Simultaneous co-localized super-resolution fluorescence microscopy and atomic force microscopy: combined SIM and AFM platform for the life sciences.

Abstract:

:Correlating data from different microscopy techniques holds the potential to discover new facets of signaling events in cellular biology. Here we report for the first time a hardware set-up capable of achieving simultaneous co-localized imaging of spatially correlated far-field super-resolution fluorescence microscopy and atomic force microscopy, a feat only obtained until now by fluorescence microscopy set-ups with spatial resolution restricted by the Abbe diffraction limit. We detail system integration and demonstrate system performance using sub-resolution fluorescent beads and applied to a test sample consisting of human bone osteosarcoma epithelial cells, with plasma membrane transporter 1 (MCT1) tagged with an enhanced green fluorescent protein (EGFP) at the N-terminal.

journal_name

Sci Rep

journal_title

Scientific reports

authors

Gómez-Varela AI,Stamov DR,Miranda A,Alves R,Barata-Antunes C,Dambournet D,Drubin DG,Paiva S,De Beule PAA

doi

10.1038/s41598-020-57885-z

subject

Has Abstract

pub_date

2020-01-24 00:00:00

pages

1122

issue

1

issn

2045-2322

pii

10.1038/s41598-020-57885-z

journal_volume

10

pub_type

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