当前位置： SCI文献检索 > Scientific Reports期刊下所有文献 > Single molecule super-resolution imaging of bacterial cell pole proteins with high-throughput quantitative analysis pipeline.

Single molecule super-resolution imaging of bacterial cell pole proteins with high-throughput quantitative analysis pipeline.

Abstract：

:Bacteria show sophisticated control of their cellular organization, and many bacteria deploy different polar landmark proteins to organize the cell pole. Super-resolution microscopy, such as Photo-Activated Localization Microscopy (PALM), provides the nanoscale localization of molecules and is crucial for better understanding of organization and dynamics in single-molecule. However, analytical tools are not fully available yet, in particular for bacterial cell biology. For example, quantitative and statistical analyses of subcellular localization with multiple cells from multiple fields of view are lacking. Furthermore, brightfield images are not sufficient to get accurate contours of small and low contrast bacterial cells, compared to subpixel presentation of target molecules. Here we describe a novel analytic tool for PALM which integrates precisely drawn cell outlines, of either inner membrane or periplasm, labelled by PALM-compatible fluorescent protein fusions, with molecule data for >10,000 molecules from >100 cells by fitting each cell into an oval arc. In the vibrioid bacterium Vibrio cholerae, the polar anchor HubP constitutes a big polar complex which includes multiple proteins involved in chemotaxis and the flagellum. With this pipeline, HubP is shown to be slightly skewed towards the inner curvature side of the cell, while its interaction partners showed rather loose polar localization.

journal_name

Sci Rep

journal_title

Scientific reports

authors

Altinoglu I,Merrifield CJ,Yamaichi Y

doi

10.1038/s41598-019-43051-7

subject

Has Abstract

pub_date

2019-04-30 00:00:00

pages

6680

issue

1

issn

2045-2322

pii

10.1038/s41598-019-43051-7

journal_volume

9

pub_type

杂志文章

相关文献

Scientific Reports文献大全
  • mRNA Transfection-Induced Activation of Primary Human Monocytes and Macrophages: Dependence on Carrier System and Nucleotide Modification.

    abstract：:Monocytes and macrophages are key players in maintaining immune homeostasis. Identifying strategies to manipulate their functions via gene delivery is thus of great interest for immunological research and biomedical applications. We set out to establish conditions for mRNA transfection in hard-to-transfect primary hum...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-020-60506-4

    authors： Moradian H,Roch T,Lendlein A,Gossen M

    更新日期：2020-03-06 00:00:00

  • Publisher Correction: Re-estimation improved the performance of two Framingham cardiovascular risk equations and the Pooled Cohort equations: A nationwide registry analysis.

    abstract：:An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...

    journal_title：Scientific reports

    pub_type： 已发布勘误

    doi：10.1038/s41598-020-67920-8

    authors： Wallisch C,Heinze G,Rinner C,Mundigler G,Winkelmayer WC,Dunkler D

    更新日期：2020-06-26 00:00:00

  • Insecticide resistance in the bed bug comes with a cost.

    abstract：:Adaptation to new environmental stress is often associated with an alteration of one or more life history parameters. Insecticide resistant populations of insects often have reduced fitness relative to susceptible populations in insecticide free environments. Our previous work showed that three populations of bed bugs...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep10807

    authors： Gordon JR,Potter MF,Haynes KF

    更新日期：2015-06-03 00:00:00

  • Solar-mediated thermo-electrochemical oxidation of sodium dodecyl benzene sulfonate by modulating the effective oxidation potential and pathway for green remediation of wastewater.

    abstract：:To match the relentless pursuit of three research hot points - efficient solar utilization, green and sustainable remediation of wastewater and advanced oxidation processes, solar-mediated thermo-electrochemical oxidation of surfactant was proposed and developed for green remediation of surfactant wastewater. The sola...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep44683

    authors： Gu D,Gao S,Jiang T,Wang B

    更新日期：2017-03-15 00:00:00

  • Mechanical perturbations trigger endothelial nitric oxide synthase activity in human red blood cells.

    abstract：:Nitric oxide (NO), a vascular signaling molecule, is primarily produced by endothelial NO synthase. Recently, a functional endothelial NO synthase (eNOS) was described in red blood cells (RBC). The RBC-eNOS contributes to the intravascular NO pool and regulates physiological functions. However the regulatory mechanism...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep26935

    authors： Nagarajan S,Raj RK,Saravanakumar V,Balaguru UM,Behera J,Rajendran VK,Shathya Y,Ali BM,Sumantran V,Chatterjee S

    更新日期：2016-06-27 00:00:00

  • Recombinant IFN-γ from the bank vole Myodes glareolus: a novel tool for research on rodent reservoirs of zoonotic pathogens.

    abstract：:Rodent species like Myodes glareolus and Microtus spp. are natural reservoirs for many zoonotic pathogens causing human diseases and are gaining increasing interest in the field of eco-immunology as candidate animal models. Despite their importance the lack of immunological reagents has hampered research in these anim...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-018-21143-0

    authors： Torelli F,Zander S,Ellerbrok H,Kochs G,Ulrich RG,Klotz C,Seeber F

    更新日期：2018-02-12 00:00:00

  • Polymorphisms in the P2X7 receptor, and differential expression of Toll-like receptor-mediated cytokines and defensins, in a Canadian Indigenous group.

    abstract：:Canadian Indigenous peoples (First Nations and Inuit) exhibit a high burden of infectious diseases including tuberculosis influenced by societal factors, and biological determinants. Toll-like receptor (TLR)-mediated innate immune responses are the first line of defence against infections. We examined the production o...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-019-50596-0

    authors： Semple C,Choi KG,Kroeker A,Denechezhe L,Orr P,Mookherjee N,Larcombe L

    更新日期：2019-10-02 00:00:00

  • Elucidating the Performance Limitations of Lithium-ion Batteries due to Species and Charge Transport through Five Characteristic Parameters.

    abstract：:Underutilization due to performance limitations imposed by species and charge transports is one of the key issues that persist with various lithium-ion batteries. To elucidate the relevant mechanisms, two groups of characteristic parameters were proposed. The first group contains three characteristic time parameters, ...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep32639

    authors： Jiang F,Peng P

    更新日期：2016-09-07 00:00:00

  • A dynamic prediction model for prognosis of acute-on-chronic liver failure based on the trend of clinical indicators.

    abstract：:Acute-on-chronic liver failure (ACLF) is a dynamic syndrome, and sequential assessments can reflect its prognosis more accurately. Our aim was to build and validate a new scoring system to predict short-term prognosis using baseline and dynamic data in ACLF. We conducted a retrospective cohort analysis of patients wit...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-021-81431-0

    authors： Yu Z,Zhang Y,Cao Y,Xu M,You S,Chen Y,Zhu B,Kong M,Song F,Xin S,Duan Z,Han T

    更新日期：2021-01-19 00:00:00

  • Increase in α-tubulin modifications in the neuronal processes of hippocampal neurons in both kainic acid-induced epileptic seizure and Alzheimer's disease.

    abstract：:Neurodegeneration includes acute changes and slow-developing alterations, both of which partly involve common cellular machinery. During neurodegeneration, neuronal processes are impaired along with dysregulated post-translational modifications (PTMs) of cytoskeletal proteins. In neuronal processes, tubulin undergoes ...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep40205

    authors： Vu HT,Akatsu H,Hashizume Y,Setou M,Ikegami K

    更新日期：2017-01-09 00:00:00

  • Association between the ambient temperature and the occurrence of human Salmonella and Campylobacter infections.

    abstract：:Salmonella spp. and thermotolerant Campylobacter spp. are the most important causes of human bacterial diarrheal infections worldwide. These bacterial species are influenced by several factors like behaviour of the host, shedding, environment incl. directly or indirectly through ambient temperature, and the infections...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep28442

    authors： Yun J,Greiner M,Höller C,Messelhäusser U,Rampp A,Klein G

    更新日期：2016-06-21 00:00:00

  • Author Correction: A Systematic Evaluation of Hospital Performance of Childbirth Delivery Modes and Associated Factors in the Friuli Venezia Giulia Region (North-Eastern Italy), 2005-2015.

    abstract：:An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...

    journal_title：Scientific reports

    pub_type： 已发布勘误

    doi：10.1038/s41598-020-62742-0

    authors： Cegolon L,Mastrangelo G,Heymann WC,Dal Pozzo G,Ronfani L,Barbone F

    更新日期：2020-04-10 00:00:00

  • A novel technique based on in vitro oocyte injection to improve CRISPR/Cas9 gene editing in zebrafish.

    abstract：:Contemporary improvements in the type II clustered regularly interspaced short palindromic repeats/CRISPR-associated protein 9 (CRISPR/Cas9) system offer a convenient way for genome editing in zebrafish. However, the low efficiencies of genome editing and germline transmission require a time-intensive and laborious sc...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep34555

    authors： Xie SL,Bian WP,Wang C,Junaid M,Zou JX,Pei DS

    更新日期：2016-09-29 00:00:00

  • Association of plasma C-reactive protein level with the prevalence of colorectal adenoma: the Colorectal Adenoma Study in Tokyo.

    abstract：:Epidemiologic studies have identified a positive association between obesity and colorectal neoplasia. Adiposity induces systemic low-grade inflammation, which is commonly assessed with a sensitive biomarker, C-reactive protein (CRP). To understand the molecular mechanisms of obesity in the etiology of colorectal neop...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-017-04780-9

    authors： Kigawa N,Budhathoki S,Yamaji T,Iwasaki M,Inoue M,Tsugane S

    更新日期：2017-06-30 00:00:00

  • Defining eligible patients for allele-selective chemotherapies targeting NAT2 in colorectal cancer.

    abstract：:Therapies targeting somatic bystander genetic events represent a new avenue for cancer treatment. We recently identified a subset of colorectal cancer (CRC) patients who are heterozygous for a wild-type and a low activity allele (NAT2*6) but lack the wild-type allele in their tumors due to loss of heterozygosity (LOH)...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-020-80288-z

    authors： Rendo V,Kundu S,Rameika N,Ljungström V,Svensson R,Palin K,Aaltonen L,Stoimenov I,Sjöblom T

    更新日期：2020-12-31 00:00:00

  • Coarsening behaviour of M23C6 carbides in creep-resistant steel exposed to high temperatures.

    abstract：:High operating temperatures can have very deleterious effects on the long-term performance of high-Cr, creep-resistant steels used, for example, in the structural components of power plants. For the popular creep-resistant steel X20CrMoV12.1 we analysed the processes of carbide growth using a variety of analytical tec...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep29734

    authors： Godec M,Skobir Balantič DA

    更新日期：2016-07-13 00:00:00

  • Discovery of a new Theileria sp. closely related to Theileria annulata in cattle from Sri Lanka.

    abstract：:Theileria annulata is a haemoprotozoan parasite that causes a cancer-like illness known as tropical theileriosis in cattle. In the course of analyzing the genetic diversity of T. annulata in Sri Lanka, we observed that merozoite-piroplasm surface antigen (tams1) and surface protein (tasp)-like gene sequences obtained ...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-019-52512-y

    authors： Sivakumar T,Fujita S,Tuvshintulga B,Kothalawala H,Silva SSP,Yokoyama N

    更新日期：2019-11-06 00:00:00

  • PDGFRA gene, maternal binge drinking and obstructive heart defects.

    abstract：:Obstructive heart defects (OHDs) are a major health concern worldwide. The platelet-derived growth factor (PDGF) genes are known to have regulatory functions that are essential for proper heart development. In a zebrafish model, Pdgfra was further demonstrated to interact with ethanol during craniofacial development. ...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-018-29160-9

    authors： Tang X,Eberhart JK,Cleves MA,Li J,Li M,MacLeod S,Nembhard WN,Hobbs CA

    更新日期：2018-07-23 00:00:00

  • Incorporation and antimicrobial activity of nisin Z within carrageenan/chitosan multilayers.

    abstract：:An antimicrobial peptide, nisin Z, was embedded within polyelectrolyte multilayers (PEMs) composed of natural polysaccharides in order to explore the potential of forming a multilayer with antimicrobial properties. Using attenuated total reflection Fourier transform infrared spectroscopy (ATR FTIR), the formation of c...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-020-79702-3

    authors： Webber JL,Namivandi-Zangeneh R,Drozdek S,Wilk KA,Boyer C,Wong EHH,Bradshaw-Hajek BH,Krasowska M,Beattie DA

    更新日期：2021-01-18 00:00:00

  • Enhanced near-surface ozone under heatwave conditions in a Mediterranean island.

    abstract：:Near-surface ozone is enhanced under particular chemical reactions and physical processes. This study showed the seasonal variation of near-surface ozone in Nicosia, Cyprus and focused in summers when the highest ozone levels were noted using a seven year hourly dataset from 2007 to 2014. The originality of this study...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-018-27590-z

    authors： Pyrgou A,Hadjinicolaou P,Santamouris M

    更新日期：2018-06-15 00:00:00

  • Multivariate simulation framework reveals performance of multi-trait GWAS methods.

    abstract：:Burgeoning availability of genome-wide association study (GWAS) results and national biobank data has led to growing interest in performing multi-trait genetic analyses. Numerous multi-trait GWAS methods that exploit either summary statistics or individual-level data have been developed, but their relative performance...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep38837

    authors： Porter HF,O'Reilly PF

    更新日期：2017-03-13 00:00:00

  • Tumor resection ameliorates tumor-induced suppression of neuroinflammatory and behavioral responses to an immune challenge in a cancer survivor model.

    abstract：:Breast cancer survivors display altered inflammatory responses to immune challenges relative to cancer-naive controls likely due to previous cancer treatments, stress associated with cancer, and/or tumor physiology. Proper inflammatory responses are necessary for adaptive sickness behaviors (e.g., fatigue, anorexia, a...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-018-37334-8

    authors： Santos JC,Bever SR,Pereira-da-Silva G,Pyter LM

    更新日期：2019-01-24 00:00:00

  • Shortened leukocyte telomere length is associated with reduced pulmonary function and greater subsequent decline in function in a sample of World Trade Center responders.

    abstract：:The objective of this study was to examine whether shorter leukocyte telomere length (LTL) is associated with more rapid pulmonary function decline in a longitudinal study of World Trade Center (WTC) responders. WTC responders (N = 284) participating in a monitoring study underwent blood sampling and were followed pro...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-019-44625-1

    authors： Clouston SAP,Edelman NH,Aviv A,Stewart C,Luft BJ

    更新日期：2019-05-31 00:00:00

  • Publisher Correction: Mass & secondary structure propensity of amino acids explain their mutability and evolutionary replacements.

    abstract：:A correction to this article has been published and is linked from the HTML and PDF versions of this paper. The error has been fixed in the paper. ...

    journal_title：Scientific reports

    pub_type： 已发布勘误

    doi：10.1038/s41598-018-21981-y

    authors： Bohórquez HJ,Suárez CF,Patarroyo ME

    更新日期：2018-03-06 00:00:00

  • Mechanical forces induce an asthma gene signature in healthy airway epithelial cells.

    abstract：:Bronchospasm compresses the bronchial epithelium, and this compressive stress has been implicated in asthma pathogenesis. However, the molecular mechanisms by which this compressive stress alters pathways relevant to disease are not well understood. Using air-liquid interface cultures of primary human bronchial epithe...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-020-57755-8

    authors： Kılıç A,Ameli A,Park JA,Kho AT,Tantisira K,Santolini M,Cheng F,Mitchel JA,McGill M,O'Sullivan MJ,De Marzio M,Sharma A,Randell SH,Drazen JM,Fredberg JJ,Weiss ST

    更新日期：2020-01-22 00:00:00

  • Patho- physiological role of BDNF in fibrin clotting.

    abstract：:Circulating levels of Brain Derived Neurotrophic Factor (BDNF) are lower in coronary heart disease (CHD) than in healthy subjects and are associated with coronary events and mortality. However, the mechanism(s) underling this association is not fully understood. We hypothesize that BDNF may influence fibrin fiber stru...

    journal_title：Scientific reports

    pub_type： 临床试验,杂志文章

    doi：10.1038/s41598-018-37117-1

    authors： Amadio P,Porro B,Sandrini L,Fiorelli S,Bonomi A,Cavalca V,Brambilla M,Camera M,Veglia F,Tremoli E,Barbieri SS

    更新日期：2019-01-23 00:00:00

  • Polymorphisms in Protamine 1 and Protamine 2 predict the risk of male infertility: a meta-analysis.

    abstract：:Several studies have investigated the association between polymorphisms in protamine 1 and 2 genes and male infertility risk, with inconsistent results to date. This meta-analysis based on the 13 published case-control studies, including 7350 cases and 6167 controls, was performed to further establish the potential as...

    journal_title：Scientific reports

    pub_type： 杂志文章,meta分析

    doi：10.1038/srep15300

    authors： Jiang W,Sun H,Zhang J,Zhou Q,Wu Q,Li T,Zhang C,Li W,Zhang M,Xia X

    更新日期：2015-10-16 00:00:00

  • High yield of protein-rich forage rice achieved by soil amendment with composted sewage sludge and topdressing with treated wastewater.

    abstract：:Aiming to promote low-cost production of protein-rich forage rice and resource recycling from wastewater treatment plants, a pot experiment was conducted to assess the possibility to substitute mineral fertilizers with composted sewage sludge (CSS) with/without top-dressing with treated municipal wastewater (TWW). Res...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-020-67233-w

    authors： Phung LD,Ichikawa M,Pham DV,Sasaki A,Watanabe T

    更新日期：2020-06-23 00:00:00

  • Quantum-enhanced absorption refrigerators.

    abstract：:Thermodynamics is a branch of science blessed by an unparalleled combination of generality of scope and formal simplicity. Based on few natural assumptions together with the four laws, it sets the boundaries between possible and impossible in macroscopic aggregates of matter. This triggered groundbreaking achievements...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep03949

    authors： Correa LA,Palao JP,Alonso D,Adesso G

    更新日期：2014-02-04 00:00:00

  • The neural transfer effect of working memory training to enhance hedonic processing in individuals with social anhedonia.

    abstract：:Anhedonia, the diminished ability to experience pleasure, is a challenging negative symptom in patients with schizophrenia and can be observed in at-risk individuals with schizotypy. Deficits in hedonic processing have been postulated to be related to decreased motivation to engage in potentially rewarding events. It ...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep35481

    authors： Li X,Li Z,Li K,Zeng YW,Shi HS,Xie WL,Yang ZY,Lui SS,Cheung EF,Leung AW,Chan RC

    更新日期：2016-10-18 00:00:00