Abstract:
:One of the chromosomal segments associated with the virulence of Shigella flexneri and transferred to Escherichia coli K-12 by conjugation has been shown to code for the production of aerobactin and for the synthesis of an iron-regulated 76,000-dalton (76K) outer membrane protein. Analysis of various E. coli K-12-S. flexneri transconjugants showed that the genes involved with the synthesis of aerobactin and with the production of the 76K protein were linked to the mtl region of the S. flexneri chromosome. S. flexneri itself synthesized a 76K protein in its outer membrane under iron restriction as well as traces of 81K and 74K proteins. An examination of four enteroinvasive strains of E. coli showed that each produced aerobactin and a 76K outer membrane protein during iron-restricted growth. The profile of the iron-regulated proteins expressed by the enteroinvasive strains of E. coli was virtually identical to that expressed by the laboratory-constructed E. coli K-12-S. flexneri hybrids under the same growth conditions.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Griffiths E,Stevenson P,Hale TL,Formal SBdoi
10.1128/IAI.49.1.67-71.1985subject
Has Abstractpub_date
1985-07-01 00:00:00pages
67-71issue
1eissn
0019-9567issn
1098-5522journal_volume
49pub_type
杂志文章abstract::Culture filtrate proteins from Mycobacterium tuberculosis induce protective immunity in various animal models of tuberculosis. Two molecular mass regions (6 to 10 kDa and 24 to 36 kDa) of short-term culture filtrate are preferentially recognized by Th1 cells in animal models as well as by patients with minimal disease...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.66.6.2728-2735.1998
更新日期:1998-06-01 00:00:00
abstract::FMS-like tyrosine kinase-3 ligand (Flt3L) is a dendritic cell (DC) growth and differentiation factor with potential in antitumor therapies and antibacterial immunization strategies. However, the effect of systemic Flt3L treatment on lung-protective immunity against bacterial infection is incompletely defined. Here, we...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00854-12
更新日期:2012-12-01 00:00:00
abstract::Serum amyloid A protein (SAA) elevation accompanies induction of secondary amyloidosis in mice given Mycobacterium butyricum in Freund adjuvant. The synthesis of SAA by cultured hepatocytes is induced by a macrophage-derived mediator, which has been identified as interleukin 1. In these studies, SAA synthesis has been...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.39.3.1147-1154.1983
更新日期:1983-03-01 00:00:00
abstract::Cronobacter sakazakii is an opportunistic pathogen that causes neonatal meningitis and necrotizing enterocolitis. Its interaction with intestinal epithelium is important in the pathogenesis of enteric infections. In this study, we investigated the involvement of the inv gene in the virulence of C. sakazakii ATCC 29544...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01397-13
更新日期:2014-05-01 00:00:00
abstract::The yeast form of the dimorphic fungus Histoplasma capsulatum survives within macrophages after phagocytosis. To do so, it must avoid, inhibit, or resist a variety of toxic oxygen metabolites. Using ferricytochrome c reduction to assay superoxide release, we examined the response of mouse macrophages to the yeast form...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.55.1.29-34.1987
更新日期:1987-01-01 00:00:00
abstract::Haemophilus somnus causes pneumonia, reproductive failure, infectious myocarditis, thrombotic meningoencephalitis, and other diseases in cattle. Although vasculitis is commonly seen as a result of systemic H. somnus infections, the pathogenesis of vascular damage is poorly characterized. In this study, we demonstrated...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.69.3.1650-1660.2001
更新日期:2001-03-01 00:00:00
abstract::A soluble hemagglutinin/protease produced by Vibrio cholerae, which has previously been shown to hydrolyze fibronectin and ovomucin and to cleave lactoferrin and the A subunit of the heat-labile enterotoxin of Escherichia coli, appears to be a zinc metalloendopeptidase. Both its hemagglutinative and protease functions...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.42.2.639-644.1983
更新日期:1983-11-01 00:00:00
abstract::The Yersinia high-pathogenicity island (HPI) is common to multiple virulence strategies used by Escherichia coli strains associated with urinary tract infection (UTI). Among the genes in this island are ybtP and ybtQ, encoding distinctive ATP binding cassette (ABC) proteins associated with iron(III)-yersiniabactin imp...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01211-15
更新日期:2016-04-22 00:00:00
abstract::This study has demonstrated the extreme sensitivity of Chlamydia trachomatis growing in McCoy cells to small changes in external amino acid supply. In the absence of cycloheximide, a decrease in the amino acid concentration of medium to 75% of control values was sufficient to induce the growth of enlarged chlamydial f...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.68.3.1457-1464.2000
更新日期:2000-03-01 00:00:00
abstract::Alveolar macrophages are the effector cells largely responsible for clearance of Pneumocystis carinii from the lungs. Binding of organisms to beta-glucan and mannose receptors has been shown to stimulate phagocytosis of the organisms. To further define the mechanisms used by alveolar macrophages for clearance of P. ca...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00393-07
更新日期:2007-08-01 00:00:00
abstract::In an attempt to clarify the mechanism(s) of increased susceptibility to oral infection in diabetics, we examined the levels of salivary antibacterial factors, including lysozyme, lactoperoxidase, and lactoferrin, in diabetic hamsters whose condition was induced with streptozotocin. Saliva was collected from these ham...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.48.2.389-394.1985
更新日期:1985-05-01 00:00:00
abstract::Mice injected intravenously with a commercially available extract of Coxiella burnetii prepared for use as the antigen in the complement fixation diagnostic test for Q fever were subsequently resistant to infection with Babesia microti, Babesia rodhaini, and Plasmodium vinckei petteri. The parasites appeared to die in...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.24.2.319-325.1979
更新日期:1979-05-01 00:00:00
abstract::Recently, a glycoprotein fraction, designated gp10/20, purified from Leishmania mexicana amazonensis was shown to induce a cellular immune response mediated by murine L3T4+ T lymphocytes. This fact led us to pursue further the characterization of this fraction. The present study demonstrated that gp10/20 is a degradat...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.56.7.1766-1770.1988
更新日期:1988-07-01 00:00:00
abstract::Phagocytosis of Borrelia burgdorferi by human polymorphonuclear leukocytes triggers oxygen-dependent and -independent mechanisms of potentially cidal outcome. Nevertheless, no factor or process has yet been singled out as being borreliacidal. We have studied the B. burgdorferi-killing ability of the myeloperoxidase-H2...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.66.4.1408-1412.1998
更新日期:1998-04-01 00:00:00
abstract::The obligate intracellular parasite Toxoplasma gondii can actively infect any nucleated cell type, including cells from the immune system. The rapid transfer of T. gondii from infected dendritic cells to effector natural killer (NK) cells may contribute to the parasite's sequestration and shielding from immune recogni...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00069-17
更新日期:2017-09-20 00:00:00
abstract::Streptococcus pneumoniae infection leads to multifold increases in sialyltransferase, galactosyltransferase, alpha 2-fucosyltransferase, and alpha 3-fucosyltransferase activity of rat liver. Such changes may reflect an increased demand for glycosylation of acute-phase proteins synthesized and secreted by the liver dur...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.29.1.114-118.1980
更新日期:1980-07-01 00:00:00
abstract::Salmonella infection can cause gastroenteritis in healthy individuals or a serious, systemic infection in immunocompromised patients and has a global impact. CD4 Th1 cells represent the main lymphocyte population that participates in bacterial clearance during both primary and secondary infections in mice of the H-2b ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00588-19
更新日期:2019-10-18 00:00:00
abstract::The gene encoding the 56-kDa protein of Orientia tsutsugamushi Shanxi was amplified by a nested PCR and cloned into the expression vector pQE30. The 56-kDa protein of O. tsutsugamushi Shanxi (Sxh56) was expressed as a fusion protein with the His(6)-binding protein of Escherichia coli by deleting the signal peptide-enc...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.71.8.4772-4779.2003
更新日期:2003-08-01 00:00:00
abstract::The nucleotide sequences of three previously undescribed Pseudomonas aeruginosa pilin structural genes are presented. Comparisons of deduced pilin primary structure and flanking DNA sequence allowed placement of these and six previously published sequences into one of two groups. Epitope mapping, using overlapping imm...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.62.2.371-376.1994
更新日期:1994-02-01 00:00:00
abstract::Pools of rat antiserum to Plasmodium berghei had different levels of protective activity as assessed by a passive transfer test. Preincubation of parasite inocula with an effective pool before injection did not significantly enhance protective activity. Removal of the antiserum from preincubated parasite inocula aboli...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.10.6.1213-1218.1974
更新日期:1974-12-01 00:00:00
abstract::One mechanism by which interferons (IFNs) can inhibit chlamydial infection is by the induction of the enzyme indoleamine 2,3-dioxygenase (IDO), which restricts the availability of tryptophan, which is required for chlamydial growth. Other immunomodulating agents, including interleukin-1 (IL-1), can interact synergisti...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.63.5.1870-1875.1995
更新日期:1995-05-01 00:00:00
abstract::Clostridium perfringens enterotoxin caused a 26 to 41% reduction in the rate of oxygen consumption by rat liver mitochondria when various tricarboxylic acid cycle intermediates were used as substrate. However, P/O ratios were unaltered. ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.15.3.999-1001.1977
更新日期:1977-03-01 00:00:00
abstract::Group A streptococci of M protein type 5 have been epidemiologically related to acute rheumatic fever in a number of reported outbreaks. Preliminary bacteriological evidence suggests that M5 may be an important "rheumatogenic" type in Santiago, Chile. To assess further the relationship of this streptococcal serotype t...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.38.2.573-579.1982
更新日期:1982-11-01 00:00:00
abstract::Mycobacterial growth inhibitory factor (MycoIF), found in supernatant fluids of mouse spleen cell cultures that have been stimulated in vitro with homologous antigen, inhibited the intracellular multiplication of virulent tubercle bacilli within normal mouse peritoneal macrophages in vitro. Antigenically stimulated H...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.12.4.833-840.1975
更新日期:1975-10-01 00:00:00
abstract::Phagocytosis and mechanisms of killing of Aspergillus fumigatus conidia by murine alveolar macrophages (AM), which are the main phagocytic cells of the innate immunity of the lung, were investigated. Engulfment of conidia by murine AM lasts 2 h. Killing of A. fumigatus conidia by AM begins after 6 h of phagocytosis. S...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.71.6.3034-3042.2003
更新日期:2003-06-01 00:00:00
abstract::To analyze the role of interleukin-10 (IL-10) in bacterial cerebral infections, we studied cerebral listeriosis in IL-10-deficient (IL-10(-/-)) and wild-type (WT) mice, the latter of which express high levels of IL-10 in both primary and secondary cerebral listeriosis. IL-10(-/-) mice succumbed to primary as well as s...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.69.7.4561-4571.2001
更新日期:2001-07-01 00:00:00
abstract::An inactivated oral enterotoxigenic Escherichia coli (ETEC) vaccine against ETEC diarrhea was given to 25 adult Swedish volunteers. The vaccine consisted of formalin-killed E. coli bacteria expressing the most common colonization factor antigens (CFAs), i.e., CFA/I, -II, and -IV, and recombinantly produced cholera B s...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.66.7.3311-3316.1998
更新日期:1998-07-01 00:00:00
abstract::LipL32 is the major outer membrane protein in pathogenic Leptospira. It is highly conserved throughout pathogenic species and is expressed in vivo during human infection. While these data suggest a role in pathogenesis, a function for LipL32 has not been defined. Outer membrane proteins of gram-negative bacteria are t...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01643-07
更新日期:2008-05-01 00:00:00
abstract::Extracts of specific granules and azurophil granules from human neutrophils were tested for their bactericidal activity against various lipopolysaccharide mutants of Salmonella typhimurium LT-2. Three purified granule populations, one specific and two azurophil, were obtained by isopycnic centrifugation of homogenized...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.19.1.131-137.1978
更新日期:1978-01-01 00:00:00
abstract::Babesiosis has long been recognized as an economically important disease of cattle, but only in the last 30 years has Babesia been recognized as an important pathogen in humans. Invasion of erythrocytes is an integral part of the Babesia life cycle. However, very little information is available on the molecules involv...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.73.1.649-651.2005
更新日期:2005-01-01 00:00:00