Abstract:
:Correlating data from different microscopy techniques holds the potential to discover new facets of signaling events in cellular biology. Here we report for the first time a hardware set-up capable of achieving simultaneous co-localized imaging of spatially correlated far-field super-resolution fluorescence microscopy and atomic force microscopy, a feat only obtained until now by fluorescence microscopy set-ups with spatial resolution restricted by the Abbe diffraction limit. We detail system integration and demonstrate system performance using sub-resolution fluorescent beads and applied to a test sample consisting of human bone osteosarcoma epithelial cells, with plasma membrane transporter 1 (MCT1) tagged with an enhanced green fluorescent protein (EGFP) at the N-terminal.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Gómez-Varela AI,Stamov DR,Miranda A,Alves R,Barata-Antunes C,Dambournet D,Drubin DG,Paiva S,De Beule PAAdoi
10.1038/s41598-020-57885-zsubject
Has Abstractpub_date
2020-01-24 00:00:00pages
1122issue
1issn
2045-2322pii
10.1038/s41598-020-57885-zjournal_volume
10pub_type
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