Abstract：
:In this study, we investigated if subunit displacement and/or alterations in proteasome biosynthesis are responsible for the changes in the levels of constitutive proteasomes (c-20S), immunoproteasomes (i-20S) and the activators PA28 and PA700 in neurons and astrocytes cultured with a cytokine mixture (IFN-γ/TNF-α/IL-1β). Exposure of both cell types to cytokines for 24 h increases mRNA and protein expression of the i-20S-specific subunit β5i and PA28α/β, and leads to a decline in the amount of the c-20S-specific subunit β5. Since β5 mRNA levels are unchanged by the cytokine treatment, it is fair to conclude that displacement of constitutive β-subunits with inducible β5i subunits is likely the mechanism underlying the decrease in c-20S. As expected, the increase in the amount of the IFN-γ-inducible subunits coincides with elevated expression of phospho-STAT-1 and interferon regulatory factor-1 (IRF-1). However, inhibition of NF-κB signaling in cytokine-treated astrocytes reduces IRF-1 expression without affecting that of i-20S, c-20S and PA28. This suggests that STAT-1 is capable of increasing the transcription of i20S-specific subunits and PA28α/β by itself. The lack of a decrease in proteasome β5 mRNA expression is consistent with the fact that Nrf1 (Nfe2l1) and Nrf2 (Nfe2l2) levels are not reduced by pro-inflammatory cytokines. In contrast, we previously found that there is a significant Nrf1 dysregulation and reduced β5 mRNA expression in the spinal cords of mice with experimental autoimmune encephalomyelitis (EAE). Thus, there are stressors in EAE, other than a pro-inflammatory environment, that are not present in cytokine-treated cells.
journal_name
Neurochem Resjournal_title
Neurochemical researchauthors
Shanley KL,Hu CL,Bizzozero OAdoi
10.1007/s11064-020-02958-8subject
Has Abstractpub_date
2020-04-01 00:00:00pages
860-871issue
4eissn
0364-3190issn
1573-6903pii
10.1007/s11064-020-02958-8journal_volume
45pub_type
杂志文章abstract：:Nitric oxide (NO) regulates numerous physiological process and is the main source of reactive nitrogen species (RNS). NO promotes cell survival, but it also induces apoptotic death having been involved in the pathogenesis of several neurodegenerative diseases. NO and superoxide anion react to form peroxynitrite, which...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-015-1805-9
更新日期：2016-02-01 00:00:00
abstract：:The vesicular hypothesis has stimulated fruitful investigations on many secreting systems. In the case of rapid synaptic transmission, however, the hypothesis has been found difficult to reconcile with a number of well established observations. Brief impulses of transmitter molecules (quanta) are emitted from nerve te...
journal_title：Neurochemical research
pub_type： 杂志文章,评审
doi：10.1023/a:1022451224748
更新日期：1998-05-01 00:00:00
abstract：:To evaluate the influence of light and darkness on brain pyroglutamyl-peptidase I (pGluPI) activity, four experimental groups of rats were compared at the same time-point (10.00 h). Two groups were designed with a standard 12-12 h light-dark cycle: In group A, the lights were on from 7.00 h to 19.00 h, and the experim...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1023/a:1010996425408
更新日期：2001-05-01 00:00:00
abstract：:Heme oxygenase-1 (HO-1) plays a crucial role in oxidative stress processes, apoptosis and cell differentiation. Further, some proteins related to cell cycle including cyclins and p21 are important markers of astrocyte cultures. Aim of investigation was to study the effects of cholinergic precursors (choline, CDP-choli...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-012-0873-3
更新日期：2012-12-01 00:00:00
abstract：:Plasmalogens are ether-linked phospholipids that are abundant in nervous tissues. Their biological role is unclear, but may involve membrane structure/function and antioxidant activities. This study further investigates a recent report that chronic administration of myo-inositol in rats increased brain phosphatidyleth...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1023/b:nere.0000018859.23881.f9
更新日期：2004-04-01 00:00:00
abstract：:More than a decade has passed since Zic family zinc finger proteins were discovered to be transcription factors controlling neuroectodermal differentiation (neural induction) in Xenopus laevis embryos. Although BMP-signal blocking has been shown to be a major upregulator of Zic genes in neuroectodermal differentiation...
journal_title：Neurochemical research
pub_type： 杂志文章,评审
doi：10.1007/s11064-011-0422-5
更新日期：2011-07-01 00:00:00
abstract：:The pineal gland is a neuroendocrine gland responsible for nocturnal synthesis of melatonin. During early development of the rodent pineal gland from the roof of the diencephalon, homeobox genes of the orthodenticle homeobox (Otx)- and paired box (Pax)-families are expressed and are essential for normal pineal develop...
journal_title：Neurochemical research
pub_type： 杂志文章,评审
doi：10.1007/s11064-012-0906-y
更新日期：2013-06-01 00:00:00
abstract：:Excitatory and inhibitory neurotransmission in the central nervous system can be modulated by neurosteroids. We previously found that in rat hippocampal slices allopregnanolone (3α-hydroxy-5α-pregnan-20-one), a positive GABA(A) receptor modulator, suppresses the epileptic discharges induced by 4-aminopyridine (4-AP), ...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-011-0649-1
更新日期：2012-03-01 00:00:00
abstract：:Using a model of middle cerebral artery occlusion (MCAO), we have previously demonstrated that treadmill exercise promotes angiogenesis in the ischemic penumbra through caveolin-1/VEGF signaling pathways. However, the function of caveolin-1/VEGF signaling in neurogenesis after MCAO has not been determined. In this stu...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-016-2081-z
更新日期：2017-02-01 00:00:00
abstract：:Alzheimer's disease is a neurodegenerative disease characterized by the production of β-amyloid proteins and hyperphosphorylation of tau protein. Inflammation and apoptotic severity were highly correlated with earlier age at onset of Alzheimer's disease and were also associated with cognitive decline. This study aims ...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-012-0866-2
更新日期：2012-12-01 00:00:00
abstract：:It is thought that changes in gene expression in the brain mediate chronic ethanol-induced complex behaviors such as tolerance, dependence, and sensitization, and also relate to ethanol-induced brain toxicity. Using high-density filter-based cDNA microarrays (GeneFilters), we analyzed the expression of over 5000 genes...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1023/a:1020937728506
更新日期：2002-10-01 00:00:00
abstract：:The release of labeled norepinephrine from preloaded synaptosomes requires the presence of potassium and calcium. ATP-dependent binding of calcium to synaptic plasma membranes (SPM) may provide a means of maintaining the cation in a readily available pool for the triggering of transmitter release. A high Ca-binding ca...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00965106
更新日期：1983-04-01 00:00:00
abstract：:"Kindling" is a phenomenon of epileptogenesis, which has been widely used as an experimental model of temporal lobe epilepsy. At the present work we investigated the contribution of NMDA receptors in the Pentylenetetrazol-induced "kindling" model in the mouse brain, by using quantitative autoradiography and the radioa...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1023/a:1021143813935
更新日期：1999-12-01 00:00:00
abstract：:Ethyl pyruvate (EP) has recently been reported to afford protection against neurodegenerative disorders. However, the mechanism underlying EP-mediated neuroprotection remains to be elucidated. Because peroxynitrite has been extensively implicated in the pathogenesis of various forms of neurodegenerative disorders via ...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-009-0059-9
更新日期：2010-02-01 00:00:00
abstract：:The presynaptic dopamine (DA) D2 receptor-mediated regulation of ATP-sensitive potassium (K+ATP) channels was examined in slices of the rat caudate-putamen. When slices were incubated with the specific D2 receptor antagonist (-)-sulpiride (SLP), a concentration-dependent increase of extracellular DA release was observ...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1023/a:1026620316090
更新日期：2000-12-01 00:00:00
abstract：:Two major glycoproteins, P0 and PASII/PMP22, are specifically expressed in peripheral myelin. Point mutations of these proteins and over or under expression of PASII/PMP22 cause various hereditary peripheral neuropathies. P0 is well characterized as a major adhesion molecule in PNS myelin, but the function of PASII/PM...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1023/a:1010927001378
更新日期：2001-06-01 00:00:00
abstract：:Memory impairment is the most common symptom in patients with Alzheimer's disease. The purpose of this study is to evaluate the memory enhancing effects of P7C3, a recently identified compound with robust proneurogenic and neuroprotective effects, on the cognitive impairment induced by scopolamine, a muscarinic acetyl...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-015-1783-y
更新日期：2016-05-01 00:00:00
abstract：:Incubation of [3H]-tyrosine methionine5-enkephalin (MET) with human brain preparations (100,000g supernatant; sections of the limbic system, thalamus, basal ganglia, cerebellum, and cortex) results in its rapid and complete degradation; over 95% of the initial labeled tyrosine is recovered as the free aminoacid within...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-007-9418-6
更新日期：2008-01-01 00:00:00
abstract：:The aggravated risk on intracerebral hemorrhage (ICH) with drugs used for stroke patients should be estimated carefully. We therefore established sensitive quantification methods and provided a rat ICH model for detection of ICH deterioration. In ICH intrastriatally induced by 0.014-unit, 0.070-unit, and 0.350-unit co...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1023/a:1022826220469
更新日期：2003-05-01 00:00:00
abstract：:The effect of ageing and the relationships between the catalytic properties of enzymes linked to Krebs' cycle, electron transfer chain, glutamate and aminoacid metabolism of cerebral cortex, a functional area very sensitive to both age and ischemia, were studied on mitochondria of adult and aged rats, after complete i...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-009-0004-y
更新日期：2009-12-01 00:00:00
abstract：:Addiction is a debilitating, chronic psychiatric disorder that is difficult to cure completely owing to the high rate of relapse. Behavioral sensitization is considered to may underlie behavioral changes, such as relapse, caused by chronic abuse of psychomotor stimulants. Thus, its animal models have been widely used ...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-016-1976-z
更新日期：2016-10-01 00:00:00
abstract：:The aim of present study was to determine the effect of 3',4'-dihydroxyflavonol (DiOHF) on lipid peroxidation in experimental brain ischemia-reperfusion in rats. Present study was performed on the 34 male Wistar-albino rats, weigth 350-400 g. Experiment groups were designed as 1-Sham; 2-Ischemia-reperfusion; animal we...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-016-1889-x
更新日期：2016-07-01 00:00:00
abstract：:In the experimental setting, taurine is known to be released from swollen cells to reestablish their normal volume. However, its clinical relevance has not been fully understood. This study was undertaken to reveal changes in cerebrospinal fluid (CSF) amino acids concentration in patients with severe traumatic brain i...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-004-9693-4
更新日期：2005-01-01 00:00:00
abstract：:Acidosis (pH 6.0) led to significant decrease in high-affinity choline uptake by rat brain synaptosomes. The effects persisted following pH readjustment (7.4) of the incubation medium, consisting of decrease in both Km and Vmax of the affinity system. pH readjustment coincided with synaptosomal leakage of lactate dehy...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00969699
更新日期：1995-07-01 00:00:00
abstract：:Due to the biological importance of sodium and its relative scarcity within many natural environments, 'salt appetite' has evolved whereby dietary salt is highly sought after and palatable when tasted. In addition to peripheral responses, salt depletion is detected within the brain via circumventricular organs and 11β...
journal_title：Neurochemical research
pub_type： 杂志文章,评审
doi：10.1007/s11064-017-2336-3
更新日期：2018-01-01 00:00:00
abstract：:The axonal transport of tRNA has been investigated in the chick optic system. Chicks were injected with [3H]uridine intraocularly or intracranially and the RNA of the retina, nerve complex, and tecta separated by polyacrylamide gel electrophoresis and then counted. The ratio of tRNA to rRNA specific activities increas...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00966323
更新日期：1978-08-01 00:00:00
abstract：:Acute tryptophan depletion (ATD) is extensively used to investigate the role of central serotonin (5-HT). However, several studies reported that ATD had no significant effect on central 5-HT concentration and some ATD-induced changes was independent of 5-HT in the rodent brain. Therefore, the potential mechanism of AT...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-013-1177-y
更新日期：2013-12-01 00:00:00
abstract：:The release of endogenous amino acids from depolarized rat hippocampal mossy fiber synaptosomes was investigated to assess the possible role(s) of glutamate and aspartate in mediating the excitatory mossy fiber synaptic input. The relative proportions of prodynorphin-derived peptides concomitantly released with amino ...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00969176
更新日期：1990-01-01 00:00:00
abstract：:Activation of cholinergic muscarinic receptors results in an increased turnover of membrane inositol phospholipids. In rat cerebral cortex slices, carbachol- and acetylcholine-induced inositol phosphates ([3H]InsPs) accumulation is maximal in 7 day-old rats and lowest in adults, while the density of muscarinic binding...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00969181
更新日期：1990-01-01 00:00:00
abstract：:The present work describes the kinetics of enkephalin hydrolysis by plasma enzymes and the fragmentation pattern of both the parent peptide and of the first hydrolysis by-products. The degradation kinetics were followed by positive identification of the hydrolysis fragments by chromatographic methods, by amino acid an...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00964980
更新日期：1985-10-01 00:00:00
