Abstract:
:Genetic analysis of "InochinoIchi," an exceptionally large grain rice variety, was conducted through five continuous backcrosses with Koshihikari as a recurrent parent using the large grain F3 plant in Koshihikari × Inochinoichi as a nonrecurrent parent. Thorough the F2 and all BCnF2 generations, large, medium, and small grain segregated in a 1:2:1 ratio, indicating that the large grain is controlled by a single allele. Mapping by using simple sequence repeat (SSR) and single nucleotide polymorphism (SNP) markers with small grain homozygous segregants in the F2 of Nipponbare × Inochinoichi, revealed linkage with around 7.7 Mb markers from the distal end of the short arm of chromosome 2. Whole-genome sequencing on a large grain isogenic Koshihikari (BC4F2) using next-generation sequencing (NGS) identified a single nucleotide deletion in GW2 gene, which is located 8.1 Mb from the end of chromosome 2, encoding a RING protein with E3 ubiquitin ligase activity. The GW2-integrated isogenic Koshihikari showed a 34% increase in thousand kernel weight compared to Koshihikari, while retaining a taste score of 80. We further developed a large grain/semi-dwarf isogenic Koshihikari integrated with GW2 and the semidwarfing gene d60, which was found to be localized on chromosome 2. The combined genotype secured high yielding while providing robustness to withstand climate change, which can contribute to the New Green Revolution.
journal_name
Int J Mol Scijournal_title
International journal of molecular sciencesauthors
Tomita M,Yazawa S,Uenishi Ydoi
10.3390/ijms20215442subject
Has Abstractpub_date
2019-10-31 00:00:00issue
21issn
1422-0067pii
ijms20215442journal_volume
20pub_type
杂志文章abstract::Paired box gene 3 (Pax3) and cAMP responsive element-binding protein (CREB) directly interact with the cis-acting elements on the promoter of microphthalmia-associated transcription factor isoform M (MITF-M) for transcriptional activation in the melanogenic process. Tyrosinase (Tyro) is a target gene of MITF-M, and fu...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21249631
更新日期:2020-12-17 00:00:00
abstract::With the development of molecular cloning technology and the deep understanding of antibody engineering, there are diverse bispecific antibody formats from which to choose to pursue the optimal biological activity and clinical purpose. The single-chain-based bispecific antibodies usually bridge tumor cells with immune...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms18010048
更新日期:2016-12-28 00:00:00
abstract::Neuromuscular disorders (NMDs) affect 1 in 3000 people worldwide. There are more than 150 different types of NMDs, where the common feature is the loss of muscle strength. These disorders are classified according to their neuroanatomical location, as motor neuron diseases, peripheral nerve diseases, neuromuscular junc...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms21176429
更新日期:2020-09-03 00:00:00
abstract::Fertility preservation for prepubertal boys relies exclusively on cryopreservation of immature testicular tissue (ITT) containing spermatogonia as the only cells with reproductive potential. Preclinical studies that used a nude mice model to evaluate the development of human transplanted ITT were characterized by impo...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms20235833
更新日期:2019-11-20 00:00:00
abstract::Parkinson's disease (PD) is a complex disorder underpinned by both environmental and genetic factors. The latter only began to be understood around two decades ago, but since then great inroads have rapidly been made into deconvoluting the genetic component of PD. In particular, recent large-scale projects such as gen...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms21197332
更新日期:2020-10-04 00:00:00
abstract::The clustered regularly interspaced short palindromic repeats/CRISPR-associated (CRISPR/Cas) adaptive immune system has been extensively used for gene editing, including gene deletion, insertion, and replacement in bacterial and eukaryotic cells owing to its simple, rapid, and efficient activities in unprecedented res...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms19041089
更新日期:2018-04-05 00:00:00
abstract::The human gastrointestinal tract is inhabited by many types of microbiota, including bacteria, viruses, and fungi. Dysregulations of their microenvironment are associated with various health problems, not only limited to gastrointestinal disorders, such as inflammatory bowel disease, but to impacts beyond the intestin...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms20020387
更新日期:2019-01-17 00:00:00
abstract::Citalopram is a selective serotonin reuptake inhibitor, and although widely used as an antidepressant, this drug has also demonstrated interesting repairing properties leading to motor recovery and pathology amelioration in animal models of stroke and degeneration. Here, we tested the efficacy of both 7-day and 8-week...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21145062
更新日期:2020-07-17 00:00:00
abstract::Iron is an essential mineral participating in different functions of the organism under physiological conditions. Numerous biological processes, such as oxygen and lipid metabolism, protein production, cellular respiration, and DNA synthesis, require the presence of iron, and mitochondria play an important role in the...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms21217889
更新日期:2020-10-24 00:00:00
abstract::Clinical applications of camptothecin (CPT) have been heavily hindered due to its non-targeted toxicity, active lactone ring instability, and poor water solubility. Targeted drug delivery systems may offer the possibility to overcome the above issues as reported. In this research, a series of prostate-specific membran...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms19103251
更新日期:2018-10-19 00:00:00
abstract::Scaffolds made of biodegradable biomaterials are widely used to guide bone regeneration. Commonly, in vitro assessment of scaffolds' osteogenesis potential has been performed predominantly in monoculture settings. Hence, this study evaluated the potential of an unstimulated, growth factor-free co-culture system compri...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms20051068
更新日期:2019-03-01 00:00:00
abstract::Extramammary Paget's disease (EMPD) is a rare skin cancer arising in the anogenital area. Most EMPD tumors remain dormant as in situ lesions, but the outcomes of patients with metastatic EMPD are poor because of the lack of effective systemic therapies. Nectin cell adhesion molecule 4 (NECTIN4) has attracted attention...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21165891
更新日期:2020-08-16 00:00:00
abstract::With the development of new sensitive molecular techniques, circulating cell-free tumour DNA containing mutations can be identified in the plasma of cancer patients. The applications of this technology may result in significant changes to the care and management of cancer patients. Whilst, currently, these "liquid bio...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms160614122
更新日期:2015-06-19 00:00:00
abstract::Reactive oxygen species (ROS) are highly reactive signaling molecules that maintain redox homeostasis in mammalian cells. Dysregulation of redox homeostasis under pathological conditions results in excessive generation of ROS, culminating in oxidative stress and the associated oxidative damage of cellular components. ...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms20153791
更新日期:2019-08-03 00:00:00
abstract::A systematic study of the effect of composition on the thermo-physical properties of the binary mixtures of 1-methyl-3-pentyl imidazolium hexafluorophosphate [MPI][PF(6)] with poly(ethylene glycol) (PEG) [M(w) = 400] is presented. The excess molar volume, refractive index deviation, viscosity deviation, and surface te...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms12042598
更新日期:2011-01-01 00:00:00
abstract::Cotton is one of the most economically important crops in the world, and it is exposed to various abiotic stresses during its lifecycle, especially salt stress. However, the molecular mechanisms underlying cotton tolerance to salt stress are still not fully understood due to the complex nature of salt response. Theref...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms20215244
更新日期:2019-10-23 00:00:00
abstract::The analysis of saliva as a diagnostic approach for systemic diseases was proposed just two decades ago, but recently great interest in the field has emerged because of its revolutionary potential as a liquid biopsy and its usefulness as a non-invasive sampling method. Multiple molecules isolated in saliva have been p...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms17091531
更新日期:2016-09-12 00:00:00
abstract::Spinal sarcopenia is a complex and multifactorial disorder associated with a loss of strength, increased frailty, and increased risks of fractures and falls. In addition, spinal sarcopenia has been associated with lumbar spine disorders and osteoporosis, which renders making decisions on treatment modalities difficult...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms21083010
更新日期:2020-04-24 00:00:00
abstract::The use of mesenchymal stem cells (MSC) for the treatment of cutaneous wounds is currently of enormous interest. However, the broad translation of cell therapies into clinical use is hampered by their efficacy, safety, manufacturing and cost. MSCs release a broad repertoire of trophic factors and immunomodulatory cyto...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms21197038
更新日期:2020-09-24 00:00:00
abstract::Arthritis, including osteoarthritis (OA) and rheumatoid arthritis (RA), is the leading cause of years lived with disability (YLD) worldwide. Although pain is the cardinal symptom of arthritis, which is directly related to function and quality of life, the elucidation of the mechanism underlying the pathogenesis of pai...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms21020533
更新日期:2020-01-14 00:00:00
abstract::Radiation has a wide variety of effects on the liver. Fibrosis is a concern in medical fields as one of the acute effects of high-dose irradiation, such as with cancer radiotherapies. Cancer is also an important concern following exposure to radiation. The liver has an active metabolism and reacts to radiations. In ad...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms19123855
更新日期:2018-12-03 00:00:00
abstract::Various strategies have been employed to speed tissue regeneration using bioactive molecules. Interestingly, platelet concentrates derived from a patient's own blood have been utilized as a regenerative strategy in recent years. In the present study, a novel liquid platelet formulation prepared without the use of anti...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms18020331
更新日期:2017-02-04 00:00:00
abstract::The Dunkin Hartley (DH) guinea pig is a widely used naturally occurring osteoarthritis model. The aim of this study was to provide detailed evidence of age-related changes in articular cartilage, subchondral bone mineral density, and estradiol levels. We studied the female Dunkin Hartley guinea pigs at 1, 3, 6, 9, and...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms150813578
更新日期:2014-08-05 00:00:00
abstract::We have considered the Huxley-Simmons manoeuvre. On the assumption that the quick release is an elastic process and on the basis of the isometric tension and of the stiffness of the muscle fibre we calculated that the spontaneous release of the fibre requires approximately 43 micros, which is much faster than the obse...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms10030942
更新日期:2009-03-01 00:00:00
abstract::A variety of studies have suggested that the 4b/a polymorphism in the endothelial nitric oxide synthase (eNOS) was associated with coronary artery disease (CAD) risk. However, the data remain conflicting. The aim of the present meta-analysis was to estimate the overall association between risk of CAD and eNOS 4b/a pol...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,meta分析
doi:10.3390/ijms15057987
更新日期:2014-05-07 00:00:00
abstract::As a ubiquitous secondary messenger in plant signaling systems, calcium ions (Ca2+) play essential roles in plant growth and development. Within the cellular signaling network, the accurate decoding of diverse Ca2+ signal is a fundamental molecular event. Calcium-dependent protein kinases (CDPKs), identified commonly ...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms19071900
更新日期:2018-06-28 00:00:00
abstract::An increase in intracellular Ca2+ concentration ([Ca2+]i) plays a key role in controlling endothelial functions; however, it is still unclear whether endothelial Ca2+ handling is altered by type 2 diabetes mellitus, which results in severe endothelial dysfunction. Herein, we analyzed for the first time the Ca2+ respon...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21010250
更新日期:2019-12-30 00:00:00
abstract::Epithelia act as a barrier to the external environment. The extracellular environment constantly changes, and the epithelia are required to regulate their function in accordance with the changes in the environment. It has been reported that a difference of the environment between the apical and basal sides of epitheli...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms20143513
更新日期:2019-07-17 00:00:00
abstract::Brain-derived neurotrophic factor (BDNF), a member of the neurotrophin family, is involved in multiple and fundamental functions of the central and peripheral nervous systems including sensory organs. Despite recent advances in knowledge on the functional significance of BDNF and TrkB in the regulation of the acoustic...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21165787
更新日期:2020-08-12 00:00:00
abstract::Despite the critical role of melanin in the protection of skin against UV radiation, excess production of melanin can lead to hyperpigmentation and skin cancer. Pear fruits are often used in traditional medicine for the treatment of melasma; therefore, we investigated the effects of pear extract (PE) and its component...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms18081809
更新日期:2017-08-21 00:00:00