Abstract：
:Insulin stimulated a concentration-dependent increase in protein synthesis in L6 myoblasts which was significant at 1 nM. This response was not prevented by the transcription inhibitor, actinomycin D. The protein kinase C (PKC) inhibitor, Ro-31-8220, and downregulation of PKC by prolonged incubation of cells with 12-O-tetradecanoylphorbol-13-acetate (TPA), had no effect on the ability of insulin to stimulate protein synthesis whilst completely blocking the response to TPA. In contrast, insulin failed to enhance protein synthesis significantly in the presence of either ibuprofen, a selective cyclooxygenase inhibitor or rapamycin, an inhibitor of the 70 kDa S6 kinase. When cell extracts were prepared and assayed for total myelin basic protein kinase activity, a stimulatory effect of insulin was not observed until the concentration approached 100-fold (i.e. 100 nM) that required to elicit increases in protein synthesis. Upon fractionation on a Mono-Q column, 100 nM insulin increased the activity of 3 peaks which phosphorylated myelin basic protein. Two of these peaks were identified as the 42 and 44 kDa forms of Mitogen Activated Protein (MAP) kinase by immunoblotting. In contrast, 1 nM insulin had no effect on the activity of these peaks. The data suggest that physiologically relevant concentrations of insulin do not stimulate translation in L6 cells through either PKC or the 42/44 kDa isoforms of MAP kinase and that this response is, at least in part, mediated through the activation of the 70 kDa S6 kinase by cyclooxygenase metabolites.
journal_name
Biosci Repjournal_title
Bioscience reportsauthors
Thompson MG,Pascal M,Mackie SC,Thom A,Morrison KS,Backwell FR,Palmer RMdoi
10.1007/BF01200213subject
Has Abstractpub_date
1995-02-01 00:00:00pages
37-46issue
1eissn
0144-8463issn
1573-4935journal_volume
15pub_type
杂志文章abstract：:Two mouse monoclonal antibodies against the human complement control protein, Factor H (beta 1H), are described. The antibodies are both IgG - gamma 1 - subclass and are directed against different epitopes on the human Factor H molecule. One of the antibodies, MRC OX 24, increases the cofactor activity of Factor H in ...
journal_title：Bioscience reports
pub_type： 杂志文章
doi：10.1007/BF01120205
更新日期：1983-12-01 00:00:00
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journal_title：Bioscience reports
pub_type： 杂志文章
doi：10.1042/BSR20171245
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journal_title：Bioscience reports
pub_type： 杂志文章
doi：10.1042/BSR20201384
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journal_title：Bioscience reports
pub_type： 杂志文章
doi：10.1042/BSR20110075
更新日期：2012-06-01 00:00:00
abstract：:Background: There is a clinical need for the use of engineered adipose tissue in place of surgical reconstruction. We previously found that the external volume expansion (EVE) device increased special cell clusters in well-vascularized connective stroma during adipose regeneration. However, the origin of these cell cl...
journal_title：Bioscience reports
pub_type： 杂志文章
doi：10.1042/BSR20181776
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abstract：:In the course of evolution, mitochondria lost their independence, and mtDNA became "slave" of nDNA, depending on numerous nucleus-encoded factors for its integrity, replication and expression. Mutations in any of these factors may alter the cross-talk between the two genomes and cause diseases that affect mtDNA integr...
journal_title：Bioscience reports
pub_type： 杂志文章,评审
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journal_title：Bioscience reports
pub_type： 杂志文章
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更新日期：1982-12-01 00:00:00
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journal_title：Bioscience reports
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journal_title：Bioscience reports
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更新日期：2008-10-01 00:00:00
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journal_title：Bioscience reports
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更新日期：2019-10-30 00:00:00
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journal_title：Bioscience reports
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更新日期：2019-07-18 00:00:00
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journal_title：Bioscience reports
pub_type： 杂志文章,评审
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journal_title：Bioscience reports
pub_type： 杂志文章
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更新日期：2019-07-29 00:00:00
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journal_title：Bioscience reports
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更新日期：2019-08-13 00:00:00
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journal_title：Bioscience reports
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更新日期：2020-08-28 00:00:00
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journal_title：Bioscience reports
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doi：10.1042/BSR20160243
更新日期：2016-08-31 00:00:00
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journal_title：Bioscience reports
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doi：10.1023/a:1015540909212
更新日期：2001-12-01 00:00:00
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journal_title：Bioscience reports
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更新日期：2020-01-31 00:00:00
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journal_title：Bioscience reports
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journal_title：Bioscience reports
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journal_title：Bioscience reports
pub_type： 杂志文章,评审
doi：10.1007/BF01362501
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journal_title：Bioscience reports
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journal_title：Bioscience reports
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更新日期：2019-06-18 00:00:00
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journal_title：Bioscience reports
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doi：10.1007/BF01117435
更新日期：1985-01-01 00:00:00
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journal_title：Bioscience reports
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更新日期：2019-11-29 00:00:00
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journal_title：Bioscience reports
pub_type： 杂志文章
doi：10.1007/BF01119894
更新日期：1985-10-01 00:00:00
abstract：:The role of the topoisomerase enzyme in DNA recombination was investigated by extracting chromosomal deoxyribonucleoproteins from a variety of cultured mammalian cells and assaying for the formation of recombinant DNA structures. Although each of the crude deoxyribonucleoprotein preparations contained topoisomerase ac...
journal_title：Bioscience reports
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更新日期：1982-07-01 00:00:00
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journal_title：Bioscience reports
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journal_title：Bioscience reports
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更新日期：2020-03-27 00:00:00
abstract：:More than 60 million people in the world have been diagnosed with HIV infections since the virus was recognized as the causative agent of AIDS in the 1980s. Even though more than half of the infected patients have died, effective disease treatment and prevention measures have not been established. ART (antiretroviral ...
journal_title：Bioscience reports
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