Abstract：
:Fertility preservation for prepubertal boys relies exclusively on cryopreservation of immature testicular tissue (ITT) containing spermatogonia as the only cells with reproductive potential. Preclinical studies that used a nude mice model to evaluate the development of human transplanted ITT were characterized by important spermatogonial loss. We hypothesized that the encapsulation of testicular tissue in an alginate matrix supplemented with nanoparticles containing a necrosis inhibitor (NECINH-NPS) would improve tissue integrity and germ cells' survival in grafts. We performed orthotopic autotransplantation of 1 mm³ testicular tissue fragments recovered form mice (aged 4-5 weeks). Fragments were either non-encapsulated, encapsulated in an alginate matrix, or encapsulated in an alginate matrix containing NECINH-NPs. Grafts were recovered 5- and 21-days post-transplantation. We evaluated tissue integrity (hematoxylin-eosin staining), germ cells survival (immunohistochemistry for promyelocytic leukemia zinc-finger, VASA, and protein-boule-like), apoptosis (immunohistochemistry for active-caspase 3), and lipid peroxidation (immunohistochemistry for malondialdehyde). NECINH-NPs significantly improved testicular tissue integrity and germ cells' survival after 21 days. Oxidative stress was reduced after 5 days, regardless of nanoparticle incorporation. No effect on caspase-dependent apoptosis was observed. In conclusion, NECINH-NPs in an alginate matrix significantly improved tissue integrity and germ cells' survival in grafts with the perspective of higher reproductive outcomes.
journal_name
Int J Mol Scijournal_title
International journal of molecular sciencesauthors
Del Vento F,Vermeulen M,Ucakar B,Poels J,des Rieux A,Wyns Cdoi
10.3390/ijms20235833subject
Has Abstractpub_date
2019-11-20 00:00:00issue
23issn
1422-0067pii
ijms20235833journal_volume
20pub_type
杂志文章abstract：:Osteoporosis morphology is characterized by bone resorption and decreases in micro-architecture parameters. Anti-osteoporosis therapy targets osteoclasts because bone resorption is a unique function of osteoclasts. Anti-c-fms antibodies against the receptor for macrophage colony-stimulating factor (M-CSF) inhibit oste...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21176120
更新日期：2020-08-25 00:00:00
abstract：:In vitro plant regeneration addresses basic questions of molecular reprogramming in the absence of embryonic positional cues. The process is highly dependent on the genotype and explant characteristics. However, the regulatory mechanisms operating during organ differentiation from in vitro cultures remain largely unkn...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms20092079
更新日期：2019-04-27 00:00:00
abstract：:Plant mitochondrial NAD-malic enzyme (NAD-ME), which is composed of α- and β-subunits in many species, participates in many plant biosynthetic pathways and in plant respiratory metabolism. However, little is known about the properties of woody plant NAD-MEs. In this study, we analyzed four NAD-ME genes (PtNAD-ME1 thro...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms140712994
更新日期：2013-06-24 00:00:00
abstract：:Parkinson's disease (PD) is caused by the loss of dopaminergic (DA) neurons in the midbrain substantia nigra (SN). Neuroinflammation, which is marked by microglial activation, plays a very important role in the pathogenesis of PD. Pro-inflammatory mediators produced by activated microglia could damage DA neurons. Henc...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms19010012
更新日期：2017-12-21 00:00:00
abstract：:Cypermethrin is one of the most highly effective synthetic pyrethroid insecticides. The toxicity of cypermethrin to the reproductive and nervous systems has been well studied. However, little is known about the toxic effect of cypermethrin on immune cells such as macrophages. Here, we investigated the cytotoxicity of ...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms17060885
更新日期：2016-06-17 00:00:00
abstract：:Complex neuropsychiatric-cardiac syndromes can be genetically determined. For the first time, the authors present a syndromal form of short QT syndrome in a 34-year-old German male patient with extracardiac features with predominant psychiatric manifestation, namely a severe form of secondary high-functioning autism s...
journal_title：International journal of molecular sciences
pub_type：
doi：10.3390/ijms21228611
更新日期：2020-11-15 00:00:00
abstract：:Remote ischemic conditioning (RIC) confers cardioprotection in patients with ST-segment elevation myocardial infarction (STEMI). Despite intense research, the translation of RIC into clinical practice remains a challenge. This may, at least partly, be due to confounding factors that may modify the efficacy of RIC. The...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms20133246
更新日期：2019-07-02 00:00:00
abstract：:A systematic study of the effect of composition on the thermo-physical properties of the binary mixtures of 1-methyl-3-pentyl imidazolium hexafluorophosphate [MPI][PF(6)] with poly(ethylene glycol) (PEG) [M(w) = 400] is presented. The excess molar volume, refractive index deviation, viscosity deviation, and surface te...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms12042598
更新日期：2011-01-01 00:00:00
abstract：:Understanding the importance of oral microbiota in human health and disease also leads to an expansion of the knowledge on functional, metabolic, and molecular alterations directly contributing to oral and systemic pathologies. To date, a compelling number of studies have documented the crucial role of some oral cavit...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21217914
更新日期：2020-10-24 00:00:00
abstract：:The epiphyseal plate is a hyaline cartilage plate that sits between the diaphysis and the epiphysis. The objective of this study was to determine the impact of an injury in the growth plate chondrocytes through the study of histological morphology, immunohistochemistry, histomorphometry and Western Blot analyses of th...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms140815767
更新日期：2013-07-29 00:00:00
abstract：:The iliotibial band (ITB) is a suitable scaffold for anterior cruciate ligament (ACL) reconstruction, providing a sufficient mechanical resistance to loading. Hence, ITB-derived fibroblasts attract interest for ligament tissue engineering but have so far not been characterized. This present study aimed at characterizi...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms20081972
更新日期：2019-04-22 00:00:00
abstract：:Parkinson's disease (PD) is the second most common neurodegenerative disease and although many studies have been done on this disease, the underlying mechanisms are still poorly understood and further studies are warranted. Therefore, this study identified circRNA expression profiles in the cerebral cortex (CC), hippo...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21083006
更新日期：2020-04-24 00:00:00
abstract：:Hepatic diseases, like viral hepatitis, autoimmune hepatitis, hereditary hemochromatosis, non-alcoholic fatty liver disease (NAFLD) and Wilson's disease, play an important role in the development of liver cirrhosis and, hence, hepatocellular carcinoma. In this review, the current treatment options and the molecular me...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms15057500
更新日期：2014-04-30 00:00:00
abstract：:It has been a long-standing question how DNA damage repair proceeds in a nuclear environment where DNA is packaged into chromatin. Several decades of analysis combining in vitro and in vivo studies in various model organisms ranging from yeast to human have markedly increased our understanding of the mechanisms underl...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms130911895
更新日期：2012-01-01 00:00:00
abstract：:Autophagy, a self-defense mechanism, has been found to be associated with drug resistance in hepatocellular carcinoma (HCC). Our study was designed to investigate the role and related mechanisms of autophagy in matrine-induced apoptosis in hepatoma cells of HepG2 and Bel7402. Cell apoptosis was detected by flow cytome...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms141223212
更新日期：2013-11-25 00:00:00
abstract：:Ostrinia furnacalis, is the major pest of maize causing significant yield losses. So far, many approaches have been used to increase the virulence of entomopathogenic fungal isolates. The current study is an attempt to estimate synergistic effect of Beauveria bassiana and Trichoderma asperellum in order to explore lar...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21218215
更新日期：2020-11-03 00:00:00
abstract：:A novel application for non-thermal plasma is the induction of immunogenic cancer cell death for cancer immunotherapy. Cells undergoing immunogenic death emit danger signals which facilitate anti-tumor immune responses. Although pathways leading to immunogenic cell death are not fully understood; oxidative stress is c...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms18050966
更新日期：2017-05-03 00:00:00
abstract：:The present study gives an overview of the binding energetics of the homologous heterodimers of cruzipain-chagasin based on the binding energy (ΔGb) prediction obtained with FoldX. This analysis involves a total of 70 homologous models of the cruzipain-chagasin complex which were constructed by homology from the combi...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms20061320
更新日期：2019-03-15 00:00:00
abstract：:One of the most challenging efforts in drug delivery is the targeting of the eye. The eye structure and barriers render this organ poorly permeable to drugs. Quite recently the entrance of nanoscience in ocular drug delivery has improved the penetration and half-life of drugs, especially in the anterior eye chamber, w...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms15011590
更新日期：2014-01-22 00:00:00
abstract：:Lung cancer is the leading cause of death in the world, and the most common type of lung cancer is non-small-cell lung cancer (NSCLC), accounting for 85% of lung cancer. Patients with NSCLC, when detected, are mostly in a metastatic stage, and over half of patients diagnosed with NSCLC die within one year after diagno...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21124218
更新日期：2020-06-13 00:00:00
abstract：:Unfolded protein response (UPR) is a cytoprotective mechanism that alleviates the protein-folding burden in eukaryotic organisms. Moderate activation of UPR is required for maintaining endoplasmic reticulum (ER) homeostasis and profoundly contributes to tumorigenesis. Defects in UPR signaling are implicated in the att...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms19071832
更新日期：2018-06-21 00:00:00
abstract：:The insulin-like growth factor (IGF) pathway plays an important role in several brain tumor entities. However, the lack of inhibitors crossing the blood-brain barrier remains a significant obstacle for clinical translation. Here, we targeted the IGF pathway using ceritinib, an off-target inhibitor of the IGF1 receptor...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms20174267
更新日期：2019-08-30 00:00:00
abstract：:Glucose-6-phosphate dehydrogenase (G6PD) deficiency is the most frequent human enzymopathy, affecting over 400 million people globally. Worldwide, 217 mutations have been reported at the genetic level, and only 19 have been found in Mexico. The objective of this work was to contribute to the knowledge of the function ...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21082732
更新日期：2020-04-15 00:00:00
abstract：:Diabetes mellitus (DM) is a risk factor for hepatocellular carcinoma (HCC). The purpose of this study was to investigate the impact of the disorder of glucose metabolism on the recurrence of HCC after curative treatment. Two hundred and eleven patients with HCC who received curative treatment in our hospital from 2006...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms20030605
更新日期：2019-01-30 00:00:00
abstract：:Liquid biopsy-based methods to test biomarkers (e.g., serum proteins and extracellular vesicles) may help to monitor brain tumors. In this proteomics-based study, we aimed to identify a characteristic protein fingerprint associated with central nervous system (CNS) tumors. Overall, 96 human serum samples were obtained...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21155359
更新日期：2020-07-28 00:00:00
abstract：:Glioblastoma multiforme (GBM) is the most common type of primary and malignant tumor occurring in the adult central nervous system. GBM often invades surrounding regions of the brain during its early stages, making successful treatment difficult. Osthole, an active constituent isolated from the dried C. monnieri fruit...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms15033889
更新日期：2014-03-04 00:00:00
abstract：:Mitogen-activated protein kinases (MAPKs) are key mediators of signaling in fungi, participating in the response to diverse stresses and in developmental processes. Since the precise regulation of MAPKs is fundamental for cell physiology, fungi bear dual specificity phosphatases (DUSPs) that act as MAP kinase phosphat...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms20071709
更新日期：2019-04-05 00:00:00
abstract：:We previously demonstrated that clinical administration of mobilized CD133+ bone marrow stem cells (BMSC) accelerates hepatic regeneration. Here, we investigated the potential of platelets to modulate CD133+BMSC homing to hepatic endothelial cells and sequestration to warm ischemic livers. Modulatory effects of platel...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21176431
更新日期：2020-09-03 00:00:00
abstract：:Fermentable sugars are important intermediate products in the conversion of lignocellulosic biomass to biofuels and other value-added bio-products. The main bottlenecks limiting the production of fermentable sugars from lignocellulosic biomass are the high cost and the low saccharification efficiency of degradation en...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21020371
更新日期：2020-01-07 00:00:00
abstract：:Vascular endothelial growth factor A (Vegfa) signaling regulates cardiovascular development. However, the cellular mechanisms of Vegfa signaling in early cardiogenesis remain poorly understood. The present study aimed to understand the differential functions and mechanisms of Vegfa signaling in cardiac development. A ...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms18020444
更新日期：2017-02-21 00:00:00
