当前位置： SCI文献检索 > INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES期刊下所有文献 > In vivo and in vitro characterization of hydrophilic protein tag-fused Ralstonia eutropha polyhydroxyalkanoate synthase.

In vivo and in vitro characterization of hydrophilic protein tag-fused Ralstonia eutropha polyhydroxyalkanoate synthase.

Abstract：

:Polyhydroxyalkanoates (PHAs) are synthesized by bacteria as an intracellular storage polyester, where PHA synthase (PhaC) catalyzes the polymerization of its substrate hydroxyacyl-coenzyme A (HA-CoA) to form PHA. When PhaC is overexpressed in Escherichia coli, most PhaC protein is produced as insoluble inclusion bodies due to its low aqueous solubility. This study aimed to improve the solubility of Ralstonia eutropha PHA synthase (PhaCRe) by fusing a hydrophilic tag, glutathione S-transferase (GST), to the protein's N-terminus. In in vivo assays, the GST tag had no obvious effect on solubility and enzymatic activity of PhaCRe. However, an in vitro assay revealed that the surface of GST-fused PhaCRe (GST-PhaCRe) had increased hydrophilicity, and tended to form correct PhaCRe dimers when added to the (R)-3-hydroxybutyryl-CoA substrate. Although GST-PhaCRe displayed a long lag phase at the start of a polymerization reaction, granule-associated GST-PhaCRe showed higher catalytic activity than PhaCRe in kinetic analysis. The results are discussed in light of the dimerization mechanisms of PhaCRe.

journal_name

Int J Biol Macromol

journal_title

International journal of biological macromolecules

authors

Harada K,Nambu Y,Mizuno S,Tsuge T

doi

10.1016/j.ijbiomac.2019.07.095

subject

Has Abstract

pub_date

2019-10-01 00:00:00

pages

379-385

eissn

0141-8130

issn

1879-0003

pii

S0141-8130(19)33083-1

journal_volume

138

pub_type

杂志文章

相关文献

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES文献大全
  • A highly xyloglucan active lytic polysaccharide monooxygenase EpLPMO9A from Eupenicillium parvum 4-14 shows boosting effect on hydrolysis of complex lignocellulosic substrates.

    abstract：:The recently identified lytic polysaccharide monooxygenases (LPMOs) are important auxiliary proteins which contribute to lignocellulose biodegradation by oxidatively cleaving the glycosidic bonds in cellulose and other polysaccharides. The vast differences in terms of substrate specificity and regioselectivity within ...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2020.11.177

    authors： Shi Y,Chen K,Long L,Ding S

    更新日期：2021-01-15 00:00:00

  • Genome-wide identification and transcriptional profiling of small heat shock protein gene family under diverse abiotic stress conditions in Sorghum bicolor (L.).

    abstract：:The small heat shock proteins (sHsps/Hsp20s) are the molecular chaperones that maintain proper folding, trafficking and disaggregation of proteins under diverse abiotic stress conditions. In the present investigation, a genome-wide scan revealed the presence of a total of 47 sHsps in Sorghum bicolor (SbsHsps), distrib...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2019.10.023

    authors： Nagaraju M,Reddy PS,Kumar SA,Kumar A,Rajasheker G,Rao DM,Kavi Kishor PB

    更新日期：2020-01-01 00:00:00

  • Hexadecylphosphocholine (Miltefosine) stabilized chitosan modified Ampholipospheres as prototype co-delivery vehicle for enhanced killing of L. donovani.

    abstract：:Lipid nanoparticles are stable, biodegradable and biocompatible carriers offering excellent therapeutic efficacy. Here, a novel effort has been made to develop Miltefosine (HePC- hexadecylphosphocholine) stabilized chitosan anchored nanostructured lipid carriers (NLC) of Amphotericin B (AmB) as co-delivery vehicle to ...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2017.07.076

    authors： Tripathi P,Jaiswal AK,Dube A,Mishra PR

    更新日期：2017-12-01 00:00:00

  • Heme degradation and iron release of hemoglobin and oxidative stress of lymphocyte cells in the presence of silica nanoparticles.

    abstract：:Silica nanoparticles (SiO2 NPs) have been widely used in the medical and food sciences. However, their toxic effects against bio-macromolecules and cells are not well understood. The present study was aimed to investigate the adverse effects of fabricated SiO2 NPs on the human hemoglobin (Hb) by FTIR, CD, fluorescence...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2018.06.128

    authors： Azimipour S,Ghaedi S,Mehrabi Z,Ghasemzadeh SA,Heshmati M,Barikrow N,Attar F,Falahati M

    更新日期：2018-10-15 00:00:00

  • Physicochemical studies on peroxynitrite-modified H3 histone.

    abstract：:Histones are DNA protective proteins and may adopt different structures under nitrosative stress. Peroxynitrite is a powerful oxidant and nitrating agent and has in vivo existence. In this communication, we report effect of peroxynitrite-mediated oxidation and nitration on the structure of calf thymus H3 histone. Fine...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2009.10.009

    authors： Dixit K,Khan MA,Sharma YD,Moinuddin,Alam K

    更新日期：2010-01-01 00:00:00

  • Biosynthesis of Ag nanoparticles using isolated bacteria from contaminated sites and its application as an efficient catalyst for hydrazine electrooxidation.

    abstract：:In the present study, a bacterium resistance to heavy metals was isolated from contaminated areas. An eco-friendly and simple method was found to biosynthesis of silver nanoparticles (AgNPs) by reducing of aqueous Ag+ using the heavy metals resistance MKH1 bacterium. The biosynthesized AgNPs were characterized by UV-v...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2017.08.179

    authors： Rostami H,Khosravi F,Mohseni M,Rostami AA

    更新日期：2018-02-01 00:00:00

  • The effect of aluminum oxide on red blood cell integrity and hemoglobin structure at nanoscale.

    abstract：:Herein, we explored the interaction of Al2O3 NPs with RBCs and Hb to determine the effect of Al2O3 NPs on hemolytic activity and Hb denaturation. The percentage of hemolysis of extracts and direct contact assays triggered by Al2O3 NPs was calculated by determining supernatant Hb concentration at 540 nm. Far-UV CD and ...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2019.07.154

    authors： Kahbasi S,Samadbin M,Attar F,Heshmati M,Danaei D,Rasti B,Salihi A,Nanakali NMQ,Aziz FM,Akhtari K,Hasan A,Falahati M

    更新日期：2019-10-01 00:00:00

  • Haloadaptation: insights from comparative modeling studies of halophilic archaeal DHFRs.

    abstract：:Proteins of halophilic archaea function in high-salt concentrations that inactivate or precipitate homologous proteins from non-halophilic species. Haloadaptation and the mechanism behind the phenomenon are not yet fully understood. In order to obtain useful information, homology modeling studies of dihydrofolate redu...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2007.06.005

    authors： Kastritis PL,Papandreou NC,Hamodrakas SJ

    更新日期：2007-10-01 00:00:00

  • Rheological and controlled release properties of hydrogels based on mushroom hyperbranched polysaccharide and xanthan gum.

    abstract：:A hyperbranched polysaccharide, coded as TM3a, was extracted from the Pleurotus tuber-regium sclerotia. TM3a was hybridized with xanthan gum (XG) by chemical crosslinking using sodium trimetaphosphate (STMP) to obtain new hydrogels with self-healing and release-controlled properties. The oscillatory rheological measur...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2018.09.008

    authors： Zhang R,Tao Y,Xu W,Xiao S,Du S,Zhou Y,Hasan A

    更新日期：2018-12-01 00:00:00

  • Pasting and paste properties of waxy rice starch as affected by hydroxypropyl methylcellulose and its viscosity.

    abstract：:The objective of this study was to investigate the impacts of hydroxypropyl methylcellulose (HPMC) and its viscosity on the pasting and paste properties of waxy rice starch (WRS). HPMC with different viscosities (low, BN50; medium, BN40M; high, BN40H) but similar substituted group contents was used. Although the total...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2019.10.250

    authors： Lee H,Kim HS

    更新日期：2020-06-15 00:00:00

  • Expression and biochemical characterization of recombinant α-l-rhamnosidase r-Rha1 from Aspergillus niger JMU-TS528.

    abstract：:A putative cDNA of α-l-rhamnosidase was PCR-cloned from Aspergillus niger JMU-TS528 and further extracellular over-expressed in Pichia pastoris GS115. The activity of the recombinant α-l-rhamnosidase r-Rha1 was 711.9U/mL, eightfold higher than the native α-l-rhamnosidase from A. niger JMU-TS528. r-Rha1 is a N-glycosyl...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2015.12.093

    authors： Li L,Yu Y,Zhang X,Jiang Z,Zhu Y,Xiao A,Ni H,Chen F

    更新日期：2016-04-01 00:00:00

  • Rapid self-association of highly amyloidogenic H-fragments of insulin: Experiment and molecular dynamics simulations.

    abstract：:The so-called 'H-fragment' of insulin is an extremely amyloidogenic double chain peptide consisting of the N-terminal parts of A-chain and B-chain linked by a disulfide bond between Cys-7A and Cys-7B. Here, we conduct a detailed investigation of the self-association behavior of H-fragment monomers into amyloid-like fi...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2020.02.153

    authors： Dec R,Koliński M,Kouza M,Dzwolak W

    更新日期：2020-05-01 00:00:00

  • Temperature and salt optimization of kappa-carrageenan fractionation by DEAE-cellulose.

    abstract：:Carrageenans play an important role in many medical and food technology applications. However, the study of their properties has been hampered by a lack of well-defined samples (fractions). In this paper, a new principle is presented by which well-defined fractions can be obtained. It is based on batch adsorption to D...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/0141-8130(94)90017-5

    authors： Caram-Lelham N,Cleland RL,Sundelöf LO

    更新日期：1994-04-01 00:00:00

  • Immobilization of a novel cold active esterase onto Fe3O4∼cellulose nano-composite enhances catalytic properties.

    abstract：:A novel esterase, EstH was cloned, purified and characterized from the marine bacterium Zunongwangia sp. The purified EstH showed optimum activity at 30°C and pH 8.5 with ∼50% of original activity at 0°C. EstH was stable in high salt conditions (0-4.5M NaCl). To improve the characteristics and explore the possibilitie...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2016.03.016

    authors： Rahman MA,Culsum U,Kumar A,Gao H,Hu N

    更新日期：2016-06-01 00:00:00

  • Super and selective adsorption of cationic dyes using carboxylate-modified lignosulfonate by environmentally friendly solvent-free esterification.

    abstract：:Herein, following the strategy of sustainable, environment protection, circular economy development, carboxylate-modification lignosulfonate polymer (M-LSP) was synthesized from lignosulfonate by solvent-free esterifying with maleic anhydride (MA) by one step, and was used to remove the dyes by adsorption. FT-IR and X...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2020.05.037

    authors： Tang Y,Lin T,Ai S,Li Y,Zhou R,Peng Y

    更新日期：2020-09-15 00:00:00

  • Injectable pullulan hydrogel for the prevention of postoperative tissue adhesion.

    abstract：:Methods for reducing and preventing postoperative abdominal adhesions have been researched for decades; however, despite these efforts, the formation of postoperative peritoneal adhesions is continuously reported. Adhesions cause serious complications such as postoperative pain, intestinal obstruction, and infertility...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2016.02.026

    authors： Bang S,Lee E,Ko YG,Kim WI,Kwon OH

    更新日期：2016-06-01 00:00:00

  • Stabilizing enzymes by immobilization on bacterial spores: A review of literature.

    abstract：:The ever-increasing applications of enzymes are limited by the relatively poor performance in harsh processing conditions. As a result, there are constant innovations in immobilization protocols for improving biocatalyst activity and stability. Bacterial spores are cheap to generate and highly resistant to environment...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章,评审

    doi：10.1016/j.ijbiomac.2020.10.171

    authors： Ugwuodo CJ,Nwagu TN

    更新日期：2021-01-01 00:00:00

  • Physicochemical and functional characterization of mannan exopolysaccharide from Weissella confusa MD1 with bioactivities.

    abstract：:The present study focused on production, optimization and characterization of exopolysaccharide (EPS) from Weissella confusa MD1. The purified EPS MD1 was also evaluated for in vitro biological activities. The maximum yield of EPS (10.07 ± 0.32 g/L) was obtained with optimized culture conditions of 35 °C at pH 6.5 for...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2019.09.139

    authors： Lakra AK,Domdi L,Tilwani YM,Arul V

    更新日期：2020-01-15 00:00:00

  • The role of pH, temperature and nucleation in the formation of cholesteric liquid crystal spherulites from chitin and chitosan.

    abstract：:The alpha chitin and chitosan used in the experiments came from crab shell waste (Sigma). This was treated to form a colloidal suspension of chitin or chitosan crystallites. The electrostatic 'charge coat' surrounding the chitin was then manipulated. This was achieved by alteration of the pH of the chitin or chitosan ...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/s0141-8130(98)00002-6

    authors： Murray SB,Neville AC

    更新日期：1998-04-01 00:00:00

  • Preparation and evaluation of Bletilla striata polysaccharide/graphene oxide composite hemostatic sponge.

    abstract：:Uncontrolled bleeding is an important cause of military and civilian casualties. GO has received more attention in the field of hemostasis. However, pure GO has various limitation in application due to its potential thrombosis, hemolytic and cytotoxicity. Herein, we present a simple, rapid and low-cost method to combi...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2019.02.137

    authors： Chen J,Lv L,Li Y,Ren X,Luo H,Gao Y,Yan H,Li Y,Qu Y,Yang L,Li X,Zeng R

    更新日期：2019-06-01 00:00:00

  • DNA binding and nuclease activity of an oxovanadium valinato-Schiff base complex.

    abstract：:A new oxovanadium complex [VO(sal-l-val)(phen)] (sal-l-val=Schiff base derived from salicylaldehyde and l-valine; phen=1,10-phenanthroline) has been designed and synthesized with the aim of developing potential DNA nuclease. The interaction of DNA with this structurally characterized oxovanadium complex has been studi...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2014.02.033

    authors： Saha U,Mukherjea KK

    更新日期：2014-05-01 00:00:00

  • Effect of plasticizers on microstructure, compatibility and mechanical property of hydroxypropyl methylcellulose/hydroxypropyl starch blends.

    abstract：:Polymer film blends of hydroxypropyl methylcellulose (HPMC) and hydroxypropyl starch (HPS) were produced with an incorporation of three different plasticizers, including polyethylene glycol (PEG), glycerol and 1, 2-propylene glycol (PG) respectively, and the influence of these plasticizers were compared and studied by...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2018.07.064

    authors： Zhang L,Wang XF,Liu H,Yu L,Wang Y,Simon GP,Qian J

    更新日期：2018-11-01 00:00:00

  • Effect of chitosan molecular weight on zein-chitosan nanocomplexes: Formation, characterization, and the delivery of quercetagetin.

    abstract：:The impacts of chitosan with different molecular weight on the formation mechanism, structural characteristics and encapsulating properties of the zein-chitosan nanocomplexes were investigated. The results of dynamic light scattering showed that the mean size of nanocomplexes was increased and became more highly posit...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2020.07.245

    authors： Chen S,Ma X,Han Y,Wei Y,Guo Q,Yang S,Zhang Y,Liao W,Gao Y

    更新日期：2020-12-01 00:00:00

  • Collapse of DNA in packaging and cellular transport.

    abstract：:The dawn of molecular biology and recombinant DNA technology arose from our ability to manipulate DNA, including the process of collapse of long extended DNA molecules into nanoparticles of approximately 100 nm diameter. This condensation process is important for the packaging of DNA in the cell and for transporting D...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章,评审

    doi：10.1016/j.ijbiomac.2017.12.076

    authors： Thomas TJ,Thomas T

    更新日期：2018-04-01 00:00:00

  • Role of 13-(di)phenylalkyl berberine derivatives in the modulation of the activity of human topoisomerase IB.

    abstract：:Topoisomerases IB are anticancer and antimicrobial targets whose inhibition by several natural and non-natural compounds has been documented. The inhibition effect by berberine and some 13-(di)phenylalkyl berberine derivatives has been tested towards human topoisomerase IB. Derivatives belonging to the 13-diphenylalky...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2015.02.051

    authors： Vieira S,Castelli S,Falconi M,Takarada J,Fiorillo G,Buzzetti F,Lombardi P,Desideri A

    更新日期：2015-01-01 00:00:00

  • Characterization of a new endo-type alginate lyase from Vibrio sp. W13.

    abstract：:A gene, encoding a new alginate lyase Algb, was identified and cloned from marine bacterium Vibrio sp. W13. The recombinant alginate lyase was characterized followed by being purified on Ni-NTA Sepharose. It exhibited the highest activity (457 U/mg) at pH 8.0 and 30 °C. Interestingly, Algb possessed broader substrate ...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2015.01.053

    authors： Zhu B,Tan H,Qin Y,Xu Q,Du Y,Yin H

    更新日期：2015-04-01 00:00:00

  • Evaluation of polyurethane based on cellulose derivative-ketoprofen biosystem for implant biomedical devices.

    abstract：:A polyether-urethane based on polytetrahydrofuran containing hydroxypropyl cellulose for biomedical applications was tested for its biocompatibility. Ketoprofen was incorporated (3% and 6%) in the polyurethane matrix as an anti-inflammatory drug. Dynamic vapour sorption method was employed for testing the water sorpti...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2012.09.026

    authors： Macocinschi D,Filip D,Vlad S,Butnaru M,Knieling L

    更新日期：2013-01-01 00:00:00

  • A polysaccharide from Hedyotis diffusa interrupts metastatic potential of lung adenocarcinoma A549 cells by inhibiting EMT via EGFR/Akt/ERK signaling pathways.

    abstract：:In this study we investigated the potential effects of a polysaccharide (HDP) from Hedyotis diffusa on the metastasis in human lung adenocarcinoma A549 cells. HDP (25, 50 and 100μg/ml) significantly suppressed the cell adhesion, invasion and migration of A549 cells in a dose dependent manner by downregulation of matri...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2019.02.040

    authors： Lin L,Cheng K,He Z,Lin Q,Huang Y,Chen C,Xie Z,Chen L,Liang Z

    更新日期：2019-05-15 00:00:00

  • A cyclic disulfide peptide reproduces in solution the main structural features of a native antigenic site of foot-and-mouth disease virus.

    abstract：:A cyclic disulfide peptide corresponding to the G-H loop sequence 134-155 [replacement Tyr136 and Arg153 with Cys] of the capsid protein VP1 of foot-and-mouth disease virus (FMDV) isolate C-S8c1 was examined by proton 2D-NMR spectroscopy in water and in 25% HFIP/water. In water, NMR data supported the presence of a no...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/s0141-8130(97)01163-x

    authors： Haack T,Camarero JA,Roig X,Mateu MG,Domingo E,Andreu D,Giralt E

    更新日期：1997-06-01 00:00:00

  • Partial purification and MALDI-TOF MS analysis of UN1, a tumor antigen membrane glycoprotein.

    abstract：:UN1 is a membrane glycoprotein that is expressed in immature human thymocytes, a subpopulation of peripheral T lymphocytes, the HPB acute lymphoblastic leukemia (ALL) T-cell line and fetal thymus. We previously reported the isolation of a monoclonal antibody (UN1 mAb) recognizing the UN1 protein that was classified as...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2006.02.020

    authors： de Laurentiis A,Caterino M,Orrù S,Ruoppolo M,Tuccillo F,Masullo M,Quinto I,Scala G,Pucci P,Palmieri C,Tassone P,Salvatore F,Venuta S

    更新日期：2006-08-15 00:00:00