Single-cell RNA sequencing of Trypanosoma brucei from tsetse salivary glands unveils metacyclogenesis and identifies potential transmission blocking antigens.

abstract：:The saturation values for ribosomal RNA hybridization have been determined for DNA from diploid and polytene tissues of Drosophila melanogaster. These values have been measured in XO and XX larvae which have, respectively, one and two nucleolus organizers in the diploid chromosome set. The results show that (1) in dip...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Spear BB,Gall JG

更新日期：1973-05-01 00:00:00

abstract：:We give a functional generalization of fractal scaling laws applied to response problems as well as to probability distributions. We consider excitations and responses, which are functions of a given state vector. Based on scaling arguments, we derive a general nonlinear response functional scaling law, which expresse...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Vlad MO,Morán F,Popa VT,Szedlacsek SE,Ross J

更新日期：2007-03-20 00:00:00

abstract：:We have determined the localization and orientation of two genetic probes within the human major histocompatibility complex by chromosomal in situ hybridization. Our data indicate that a cloned genomic probe cross-hybridizing to HLA-A, -B, and -C heavy chain loci is homologous to sequences located on chromosome 6 at b...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Morton CC,Kirsch IR,Nance WE,Evans GA,Korman AJ,Strominger JL

更新日期：1984-05-01 00:00:00

abstract：:We have examined whether dissociation of the histone octamer is required for elongation of RNA transcripts through arrays of nucleosome cores in vitro. Control or dimethyl suberimidate-crosslinked histone octamers were reconstituted onto supercoiled, closed circular pT207-18 DNA, which contains tandemly repeated 207-b...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： O'Neill TE,Smith JG,Bradbury EM

更新日期：1993-07-01 00:00:00

abstract：:Electrogenic sodium- and chloride-dependent gamma-aminobutyric acid (GABA) transport in crude synaptosomal membrane vesicles is partly inhibited by saturating levels of either of the substrate analogues cis-3-aminocyclohexanecarboxylic acid (ACHC) or beta-alanine. However, both of them together potently and fully inhi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kanner BI,Bendahan A

更新日期：1990-04-01 00:00:00

abstract：:To elucidate the roles of IgE antibody in host responses to helminth infection, selective IgE-deficient SJA/9 mice were infected with the nematode parasites Nippostrongylus brasiliensis, which elicits remarkable potentiated IgE production, and Trichinella spiralis, which induces strong anti-Trichinella IgE antibody pr...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Watanabe N,Katakura K,Kobayashi A,Okumura K,Ovary Z

更新日期：1988-06-01 00:00:00

abstract：:Previous findings of intact remote autobiographical memory in patients with medial temporal lobe damage have been questioned on the grounds that the narrative recollections were impoverished and fact-like and that the methods were not sufficiently sensitive to detect an impairment. We adopted a newer method, the Autob...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kirwan CB,Bayley PJ,Galván VV,Squire LR

更新日期：2008-02-19 00:00:00

abstract：:Chronic morphine administration is associated with the development of tolerance, both clinically and in animal models. Many assume that tolerance is a continually progressive response to chronic opioid dosing. However, clinicians have long appreciated the ability to manage cancer pain in patients for months on stable ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Xu J,Faskowitz AJ,Rossi GC,Xu M,Lu Z,Pan YX,Pasternak GW

更新日期：2015-01-06 00:00:00

abstract：:A biological membrane is conceptualized as a system in which membrane proteins are naturally matched to the equilibrium thickness of the lipid bilayer. Cholesterol, in addition to lipid composition, has been suggested to be a major regulator of bilayer thickness in vivo because measurements in vitro have shown that ch...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mitra K,Ubarretxena-Belandia I,Taguchi T,Warren G,Engelman DM

更新日期：2004-03-23 00:00:00

abstract：:Functional lysosomes mediate autophagy and macropinocytosis for nutrient acquisition. Pancreatic ductal adenocarcinoma (PDAC) tumors exhibit high basal lysosomal activity, and inhibition of lysosome function suppresses PDAC cell proliferation and tumor growth. However, the codependencies induced by lysosomal inhibitio...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Elliott IA,Dann AM,Xu S,Kim SS,Abt ER,Kim W,Poddar S,Moore A,Zhou L,Williams JL,Capri JR,Ghukasyan R,Matsumura C,Tucker DA,Armstrong WR,Cabebe AE,Wu N,Li L,Le TM,Radu CG,Donahue TR

更新日期：2019-04-02 00:00:00

abstract：:Netrin-1 is critical for axonal pathfinding which shares similarities with formation of vascular network. Here we report that netrin-1 induction of angiogenesis is mediated by an increase in endothelial nitric oxide (NO*) production, which occurs via a DCC-dependent, ERK1/2-eNOS feed-forward mechanism. Exposure of mat...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Nguyen A,Cai H

更新日期：2006-04-25 00:00:00

abstract：:Mutations in WNK1 and WNK4 lead to familial hyperkalemic hypertension (FHHt). Because FHHt associates net positive Na(+) balance together with K(+) and H(+) renal retention, the identification of WNK1 and WNK4 led to a new paradigm to explain how aldosterone can promote either Na(+) reabsorption or K(+) secretion in a...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hadchouel J,Soukaseum C,Büsst C,Zhou XO,Baudrie V,Zürrer T,Cambillau M,Elghozi JL,Lifton RP,Loffing J,Jeunemaitre X

更新日期：2010-10-19 00:00:00

abstract：:Low phosphate (Pi) availability is one of the major constraints for plant productivity in natural and agricultural ecosystems. Plants have evolved a myriad of developmental and biochemical mechanisms to increase internal Pi uptake and utilization efficiency. One important biochemical pathway leading to an increase in ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Cruz-Ramírez A,Oropeza-Aburto A,Razo-Hernández F,Ramírez-Chávez E,Herrera-Estrella L

更新日期：2006-04-25 00:00:00

abstract：:Probing a wide range of cellular phenotypes in neurodevelopmental disorders using patient-derived neural progenitor cells (NPCs) can be facilitated by 3D assays, as 2D systems cannot entirely recapitulate the arrangement of cells in the brain. Here, we developed a previously unidentified 3D migration and differentiati...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zhang ZN,Freitas BC,Qian H,Lux J,Acab A,Trujillo CA,Herai RH,Nguyen Huu VA,Wen JH,Joshi-Barr S,Karpiak JV,Engler AJ,Fu XD,Muotri AR,Almutairi A

更新日期：2016-03-22 00:00:00

abstract：:Growth of 3T3-L2 fibroblasts in medium containing 5-bromo-2'-deoxyuridine prevents the spontaneous cytodifferentiation of these cells into adipose cells. Treatment of bromodeoxyuridine-suppressed cultures at confluence with 1-methyl-3-isobutylxanthine, a promoter of the adipose conversion, results in differentiation o...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Russell TR

更新日期：1979-09-01 00:00:00

abstract：:We have examined the use of our bis(sulfonamide) diol ligand (1) in the asymmetric addition of phenyl groups to cyclic alpha,beta-unsaturated ketones. Good to excellent enantioselectivities have been obtained with cyclic enones bearing alkyl substituents in the 2 position (71-97% enantiomeric excess). Furthermore, exc...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Li H,García C,Walsh PJ

更新日期：2004-04-13 00:00:00

abstract：:An extracellular matrix protein, cytotactin, with widespread tissue distribution has been identified, isolated, and partially characterized. Cytotactin mediates glia-neuron adhesion in vitro, but unlike Ng-CAM, the neuron-glia cell-adhesion molecule, it is absent from neurons. Cytotactin was isolated from 14-day embry...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Grumet M,Hoffman S,Crossin KL,Edelman GM

更新日期：1985-12-01 00:00:00

abstract：:Polyaromatic hydrocarbons are ubiquitous environmental chemicals that are important mutagens and carcinogens. The purpose of this study was to determine whether genes within the major histocompatibility complex (MHC) influence their biological activities. Cell-mediated immunity to dimethylbenz(a)anthracene (DMBA) was ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Elmets CA,Athar M,Tubesing KA,Rothaupt D,Xu H,Mukhtar H

更新日期：1998-12-08 00:00:00

abstract：:It has been widely reported that intrinsic brain activity, in a variety of animals including humans, is spatiotemporally structured. Specifically, propagated slow activity has been repeatedly demonstrated in animals. In human resting-state fMRI, spontaneous activity has been understood predominantly in terms of zero-l...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mitra A,Snyder AZ,Blazey T,Raichle ME

更新日期：2015-04-28 00:00:00

abstract：:Climate change and resource exploitation have been shown to modify the importance of bottom-up and top-down forces in ecosystems. However, the resulting pattern of trophic control in complex food webs is an emergent property of the system and thus unintuitive. We develop a statistical nondeterministic model, capable o...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lynam CP,Llope M,Möllmann C,Helaouët P,Bayliss-Brown GA,Stenseth NC

更新日期：2017-02-21 00:00:00

abstract：:The product of the Nijmegen breakage syndrome gene (NBS1) plays crucial roles in DNA damage response through its association with many proteins, including MRE11 and RAD50. However, it remains to be determined exactly how NBS1 accumulates at or near DNA double-strand breaks. Here we report that MDC1 directly binds to N...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wu L,Luo K,Lou Z,Chen J

更新日期：2008-08-12 00:00:00

abstract：:Murine endothelial cells are readily transformed in a single step by the polyomavirus oncogene encoding middle-sized tumor antigen. These cells (bEND.3) form tumors (hemangiomas) in mice which are lethal in newborn animals. The bEND.3 cells rapidly proliferate in culture and express little or no thrombospondin 1 (TS1)...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sheibani N,Frazier WA

更新日期：1995-07-18 00:00:00

abstract：:The 72-kDa spleen tyrosine kinase (Syk) and Src-family kinase p53/56Lyn (Lyn) contribute to signaling via the B-cell antigen receptor complex. Here we show that Syk and Lyn from human B lymphocytes can interact directly. Syk and Lyn coimmunoprecipitated from mature and activated B-cell lines, and gel-purified Syk and ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sidorenko SP,Law CL,Chandran KA,Clark EA

更新日期：1995-01-17 00:00:00

abstract：:The enzymic activity of crystalline mitochondrial aspartate aminotransferase (L-aspartate:2-oxoglutarate aminotransferase, EC 2.6.1.1) was determined in suspensions of noncrosslinked microcrystals in 30% (wt/vol) polyethylene glycol. The crystals (average dimensions, 22 x 5 x 0.8 micron) were small enough to preclude ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kirsten H,Gehring H,Christen P

更新日期：1983-04-01 00:00:00

abstract：:Rhythmic, spontaneously pulsating cardiac cells cultured from newborn rats are immediately stimulated to beat faster by addition of a number of tubulin-binding agents but not by their non-tubulin-binding analogues. The tubulin-binding agents tested include vinblastine, vincristine, navelbine, two analogs of vinblastin...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lampidis TJ,Kolonias D,Savaraj N,Rubin RW

更新日期：1992-02-15 00:00:00

abstract：:Cytochrome c oxidase, the terminal protein in the respiratory chain, converts oxygen into water and helps generate the electrochemical gradient used in the synthesis of ATP. The catalytic action of cytochrome c oxidase involves electron transfer, proton transfer, and O2 reduction. These events trigger specific molecul...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Nyquist RM,Heitbrink D,Bolwien C,Gennis RB,Heberle J

更新日期：2003-07-22 00:00:00

abstract：:A hierarchy of residue density assessments and packing properties in protein structures are contrasted, including a regular density, a variety of charge densities, a hydrophobic density, a polar density, and an aromatic density. These densities are investigated by alternative distance measures and also at the interfac...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Baud F,Karlin S

更新日期：1999-10-26 00:00:00

abstract：:For almost a century the plant hormone auxin has been central to theories on apical dominance, whereby the growing shoot tip suppresses the growth of the axillary buds below. According to the classic model, the auxin indole-3-acetic acid is produced in the shoot tip and transported down the stem, where it inhibits bud...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mason MG,Ross JJ,Babst BA,Wienclaw BN,Beveridge CA

更新日期：2014-04-22 00:00:00

abstract：:Bufonid anurans of the genus Atelopus contain both steroidal bufadienolides and various guanidinium alkaloids of the tetrodotoxin class. The former inhibit sodium-potassium ATPases, whereas the latter block voltage-dependent sodium channels. The structure of one guanidinium alkaloid, zetekitoxin AB, has remained a mys...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yotsu-Yamashita M,Kim YH,Dudley SC Jr,Choudhary G,Pfahnl A,Oshima Y,Daly JW

更新日期：2004-03-30 00:00:00

abstract：:NK cells are promising effectors for tumor adoptive immunotherapy, particularly when considering the targeting of MHC class I low or negative tumors. Yet, NK cells cannot respond to many tumors, which is particularly the case for nonhematopoietic tumors such as carcinomas or melanoma even when these cells lose MHC cla...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kruschinski A,Moosmann A,Poschke I,Norell H,Chmielewski M,Seliger B,Kiessling R,Blankenstein T,Abken H,Charo J

更新日期：2008-11-11 00:00:00