Abstract:
ETHNOPHARMACOLOGICAL RELEVANCE:Poria cocos Wolf (P. cocos), a well-known traditional East-Asian medicinal and edible fungus, is one of the most important components in Chinese medicine formulas like "Guizhi fuling wan" to treat hyperplasia of mammary glands and breast cancer. AIMING OF STUDY:In this study, we attempted to verify the anticancer efficacy of the ethanol extract of P. cocos (PC) on the breast cancer as well as to investigate its most active compound and its underlying molecular mechanism in vivo and in vitro. MATERIALS AND METHODS:The key anti-cancer components were separated and purified through chromatography and identified by spectral analyses. The in vivo anti-breast cancer efficacy and side effects of PC were evaluated in BALB/c nude mice that have been subcutaneously injected with breast cancer cells MDA-MB-231. Cytotoxicity, apoptosis and cell cycle arrest of PC were evaluated in vitro by cell viability assays and flow cytometry. The protein levels were examined via western blotting. RESULTS:Pachymic acid (PA), separated and identified as the most active compound, induced the significant cytotoxicity on breast cancer cells MDA-MB-231(IC50 value, 2.13 ± 0.24 μg/mL) and was not active against the normal breast epithelium cells MCF-10A. The in vivo experiment revealed that PC could significantly inhibit the tumor development and the final mean tumor weight of the mice in the PC group (0.51 ± 0.12g) was significantly lower than that in the model group (1.22 ± 0.45g). Notably, compared to the first-line anticancer drug cisplatin, PC showed less side effects on the function of the vital organs and the muscle strength of the mice. Among in vitro study, PC significantly inhibited the cell growth of MDA-MB-231 by inducing cell apoptosis and cell cycle arrested at G0/G1 phase in a dose-dependent manner. The expression of cell cycle-associated cyclin D1, cyclin E, CDK2, and CDK4 were downregulated, while p53 and p21 expression were upregulated following the PA treatment. In addition, PA downregulated the apoptotic regulator Bcl-2, increased the expression of pro-apoptotic protein Bax, and promoted the release of cytochrome c and the activation of cleaved caspase-3, -9 and caspase -8 via mitochondria-mediated and death receptor-mediated signaling pathways. CONCLUSION:This study verified the anticancer efficacy of PC on breast cancer in vivo and in vitro through induction of cell apoptosis and G0/G1 cell cycle arrest. The data also suggested that PA could be developed as an efficacious agent for breast cancer treatment with less side effects.
journal_name
J Ethnopharmacoljournal_title
Journal of ethnopharmacologyauthors
Jiang Y,Fan Ldoi
10.1016/j.jep.2020.112851subject
Has Abstractpub_date
2020-07-15 00:00:00pages
112851eissn
0378-8741issn
1872-7573pii
S0378-8741(19)34820-2journal_volume
257pub_type
杂志文章abstract::Traditional Korean medicine, Cheongyeolsaseuptang (CYSST) has been widely applied as a treatment of rheumatoid arthritis (RA) in Korea. However, its effect in experimental models remains unknown. Recent reports suggest that in patients with RA, synovial mast cells increase in number and show signs of activation and pr...
journal_title:Journal of ethnopharmacology
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更新日期:2005-02-10 00:00:00
abstract::The ethanol extract of Ageratum conyzoides was evaluated for gastroprotection in rats using the Ibuprofen, ethanol and cold restraint stress ulcer models. Efficacy was assessed by determination of mean ulcer size, ulcer number and ulcer index. Oral administration of the ethanol extract at dose levels of 500 and 750 mg...
journal_title:Journal of ethnopharmacology
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journal_title:Journal of ethnopharmacology
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journal_title:Journal of ethnopharmacology
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更新日期:2018-01-30 00:00:00
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journal_title:Journal of ethnopharmacology
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更新日期:2021-01-21 00:00:00
abstract:BACKGROUND AND PURPOSE:Based on ethno-botanical information collected from diabetic patients in Cuba and firstly reported inhibition of PTP1B and DPPIV enzymes activities, Allophylus cominia (A. cominia) was identified as possible source of new drugs that could be used for the treatment of type 2 diabetes mellitus (T2-...
journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
doi:10.1016/j.jep.2018.01.014
更新日期:2018-04-24 00:00:00
abstract:ETHNOPHARMACOLOGICAL RELEVANCE:Fritillariae Bulbus (Beimu in Chinese) is derived from the bulbus of many Fritillaria species (family Liliaceae), which has been used as an antitussive herb in traditional Chinese medicine for more than 2000 years. Due to the complexity of plant origins and significant variations in chemi...
journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
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更新日期:2018-01-10 00:00:00
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journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
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journal_title:Journal of ethnopharmacology
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doi:10.1016/j.jep.2010.08.039
更新日期:2010-10-28 00:00:00
abstract::Tanzania is endowed with a rich natural resource. The biodiversity comprises over 10,000 plant species, a rich fauna and marine resources. The collection of biological resources is guided by a set of formalities, namely: (i) entry visas, (ii) research permits, (iii) designation of a relevant collaborating host institu...
journal_title:Journal of ethnopharmacology
pub_type: 杂志文章,评审
doi:10.1016/0378-8741(95)01365-2
更新日期:1996-04-01 00:00:00
abstract:ETHNOPHARMACOLOGICAL RELEVANCE:In Africa, traditional medicine encompasses a diverse range of practices, including herbalism and spiritualism, where some diseases are believed to be "African" since they can only be traditionally treated. Indigenous knowledge on the management of "African" diseases using medicinal plant...
journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
doi:10.1016/j.jep.2020.113578
更新日期:2021-03-25 00:00:00
abstract::Himatanthus lancifolius, popularly known as "agoniada" in Brazil, is largely used in folk medicine against asthma, dysmenorrhea and as an emenagogue and abortive. This study reveals the effects of an alkaloid rich fraction (AlkF) obtained from the bark of Himatanthus lancifolius in vascular and non-vascular smooth mus...
journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
doi:10.1016/j.jep.2005.02.041
更新日期:2005-09-14 00:00:00
abstract::The hepatoprotective effect of the ethanol/water (1:1) extract of Eclipta alba (Ea) has been studied at subcellular levels in rats against CCl4-induced hepatotoxicity. Ea significantly counteracted CCl4-induced inhibition of the hepatic microsomal drug metabolising enzyme amidopyrine N-demethylase and membrane bound g...
journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
doi:10.1016/0378-8741(93)90063-b
更新日期:1993-12-01 00:00:00
abstract::Mangifera indica is commonly grown in many parts of the world. Its seeds have been used for anti-diarrhoeal activity in Indian traditional medicine. This study evaluates the potential anti-diarrhoeal activity of methanolic (MMI) and aqueous (AMI) extracts of seeds of M. indica in experimental diarrhoea, induced by cas...
journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
doi:10.1016/s0378-8741(02)00250-7
更新日期:2003-01-01 00:00:00
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journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
doi:10.1016/j.jep.2012.05.041
更新日期:2012-08-01 00:00:00
abstract:ETHNOPHARMACOLOGICAL RELEVANCE:Medicinal plants are widely used for treatment of oral/dental diseases in Uganda. AIM OF THE STUDY:To investigate antibacterial activities of 16 commonly used medicinal plants on microorganisms associated with periodontal diseases (PD) and dental caries (DC). MATERIALS AND METHODS:Pulp ...
journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
doi:10.1016/j.jep.2014.06.027
更新日期:2014-08-08 00:00:00
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journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
doi:10.1016/j.jep.2020.113538
更新日期:2021-03-01 00:00:00
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journal_title:Journal of ethnopharmacology
pub_type: 杂志文章,评审
doi:10.1016/j.jep.2014.10.014
更新日期:2014-12-02 00:00:00
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journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
doi:10.1016/s0378-8741(99)00011-2
更新日期:1999-11-01 00:00:00
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journal_title:Journal of ethnopharmacology
pub_type: 杂志文章,评审
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更新日期:2019-03-25 00:00:00
abstract::It has previously been shown that EtOAc extracts of Rhus verniciflua Stokes (RVS) inhibit the platelet aggregation response. In this report, bioassay-guided fractionation using ADP-, arachidonic acid-, and collagen-induced human platelet aggregation by a whole blood aggregometer yielded the bioactive compounds isomalt...
journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
doi:10.1016/j.jep.2005.12.015
更新日期:2006-06-15 00:00:00
abstract:ETHNOPHARMACOLOGICAL RELEVANCE:Smilax glabra rhizome, a plant material from Liliaceae family, is a widely used traditional Chinese medicine for anti-cardiac hypertrophy treatment. We have previously found that Smilax glabra flavonoids (SGF) exerted such anti-cardiac hypertrophy activity. However, the mechanism of this ...
journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
doi:10.1016/j.jep.2013.08.009
更新日期:2013-10-28 00:00:00
abstract::The Taylor-Schechter (T-S) collection at Cambridge University Library is the biggest of all Cairo Genizah collections in the world. The importance and the potential of research into the medical aspects of the Genizah documents were clear to researcher since the early 1960s. A few works have been published since, usual...
journal_title:Journal of ethnopharmacology
pub_type: 历史文章,杂志文章
doi:10.1016/j.jep.2006.06.005
更新日期:2006-12-06 00:00:00
abstract::Essential oils of seven Moroccan Labiatae were chemically analysed by GC-MS and evaluated for their in vitro antifungal activity against Botrytis cinerea. Among them, Origanum compactum and Thymus glandulosus greatly inhibited the growth of the mycelium. The inhibition of Botrytis cinerea was 100% for both oils at 100...
journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
doi:10.1016/s0378-8741(03)00275-7
更新日期:2003-11-01 00:00:00
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journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
doi:10.1016/s0378-8741(96)01500-0
更新日期:1997-02-01 00:00:00
abstract:ETHNOPHARMACOLOGICAL RELEVANCE:Melicope lunu-ankenda leaves are used to treat diabetes in folklore medicinal practices in India and Malaysia. Here we report the isolation of an O-prenylated flavonoid (3,5,4'-trihydroxy-8,3'-dimethoxy-7-(3-methylbut-2-enoxy)flavone; OPF) from the leaves of M. lunu-ankenda and its antidi...
journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
doi:10.1016/j.jep.2015.03.060
更新日期:2015-06-20 00:00:00
abstract:AIM:CGX, a modified traditional Chinese herbal drug whose name means "liver cleaning," is used to treat various liver disorders. This study investigated the protective effects of CGX and its mechanisms. MATERIAL AND METHODS:After pretreating ICR mice twice daily with CGX (po, 50 or 100mg/kg) or distilled water for thr...
journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
doi:10.1016/j.jep.2008.07.042
更新日期:2008-10-30 00:00:00
abstract:AIM OF THE STUDY:To investigate the hypoglycemic effects of Vaccinium bracteatum Thunb. leaves (VBTL) extract in streptozotocin-induced diabetic mice. MATERIALS AND METHODS:After administration of VBTL extract for 4 weeks, the body weight, organ weight, blood glucose (BG), insulin and plasma lipid levels of streptozot...
journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
doi:10.1016/j.jep.2010.05.031
更新日期:2010-08-09 00:00:00
abstract:ETHNOPHARMACOLOGICAL RELEVANCE:Aristolochia ringens Vahl. (Aristolochiaceae) is used traditionally in Nigeria for managing a number of ailments including gastrointestinal disturbances, rheumatoid arthritis, pile, insomnia, oedema, and snake bite venom. Some studies in our laboratory have demonstrated a scientific justi...
journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
doi:10.1016/j.jep.2019.112150
更新日期:2019-11-15 00:00:00
abstract::In this study, the effect of regular khat chewing on serum glucose and C-peptide levels were investigated in both healthy male individuals and type 2 diabetic patients. The results presented show the levels of glucose and C-peptide of healthy individuals to be non-significantly different between khat and non-khat chew...
journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
doi:10.1016/s0378-8741(03)00035-7
更新日期:2003-05-01 00:00:00