Combination of SDF-1 and bFGF promotes bone marrow stem cell-mediated periodontal ligament regeneration.

Abstract:

:Stromal cell derived factor-1 (SDF-1) and basic fibroblast growth factor (bFGF) were reported to induce the differentiation of bone marrow stem cells (BMSCs) into cells with characteristics of periodontal ligament fibroblasts. Thus SDF-1 and bFGF may play a positive role in BMSCs-mediated periodontal ligament regeneration. Here, the methylthiazolyldiphenyl tetrazolium bromide (MTT) assay was used to investigate the effect of scaffolds, SDF-1 and bFGF on BMSCs proliferation. RT-PCR and Western blot were used to evaluate gene and protein expression. Beagle dogs were used to establish an animal model of tooth reimplantation and to investigate the effects of scaffolds, BMSCs, SDF-1 and bFGF on periodontal ligament regeneration. X-ray images and micro computed tomography (micro CT) were used to assess morphological changes in replanted teeth and surrounding alveolar bone. H&E staining and Masson's staining were also performed. BMSCs from Beagle dogs growth on scaffolds consisted of dense structured collagens. SDF-1 and bFGF effectively promoted the differentiation of BMSCs into fibroblasts, periodontal membrane reconstruction, and cell proliferation in vitro. SDF-1 and bFGF also stimulated the expression of type I collagen (Col I), type III collagen (Col III), CXC family chemokine receptor 4 (CXCR4), and S100 calcium binding protein A4 (S100A4), and decreased the expression of alkaline phosphatase (ALP). In our experimental Beagle dog model of tooth extraction and replantation, application of SDF-1 and bFGF significantly elevated periodontal membrane reconstruction and thus supported the survival of replanted teeth. In conclusion, the findings from the present study demonstrated that SDF-1 and bFGF enhance the process of periodontal ligament reconstruction, and provide a basis and reference for the use of stem cell tissue engineering in promoting periodontal membrane regeneration.

journal_name

Biosci Rep

journal_title

Bioscience reports

authors

Xu M,Wei X,Fang J,Xiao L

doi

10.1042/BSR20190785

subject

Has Abstract

pub_date

2019-12-20 00:00:00

issue

12

eissn

0144-8463

issn

1573-4935

pii

221377

journal_volume

39

pub_type

杂志文章
  • Naringin protects endothelial cells from apoptosis and inflammation by regulating the Hippo-YAP Pathway.

    abstract::Atherosclerosis is the primary cause of several cardiovascular diseases. Oxidized low-density lipoprotein (ox-LDL)-induced apoptosis, endothelial-mesenchymal transition (EndMT), and inflammation are crucial for the progression of cardiovascular diseases, including atherosclerosis. Naringin, a major compound from tomat...

    journal_title:Bioscience reports

    pub_type: 杂志文章

    doi:10.1042/BSR20193431

    authors: Zhao H,Liu M,Liu H,Suo R,Lu C

    更新日期:2020-03-27 00:00:00

  • C1r serine proteinase of human complement: a case of intramolecular autolytic activation.

    abstract::This paper presents a short review of our contribution to the knowledge of the structure and function of human C1r, the activation unit of C1, the first component of the classical pathway of complement. On the basis of the domain structure of C1r, a model accounting for its autolytic activation mechanism is proposed. ...

    journal_title:Bioscience reports

    pub_type: 杂志文章

    doi:10.1007/BF01119894

    authors: Arlaud GJ,Colomb MG,Villiers CL

    更新日期:1985-10-01 00:00:00

  • Identification of novel biomarkers involved in pulmonary arterial hypertension based on multiple-microarray analysis.

    abstract::Pulmonary arterial hypertension (PAH) is a life-threatening chronic cardiopulmonary disorder. However, studies providing PAH-related gene expression profiles are scarce. To identify hub genes involved in PAH, we investigate two microarray data sets from gene expression omnibus (GEO). A total of 150 differentially expr...

    journal_title:Bioscience reports

    pub_type: 杂志文章

    doi:10.1042/BSR20202346

    authors: Ma Y,Chen SS,Feng YY,Wang HL

    更新日期:2020-09-30 00:00:00

  • The biological properties of bovine parathyroid hormone (1-41), a fragment generated from the native hormone by human leukocytes.

    abstract::Native bovine parathyroid hormone (bPTH) was found to be readily cleaved with human leukocyte elastase to yield the fragments bPTH(1-41) and bPTH(42-84). These were then isolated by reverse-phase HPLC and characterised by gas-phase sequencing and amino acid analysis. The biological activities of these fragments were a...

    journal_title:Bioscience reports

    pub_type: 杂志文章

    doi:10.1007/BF01122726

    authors: Barling PM,Lowe C

    更新日期:1987-01-01 00:00:00

  • Epithelial barrier function properties of the 16HBE14o- human bronchial epithelial cell culture model.

    abstract::The human bronchial epithelial cell line, 16HBE14o- (16HBE), is widely used as a model for respiratory epithelial diseases and barrier function. During differentiation, transepithelial electrical resistance (TER) increased to approximately 800 Ohms × cm2, while 14C-d-mannitol flux rates (Jm) simultaneously decreased. ...

    journal_title:Bioscience reports

    pub_type: 杂志文章

    doi:10.1042/BSR20201532

    authors: Callaghan PJ,Ferrick B,Rybakovsky E,Thomas S,Mullin JM

    更新日期:2020-10-30 00:00:00

  • An epithelial-mesenchymal transition-related long noncoding RNA signature correlates with the prognosis and progression in patients with bladder cancer.

    abstract::Bladder cancer is a common malignant tumour worldwide. Epithelial-mesenchymal transition (EMT)-related biomarkers can be used for early diagnosis and prognosis of cancer patients. To explore, accurate prediction models are essential to the diagnosis and treatment for bladder cancer. In the present study, an EMT-relate...

    journal_title:Bioscience reports

    pub_type: 杂志文章

    doi:10.1042/BSR20203944

    authors: Tong H,Li T,Gao S,Yin H,Cao H,He W

    更新日期:2021-01-29 00:00:00

  • IL-21 gene rs6822844 polymorphism and rheumatoid arthritis susceptibility.

    abstract::Interleukin-21 (IL-21) is a cytokine that plays a crucial role in pathogenesis and activity of the rheumatoid arthritis (RA). Meanwhile, genetic polymorphisms in the IL-21 gene may alter its expression. Previous studies have reported conflicting results assessing the association between the IL-21 rs6822844 G/T polymor...

    journal_title:Bioscience reports

    pub_type: 杂志文章

    doi:10.1042/BSR20191449

    authors: Yu M,Hou J,Zheng M,Cao Y,Alike Y,Mi Y,Zhu J

    更新日期:2020-01-31 00:00:00

  • Excessive glucocorticoid-induced muscle MuRF1 overexpression is independent of Akt/FoXO1 pathway.

    abstract::The ubiquitin-proteasome system (UPS)-dependent proteolysis plays a major role in the muscle catabolic action of glucocorticoids (GCs). Atrogin-1 and muscle-specific RING finger protein 1 (MuRF1), two E3 ubiquitin ligases, are uniquely expressed in muscle. It has been previously demonstrated that GC treatment induced ...

    journal_title:Bioscience reports

    pub_type: 杂志文章

    doi:10.1042/BSR20171056

    authors: Wang XJ,Xiao JJ,Liu L,Jiao HC,Lin H

    更新日期:2017-11-17 00:00:00

  • Modulation of the junctional integrity by low or high concentrations of cytochalasin B and dihydrocytochalasin B is associated with distinct changes in F-actin and ZO-1.

    abstract::In a study of Necturus gallbladder epithelium Benzel et al. (Benzel et al., 1980) found that low (0.2-1.2 microM) and higher concentrations (1.5 microM and more) of cytochalasin B (CB) caused an increase and decrease in the transepithelial electrical resistance (TER), respectively. Moreover, there were slight changes ...

    journal_title:Bioscience reports

    pub_type: 杂志文章

    doi:10.1007/BF01855015

    authors: Nybom P,Magnusson KE

    更新日期:1996-08-01 00:00:00

  • The relationship between intestinal goblet cells and the immune response.

    abstract::Goblet cells (GCs) are single-cell glands that produce and secrete mucin. Mucin forms a mucus layer, which can separate the materials in cavities from the intestinal epithelium and prevent the invasion of pathogenic microorganisms in various ways. GCs can also participate in the immune response through nonspecific end...

    journal_title:Bioscience reports

    pub_type: 杂志文章

    doi:10.1042/BSR20201471

    authors: Zhang M,Wu C

    更新日期:2020-10-30 00:00:00

  • Pharmacological insights into Merremia vitifolia (Burm.f.) Hallier f. leaf for its antioxidant, thrombolytic, anti-arthritic and anti-nociceptive potential.

    abstract::Merremia vitifolia (Burm.f.) Hallier f., an ethnomedicinally important plant, used in the tribal areas to treat various ailments including fever, headache, eye inflammation, rheumatism, dysentery, jaundice and urinary diseases. The present study explored the biological efficacy of the aqueous fraction of M. vitifolia ...

    journal_title:Bioscience reports

    pub_type: 杂志文章

    doi:10.1042/BSR20203022

    authors: Akter S,Jahan I,Khatun MR,Khan MF,Arshad L,Jakaria M,Haque MA

    更新日期:2021-01-29 00:00:00

  • Couplet medicines of leech and centipede granules improve erectile dysfunction via inactivation of the CaSR/PLC/PKC signaling in streptozotocin-induced diabetic rats.

    abstract::Erectile dysfunction (ED) is one of the significant complications of diabetes mellitus (DM), and CASR plays an important role in cellular antiapoptosis and NO production in the vascular endothelium by activating PKC. The present study was aimed to investigate the efficacy of Leech and Centipede Granules (LCG) through ...

    journal_title:Bioscience reports

    pub_type: 杂志文章

    doi:10.1042/BSR20193845

    authors: Ma JX,Wang B,Ding CF,Li HS,Jiang XJ,Wang CY,Yu J,Chen WQ

    更新日期:2020-02-28 00:00:00

  • microRNA-613 exerts anti-angiogenic effect on nasopharyngeal carcinoma cells through inactivating the AKT signaling pathway by down-regulating FN1.

    abstract::Background: Nasopharyngeal carcinoma (NPC) is a disease highly sensitive to radiotherapy with the unclear etiology. However, the specific effects of microRNA-613 (miR-613) on NPC still remain elusive. Therefore, the present study probes into the underlying mechanism of miR-613 in NPC via AKT signaling pathway by regul...

    journal_title:Bioscience reports

    pub_type: 杂志文章

    doi:10.1042/BSR20182196

    authors: Gao R,Feng Q,Tan G

    更新日期:2019-07-10 00:00:00

  • Cryptic I antigen activity and Mycoplasma pneumoniae-receptor activity associated with sialoglycoprotein GP-2 of bovine erythrocyte membranes.

    abstract::The 250-kDa sialoglycoprotein of bovine erythrocyte membranes, GP-2, has been found to be an exceptionally rich source of branched sialo-oligosaccharides of poly-N-acetyllactosamine (I antigen) type with receptor activity for the human pathogen Mycoplasma pneumoniae. Desialylated GP-2 is the most potent I-active subst...

    journal_title:Bioscience reports

    pub_type: 杂志文章

    doi:10.1007/BF01128815

    authors: Feizi T,Gooi HC,Loomes LM,Suzuki Y,Suzuki T,Matsumoto M

    更新日期:1984-09-01 00:00:00

  • Effect of rivaroxaban on preventing deep vein thrombosis in aged diabetics with femoral neck fractures after hip replacement.

    abstract::The present study estimates the effect of rivaroxaban on preventing deep vein thrombosis (DVT) in aged diabetics with femoral neck fractures after hip replacement. Our study consisted of 236 aged diabetics with femoral neck fractures, which were divided into the rivaroxaban and control groups. Reaction time (R time), ...

    journal_title:Bioscience reports

    pub_type: 杂志文章

    doi:10.1042/BSR20170289

    authors: Zhang YM,Jiang X,Sun YS

    更新日期:2017-06-21 00:00:00

  • Ambra1 induces autophagy and desensitizes human prostate cancer cells to cisplatin.

    abstract::Prostate cancer (PCa), the second most mortal cancer from developed countries, presents a high level of chemoresistance. There is emerging evidence underscores the critical role of autophagy in the onset, progression, and chemoresistance of PCa. In the present study, we investigated the possible role of a novel autoph...

    journal_title:Bioscience reports

    pub_type: 杂志文章

    doi:10.1042/BSR20170770

    authors: Liu J,Chen Z,Guo J,Wang L,Liu X

    更新日期:2019-08-23 00:00:00

  • Virus replication inhibitory peptide inhibits the conversion of phospholipid bilayers to the hexagonal phase.

    abstract::Virus replication inhibitory peptide (carbobenzoxy-D-Phe-L-PheGly) was shown to be a potent specific inhibitor of the replication of paramyxovirus and myxovirus (Richardson, Scheid and Choppin (1980), Virology 105, 205-222). This peptide inhibits the membrane fusing activity of a viral glycoprotein. Many agents which ...

    journal_title:Bioscience reports

    pub_type: 杂志文章

    doi:10.1007/BF01114759

    authors: Epand RM

    更新日期:1986-07-01 00:00:00

  • miR-122/SIRT1 axis regulates chondrocyte extracellular matrix degradation in osteoarthritis.

    abstract:BACKGROUND/AIMS:MicroRNAs (miRNAs) are involved in the pathogenesis of osteoarthritis (OA). The present study aimed to investigate the potential function of miR-122 in the development of OA and its potential molecular mechanisms. METHODS:The expression of miR-122, silent information regulator 1 (SIRT1), collagen II, a...

    journal_title:Bioscience reports

    pub_type: 杂志文章

    doi:10.1042/BSR20191908

    authors: Bai Y,Chen K,Zhan J,Wu M

    更新日期:2020-06-26 00:00:00

  • Prefoldin subunits (PFDN1-6) serve as poor prognostic markers in gastric cancer.

    abstract::Prefoldin subunits (PFDN), primarily known for co-chaperone function associated with cytoskeletal rearrangement, have been found involved in epithelial-mesenchymal transition (EMT) and cancer progression. However, studies focusing on the roles of PFDN in gastric cancer (GC) remain limited. The present study aims to ev...

    journal_title:Bioscience reports

    pub_type: 杂志文章

    doi:10.1042/BSR20192712

    authors: Yesseyeva G,Aikemu B,Hong H,Yu C,Dong F,Sun J,Zang L,Zheng M,Ma J

    更新日期:2020-02-28 00:00:00

  • Alternative oxidase and uncoupling protein: thermogenesis versus cell energy balance.

    abstract::The physiological role of an alternative oxidase and an uncoupling protein in plant and protists is discussed in terms of thermogenesis and energy metabolism balance in the cell. It is concluded that thermogenesis is restricted not only by a lower-limit size but also by a kinetically-limited stimulation of the mitocho...

    journal_title:Bioscience reports

    pub_type: 杂志文章,评审

    doi:10.1023/a:1013656510245

    authors: Jarmuszkiewicz W,Sluse-Goffart CM,Vercesi AE,Sluse FE

    更新日期:2001-04-01 00:00:00

  • Macrophage migration inhibitory factor-794 CATT microsatellite polymorphism and risk of tuberculosis: a meta-analysis.

    abstract::Tuberculosis (TB) is a chronic infectious disease that has been threatening public health for many years. Several studies have shown the relationship between the macrophage migration inhibitory factor (MIF)-794 CATT (MIF-794 CATT) microsatellite polymorphism and susceptibility to TB. However, the results remain inconc...

    journal_title:Bioscience reports

    pub_type: 杂志文章,meta分析

    doi:10.1042/BSR20171626

    authors: Ma M,Tao L,Liu A,Liang Z,Yang J,Peng Y,Dai X,Bai R,Ji Z,Jian M,Bao F

    更新日期:2018-07-06 00:00:00

  • Evidence that protein kinase C and mitogen activated protein kinase are not involved in the mechanism by which insulin stimulates translation in L6 myoblasts.

    abstract::Insulin stimulated a concentration-dependent increase in protein synthesis in L6 myoblasts which was significant at 1 nM. This response was not prevented by the transcription inhibitor, actinomycin D. The protein kinase C (PKC) inhibitor, Ro-31-8220, and downregulation of PKC by prolonged incubation of cells with 12-O...

    journal_title:Bioscience reports

    pub_type: 杂志文章

    doi:10.1007/BF01200213

    authors: Thompson MG,Pascal M,Mackie SC,Thom A,Morrison KS,Backwell FR,Palmer RM

    更新日期:1995-02-01 00:00:00

  • Antidepressants and brain respiration.

    abstract::Imipramine and clorgyline, at concentrations of 0.002 M, inhibit the respiration of brain tissue by 82 and 71 per cent respectively, while chloropromazine and tranylcypromine, at concentrations of 0.01 M, inhibit it about 25 per cent. Deprenyl and amphetamine at a concentration of 0.002 M inhibit brain tissue respirat...

    journal_title:Bioscience reports

    pub_type: 杂志文章

    doi:10.1007/BF01118600

    authors: Nag M,Nandi N

    更新日期:1991-02-01 00:00:00

  • Silencing of MBD1 and MeCP2 in prostate-cancer-derived PC3 cells produces differential gene expression profiles and cellular phenotypes.

    abstract::Alterations in genomic CpG methylation patterns have been found to be associated with cell transformation and neoplasia. Although it is recognized that methylation of CpG residues negatively regulates gene expression, how the various MBPs (methyl-binding proteins) contribute to this process remains elusive. To determi...

    journal_title:Bioscience reports

    pub_type: 杂志文章

    doi:10.1042/BSR20080032

    authors: Yaqinuddin A,Abbas F,Naqvi SZ,Bashir MU,Qazi R,Qureshi SA

    更新日期:2008-12-01 00:00:00

  • Foundations of vectorial metabolism and osmochemistry.

    abstract::Chemical transformations, like osmotic translocations, are transport processes when looked at in detail. In chemiosmotic systems, the pathways of specific ligand conduction are spatially orientated through osmoenzymes and porters in which the actions of chemical group, electron and solute transfer occur as vectorial (...

    journal_title:Bioscience reports

    pub_type:

    doi:10.1007/s10540-005-2739-2

    authors: Mitchell PD

    更新日期:2004-08-01 00:00:00

  • Effects of microRNA-211 on proliferation and apoptosis of lens epithelial cells by targeting SIRT1 gene in diabetic cataract mice.

    abstract::Our study aimed at exploring the effects of miR-211 on the proliferation and apoptosis of lens epithelial cells in diabetic cataract mice by targetting NAD+-dependent histone deacetylase sirtulin 1 (SIRT1). Healthy male mice were assigned into normal and diabetic cataract groups. Blood glucose, lens turbidity, and apo...

    journal_title:Bioscience reports

    pub_type: 杂志文章

    doi:10.1042/BSR20170695

    authors: Zeng K,Feng QG,Lin BT,Ma DH,Liu CM

    更新日期:2017-07-27 00:00:00

  • The SLC34A2-ROS-HIF-1-induced up-regulation of EZH2 expression promotes proliferation and chemo-resistance to apoptosis in colorectal cancer.

    abstract::Growing evidence has uncovered that SLC34A2 plays an evident role in the progression in several types of tumors. However, the biological function and underlying molecular mechanisms of SLC34A2 remain largely unknown. Here, we indicated that SLC34A2 expression was markedly increased in SW480 and HT29 cell line cells co...

    journal_title:Bioscience reports

    pub_type: 杂志文章

    doi:10.1042/BSR20180268

    authors: Li X,Xing J,Wang H,Yu E

    更新日期:2019-05-21 00:00:00

  • Modulation of the extracellular matrix patterning of thrombospondins by actin dynamics and thrombospondin oligomer state.

    abstract::Thrombospondins (TSPs) are evolutionarily-conserved, secreted glycoproteins that interact with cell surfaces and extracellular matrix (ECM) and have complex roles in cell interactions. Unlike the structural components of the ECM that form networks or fibrils, TSPs are deposited into ECM as arrays of nanoscale puncta. ...

    journal_title:Bioscience reports

    pub_type: 杂志文章

    doi:10.1042/BSR20140168

    authors: Hellewell AL,Gong X,Schärich K,Christofidou ED,Adams JC

    更新日期:2015-05-20 00:00:00

  • NMR studies of tumours.

    abstract::31p nuclear magnetic resonance (NMR) spectra have been obtained from animal and human tumours grown in laboratory rodents. The tumour cells are only slightly more acid than the surrounding muscle but they tend to have large Pi and sugar phosphate peaks, suggesting anoxia, and large but variable phosphodiester peaks. T...

    journal_title:Bioscience reports

    pub_type: 杂志文章

    doi:10.1007/BF01114835

    authors: Griffiths JR,Iles RA

    更新日期:1982-09-01 00:00:00

  • Impact of polymorphisms in DNA repair genes XPD, hOGG1 and XRCC4 on colorectal cancer risk in a Chinese Han Population.

    abstract:BACKGROUND:This research aimed to study the associations between XPD (G751A, rs13181), hOGG1 (C326G, rs1052133) and XRCC4 (G1394T, rs6869366) gene polymorphisms and the risk of colorectal cancer (CRC) in a Chinese Han population. METHOD:A total of 225 Chinese Han patients with CRC were selected as the study group, and...

    journal_title:Bioscience reports

    pub_type: 杂志文章

    doi:10.1042/BSR20181074

    authors: Jin D,Zhang M,Hua H

    更新日期:2019-01-15 00:00:00