by which ectopic microRNA-103 can manipulate tumour-associated endothelial cells to enhance tumour cell death. Using gain-and-loss of f...

    journal_title：Nature communications

    pub_type： 杂志文章

    doi：10.1038/ncomms13597

    authors： Wilson R,Espinosa-Diez C,Kanner N,Chatterjee N,Ruhl R,Hipfinger C,Advani SJ,Li J,Khan OF,Franovic A,Weis SM,Kumar S,Coussens LM,Anderson DG,Chen CC,Cheresh DA,Anand S

    更新日期：2016-11-25 00:00:00

  • Estimating sources and sinks of malaria parasites in Madagascar.

    abstract：:In areas where malaria epidemiology is spatially and temporally heterogeneous, human-mediated parasite importation can result in non-locally acquired clinical cases and outbreaks in low-transmission areas. Using mobility estimates derived from the mobile phone data and spatial malaria prevalence data, we identify trav...

    journal_title：Nature communications

    pub_type： 杂志文章

    doi：10.1038/s41467-018-06290-2

    authors： Ihantamalala FA,Herbreteau V,Rakotoarimanana FMJ,Rakotondramanga JM,Cauchemez S,Rahoilijaona B,Pennober G,Buckee CO,Rogier C,Metcalf CJE,Wesolowski A

    更新日期：2018-09-25 00:00:00

  • Electrically tunable optical polarization rotation on a silicon chip using Berry's phase.

    abstract：:The continued convergence of electronics and photonics on the chip scale can benefit from the voltage control of optical polarization for applications in communications, signal processing and sensing. It is challenging, however, to electrically manipulate the polarization state of light in planar optical waveguides. H...

    journal_title：Nature communications

    pub_type： 杂志文章

    doi：10.1038/ncomms6337

    authors： Xu Q,Chen L,Wood MG,Sun P,Reano RM

    更新日期：2014-11-12 00:00:00

  • Cascading ecological effects of eliminating fishery discards.

    abstract：:Discarding by fisheries is perceived as contrary to responsible harvesting. Legislation seeking to end the practice is being introduced in many jurisdictions. However, discarded fish are food for a range of scavenging species; so, ending discarding may have ecological consequences. Here we investigate the sensitivity ...

    journal_title：Nature communications

    pub_type： 杂志文章

    doi：10.1038/ncomms4893

    authors： Heath MR,Cook RM,Cameron AI,Morris DJ,Speirs DC

    更新日期：2014-05-13 00:00:00

  • Identification of DNA lesions using a third base pair for amplification and nanopore sequencing.

    abstract：:Damage to the genome is implicated in the progression of cancer and stress-induced diseases. DNA lesions exist in low levels, and cannot be amplified by standard PCR because they are frequently strong blocks to polymerases. Here, we describe a method for PCR amplification of lesion-containing DNA in which the site and...

    journal_title：Nature communications

    pub_type： 杂志文章

    doi：10.1038/ncomms9807

    authors： Riedl J,Ding Y,Fleming AM,Burrows CJ

    更新日期：2015-11-06 00:00:00

  • Bypassing stroke-damaged neural pathways via a neural interface induces targeted cortical adaptation.

    abstract：:Regaining the function of an impaired limb is highly desirable in paralyzed individuals. One possible avenue to achieve this goal is to bridge the interrupted pathway between preserved neural structures and muscles using a brain-computer interface. Here, we demonstrate that monkeys with subcortical stroke were able to...

    journal_title：Nature communications

    pub_type： 杂志文章

    doi：10.1038/s41467-019-12647-y

    authors： Kato K,Sawada M,Nishimura Y

    更新日期：2019-10-16 00:00:00

  • Fast and accurate sCMOS noise correction for fluorescence microscopy.

    abstract：:The rapid development of scientific CMOS (sCMOS) technology has greatly advanced optical microscopy for biomedical research with superior sensitivity, resolution, field-of-view, and frame rates. However, for sCMOS sensors, the parallel charge-voltage conversion and different responsivity at each pixel induces extra re...

    journal_title：Nature communications

    pub_type： 杂志文章

    doi：10.1038/s41467-019-13841-8

    authors： Mandracchia B,Hua X,Guo C,Son J,Urner T,Jia S

    更新日期：2020-01-03 00:00:00

  • Artificial morphogen-mediated differentiation in synthetic protocells.

    abstract：:The design and assembly of artificial protocell consortia displaying dynamical behaviours and systems-based properties are emerging challenges in bottom-up synthetic biology. Cellular processes such as morphogenesis and differentiation rely in part on reaction-diffusion gradients, and the ability to mimic rudimentary ...

    journal_title：Nature communications

    pub_type： 杂志文章

    doi：10.1038/s41467-019-11316-4

    authors： Tian L,Li M,Patil AJ,Drinkwater BW,Mann S

    更新日期：2019-07-25 00:00:00

  • RORγt inhibition selectively targets IL-17 producing iNKT and γδ-T cells enriched in Spondyloarthritis patients.

    abstract：:Dysregulated IL-23/IL-17 responses have been linked to psoriatic arthritis and other forms of spondyloarthritides (SpA). RORγt, the key Thelper17 (Th17) cell transcriptional regulator, is also expressed by subsets of innate-like T cells, including invariant natural killer T (iNKT) and γδ-T cells, but their contributio...

    journal_title：Nature communications

    pub_type： 杂志文章

    doi：10.1038/s41467-018-07911-6

    authors： Venken K,Jacques P,Mortier C,Labadia ME,Decruy T,Coudenys J,Hoyt K,Wayne AL,Hughes R,Turner M,Van Gassen S,Martens L,Smith D,Harcken C,Wahle J,Wang CT,Verheugen E,Schryvers N,Varkas G,Cypers H,Wittoek R,Piette Y

    更新日期：2019-01-02 00:00:00

  • miR200-regulated CXCL12β promotes fibroblast heterogeneity and immunosuppression in ovarian cancers.

    abstract：:High-grade serous ovarian cancers (HGSOC) have been subdivided into molecular subtypes. The mesenchymal HGSOC subgroup, defined by stromal-related gene signatures, is invariably associated with poor patient survival. We demonstrate that stroma exerts a key function in mesenchymal HGSOC. We highlight stromal heterogene...

    journal_title：Nature communications

    pub_type： 杂志文章

    doi：10.1038/s41467-018-03348-z

    authors： Givel AM,Kieffer Y,Scholer-Dahirel A,Sirven P,Cardon M,Pelon F,Magagna I,Gentric G,Costa A,Bonneau C,Mieulet V,Vincent-Salomon A,Mechta-Grigoriou F

    更新日期：2018-03-13 00:00:00

  • Optical soliton molecular complexes in a passively mode-locked fibre laser.

    abstract：:Ultrashort optical pulses propagating in a dissipative nonlinear system can interact and bind stably, forming optical soliton molecules. Soliton molecules in ultrafast lasers are under intense research focus and present striking analogies with their matter molecules counterparts. The recent development of real-time sp...

    journal_title：Nature communications

    pub_type： 杂志文章

    doi：10.1038/s41467-019-08755-4

    authors： Wang ZQ,Nithyanandan K,Coillet A,Tchofo-Dinda P,Grelu P

    更新日期：2019-02-19 00:00:00

  • Temporally sequenced anticancer drugs overcome adaptive resistance by targeting a vulnerable chemotherapy-induced phenotypic transition.

    abstract：:Understanding the emerging models of adaptive resistance is key to overcoming cancer chemotherapy failure. Using human breast cancer explants, in vitro cell lines, mouse in vivo studies and mathematical modelling, here we show that exposure to a taxane induces phenotypic cell state transition towards a favoured transi...

    journal_title：Nature communications

    pub_type： 杂志文章

    doi：10.1038/ncomms7139

    authors： Goldman A,Majumder B,Dhawan A,Ravi S,Goldman D,Kohandel M,Majumder PK,Sengupta S

    更新日期：2015-02-11 00:00:00

  • Publisher Correction: A multistage rotational speed changing molecular rotor regulated by pH and metal cations.

    abstract：:The original version of this Article omitted the Received and Accepted dates; they should have been 22nd January 2018 and 19th April 2018, respectively. This has been corrected in the PDF and HTML versions of the Article. ...

    journal_title：Nature communications

    pub_type： 杂志文章,已发布勘误

    doi：10.1038/s41467-018-04902-5

    authors： Wu Y,Wang G,Li Q,Xiang J,Jiang H,Wang Y

    更新日期：2018-06-13 00:00:00

  • Hierarchically structured lithium titanate for ultrafast charging in long-life high capacity batteries.

    abstract：:High-performance Li-ion batteries require materials with well-designed and controlled structures on nanometre and micrometre scales. Electrochemical properties can be enhanced by reducing crystallite size and by manipulating structure and morphology. Here we show a method for preparing hierarchically structured Li4Ti5...

    journal_title：Nature communications

    pub_type： 杂志文章

    doi：10.1038/ncomms15636

    authors： Odziomek M,Chaput F,Rutkowska A,Świerczek K,Olszewska D,Sitarz M,Lerouge F,Parola S

    更新日期：2017-05-26 00:00:00

  • Turning the respiratory flexibility of Mycobacterium tuberculosis against itself.

    abstract：:The Mycobacterium tuberculosis (Mtb) electron transport chain (ETC) has received significant attention as a drug target, however its vulnerability may be affected by its flexibility in response to disruption. Here we determine the effect of the ETC inhibitors bedaquiline, Q203 and clofazimine on the Mtb ETC, and the v...

    journal_title：Nature communications

    pub_type： 杂志文章

    doi：10.1038/ncomms12393

    authors： Lamprecht DA,Finin PM,Rahman MA,Cumming BM,Russell SL,Jonnala SR,Adamson JH,Steyn AJ

    更新日期：2016-08-10 00:00:00

  • Exosome engineering for efficient intracellular delivery of soluble proteins using optically reversible protein-protein interaction module.

    abstract：:Nanoparticle-mediated delivery of functional macromolecules is a promising method for treating a variety of human diseases. Among nanoparticles, cell-derived exosomes have recently been highlighted as a new therapeutic strategy for the in vivo delivery of nucleotides and chemical drugs. Here we describe a new tool for...

    journal_title：Nature communications

    pub_type： 杂志文章

    doi：10.1038/ncomms12277

    authors： Yim N,Ryu SW,Choi K,Lee KR,Lee S,Choi H,Kim J,Shaker MR,Sun W,Park JH,Kim D,Heo WD,Choi C

    更新日期：2016-07-22 00:00:00

  • Super-resolution architecture of mammalian centriole distal appendages reveals distinct blade and matrix functional components.

    abstract：:Distal appendages (DAPs) are nanoscale, pinwheel-like structures protruding from the distal end of the centriole that mediate membrane docking during ciliogenesis, marking the cilia base around the ciliary gate. Here we determine a super-resolved multiplex of 16 centriole-distal-end components. Surprisingly, rather th...

    journal_title：Nature communications

    pub_type： 杂志文章

    doi：10.1038/s41467-018-04469-1

    authors： Yang TT,Chong WM,Wang WJ,Mazo G,Tanos B,Chen Z,Tran TMN,Chen YD,Weng RR,Huang CE,Jane WN,Tsou MB,Liao JC

    更新日期：2018-05-22 00:00:00

  • MLKL trafficking and accumulation at the plasma membrane control the kinetics and threshold for necroptosis.

    abstract：:Mixed lineage kinase domain-like (MLKL) is the terminal protein in the pro-inflammatory necroptotic cell death program. RIPK3-mediated phosphorylation is thought to initiate MLKL oligomerization, membrane translocation and membrane disruption, although the precise choreography of events is incompletely understood. Her...

    journal_title：Nature communications

    pub_type： 杂志文章

    doi：10.1038/s41467-020-16887-1

    authors： Samson AL,Zhang Y,Geoghegan ND,Gavin XJ,Davies KA,Mlodzianoski MJ,Whitehead LW,Frank D,Garnish SE,Fitzgibbon C,Hempel A,Young SN,Jacobsen AV,Cawthorne W,Petrie EJ,Faux MC,Shield-Artin K,Lalaoui N,Hildebrand JM,Silke

    更新日期：2020-06-19 00:00:00