Abstract：
:The present study was designed to investigate the protective effect of moracin on primary culture of nucleus pulposus cells in intervertebral disc and explore the underlying mechanism. Moracin treatment significantly inhibited the LPS-induced inflammatory cytokine accumulation (IL-1β, IL-6 and TNF-α) in nucleus pulposus cells. And moracin also dramatically decreased MDA activity, and increased the levels of SOD and CAT induced by LPS challenge. Moreover, the expressions of Nrf-2 and HO-1 were decreased and the protein levels of p-NF-κBp65, p-IκBα, p-smad-3 and TGF-β were increased by LPS challenge, which were significantly reversed after moracin treatments. Moracin treatments also decreased the levels of matrix degradation enzymes (MMP-3, MMP-13) as indicated by RT-PCR analysis. However, Nrf-2 knockdown abolished these protective effects of moracin. Together, our results demonstrated the ability of moracin to antagonize LPS-mediated inflammation in primary culture of nucleus pulposus in intervertebral disc by partly regulating the Nrf2/HO-1 and NF-κB/TGF-β pathway in nucleus pulposus cells.
journal_name
Biosci Repjournal_title
Bioscience reportsauthors
Gu R,Huang Z,Liu H,Qing Q,Zhuan Z,Yang L,Su Z,Huang Wdoi
10.1042/BSR20191673subject
Has Abstractpub_date
2019-12-20 00:00:00issue
12eissn
0144-8463issn
1573-4935pii
221156journal_volume
39pub_type
杂志文章abstract：:Isoliquiritigenin (ISL), a natural product isolated from licorice root, exhibits anti-gastric cancer effects. However, applications of ISL are still limited in clinical practice due to its poor bioavailability. To discovery of more effective anti-gastric cancer agents based on ISL, aldol condensation reaction was appl...
journal_title：Bioscience reports
pub_type： 杂志文章
doi：10.1042/BSR20201199
更新日期：2020-06-26 00:00:00
abstract：:Our study aimed at exploring the effects of miR-211 on the proliferation and apoptosis of lens epithelial cells in diabetic cataract mice by targetting NAD+-dependent histone deacetylase sirtulin 1 (SIRT1). Healthy male mice were assigned into normal and diabetic cataract groups. Blood glucose, lens turbidity, and apo...
journal_title：Bioscience reports
pub_type： 杂志文章
doi：10.1042/BSR20170695
更新日期：2017-07-27 00:00:00
abstract：:CXCL8, a member of CXC chemokines, was constitutively expressed in many types of human cancers, and its overexpression has been shown to play a critical role in promoting tumorigenesis. The purpose of the present study was to determine CXCL8 expression in a commercial human liver tissue microarray, and elucidate the e...
journal_title：Bioscience reports
pub_type： 杂志文章
doi：10.1042/BSR20201169
更新日期：2020-08-28 00:00:00
abstract：:The role of the topoisomerase enzyme in DNA recombination was investigated by extracting chromosomal deoxyribonucleoproteins from a variety of cultured mammalian cells and assaying for the formation of recombinant DNA structures. Although each of the crude deoxyribonucleoprotein preparations contained topoisomerase ac...
journal_title：Bioscience reports
pub_type： 杂志文章
doi：10.1007/BF01115252
更新日期：1982-07-01 00:00:00
abstract：:Keratin 18 (KRT18) has been suggested to be overexpressed in most types of human tumor, but the expression pattern of KRT18 in colorectal cancer (CRC) remained unknown. In our research, KRT18 protein expression was markedly increased in CRC cancer tissues and cell lines compared with adjacent normal colorectal tissues...
journal_title：Bioscience reports
pub_type： 杂志文章
doi：10.1042/BSR20190884
更新日期：2019-08-13 00:00:00
abstract：:Conflicting opinions were recently expressed concerning the possible effect of alpha 2-adrenergic agonists upon cyclic AMP production in pancreatic islets. In the present study, clonidine inhibited glucose-induced insulin release from rat pancreatic islets, this inhibitory effect being abolished by idazoxan. Clonidine...
journal_title：Bioscience reports
pub_type： 杂志文章
doi：10.1007/BF01122227
更新日期：1984-06-01 00:00:00
abstract：:Evidence is presented in support of a pathway in skeletal muscle of glyconeogenesis (glycogen biosynthesis de novo) from L-glutamate and related amino acids involving the enzyme phosphoenolpyruvate carboxykinase (PEP CK). In the rat hemidiaphragm in vitro, not only did L-[U]-14C]glutamate exert a glycogen-sparing acti...
journal_title：Bioscience reports
pub_type： 杂志文章
doi：10.1007/BF01117013
更新日期：1981-02-01 00:00:00
abstract：:Carnosic acid (CA), a major polyphenolic diterpene present in Rosmarinus officinalis, has been reported to have multiple functions, including antitumor activity. The MTT assay, BrdU incorporation, wound healing, and colony formation were used to detect melanoma B16F10 cell growth and proliferation. Flow cytometry was ...
journal_title：Bioscience reports
pub_type： 杂志文章
doi：10.1042/BSR20180005
更新日期：2018-07-02 00:00:00
abstract：:The effect of lysolecithin (lysophosphatidylcholine) on the relaxation of rabbit aortic strip closely resembled that produced by acetylcholine (ACh) which releases the endothelium-derived relaxing factor (EDRF). Relaxation induced by lysolecithin depended on the presence of endothelium and was inhibited by hemoglobin ...
journal_title：Bioscience reports
pub_type： 杂志文章
doi：10.1007/BF01116751
更新日期：1987-10-01 00:00:00
abstract：:Information on the prevalence and type distribution of human papillomavirus (HPV) among Malaysian women is currently limited. The present study therefore aimed to provide an updated estimate on the prevalence and type distribution of HPV among Malaysian women with and without cervical cancer. Total DNA was isolated fr...
journal_title：Bioscience reports
pub_type： 临床试验,杂志文章
doi：10.1042/BSR20171268
更新日期：2018-03-29 00:00:00
abstract：:Blood platelets have the capacity to participate in a number of physiological as well as pathological processes within the circulation. In order to evaluate their cellular reactivity a number of platelet function tests have been developed. The main in vitro function tests are assessment of aggregation and adhesion, se...
journal_title：Bioscience reports
pub_type： 杂志文章
doi：10.1007/BF01114683
更新日期：1989-06-01 00:00:00
abstract：:Cervical carcinoma is the most common gynecological cancer in women worldwide. Emerging evidence has shown that long non-coding RNAs (lncRNAs) participate in multiple biological processes of cervical carcinoma tumorigenesis. We aimed to investigate the function of a novel lncRNA RP11-284F21.9 in cervical carcinoma. We...
journal_title：Bioscience reports
pub_type： 杂志文章
doi：10.1042/BSR20200784
更新日期：2020-09-30 00:00:00
abstract：:For improving Ostwald's viscometry, which is time-consuming and requires a relatively large volume of specimen to determine viscosity, we developed a capillary microviscometric method with an appropriate mathematical model, and have compared this method with Ostwald's method. ...
journal_title：Bioscience reports
pub_type： 杂志文章
doi：10.1007/BF01121952
更新日期：1983-02-01 00:00:00
abstract：:Long non-coding RNA (lncRNA) H19 has been implicated in tumor angiogenesis. However, whether H19 regulates the progression of corneal neovascularization (CNV) is unclear. The present study aimed to determine the function of H19 in CNV and its possible molecular mechanism. Here, we found that the H19 levels were remark...
journal_title：Bioscience reports
pub_type： 杂志文章
doi：10.1042/BSR20182394
更新日期：2019-05-02 00:00:00
abstract：:Erythrocyte membrane is crucial to maintain the stability of erythrocyte structure. The membrane protein on the surface of erythrocyte membrane enables erythrocyte to have plasticity and pass through the microcirculation without being blocked or destroyed. Decreased deformability of erythrocyte membrane protein will l...
journal_title：Bioscience reports
pub_type： 杂志文章
doi：10.1042/BSR20200799
更新日期：2020-06-26 00:00:00
abstract：:Western blotting was used as a powerful alternative to immunoprecipitation for the detection of the simian virus 40 (SV40) large tumor (T) antigen. After resolution by electrophoresis on a SDS-polyacrylamide gel of a [35S]methionine labeled crude extract from SV40 infected monkey kidney cells, the separated proteins w...
journal_title：Bioscience reports
pub_type： 杂志文章
doi：10.1007/BF01117060
更新日期：1985-02-01 00:00:00
abstract：:Canstatin, the non-collagenous domain of collagen type IV α-chains, belongs to a series of collagen-derived angiogenic inhibitors. In this study, the inhibitory effect of recombinant canstatin on tumour growth was investigated using a gastric cancer xenograft model. The volume and weight of tumours in mice treated wit...
journal_title：Bioscience reports
pub_type： 杂志文章
doi：10.1042/BSR20140012
更新日期：2014-04-01 00:00:00
abstract：:To clarify the mechanism of circGOLPH3 regulation on prostate cancer cells, we performed an overexpression and interference circGOLPH3 assay in prostate cancer cells PC-3 and then evaluated cellular viability, proliferation, cell cycle, and apoptosis of prostate cancer cells by MTT, CCK8, Edu stain, TUNEL stain, and f...
journal_title：Bioscience reports
pub_type： 杂志文章
doi：10.1042/BSR20200936
更新日期：2020-12-23 00:00:00
abstract：:The nature of certain forms of memory is discussed in relation to neural networks and REM sleep. ...
journal_title：Bioscience reports
pub_type： 杂志文章
doi：10.1007/BF01117331
更新日期：1988-12-01 00:00:00
abstract：:Significant amounts of somatostatin-like immunoreactivity (SLI) were detected in the extract of a human catecholamine-secreting adrenal medullary tumour. After salt fractionation and reconstitution the major portion of SLI was purified by gel filtration and two HPLC steps; in all three systems it eluted in the positio...
journal_title：Bioscience reports
pub_type： 杂志文章
doi：10.1007/BF01116377
更新日期：1982-03-01 00:00:00
abstract：AIM:The purpose of the present study was to explore the function and mechanism of tensin 1 (TNS1) in non-small cell lung cancer (NSCLC) progression. METHODS:The expression of TNS1 in NSCLC cells and tissues was assessed by RT-PCR and Western blot. Besides, Kaplan-Meier survival analysis was recruited to explore the as...
journal_title：Bioscience reports
pub_type： 杂志文章
doi：10.1042/BSR20201539
更新日期：2021-01-29 00:00:00
abstract：:The influence of lindane upon the dynamic properties of plasma membranes from rat ventral prostate has been investigated using a fluorescence polarization technique. Preincubation with lindane decreased the fluorescence polarization in a dose dependent manner. This effect, which is associated with an increased membran...
journal_title：Bioscience reports
pub_type： 杂志文章
doi：10.1007/BF01119197
更新日期：1991-04-01 00:00:00
abstract：:As a zinc transporter, SLC39A7 (zip7) is vital in intestinal epithelial self-renewal, and recent studies suggested that SLC39A7 was related to cancer progression. Whereas, little is known about the role of SLC39A7 in gastric cancer (GC). In the present study, qRT-PCR analysis demonstrated that SLC39A7 mRNA level was i...
journal_title：Bioscience reports
pub_type： 杂志文章
doi：10.1042/BSR20200041
更新日期：2020-02-28 00:00:00
abstract：:Fasudil is a derivative of 5-isoquinoline sulfonamide, which is a Rho kinase inhibitor, a wide range of pharmacological effects. Fasudil has been shown to attenuate kidney injury caused by certain substances. In the present study, metabolomic analysis of mouse kidney tissues ultra-performance liquid chromatography/qua...
journal_title：Bioscience reports
pub_type： 杂志文章
doi：10.1042/BSR20192940
更新日期：2019-11-29 00:00:00
abstract：BACKGROUND:Glioma is the most common malignant tumor in the human central nervous system. Long noncoding RNA nuclear paraspeckle assembly transcript 1 (NEAT1) promotes oncogenesis in various tumors. In the present study, we aimed to examine the role of NEAT1 in altering the properties of gliomas. METHODS:Quantitative ...
journal_title：Bioscience reports
pub_type： 杂志文章
doi：10.1042/BSR20200767
更新日期：2021-01-04 00:00:00
abstract：:Hepatocellular carcinoma (HCC) has a high recurrence rate and poor clinical outcome after currently used therapies, including radiofrequency ablation. To explore the possible mechanisms for the relapse of HCC, in the present study we focussed on long non-coding RNA (LncRNA), which has been reported to be involved in t...
journal_title：Bioscience reports
pub_type： 杂志文章
doi：10.1042/BSR20180896
更新日期：2019-11-29 00:00:00
abstract：:Fatty-acid synthesis has been measured in vivo with 3H2O in cafeteria-fed rats exhibiting diet-induced thermogenesis. Synthesis was decreased in brown adipose tissue, the liver, white adipose tissue, and the carcass of the cafeteria-fed animals compared to rats fed the normal stock diet. Whole-body synthesis was also ...
journal_title：Bioscience reports
pub_type： 杂志文章
doi：10.1007/BF01122453
更新日期：1983-03-01 00:00:00
abstract：:The hematopoietic cell kinase (HCK), a member of the Src family protein-tyrosine kinases (SFKs), is primarily expressed in cells of the myeloid and B lymphocyte lineages. Nevertheless, the roles of HCK in glioblastoma (GBM) remain to be examined. Thus, we aimed to investigate the effects of HCK on GBM development both...
journal_title：Bioscience reports
pub_type： 杂志文章
doi：10.1042/BSR20200975
更新日期：2020-06-26 00:00:00
abstract：:In order to better understand the role of cell surface glycolipids in T lymphocyte activation, heparin was used to simultaneously modulate the expression of glycolipids and the lytic capacity of lymphocytes activated by interleukin-2. Results presented here show that heparin added at the start of a 3 day culture inhib...
journal_title：Bioscience reports
pub_type： 杂志文章
doi：10.1007/BF01115230
更新日期：1988-08-01 00:00:00
abstract：:Corneal inflammation or keratitis is a significant cause of ocular morbidity around the world. Fortunately, the majority of the cases are successfully managed with medical therapy, but the failure of therapy does occur, leading to devastating consequences of either losing the vision or the eye. This review attempts to...
journal_title：Bioscience reports
pub_type： 杂志文章,评审
doi：10.1023/a:1017939725776
更新日期：2001-08-01 00:00:00
