Abstract：
:The clustered regularly interspaced short palindromic repeats/CRISPR-associated (CRISPR/Cas) adaptive immune system has been extensively used for gene editing, including gene deletion, insertion, and replacement in bacterial and eukaryotic cells owing to its simple, rapid, and efficient activities in unprecedented resolution. Furthermore, the CRISPR interference (CRISPRi) system including deactivated Cas9 (dCas9) with inactivated endonuclease activity has been further investigated for regulation of the target gene transiently or constitutively, avoiding cell death by disruption of genome. This review discusses the applications of CRISPR/Cas for genome editing in various bacterial systems and their applications. In particular, CRISPR technology has been used for the production of metabolites of high industrial significance, including biochemical, biofuel, and pharmaceutical products/precursors in bacteria. Here, we focus on methods to increase the productivity and yield/titer scan by controlling metabolic flux through individual or combinatorial use of CRISPR/Cas and CRISPRi systems with introduction of synthetic pathway in industrially common bacteria including Escherichia coli. Further, we discuss additional useful applications of the CRISPR/Cas system, including its use in functional genomics.
journal_name
Int J Mol Scijournal_title
International journal of molecular sciencesauthors
Cho S,Shin J,Cho BKdoi
10.3390/ijms19041089subject
Has Abstractpub_date
2018-04-05 00:00:00issue
4issn
1422-0067pii
ijms19041089journal_volume
19pub_type
杂志文章,评审abstract：:Marine sponges remain representative of a unique source of renewable biological materials. The demosponges of the family Ianthellidae possess chitin-based skeletons with high biomimetic potential. These three-dimensional (3D) constructs can potentially be used in tissue engineering and regenerative medicine. In this s...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms20205105
更新日期：2019-10-15 00:00:00
abstract：:The aim of the study was to verify if quantification of placental specific C19MC microRNAs in plasma exosomes would be able to differentiate during the early stages of gestation between patients subsequently developing pregnancy-related complications and women with the normal course of gestation and if this differenti...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms20122972
更新日期：2019-06-18 00:00:00
abstract：:: The highly conserved transcription factor Pax6 is involved in the development of the eyes, brain, and pancreas in vertebrates and invertebrates, whereas the additional expression pattern in other organs is still elusive. In this study, we cloned and characterized two pax6 homologs in blunt snout bream (Megalobrama a...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms20071710
更新日期：2019-04-05 00:00:00
abstract：:The dysregulation of macrophage lipid metabolism drives atherosclerosis. AMP-activated protein kinase (AMPK) is a master regulator of cellular energetics and plays essential roles regulating macrophage lipid dynamics. Here, we investigated the consequences of atherogenic lipoprotein-induced foam cell formation on down...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21239033
更新日期：2020-11-27 00:00:00
abstract：:Kentucky bluegrass (KB, Poa pratensis) is one of the most widely used cool-season turfgrass species, but it is sensitive to drought stress. Molecular studies in KB are hindered by its large and complex genome structure. In this study, a comparative transcriptomic study was conducted between a short and long period of ...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms20061289
更新日期：2019-03-14 00:00:00
abstract：:α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptors are assembled of four core subunits and several additional interacting proteins. Cystine-knot AMPA receptor-modulating proteins (CKAMPs) constitute a family of four proteins that influence the trafficking, subcellular localization and function of AM...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms20061460
更新日期：2019-03-22 00:00:00
abstract：:Rho family GTPases belong to the Ras GTPase superfamily and transduce intracellular signals known to regulate a variety of cellular processes, including cell polarity, morphogenesis, migration, apoptosis, vesicle trafficking, viral transport and cellular transformation. The three best-characterized Rho family members ...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms14047089
更新日期：2013-03-28 00:00:00
abstract：:Astaxanthin, a potent antioxidant carotenoid, plays a major role in modulating the immune response. In this study, we examined the immunomodulatory effects of astaxanthin on cytokine production in primary cultured lymphocytes both in vitro and ex vivo. Direct administration of astaxanthin (70-300 nM) did not produce c...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms17010044
更新日期：2015-12-29 00:00:00
abstract：:Self-assembled peptide nanofibers (NFs) obtained from β-sheet peptides conjugated with drugs, including antigenic peptides, have recently attracted significant attention. However, extensive studies on the interactions of β-sheet peptide NFs with model cell membranes have not been reported. In this study, we investigat...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21249518
更新日期：2020-12-14 00:00:00
abstract：:This study evaluated frequency-dependent effects of extremely low frequency electromagnetic fields (ELF-EMFs) on melanogenesis by melanocytes in vitro. Melanocytes were exposed to 2 mT EMFs at 30-75 Hz for 3 days before melanogenesis was examined. Exposure to ELF-EMFs at 50 and 60 Hz induced melanogenic maturation wit...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms18102120
更新日期：2017-10-11 00:00:00
abstract：:Glioblastoma multiforme (GBM) is the most common type of primary and malignant tumor occurring in the adult central nervous system. GBM often invades surrounding regions of the brain during its early stages, making successful treatment difficult. Osthole, an active constituent isolated from the dried C. monnieri fruit...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms15033889
更新日期：2014-03-04 00:00:00
abstract：:5-Fluorouracil (5-FU) is an important chemotherapeutic agent for the systemic treatment of colorectal cancer (CRC), but its effectiveness against CRC is limited by increased 5-FU resistance caused by the hypoxic tumor microenvironment. The purpose of our study was to assess the feasibility of using quinacrine (QC) to ...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms20184366
更新日期：2019-09-05 00:00:00
abstract：:The dysfunction of astrocytic inwardly rectifying potassium (Kir) 4.1 channels, which mediate the spatial potassium-buffering function of astrocytes, is known to be involved in the development of epilepsy. Here, we analyzed the Kir4.1 expressional changes in Leucine-Rich Glioma-Inactivated 1 (Lgi1) mutant rats, which ...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms20051013
更新日期：2019-02-26 00:00:00
abstract：:Drought adversely affects crop production across the globe. The root system immensely contributes to water management and the adaptability of plants to drought stress. In this study, drought-induced phenotypic and transcriptomic responses of two contrasting chickpea (Cicer arietinum L.) genotypes were compared at the ...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21051781
更新日期：2020-03-05 00:00:00
abstract：:Methyl CpG binding protein-2 (MeCP2) isoforms (E1 and E2) are important epigenetic regulators in brain cells. Accordingly, MeCP2 loss- or gain-of-function mutation causes neurodevelopmental disorders, including Rett syndrome (RTT), MECP2 duplication syndrome (MDS), and autism spectrum disorders (ASD). Within different...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms20081845
更新日期：2019-04-14 00:00:00
abstract：:Well-run screening programs for cervical cancer in the population at risk have been shown to result in a sharp decrease in the incidence and mortality of cervical cancer in a number of large populations. Expression patterns of a recently identified biomarker family, microRNA, appear to be characteristic of tumor type ...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms15010758
更新日期：2014-01-08 00:00:00
abstract：:The presence of CD4 T lymphocytes has been described for several teleost species, while many of the main T cell subsets have not been characterized at a cellular level, because of a lack of suitable tools for their identification, e.g., monoclonal antibodies (mAbs) against cell markers. We previously described the tis...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21114180
更新日期：2020-06-11 00:00:00
abstract：:The maintenance of ordinal cell cycle phases is a critical biological process in cancer genesis, which is a crucial target for anti-cancer drugs. As an important natural isoquinoline alkaloid from Chinese herbal medicine, Berberine (BBR) has been reported to possess anti-cancer potentiality to induce cell cycle arrest...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms19020327
更新日期：2018-01-23 00:00:00
abstract：:Glucose-6-phosphate dehydrogenase (G6PD) deficiency in humans causes severe disease, varying from mostly asymptomatic individuals to patients showing neonatal jaundice, acute hemolysis episodes or chronic nonspherocytic hemolytic anemia. In order to understand the effect of the mutations in G6PD gene function and its ...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms17050787
更新日期：2016-05-21 00:00:00
abstract：:Tauroursodeoxycholic acid (TUDCA) is a US FDA-approved hydrophilic bile acid for the treatment of chronic cholestatic liver disease. In the present study, we investigate the effects of TUDCA on the proliferation and differentiation of osteoblasts and its therapeutic effect on a mice model of osteoporosis. Following tr...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21124274
更新日期：2020-06-16 00:00:00
abstract：:n/a. ...
journal_title：International journal of molecular sciences
pub_type： 已发布勘误
doi：10.3390/ijms18010178
更新日期：2017-01-17 00:00:00
abstract：:Autophagy is a general protective mechanism for maintaining homeostasis in eukaryotic cells, regulating cellular metabolism, and promoting cell survival by degrading and recycling cellular components under stress conditions. The degradation pathway that is mediated by autophagy receptors is called selective autophagy,...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms21062008
更新日期：2020-03-16 00:00:00
abstract：:VDACs (voltage-dependent anion-selective channels) are pore-forming proteins of the outer mitochondrial membrane, whose permeability is primarily due to VDACs' presence. In higher eukaryotes, three isoforms are raised during the evolution: they have the same exon-intron organization, and the proteins show the same cha...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21197388
更新日期：2020-10-07 00:00:00
abstract：:Aquaporin-5 (AQP5) is a membrane water channel widely distributed in human tissues that was found up-regulated in different tumors and considered implicated in carcinogenesis in different organs and systems. Despite its wide distribution pattern and physiological importance, AQP5 short-term regulation was not reported...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms17122090
更新日期：2016-12-13 00:00:00
abstract：:Poplars have evolved various strategies to optimize acclimation responses to environmental conditions. However, how poplars balance growth and nitrogen deficiency remains to be elucidated. In the present study, changes in root development, carbon and nitrogen physiology, and the transcript abundance of associated gene...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms19082302
更新日期：2018-08-06 00:00:00
abstract：:Pulmonary vascular remodeling is a pathophysiological feature that common to all classes of pulmonary hypertension (PH) and right ventricular dysfunction, which is the major prognosis-limiting factor. Vascular, as well as cardiac tissue remodeling are associated with a re-expression of fetal variants of cellular adhes...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms18112371
更新日期：2017-11-08 00:00:00
abstract：:DEFECTIVE IN ANTHER DEHISCENCE 1 (DAD1), a phospholipase A1, utilizes galactolipids (18:3) to generate α-linolenic acid (ALA) in the initial step of jasmonic acid (JA) biosynthesis in Arabidopsis thaliana. In this study, we isolated the JcDAD1 gene, an ortholog of Arabidopsis DAD1 in Jatropha curcas, and found that it...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21238923
更新日期：2020-11-24 00:00:00
abstract：:Jatropha curcas is a promising renewable feedstock for biodiesel and bio-jet fuel production. To study gene expression in Jatropha in different tissues throughout development and under stress conditions, we examined a total of 11 typical candidate reference genes using real-time quantitative polymerase chain reaction ...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms141224338
更新日期：2013-12-13 00:00:00
abstract：:The WRKY web, which is comprised of a subset of WRKY transcription factors (TFs), plays a crucial role in the regulation of plant immunity, however, the mode of organization and operation of this network remains obscure, especially in non-model plants such as pepper (Capsicum annuum). Herein, CaWRKY22, a member of a s...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms19051426
更新日期：2018-05-10 00:00:00
abstract：:Next-generation sequencing (NGS) is a cost-effective technology capable of screening several genes simultaneously; however, its application in a clinical context requires an established workflow to acquire reliable sequencing results. Here, we report an optimized NGS workflow analyzing 22 lung cancer-related genes to ...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms161226129
更新日期：2015-12-03 00:00:00
