当前位置： SCI文献检索 > INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES期刊下所有文献 > Platelet Adhesion and Thrombus Formation in Microchannels: The Effect of Assay-Dependent Variables.

Platelet Adhesion and Thrombus Formation in Microchannels: The Effect of Assay-Dependent Variables.

Abstract：

:Microfluidic flow chambers (MFCs) allow the study of platelet adhesion and thrombus formation under flow, which may be influenced by several variables. We developed a new MFC, with which we tested the effects of different variables on the results of platelet deposition and thrombus formation on a collagen-coated surface. METHODS:Whole blood was perfused in the MFC over collagen Type I for 4 min at different wall shear rates (WSR) and different concentrations of collagen-coating solutions, keeping blood samples at room temperature or 37 °C before starting the experiments. In addition, we tested the effects of the antiplatelet agent acetylsalicylic acid (ASA) (antagonist of cyclooxygenase-1, 100 µM) and cangrelor (antagonist of P2Y12, 1 µM). RESULTS:Platelet deposition on collagen (I) was not affected by the storage temperature of the blood before perfusion (room temperature vs. 37 °C); (II) was dependent on a shear rate in the range between 300/s and 1700/s; and (III) was influenced by the collagen concentration used to coat the microchannels up to a value of 10 µg/mL. ASA and cangrelor did not cause statistically significant inhibition of platelet accumulation, except for ASA at low collagen concentrations. CONCLUSIONS:Platelet deposition on collagen-coated surfaces is a shear-dependent process, not influenced by the collagen concentration beyond a value of 10 µg/mL. However, the inhibitory effect of antiplatelet drugs is better observed using low concentrations of collagen.

journal_name

Int J Mol Sci

journal_title

International journal of molecular sciences

authors

Scavone M,Bozzi S,Mencarini T,Podda G,Cattaneo M,Redaelli A

doi

10.3390/ijms21030750

subject

Has Abstract

pub_date

2020-01-23 00:00:00

issue

3

issn

1422-0067

pii

ijms21030750

journal_volume

21

pub_type

杂志文章

相关文献

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES文献大全
  • Schiff Base Ancillary Ligands in Bis(diimine) Copper(I) Dye-Sensitized Solar Cells.

    abstract：:Five 6,6'-dimethyl-2,2'-bipyridine ligands bearing N-arylmethaniminyl substituents in the 4- and 4'-positions were prepared by Schiff base condensation in which the aryl group is Ph (1), 4-tolyl (2), 4- t BuC6H4 (3), 4-MeOC6H4 (4), and 4-Me2NC6H4 (5). The homoleptic copper(I...

    journal_title：International journal of molecular sciences

    pub_type： 杂志文章

    doi：10.3390/ijms21051735

    authors： Lüthi E,Forero Cortés PA,Prescimone A,Constable EC,Housecroft CE

    更新日期：2020-03-03 00:00:00

  • Overexpression of S-Adenosyl-l-Methionine Synthetase 2 from Sugar Beet M14 Increased Arabidopsis Tolerance to Salt and Oxidative Stress.

    abstract：:The sugar beet monosomic addition line M14 is a unique germplasm that contains genetic materials from Beta vulgaris L. and Beta corolliflora Zoss, and shows tolerance to salt stress. Our study focuses on exploring the molecular mechanism of the salt tolerance of the sugar beet M14. In order to identify differentially ...

    journal_title：International journal of molecular sciences

    pub_type： 杂志文章

    doi：10.3390/ijms18040847

    authors： Ma C,Wang Y,Gu D,Nan J,Chen S,Li H

    更新日期：2017-04-18 00:00:00

  • Human Chorionic Gonadotrophin as a Possible Mediator of Leiomyoma Growth during Pregnancy: Molecular Mechanisms.

    abstract：:Uterine fibroids are the most common gynecologic benign tumors. Studies supporting a strong pregnancy-related growth of leiomyomas generally claimed a crucial role of sex steroid hormones. However, sex steroids are unlikely the unique actors involved as estrogen and progesterone achieve a pick serum concentration in t...

    journal_title：International journal of molecular sciences

    pub_type： 杂志文章,评审

    doi：10.3390/ijms18092014

    authors： Sarais V,Cermisoni GC,Schimberni M,Alteri A,Papaleo E,Somigliana E,Vigano' P

    更新日期：2017-09-20 00:00:00

  • Insect Cecropins, Antimicrobial Peptides with Potential Therapeutic Applications.

    abstract：:The alarming escalation of infectious diseases resistant to conventional antibiotics requires urgent global actions, including the development of new therapeutics. Antimicrobial peptides (AMPs) represent potential alternatives in the treatment of multi-drug resistant (MDR) infections. Here, we focus on Cecropins (Cecs...

    journal_title：International journal of molecular sciences

    pub_type： 杂志文章,评审

    doi：10.3390/ijms20235862

    authors： Brady D,Grapputo A,Romoli O,Sandrelli F

    更新日期：2019-11-22 00:00:00

  • Autocrine TGF-β in Cancer: Review of the Literature and Caveats in Experimental Analysis.

    abstract：:Autocrine signaling is defined as the production and secretion of an extracellular mediator by a cell followed by the binding of that mediator to receptors on the same cell to initiate signaling. Autocrine stimulation often operates in autocrine loops, a type of interaction, in which a cell produces a mediator, for wh...

    journal_title：International journal of molecular sciences

    pub_type： 杂志文章,评审

    doi：10.3390/ijms22020977

    authors： Ungefroren H

    更新日期：2021-01-19 00:00:00

  • RAGE Signaling in Melanoma Tumors.

    abstract：:Despite recent progresses in its treatment, malignant cutaneous melanoma remains a cancer with very poor prognosis. Emerging evidences suggest that the receptor for advance glycation end products (RAGE) plays a key role in melanoma progression through its activation in both cancer and stromal cells. In tumors, RAGE ac...

    journal_title：International journal of molecular sciences

    pub_type： 杂志文章,评审

    doi：10.3390/ijms21238989

    authors： Olaoba OT,Kadasah S,Vetter SW,Leclerc E

    更新日期：2020-11-26 00:00:00

  • Resistin stimulates expression of chemokine genes in chondrocytes via combinatorial regulation of C/EBPβ and NF-κB.

    abstract：:To further investigate the regulation role of two chemokine genes CCL3 and CCL4 in chondrocytes in response to resistin, human primary chondrocytes and T/C-28a2 cells were cultured. The function of resistin on the chemokine genes, and the expression of C/EBPβ, NF-κB isoforms were tested using qPCR. The methods used to...

    journal_title：International journal of molecular sciences

    pub_type： 杂志文章

    doi：10.3390/ijms151017242

    authors： Zhang Z,Zhang Z,Kang Y,Hou C,Duan X,Sheng P,Sandell LJ,Liao W

    更新日期：2014-09-26 00:00:00

  • Some Biological Consequences of the Inhibition of Na,K-ATPase by Translationally Controlled Tumor Protein (TCTP).

    abstract：:Na,K-ATPase is an ionic pump that regulates the osmotic equilibrium and membrane potential of cells and also functions as a signal transducer. The interaction of Na,K-ATPase with translationally controlled tumor protein (TCTP) results, among others, in the inhibition of the former's pump activity and in the initiation...

    journal_title：International journal of molecular sciences

    pub_type： 杂志文章,评审

    doi：10.3390/ijms19061657

    authors： Jung J,Ryu S,Ki IA,Woo HA,Lee K

    更新日期：2018-06-04 00:00:00

  • Genetic differentiation between natural and hatchery stocks of Japanese scallop (Mizuhopecten yessoensis) as revealed by AFLP analysis.

    abstract：:Japanese scallop (Mizuhopecten yessoensis) is a cold-tolerant bivalve that was introduced to China for aquaculture in 1982. In this study, amplified fragment length polymorphism (AFLP) markers were used to investigate levels of genetic diversity within M. yessoensis cultured stocks and compare them with wild populatio...

    journal_title：International journal of molecular sciences

    pub_type： 杂志文章

    doi：10.3390/ijms11103933

    authors： Liu WD,Li HJ,Bao XB,Gao XG,Li YF,He CB,Liu ZJ

    更新日期：2010-10-15 00:00:00

  • Renaissance Distribution for Statistically Failed Experiments.

    abstract：:Much of the experimental data, especially in life sciences, is considered to be useless if it demonstrates a large standard deviation from the mean value. The Renaissance distribution, as presented in this study, allows one to extract true values from such statistical data with large noise. To obtain proof of the Rena...

    journal_title：International journal of molecular sciences

    pub_type： 杂志文章

    doi：10.3390/ijms20133250

    authors： Popov R,Shankara GK,von Bojničić-Kninski C,Nesterov-Mueller A

    更新日期：2019-07-02 00:00:00

  • Radiolabeled Human Monoclonal Antibody 067-213 has the Potential for Noninvasive Quantification of CD73 Expression.

    abstract：BACKGROUND:CD73 is an ectonucleotidase regulating extracellular adenosine concentration and plays an important role in adenosine-mediated immunosuppressive pathways. The efficacy of CD73-targeted therapy depends on the expression levels of CD73; therefore, monitoring CD73 status in cancer patients would provide helpful...

    journal_title：International journal of molecular sciences

    pub_type： 杂志文章

    doi：10.3390/ijms21072304

    authors： Sudo H,Tsuji AB,Sugyo A,Kurosawa G,Kurosawa Y,Alexander D,Tsuda H,Saga T,Higashi T

    更新日期：2020-03-26 00:00:00

  • Molecular Insight into Gut Microbiota and Rheumatoid Arthritis.

    abstract：:Rheumatoid arthritis (RA) is a systemic, inflammatory, and autoimmune disorder. Gut microbiota play an important role in the etiology of RA. With the considerable progress made in next-generation sequencing techniques, the identified gut microbiota difference between RA patients and healthy individuals provides an upd...

    journal_title：International journal of molecular sciences

    pub_type： 杂志文章,评审

    doi：10.3390/ijms17030431

    authors： Wu X,He B,Liu J,Feng H,Ma Y,Li D,Guo B,Liang C,Dang L,Wang L,Tian J,Zhu H,Xiao L,Lu C,Lu A,Zhang G

    更新日期：2016-03-22 00:00:00

  • Analgesic Mechanisms of Antidepressants for Neuropathic Pain.

    abstract：:Tricyclic antidepressants and serotonin noradrenaline reuptake inhibitors are used to treat chronic pain, such as neuropathic pain. Why antidepressants are effective for treatment of neuropathic pain and the precise mechanisms underlying their effects, however, remain unclear. The inhibitory effects of these antidepre...

    journal_title：International journal of molecular sciences

    pub_type： 杂志文章,评审

    doi：10.3390/ijms18112483

    authors： Obata H

    更新日期：2017-11-21 00:00:00

  • Age dependent changes in cartilage matrix, subchondral bone mass, and estradiol levels in blood serum, in naturally occurring osteoarthritis in Guinea pigs.

    abstract：:The Dunkin Hartley (DH) guinea pig is a widely used naturally occurring osteoarthritis model. The aim of this study was to provide detailed evidence of age-related changes in articular cartilage, subchondral bone mineral density, and estradiol levels. We studied the female Dunkin Hartley guinea pigs at 1, 3, 6, 9, and...

    journal_title：International journal of molecular sciences

    pub_type： 杂志文章

    doi：10.3390/ijms150813578

    authors： Yan JY,Tian FM,Wang WY,Cheng Y,Xu HF,Song HP,Zhang YZ,Zhang L

    更新日期：2014-08-05 00:00:00

  • Roles of Sestrin2 and Ribosomal Protein S6 in Transient Global Ischemia-Induced Hippocampal Neuronal Injury.

    abstract：:Recent studies suggested that sestrin2 is a crucial modulator for the production of reactive oxygen species (ROS). In addition, sestrin2 may also regulate ribosomal protein S6 (RpS6), a molecule important for protein synthesis, through the effect of mammalian target of rapamycin (mTOR) complex that is pivotal for long...

    journal_title：International journal of molecular sciences

    pub_type： 杂志文章

    doi：10.3390/ijms161125963

    authors： Chuang YC,Yang JL,Yang DI,Lin TK,Liou CW,Chen SD

    更新日期：2015-11-04 00:00:00

  • IFN-β: A Contentious Player in Host-Pathogen Interaction in Tuberculosis.

    abstract：:Tuberculosis (TB) is a major health threat to the human population worldwide. The etiology of the disease is Mycobacterium tuberculosis (Mtb), a highly successful intracellular pathogen. It has the ability to manipulate the host immune response and to make the intracellular environment suitable for its survival. Many ...

    journal_title：International journal of molecular sciences

    pub_type： 杂志文章,评审

    doi：10.3390/ijms18122725

    authors： Sabir N,Hussain T,Shah SZA,Zhao D,Zhou X

    更新日期：2017-12-16 00:00:00

  • Characterization and comparison of Fumonisin B(1)-protein conjugates by six methods.

    abstract：:In order to generate an antibody against a small hapten molecule, the hapten is cross-linked with carrier protein to make it immunogenic. In this study, the hapten (Fumonisin B(1), FB(1)) was coupled to ovalbumin (OVA) and bovine serum albumin (BSA), respectively by a short cross-linker reagent (glutaraldehyde, GA). T...

    journal_title：International journal of molecular sciences

    pub_type： 杂志文章

    doi：10.3390/ijms13010084

    authors： Wang Y,He CH,Zheng H,Zhang HB

    更新日期：2012-01-01 00:00:00

  • Enriched Conformational Sampling of DNA and Proteins with a Hybrid Hamiltonian Derived from the Protein Data Bank.

    abstract：:In this article, we present a method for the enhanced molecular dynamics simulation of protein and DNA systems called potential of mean force (PMF)-enriched sampling. The method uses partitions derived from the potentials of mean force, which we determined from DNA and protein structures in the Protein Data Bank (PDB)...

    journal_title：International journal of molecular sciences

    pub_type： 杂志文章

    doi：10.3390/ijms19113405

    authors： Peter EK,Cerny J

    更新日期：2018-10-30 00:00:00

  • The Essential Role of Pin1 via NF-κB Signaling in Vascular Inflammation and Atherosclerosis in ApoE-/- Mice.

    abstract：:Atherosclerosis, as a chronic inflammatory disease, is the major underlying cause of death worldwide. However, the mechanisms that underlie the inflammatory process are not completely understood. Prolyl-isomerase-1 (Pin1), as a unique peptidyl-prolyl isomerase, plays an important role in inflammation and endothelial d...

    journal_title：International journal of molecular sciences

    pub_type： 杂志文章

    doi：10.3390/ijms18030644

    authors： Liu M,Yu P,Jiang H,Yang X,Zhao J,Zou Y,Ge J

    更新日期：2017-03-16 00:00:00

  • Chronic Sildenafil Treatment Improves Vasomotor Function in a Mouse Model of Accelerated Aging.

    abstract：:Aging leads to a loss of vasomotor control. Both vasodilation and vasoconstriction are affected. Decreased nitric oxide-cGMP-mediated relaxation is a hallmark of aging. It contributes to vascular disease, notably hypertension, infarction, and dementia. Decreased vasodilation can be caused by aging independently from c...

    journal_title：International journal of molecular sciences

    pub_type： 杂志文章

    doi：10.3390/ijms21134667

    authors： Golshiri K,Ataei Ataabadi E,Brandt R,van der Pluijm I,de Vries R,Danser J,Roks A

    更新日期：2020-06-30 00:00:00

  • Characterization of the interaction between eupatorin and bovine serum albumin by spectroscopic and molecular modeling methods.

    abstract：:This study investigated the interaction between eupatorin and bovine serum albumin (BSA) using ultraviolet-visible (UV-vis) absorption, fluorescence, synchronous fluorescence, circular dichroism (CD) spectroscopies, and molecular modeling at pH 7.4. Results of UV-vis and fluorescence spectroscopies illustrated that BS...

    journal_title：International journal of molecular sciences

    pub_type： 杂志文章

    doi：10.3390/ijms140714185

    authors： Xu H,Yao N,Xu H,Wang T,Li G,Li Z

    更新日期：2013-07-09 00:00:00

  • The Epigenetic Landscape of Vascular Calcification: An Integrative Perspective.

    abstract：:Vascular calcification (VC) is an important complication among patients of advanced age, those with chronic kidney disease, and those with diabetes mellitus. The pathophysiology of VC encompasses passive occurrence of physico-chemical calcium deposition, active cellular secretion of osteoid matrix upon exposure to met...

    journal_title：International journal of molecular sciences

    pub_type： 杂志文章,评审

    doi：10.3390/ijms21030980

    authors： Hou YC,Lu CL,Yuan TH,Liao MT,Chao CT,Lu KC

    更新日期：2020-02-01 00:00:00

  • Growth Hormone Deficiency Following Traumatic Brain Injury.

    abstract：:Traumatic brain injury (TBI) is fairly common and annually affects millions of people worldwide. Post traumatic hypopituitarism (PTHP) has been increasingly recognized as an important and prevalent clinical entity. Growth hormone deficiency (GHD) is the most common pituitary hormone deficit in long-term survivors of T...

    journal_title：International journal of molecular sciences

    pub_type： 杂志文章,评审

    doi：10.3390/ijms20133323

    authors： Kgosidialwa O,Hakami O,Muhammad Zia-Ul-Hussnain H,Agha A

    更新日期：2019-07-06 00:00:00

  • Adiponectin in Chronic Kidney Disease.

    abstract：:Adiponectin is the adipokine associated with insulin sensitization, reducing liver gluconeogenesis, and increasing fatty acid oxidation and glucose uptake. Adiponectin is present in the kidneys, mainly in the arterial endothelium and smooth muscle cells, as well as in the capillary endothelium, and might be considered...

    journal_title：International journal of molecular sciences

    pub_type： 杂志文章,评审

    doi：10.3390/ijms21249375

    authors： Przybyciński J,Dziedziejko V,Puchałowicz K,Domański L,Pawlik A

    更新日期：2020-12-09 00:00:00

  • α-Tocopherol at nanomolar concentration protects PC12 cells from hydrogen peroxide-induced death and modulates protein kinase activities.

    abstract：:The aim of this work was to compare protective and anti-apoptotic effects of α-tocopherol at nanomolar and micromolar concentrations against 0.2 mM H(2)O(2)-induced toxicity in the PC12 neuronal cell line and to reveal protein kinases that contribute to α-tocopherol protective action. The protection by 100 nM α-tocoph...

    journal_title：International journal of molecular sciences

    pub_type： 杂志文章

    doi：10.3390/ijms130911543

    authors： Zakharova IO,Sokolova TV,Bayunova LV,Vlasova YA,Rychkova MP,Avrova NF

    更新日期：2012-01-01 00:00:00

  • cGMP Signaling in the Cardiovascular System-The Role of Compartmentation and Its Live Cell Imaging.

    abstract：:The ubiquitous second messenger 3',5'-cyclic guanosine monophosphate (cGMP) regulates multiple physiologic processes in the cardiovascular system. Its intracellular effects are mediated by stringently controlled subcellular microdomains. In this review, we will illustrate the current techniques available for real-time...

    journal_title：International journal of molecular sciences

    pub_type： 杂志文章,评审

    doi：10.3390/ijms19030801

    authors： Bork NI,Nikolaev VO

    更新日期：2018-03-10 00:00:00

  • The Opposite Effect of c-Jun Transcription Factor on Apolipoprotein E Gene Regulation in Hepatocytes and Macrophages.

    abstract：:Apolipoprotein E (apoE) is mainly secreted by hepatocytes and incorporated into most plasma lipoproteins. Macrophages, which accumulate cholesterol and are critical for the development of the atherosclerotic plaque, are also an important, albeit smaller, apoE source. Distal regulatory elements control cell-specific ac...

    journal_title：International journal of molecular sciences

    pub_type： 杂志文章

    doi：10.3390/ijms20061471

    authors： Trusca VG,Fuior EV,Kardassis D,Simionescu M,Gafencu AV

    更新日期：2019-03-23 00:00:00

  • Application of the SwissDrugDesign Online Resources in Virtual Screening.

    abstract：:SwissDrugDesign is an important initiative led by the Molecular Modeling Group of the SIB Swiss Institute of Bioinformatics. This project provides a collection of freely available online tools for computer-aided drug design. Some of these web-based methods, i.e., SwissSimilarity and SwissTargetPrediction, were especia...

    journal_title：International journal of molecular sciences

    pub_type： 杂志文章,评审

    doi：10.3390/ijms20184612

    authors： Daina A,Zoete V

    更新日期：2019-09-18 00:00:00

  • Dietary Supplementation with Lactobacillus casei Alleviates Lipopolysaccharide-Induced Liver Injury in a Porcine Model.

    abstract：:This study aims to determine whether Lactobacillus casei (L. casei) could relieve liver injury in piglets challenged with lipopolysaccharide (LPS). Piglets were randomly allocated into one of the three groups: control, LPS, and L. casei. The control and LPS groups were fed a corn- and soybean meal-based diet, whereas ...

    journal_title：International journal of molecular sciences

    pub_type： 杂志文章

    doi：10.3390/ijms18122535

    authors： Zhao D,Wu T,Yi D,Wang L,Li P,Zhang J,Hou Y,Wu G

    更新日期：2017-11-26 00:00:00

  • Histamine H2-Receptor Antagonists Improve Non-Steroidal Anti-Inflammatory Drug-Induced Intestinal Dysbiosis.

    abstract：:Dysbiosis, an imbalance of intestinal flora, can cause serious conditions such as obesity, cancer, and psychoneurological disorders. One cause of dysbiosis is inflammation. Ulcerative enteritis is a side effect of non-steroidal anti-inflammatory drugs (NSAIDs). To counteract this side effect, we proposed the concurren...

    journal_title：International journal of molecular sciences

    pub_type： 杂志文章

    doi：10.3390/ijms21218166

    authors： Kawashima R,Tamaki S,Kawakami F,Maekawa T,Ichikawa T

    更新日期：2020-10-31 00:00:00