Naringin inhibits autophagy mediated by PI3K-Akt-mTOR pathway to ameliorate endothelial cell dysfunction induced by high glucose/high fat stress.


:As a flavonoid, naringin (Nar) has been shown to have multiple pharmacological effects including lowering blood cholesterol, reducing thrombus formation and improving microcirculation. However, effects of Nar on function and autophagy of vascular endothelial cells under high glucose and high fat (HG/HF) stress are largely unclear. This study was designed to investigate such effects of Nar in human umbilical vein endothelial cells (HUVECs) and to determine whether such effects are related to autophagy. Our present results show that 86 μM of Nar inhibits the autophagy levels and protects the cells against the dysfunction induced by HG/HF stress. Moreover, Nar increases the phosphorylation levels of phosphatidylinositol-3-kinase (PI3K), protein kinase B (Akt) and mammalian rapamycin target protein (mTOR). However, pretreatment with rapamycin (RAPA, 5 μM, autophagy inducer), LY294002(10 μM, PI3K inhibitor) and Akt inhibitor Ⅳ (0.5 μM, Akt inhibitor) partially abrogates the protective effects of Nar, suggesting that the protective effects of Nar are achieved by activating the PI3K-Akt-mTOR pathway to inhibit autophagy. In conclusion, Nar improves the function of HUVECs under HG/HF stress through activating the PI3K-Akt-mTOR pathway to inhibit autophagy. The findings offer an insight into HG/HF stress-induced autophagy and indicate that Nar might have potential to prevent and treat the diabetic angiopathy.


Eur J Pharmacol


Wang K,Peng S,Xiong S,Niu A,Xia M,Xiong X,Zeng G,Huang Q




Has Abstract


2020-05-05 00:00:00












  • Naturally occurring antioxidant nutrients reduce inflammatory response in mice.

    abstract::The effects of mixed dietary coenzyme Q(9), alpha-tocopherol, and beta-carotene on immune cell activity and blood cytokine profile were studied in peritoneal macrophages, spleen lymphocytes, and blood plasma from mice with acute inflammation induced by lipopolysaccharide (LPS). The activity of each fat-soluble antioxi...

    journal_title:European journal of pharmacology

    pub_type: 杂志文章


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    更新日期:2009-08-01 00:00:00

  • Galangin inhibits proliferation of HepG2 cells by activating AMPK via increasing the AMP/TAN ratio in a LKB1-independent manner.

    abstract::Galangin, a flavonol derived from Alpinia officinarum Hance and used as food additives in southern China, induces apoptosis and autophagy to suppress the proliferation of HepG2 cells. In this study, we demonstrated that galangin induced autophagy by increasing the ratio of AMP/TAN in HepG2 cells. It stimulated the pho...

    journal_title:European journal of pharmacology

    pub_type: 杂志文章


    authors: Zhang H,Li N,Wu J,Su L,Chen X,Lin B,Luo H

    更新日期:2013-10-15 00:00:00

  • Benidipine, an anti-hypertensive drug, inhibits reactive oxygen species production in polymorphonuclear leukocytes and oxidative stress in salt-loaded stroke-prone spontaneously hypertensive rats.

    abstract::Oxidative stress is associated with exacerbation of renal injuries in hypertension. In clinical studies benidipine hydrochloride (benidipine), a dihydropyridine calcium channel blocker with antioxidant activity, reduced oxidative stress. However, the mechanism of suppression of oxidative stress remains to be fully cha...

    journal_title:European journal of pharmacology

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  • Reduced contractile function after balloon denudation of rat carotid arteries.

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    journal_title:European journal of pharmacology

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    更新日期:1994-04-11 00:00:00

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    journal_title:European journal of pharmacology

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  • Inhibition of platelet aggregation by capsaicin. An effect unrelated to actions on sensory afferent neurons.

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    journal_title:European journal of pharmacology

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  • LncRNA OR3A4 participates in the angiogenesis of hepatocellular carcinoma through modulating AGGF1/akt/mTOR pathway.

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    journal_title:European journal of pharmacology

    pub_type: 杂志文章


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  • Imaging of beta-adrenoceptors in the human thorax using (S)-[(11)C]CGP12388 and positron emission tomography.

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    journal_title:European journal of pharmacology

    pub_type: 杂志文章


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  • The selective 5-HT(6) receptor antagonist SB-399885 enhances anti-immobility action of antidepressants in rats.

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  • Effect of uncouplers of oxidative phosphorylation on transmitter release in dystrophic mice.

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    pub_type: 杂志文章


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    journal_title:European journal of pharmacology

    pub_type: 杂志文章


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  • Mouse models rarely mimic the transcriptome of human neurodegenerative diseases: A systematic bioinformatics-based critique of preclinical models.

    abstract::Translational research for neurodegenerative disease depends intimately upon animal models. Unfortunately, promising therapies developed using mouse models mostly fail in clinical trials, highlighting uncertainty about how well mouse models mimic human neurodegenerative disease at the molecular level. We compared the ...

    journal_title:European journal of pharmacology

    pub_type: 杂志文章


    authors: Burns TC,Li MD,Mehta S,Awad AJ,Morgan AA

    更新日期:2015-07-15 00:00:00

  • In vivo effects of some tertiary alkylaminoethyl esters with choline acetyltransferase inhibitory properties.

    abstract::The tertiary nitrogen derivatives of two choline esters, chloroacetylcholine and acrylcholine, known to inhibit choline acetyltransferase (ChAc) in vitro, were tested for their effects in the whole animal, including peripheral and central cholinergic systems. These esters are N,N-dimethylaminoethyl chloroacetate (Cl-D...

    journal_title:European journal of pharmacology

    pub_type: 杂志文章


    authors: Rowell PP,Chiou CY

    更新日期:1976-11-01 00:00:00

  • Anandamide enhances expression of heat shock proteins Hsp70 and Hsp25 in rat lungs.

    abstract::Anandamide (AEA), an endogenous cannabinoid and vanilloid receptor ligand, possesses anti-inflammatory properties. Transport of AEA through cytoplasm is facilitated by heat shock protein (HSP) Hsp70, which enhances the rate of cellular AEA uptake, possibly via direct interactions. In lungs, increased HSP expression is...

    journal_title:European journal of pharmacology

    pub_type: 杂志文章


    authors: Kopczyńska B,Sulejczak D,Wełniak-Kamińska M,Gietka A,Grieb P

    更新日期:2011-10-01 00:00:00

  • Effect of almitrine on upper airway muscle contraction in young and old rats.

    abstract::The effects of almitrine on the contractile properties of isolated geniohyoid and sternohyoid muscles were determined in physiological salt solution at 30 degrees C in young and old rats. In young rats, almitrine had no effect on twitch or tetanic tension, twitch:tetanic tension ratio, contractile kinetics, active or ...

    journal_title:European journal of pharmacology

    pub_type: 杂志文章


    authors: Cantillon D,Bradford A

    更新日期:2001-01-26 00:00:00

  • Morroniside enhances angiogenesis and improves cardiac function following acute myocardial infarction in rats.

    abstract::Angiogenesis is critical for re-establishing blood supply to the ischemic myocardium after acute myocardial infarction (AMI). This study aimed to investigate the effects of morroniside on angiogenesis after AMI and explored associated proangiogenic mechanisms. A rat model of AMI was established by ligation of the left...

    journal_title:European journal of pharmacology

    pub_type: 杂志文章


    authors: Liu T,Sun F,Cui J,Zheng S,Li Z,Guo D,Tian X,Zhu Z,Zheng W,Wang Y,Wang W

    更新日期:2020-04-05 00:00:00

  • Novel iboga alkaloid congeners block nicotinic receptors and reduce drug self-administration.

    abstract::18-Methoxycoronaridine, a novel iboga alkaloid congener, reduces drug self-administration in animal models of addiction. Previously, we proposed that these effects are mediated by the ability of 18-methoxycoronaridine to inhibit nicotinic alpha3beta4 acetylcholine receptors. In an attempt to identify more potent 18-me...

    journal_title:European journal of pharmacology

    pub_type: 杂志文章


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    更新日期:2004-05-25 00:00:00

  • Hepatoprotective effect of vitamin C on lithocholic acid-induced cholestatic liver injury in Gulo(-/-) mice.

    abstract::Prevention and restoration of hepatic fibrosis from chronic liver injury is essential for the treatment of patients with chronic liver diseases. Vitamin C is known to have hepatoprotective effects, but their underlying mechanisms are unclear, especially those associated with hepatic fibrosis. Here, we analyzed the imp...

    journal_title:European journal of pharmacology

    pub_type: 杂志文章


    authors: Yu SJ,Bae S,Kang JS,Yoon JH,Cho EJ,Lee JH,Kim YJ,Lee WJ,Kim CY,Lee HS

    更新日期:2015-09-05 00:00:00

  • Enhanced vasodilatory responses to bradykinin in stroke-prone spontaneously hypertensive rats.

    abstract::Vasodilatory responses to bradykinin (BK) and acetylcholine (ACh) were compared in phenylephrine preconstricted perfused kidneys from 30-week-old male stroke-prone spontaneously hypertensive rats (SHRSP) and normotensive Wistar-Kyoto rats (WKY). Responses to ACh did not differ between kidneys from SHRSP and WHY. BK-in...

    journal_title:European journal of pharmacology

    pub_type: 杂志文章


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    更新日期:1992-01-14 00:00:00

  • Fisetin, via CKIP-1/REGγ, limits oxidized LDL-induced lipid accumulation and senescence in RAW264.7 macrophage-derived foam cells.

    abstract::To test the hypothesis that the flavonoid compound, fisetin, protects macrophages from lipid accumulation and senescence through regulation of casein kinase 2-interacting protein-1 (CKIP-1)/REGγ (11S regulatory particles, 28 kDa proteasome activator, proteasome activator subunit 3) signaling. RAW264.7 macrophage cells...

    journal_title:European journal of pharmacology

    pub_type: 杂志文章


    authors: Jia Q,Cao H,Shen D,Yan L,Chen C,Xing S

    更新日期:2019-12-15 00:00:00

  • Prostaglandin E2 receptor EP3 subtype in the paraventricular hypothalamic nucleus mediates corticotropin-releasing factor-induced elevation of plasma noradrenaline levels in rats.

    abstract::Corticotropin-releasing factor (CRF) plays an important role in sympathetic regulation. Centrally administered CRF elevates plasma catecholamine levels, resulting in CRF-dependent hypertension and tachycardia. We previously reported that brain thromboxane A2 mediates CRF-induced elevation of plasma adrenaline levels, ...

    journal_title:European journal of pharmacology

    pub_type: 杂志文章


    authors: Yamaguchi N,Mimura K,Okada S

    更新日期:2019-11-15 00:00:00