Abstract:
:Stromal cell derived factor-1 (SDF-1) and basic fibroblast growth factor (bFGF) were reported to induce the differentiation of bone marrow stem cells (BMSCs) into cells with characteristics of periodontal ligament fibroblasts. Thus SDF-1 and bFGF may play a positive role in BMSCs-mediated periodontal ligament regeneration. Here, the methylthiazolyldiphenyl tetrazolium bromide (MTT) assay was used to investigate the effect of scaffolds, SDF-1 and bFGF on BMSCs proliferation. RT-PCR and Western blot were used to evaluate gene and protein expression. Beagle dogs were used to establish an animal model of tooth reimplantation and to investigate the effects of scaffolds, BMSCs, SDF-1 and bFGF on periodontal ligament regeneration. X-ray images and micro computed tomography (micro CT) were used to assess morphological changes in replanted teeth and surrounding alveolar bone. H&E staining and Masson's staining were also performed. BMSCs from Beagle dogs growth on scaffolds consisted of dense structured collagens. SDF-1 and bFGF effectively promoted the differentiation of BMSCs into fibroblasts, periodontal membrane reconstruction, and cell proliferation in vitro. SDF-1 and bFGF also stimulated the expression of type I collagen (Col I), type III collagen (Col III), CXC family chemokine receptor 4 (CXCR4), and S100 calcium binding protein A4 (S100A4), and decreased the expression of alkaline phosphatase (ALP). In our experimental Beagle dog model of tooth extraction and replantation, application of SDF-1 and bFGF significantly elevated periodontal membrane reconstruction and thus supported the survival of replanted teeth. In conclusion, the findings from the present study demonstrated that SDF-1 and bFGF enhance the process of periodontal ligament reconstruction, and provide a basis and reference for the use of stem cell tissue engineering in promoting periodontal membrane regeneration.
journal_name
Biosci Repjournal_title
Bioscience reportsauthors
Xu M,Wei X,Fang J,Xiao Ldoi
10.1042/BSR20190785subject
Has Abstractpub_date
2019-12-20 00:00:00issue
12eissn
0144-8463issn
1573-4935pii
221377journal_volume
39pub_type
杂志文章abstract::Atherosclerosis is the primary cause of several cardiovascular diseases. Oxidized low-density lipoprotein (ox-LDL)-induced apoptosis, endothelial-mesenchymal transition (EndMT), and inflammation are crucial for the progression of cardiovascular diseases, including atherosclerosis. Naringin, a major compound from tomat...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20193431
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journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01119894
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abstract::Pulmonary arterial hypertension (PAH) is a life-threatening chronic cardiopulmonary disorder. However, studies providing PAH-related gene expression profiles are scarce. To identify hub genes involved in PAH, we investigate two microarray data sets from gene expression omnibus (GEO). A total of 150 differentially expr...
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journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01122726
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journal_title:Bioscience reports
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journal_title:Bioscience reports
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doi:10.1042/BSR20171056
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journal_title:Bioscience reports
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doi:10.1007/BF01855015
更新日期:1996-08-01 00:00:00
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journal_title:Bioscience reports
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doi:10.1042/BSR20193845
更新日期:2020-02-28 00:00:00
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journal_title:Bioscience reports
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doi:10.1042/BSR20182196
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journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01128815
更新日期:1984-09-01 00:00:00
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journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20170289
更新日期:2017-06-21 00:00:00
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journal_title:Bioscience reports
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doi:10.1042/BSR20170770
更新日期:2019-08-23 00:00:00
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journal_title:Bioscience reports
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doi:10.1007/BF01114759
更新日期:1986-07-01 00:00:00
abstract:BACKGROUND/AIMS:MicroRNAs (miRNAs) are involved in the pathogenesis of osteoarthritis (OA). The present study aimed to investigate the potential function of miR-122 in the development of OA and its potential molecular mechanisms. METHODS:The expression of miR-122, silent information regulator 1 (SIRT1), collagen II, a...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20191908
更新日期:2020-06-26 00:00:00
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journal_title:Bioscience reports
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doi:10.1042/BSR20192712
更新日期:2020-02-28 00:00:00
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journal_title:Bioscience reports
pub_type: 杂志文章,评审
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journal_title:Bioscience reports
pub_type: 杂志文章,meta分析
doi:10.1042/BSR20171626
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journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01200213
更新日期:1995-02-01 00:00:00
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journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01118600
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journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20080032
更新日期:2008-12-01 00:00:00
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journal_title:Bioscience reports
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doi:10.1007/s10540-005-2739-2
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journal_title:Bioscience reports
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doi:10.1042/BSR20170695
更新日期:2017-07-27 00:00:00
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journal_title:Bioscience reports
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doi:10.1042/BSR20180268
更新日期:2019-05-21 00:00:00
abstract::Thrombospondins (TSPs) are evolutionarily-conserved, secreted glycoproteins that interact with cell surfaces and extracellular matrix (ECM) and have complex roles in cell interactions. Unlike the structural components of the ECM that form networks or fibrils, TSPs are deposited into ECM as arrays of nanoscale puncta. ...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20140168
更新日期:2015-05-20 00:00:00
abstract::31p nuclear magnetic resonance (NMR) spectra have been obtained from animal and human tumours grown in laboratory rodents. The tumour cells are only slightly more acid than the surrounding muscle but they tend to have large Pi and sugar phosphate peaks, suggesting anoxia, and large but variable phosphodiester peaks. T...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01114835
更新日期:1982-09-01 00:00:00
abstract:BACKGROUND:This research aimed to study the associations between XPD (G751A, rs13181), hOGG1 (C326G, rs1052133) and XRCC4 (G1394T, rs6869366) gene polymorphisms and the risk of colorectal cancer (CRC) in a Chinese Han population. METHOD:A total of 225 Chinese Han patients with CRC were selected as the study group, and...
journal_title:Bioscience reports
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doi:10.1042/BSR20181074
更新日期:2019-01-15 00:00:00