Abstract:
:The present study aimed at evaluating the role of senescent cell microenvironment as an extrinsic causal factor for altered age-associated macrophage functions, and that whether such changes could be ameliorated by the application of tea catechin epigallocatechin gallate (EGCG). To ascertain this, we analyzed the impact of secretory metabolites of proliferating (P) and senescent (S) preadipocyte cells on the induction of phenotypic and functional characteristics associated with aging in macrophages isolated from young (YM) and old (OM) C57BL/6J mice. The role of EGCG as alleviator of preadipocyte media-induced senescence and inflamm-aging was evaluated in OM. Results revealed strong age-related dysregulation in macrophage functions as evident by decreased CD11b expression, enhanced expression of cytokines (IL-6/TNF-α/IL-1β/IL-10) and cell cycle inhibitors p53/p21WAF1/p16Ink4a, as well as augmentation of M2 phenotype (Arg1/Msr1/Mrc1) and SA-β-gal activity. Ex vivo exposure of macrophages (YM and OM) to secretory factors of preadipocytes induced differential effects, and treatment with S culture media largely showed an augmentation of senescent phenotype, particularly in the YM. Pretreatment with EGCG (10 µM) to OM caused a dramatic reversal of both age-associated and preadipocyte media-induced changes as evident from upregulation of CD11b and ROS levels, inhibition of inflammatory makers, attenuation of p53/p21WAF1/p16Ink4a expression and SA-β-gal activity. Our results indicate vital role of adipose tissue-mediated extrinsic factors in shaping macrophage phenotype and functions during aging. It is also apparent that EGCG is a promising candidate in developing preventive therapies aimed at alleviating macrophage inflamm-aging and senescence that may help curb incidences of inflammatory disorders in elderly.
journal_name
Biogerontologyjournal_title
Biogerontologyauthors
Kumar R,Sharma A,Padwad Y,Sharma Rdoi
10.1007/s10522-020-09861-3subject
Has Abstractpub_date
2020-06-01 00:00:00pages
325-343issue
3eissn
1389-5729issn
1573-6768pii
10.1007/s10522-020-09861-3journal_volume
21pub_type
杂志文章相关文献
BIOGERONTOLOGY文献大全abstract::Senescence-associated secretory phenotype (SASP) plays a role in aging adipose tissue dysfunction by directly promoting chronic inflammation. The JNK/p53 pathway was reported as a potential mechanism that mediates SASP. In this study, we investigated the effects of L-carnitine, an inhibitor of the JNK/p53 pathway in a...
journal_title:Biogerontology
pub_type: 杂志文章
doi:10.1007/s10522-018-9787-z
更新日期:2019-04-01 00:00:00
abstract::CD4+ T cells are important for generating high quality and robust immune responses to influenza infection. Immunosenescence that occurs with aging, however, compromises the ability of CD4+ T cells to differentiate into functional subsets resulting in a multitude of dysregulated responses namely, delayed viral clearanc...
journal_title:Biogerontology
pub_type: 杂志文章,评审
doi:10.1007/s10522-018-9754-8
更新日期:2018-12-01 00:00:00
abstract::High-throughput molecular studies are greatly advancing our knowledge of the human microbiome and its specific role in governing health and disease states. A myriad of ongoing studies aim at identifying links between microbial community disequilibria (dysbiosis) and human diseases. However, due to the inherent complex...
journal_title:Biogerontology
pub_type: 杂志文章
doi:10.1007/s10522-018-9752-x
更新日期:2018-12-01 00:00:00
abstract::The senescence-accelerated mouse (SAM) prone 8 (SAMP8) has been demonstrated for muscular aging research including sarcopenia, but its underlying mechanisms remain scarce. Physiological indices and histology of skeletal muscle were analyzed in SAMP8 mice at different ages. SAMP8 mice exhibited typical features of sarc...
journal_title:Biogerontology
pub_type: 杂志文章
doi:10.1007/s10522-020-09867-x
更新日期:2020-06-01 00:00:00
abstract::Human aging processes are regulated by many divergent pathways and on many levels. Thus, to understand such a complex system and define conserved mechanisms of aging, the use of cell culture-based models is a widespread practice. An often stated advantage of in vitro aging of primary cells is the high reproducibility ...
journal_title:Biogerontology
pub_type: 杂志文章
doi:10.1007/s10522-007-9082-x
更新日期:2007-08-01 00:00:00
abstract::Ageing leads to dramatic changes in the physiology of many different tissues resulting in a spectrum of pathology. Nonetheless, many lines of evidence suggest that ageing is driven by highly conserved cell intrinsic processes, and a set of unifying hallmarks of ageing has been defined. Here, we survey reports of age-l...
journal_title:Biogerontology
pub_type: 杂志文章,评审
doi:10.1007/s10522-018-9750-z
更新日期:2018-12-01 00:00:00
abstract::Aging is associated with accumulation of toxic intracellular and extracellular protein aggregates. Cells manage "aged" proteins by mobilizing their molecular chaperones or heat shock proteins that are also considered as determinants of lifespan in diverse species. In this study, we tested whether an exogenous addition...
journal_title:Biogerontology
pub_type: 杂志文章
doi:10.1007/s10522-008-9136-8
更新日期:2008-08-01 00:00:00
abstract::Deterioration of adipocyte function due to increased oxidative stress predisposes patients to metabolic disorders in advanced age. However, the roles of tumor suppressors in such conditions remain largely unknown. Therefore, we aimed to address their dynamics in aged adipocytes using a long-term culture model. We comp...
journal_title:Biogerontology
pub_type: 杂志文章
doi:10.1007/s10522-016-9661-9
更新日期:2017-02-01 00:00:00
abstract::For a period of 6-15 days prior to death, the fecundity and virility of Drosophila melanogaster fall significantly below those of same-aged flies that are not near death. It is likely that other aspects of physiology may decline during this period. This study attempts to document changes in two physiological character...
journal_title:Biogerontology
pub_type: 杂志文章
doi:10.1007/s10522-012-9398-z
更新日期:2012-10-01 00:00:00
abstract::Changes in the levels of O-linked N-acetyl-glucosamine (O-GlcNAc) on nucleocytoplasmic protein have been associated with a number of age-related diseases such as Alzheimer's and diabetes; however, there is relatively little information regarding the impact of age on tissue O-GlcNAc levels. Therefore, the goal of this ...
journal_title:Biogerontology
pub_type: 杂志文章
doi:10.1007/s10522-007-9123-5
更新日期:2008-06-01 00:00:00
abstract::Glycolysis and oxidative phosphorylation (OxPhos) are the two major mechanisms involved in brain energetics. In this article we propose that the sporadic forms of Alzheimer's disease (AD) are driven by age-related damage to macromolecules and organelles which results in the following series of dynamic processes. (1) M...
journal_title:Biogerontology
pub_type: 杂志文章
doi:10.1007/s10522-012-9403-6
更新日期:2012-12-01 00:00:00
abstract::In the last decades, studies about ageing have become more essential as our population grows older. The incidence of age-related diseases increases, which pose challenges both for societies and individuals in terms of life quality and economic impact. Understanding ageing and ageing-related processes will help us to s...
journal_title:Biogerontology
pub_type: 杂志文章,评审
doi:10.1007/s10522-018-9748-6
更新日期:2018-07-01 00:00:00
abstract::Death-associated protein 6 (DAXX) is a ubiquitous protein implicated in various cellular processes such as apoptosis, tumorigenesis, development and transcription. The role of DAXX is however ambiguous and many contradictory results regarding its function in apoptosis upon various cellular stresses are described in th...
journal_title:Biogerontology
pub_type: 杂志文章
doi:10.1007/s10522-016-9651-y
更新日期:2016-11-01 00:00:00
abstract::Two sibling Drosophila species dramatically divergent in longevity, Drosophila buzzatii and D. koepferae, were examined for possible effects of both developmental culture medium and dietary composition (DC) on longevity. Longevity was greatly increased in the longer lived D. buzzatii when flies were reared and fed on ...
journal_title:Biogerontology
pub_type: 杂志文章
doi:10.1007/s10522-013-9441-8
更新日期:2013-08-01 00:00:00
abstract::Dietary restriction (DR) exerts significant beneficial effects in terms of aging and age-related diseases in many organisms including humans. The present study aimed to examine the influence of long-term DR on the BDNF system at the transcriptional and translational levels in the cortex and hippocampus of middle-aged ...
journal_title:Biogerontology
pub_type: 杂志文章
doi:10.1007/s10522-014-9537-9
更新日期:2015-02-01 00:00:00
abstract::The long-term effects of X-irradiation with 0.25, 0.5, 0.75 and 1 Gy of 1 h eggs on the fitness-related life history traits in adult Drosophila melanogaster fruit flies and their offspring were investigated. Following irradiation with 0.25, 0.5 and 0.75 Gy, both F0 and F1 flies have decreased adult body weight and inc...
journal_title:Biogerontology
pub_type: 杂志文章
doi:10.1007/s10522-004-2571-2
更新日期:2004-01-01 00:00:00
abstract::Evolutionary theory leads to the general expectation that dietary restriction will often result in increased survival probabilities, and thus increased lifespan. The reaction norm is a basic tool of evolutionary analysis that quantifies the relationship between environmental parameters and functional characters, inclu...
journal_title:Biogerontology
pub_type: 杂志文章
doi:10.1007/s10522-006-9005-2
更新日期:2006-06-01 00:00:00
abstract::The human immune system, especially the adaptive branch, substantially declines with ageing. Several distinct immunosenescent events have already been described, yet data regarding to age-associated baseline alterations in immune cell function is limited. Therefore, by using the TaqMan Human Immune Arrays we conducted...
journal_title:Biogerontology
pub_type: 杂志文章
doi:10.1007/s10522-010-9274-7
更新日期:2010-12-01 00:00:00
abstract::Reactive oxygen species (ROS) are formed by all aerobic organisms, and are involved in the numerous physiological and pathophysiological processes. Opioid peptides belong to a class of bioactive compounds of great interest because of their opiate-like activity. We determined the influence of methionine-enkephalin (MEN...
journal_title:Biogerontology
pub_type: 杂志文章
doi:10.1007/s10522-005-6040-3
更新日期:2006-02-01 00:00:00
abstract::We studied vascular function in quiescent aortas from senescence-accelerated resistant (SAM-R1) and prone (SAM-P8) mice. Myographical studies of thoracic aorta segments from 6-7 month-old mice showed that the contractility of SAM-P8 aortas was markedly higher than that of SAM-R1 after KCl depolarization or phenylephri...
journal_title:Biogerontology
pub_type: 杂志文章
doi:10.1007/s10522-007-9108-4
更新日期:2007-12-01 00:00:00
abstract::Hundreds of genes and miRNAs have been identified as being involved in the determination of longevity, aging patterns and in the development of age-related diseases (ARDs). The interplay between these genes as well as the role of miRNAs in the context of protein-protein interaction networks has as yet been poorly addr...
journal_title:Biogerontology
pub_type: 杂志文章
doi:10.1007/s10522-010-9265-8
更新日期:2010-08-01 00:00:00
abstract::After reaching adulthood, orderly repair is probably one of the most important mechanisms throughout lifetime. Regular wound healing after an injury consists of a well-ordered sequence of overlapping phases of repair and essentially repeats the complex process of organ development. Organ failure in the ageing organism...
journal_title:Biogerontology
pub_type: 杂志文章,评审
doi:10.1007/s10522-013-9453-4
更新日期:2013-12-01 00:00:00
abstract::Previously, extremely low, nanomolar concentrations of the mitochondria-targeted plastoquinone derivative SkQ1 (10-(6'-plastoquinonyl) decyltriphenylphosphonium) were shown to prolong the lifespan of male and female Drosophila melanogaster by about 10 % (Anisimov et al., Biochemistry (Moscow) 73:1329-1342, 2008). Usin...
journal_title:Biogerontology
pub_type: 杂志文章
doi:10.1007/s10522-012-9404-5
更新日期:2012-12-01 00:00:00
abstract::In mammals suprachiasmatic nucleus (SCN), acts as a light entrainable master clock and by generation of temporal oscillations regulates the peripheral organs acting as autonomous clocks resulting in overt behavioral and physiological rhythms. SCN also controls synthesis and release of melatonin (hormonal message for d...
journal_title:Biogerontology
pub_type: 杂志文章
doi:10.1007/s10522-016-9656-6
更新日期:2016-11-01 00:00:00
abstract::Invariant natural killer T (iNKT) cells represent a well-established T cell lineage characterised in humans by TCR consisting of an invariant alpha chain encoded by Valpha24-JalphaQ genes, paired preferentially with a Vbeta11 chain. iNKT cells also share some characteristics with NK cells, such as the expression of th...
journal_title:Biogerontology
pub_type: 杂志文章
doi:10.1007/s10522-006-9063-5
更新日期:2006-10-01 00:00:00
abstract::Cell senescence, a state of cell cycle arrest and altered metabolism with enhanced pro-inflammatory secretion, underlies at least some aspects of organismal ageing. The sirtuin family of deacetylases has been implicated in preventing premature ageing; sirtuin overexpression or resveratrol-mediated activation of sirtui...
journal_title:Biogerontology
pub_type: 杂志文章
doi:10.1007/s10522-018-09792-0
更新日期:2019-06-01 00:00:00
abstract::Idebenone, the synthetic analog of coenzyme Q10 can improve electron transport in mitochondria. Therefore, it is used in the treatment of Alzheimer's disease and other cognitive impairments. However, the mechanism of its action on neurodevelopment is still to be elucidated. Here we demonstrate that the cellular respon...
journal_title:Biogerontology
pub_type: 杂志文章
doi:10.1007/s10522-017-9718-4
更新日期:2017-08-01 00:00:00
abstract::Extracellular matrix (ECM) organization is a complex process that requires the coordinated efforts of many molecules. For the regulation of collagen fiber diameter, the proteoglycan decorin appears to be of major relevance. To investigate the role of decorin in the process of (photo-)aging in more detail, full-thickne...
journal_title:Biogerontology
pub_type: 杂志文章
doi:10.1007/s10522-006-9070-6
更新日期:2007-06-01 00:00:00
abstract::Lung injuries are generally more serious and cause high mortality in aged humans and animals. Heme oxygenase-1 (HO-1) is known to be readily inducible in alveolar macrophages (AMs) and airway epithelial cells to confer cytoprotection against oxidative stress. We thus investigated whether aging impairs the stress-induc...
journal_title:Biogerontology
pub_type: 杂志文章
doi:10.1007/s10522-008-9164-4
更新日期:2009-04-01 00:00:00
abstract::Aging process or senescence affects the expression of a wide range of phenotypic traits throughout the life span of organisms. These traits often show modular, synergistic, and even antagonistic relationships, and are also influenced by genomic, developmental, physiological and environmental factors. The cardiovascula...
journal_title:Biogerontology
pub_type: 杂志文章
doi:10.1007/s10522-013-9486-8
更新日期:2014-04-01 00:00:00