Abstract：
:Nitric oxide (NO), a vascular signaling molecule, is primarily produced by endothelial NO synthase. Recently, a functional endothelial NO synthase (eNOS) was described in red blood cells (RBC). The RBC-eNOS contributes to the intravascular NO pool and regulates physiological functions. However the regulatory mechanisms and clinical implications of RBC-eNOS are unknown. The present study investigated regulation and functions of RBC-eNOS under mechanical stimulation. This study shows that mechanical stimuli perturb RBC membrane, which triggers a signaling cascade to activate the eNOS. Extracellular NO level, estimated by the 4-Amino-5-Methylamino-2', 7'-Difluorofluorescein Diacetate probe, was significantly increased under mechanical stimuli. Immunostaining and western blot studies confirmed that the mechanical stimuli phosphorylate the serine 1177 moiety of RBC-eNOS, and activates the enzyme. The NO produced by activation of RBC-eNOS in vortexed RBCs promoted important endothelial functions such as migration and vascular sprouting. We also show that mechanical perturbation facilitates nitrosylation of RBC proteins via eNOS activation. The results of the study confirm that mechanical perturbations sensitize RBC-eNOS to produce NO, which ultimately defines physiological boundaries of RBC structure and functions. Therefore, we propose that mild physical perturbations before, after, or during storage can improve viability of RBCs in blood banks.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Nagarajan S,Raj RK,Saravanakumar V,Balaguru UM,Behera J,Rajendran VK,Shathya Y,Ali BM,Sumantran V,Chatterjee Sdoi
10.1038/srep26935subject
Has Abstractpub_date
2016-06-27 00:00:00pages
26935issn
2045-2322pii
srep26935journal_volume
6pub_type
杂志文章abstract：:The remineralization mechanism in dental caries lesions is not completely understood. This study reports on ultrastructural and chemical changes observed within arrested caries lesions. Carious human teeth were observed using scanning electron microscopy (SEM) and focused-ion-beam (FIB)-SEM. The crystals detected in t...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-61111-1
更新日期：2020-03-09 00:00:00
abstract：:Mouse models have contributed to the bulk of knowledge on Systemic Lupus Erythematosus (SLE). Nevertheless, substantial differences exist between human and mouse immune system. We aimed to establish and characterise a SLE model mediated by human immune system. Injection of pristane into immunodeficient mice reconstitu...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-16999-7
更新日期：2017-11-30 00:00:00
abstract：:Meishan boars are known for their early sexual maturity. However, they exhibit a significantly smaller testicular size and a reduced proportion of Sertoli cells and daily sperm production compared with Duroc boars. The testes of Duroc and Meishan boars at 20, 75 and 270 days of age were used for histological and trans...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep20758
更新日期：2016-02-11 00:00:00
abstract：:Zebrafish embryos are translucent and develop rapidly in individual eggs ex utero; they are widely used as models for embryogenesis and organ development for human diseases and drug discovery. Lysine crotonylation (Kcr) is a type of histone post-translational modifications discovered in 2011. Kcr dynamics are involved...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-22069-3
更新日期：2018-02-26 00:00:00
abstract：:Although large populations feel fatigue, the standardized medicinal therapy is currently absent. In this study, we determined whether 5-aminolevulinic acid (5-ALA) supplementation alleviates the feeling of fatigue in healthy subjects who feel chronic physical tiredness. Males and females between ages of 20 and 64 who ...
journal_title：Scientific reports
pub_type： 杂志文章,随机对照试验
doi：10.1038/s41598-020-72763-4
更新日期：2020-09-29 00:00:00
abstract：:A correction to this article has been published and is linked from the HTML and PDF versions of this paper. The error has been fixed in the paper. ...
journal_title：Scientific reports
pub_type： 已发布勘误
doi：10.1038/s41598-018-31524-0
更新日期：2018-09-03 00:00:00
abstract：:As an important method for building blocks synthesis, whole cell biocatalysis is hindered by some shortcomings such as unpredictability of reactions, utilization of opportunistic pathogen, and side reactions. Due to its biological and extensively studied genetic background, Pseudomonas putida KT2440 is viewed as a pro...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep06939
更新日期：2014-11-06 00:00:00
abstract：:A considerable body of evidence links together mitochondrial dysfunctions, toxic action of metalloid oxyanions, and system and neurodegenerative disorders. In this study we have used the model yeast Saccharomyces cerevisiae to investigate the genetic determinants associated with tellurite resistance/sensitivity. Nitro...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-30479-6
更新日期：2018-08-13 00:00:00
abstract：:While much of memory research takes an observer-centric focus looking at participant performance, recent work has pinpointed important item-centric effects on memory, or how intrinsically memorable a given stimulus is. However, little is known about the neural correlates of memorability during memory retrieval, or how...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-26467-5
更新日期：2018-06-06 00:00:00
abstract：:During value-based decision making, ventromedial prefrontal cortex (vmPFC) is thought to support choices by tracking the expected gain from different outcomes via a competition-based process. Using a computational neurostimulation approach we asked how perturbing this region might alter this competition and resulting ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep25160
更新日期：2016-05-05 00:00:00
abstract：:Whole-genome sequencing is likely to become increasingly used by local clinical microbiology laboratories, where sequencing volume is low compared with national reference laboratories. Here, we describe a universal protocol for simultaneous DNA extraction and sequencing of numerous different bacterial species, allowin...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-80031-8
更新日期：2021-01-08 00:00:00
abstract：:The controversial anti-proliferative effects of Echinacea purpurea (L.) Moench (Asteraceae) might be related to different plant metabolites contained in plant samples, extracts and products. The influence of bacterial endophytes on the synthesis of bioactive compounds in the medicinal plants has been previously demons...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-47329-8
更新日期：2019-07-26 00:00:00
abstract：:Antimicrobial peptides (AMPs) exhibit broad-spectrum antimicrobial activity, and have promise as new therapeutic agents. While the adult North American bullfrog (Rana [Lithobates] catesbeiana) is a prolific source of high-potency AMPs, the aquatic tadpole represents a relatively untapped source for new AMP discovery. ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-38442-1
更新日期：2019-02-06 00:00:00
abstract：:Women who have previously had an induced abortion (IA) before their first birth have been associated with preterm birth (PTB). However, previous studies on the PTB are inconsistent. Therefore, the aim of this study was to clarify the association between IA and PTB and low birth weight (LBW) for first-time mothers. A t...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-23695-7
更新日期：2018-03-29 00:00:00
abstract：:The incidence of cancer in the United Kingdom has increased significantly over the last four decades. The aim of this study was to examine trends in UK cancer incidence and mortality by cancer site and assess the potential for overdiagnosis. Using Cancer Research UK incidence and mortality data for the period (1971-20...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-32844-x
更新日期：2018-10-02 00:00:00
abstract：:Spinal Muscular Atrophy (SMA) is caused by mutation or deletion of the survival motor neuron 1 (SMN1) gene. Decreased levels of, cell-ubiquitous, SMN protein is associated with a range of systemic pathologies reported in severe patients. Despite high levels of SMN protein in normal liver, there is no comprehensive stu...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep34635
更新日期：2016-10-04 00:00:00
abstract：:Cardiac remodeling is characterized by overactivity of the renin-angiotensin system (RAS) and withdrawal of vagal activity. We hypothesized that improving vagal activity could attenuate cardiac fibrosis induced by angiotensin II (Ang II) in vivo and in vitro. Rats were subjected to abdominal aorta constriction (AAC) w...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep17108
更新日期：2015-11-24 00:00:00
abstract：:The Ah receptor (AhR) is a ligand-dependent transcription factor belonging to the basic helix-loop-helix Per-Arnt-Sim (bHLH-PAS) superfamily. Binding to and activation of the AhR by a variety of chemicals results in the induction of expression of diverse genes and production of a broad spectrum of biological and toxic...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-47138-z
更新日期：2019-07-23 00:00:00
abstract：:Metastatic colorectal cancer is the fourth most common cause of cancer death. Current options in palliation such as hyperthermic intraperitoneal chemotherapy (HIPEC) present severe side effects. Recent research efforts suggested the therapeutic use of oxidant-enriched liquid using cold physical plasma. To investigate ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-37169-3
更新日期：2019-01-24 00:00:00
abstract：:Tissue microarray (TMA) core images are a treasure trove for artificial intelligence applications. However, a common problem of TMAs is multiple sectioning, which can change the content of the intended tissue core and requires re-labelling. Here, we investigate different ensemble methods for colorectal tissue classifi...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-021-81352-y
更新日期：2021-01-27 00:00:00
abstract：:Chitin is a valuable peat substrate amendment by increasing lettuce growth and reducing the survival of the zoonotic pathogen Salmonella enterica on lettuce leaves. The production of chitin-catabolic enzymes (chitinases) play a crucial role and are mediated through the microbial community. A higher abundance of plant-...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-46106-x
更新日期：2019-07-09 00:00:00
abstract：:There has been a developing technology in algae with pharmaceuticals wastewater. However, the effect and the underlying mechanism of pharmaceuticals on algae are not well understood. To investigate the effect and mechanism of pharmaceuticalson microalgae, four pharmaceuticals of clofibric acid (CLF), ciprofloxacin (CI...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-36609-4
更新日期：2019-02-07 00:00:00
abstract：:Proton pump inhibitors (PPIs) belong to the most common medication in geriatric medicine. They are known to reduce osteoclast activity and to delay fracture healing in young adult mice. Because differentiation and proliferation in fracture healing as well as pharmacologic actions of drugs markedly differ in the elderl...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-79605-3
更新日期：2020-12-23 00:00:00
abstract：:Typhoid fever poses significant burden on healthcare systems in Southeast Asia and other endemic countries. Several epidemiological and genomic studies have attributed pseudogenisation to be the major driving force for the evolution of Salmonella Typhi although its real potential remains elusive. In the present study,...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep07457
更新日期：2014-12-12 00:00:00
abstract：:Pulmonary arterial hypertension is a progressive disease that causes the obstruction of precapillary pulmonary arteries and a sustained increase in pulmonary vascular resistance. The aim was to analyze functionally the variants found in the BMPR2 gene and to establish a genotype-phenotype correlation. mRNA expression ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-02074-8
更新日期：2017-05-15 00:00:00
abstract：:Waves of any kinds, including sound waves and light waves, can interfere constructively or destructively when they are overlapped, allowing for myriad applications. However, unlike continuous waves of a single frequency, interference of photoacoustic pulses is often overlooked because of their broadband characteristic...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep21511
更新日期：2016-02-12 00:00:00
abstract：:C. canimorsus and C. cynodegmi are dog and cat commensals which can be transmitted to humans via bites or scratches and can cause sepsis, meningitis, endocarditis, and eye- or wound infections. Recently an additional Capnocytophaga species was identified as part of the oral flora of healthy dogs and was given the name...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep22919
更新日期：2016-03-07 00:00:00
abstract：:The toxicity associated with concentrated synthetic surfactants and the poor stability at gastrointestinal condition are two major constraints for practical applications of solid lipid nanoparticles (SLN) as oral delivery vehicles. In this study, a synthetic surfactant-free and cross-linker-free method was developed t...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-02867-x
更新日期：2017-06-05 00:00:00
abstract：:The eastern North American migratory population of monarch butterflies (Danaus plexippus) is in serious decline. Habitat restoration, including adding millions of host plants to compensate for loss of milkweed in US cropland, is a key part of the international conservation strategy to return this iconic butterfly to s...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-30731-z
更新日期：2018-08-14 00:00:00
abstract：:Inhomogeneity in the ground state is an intriguing, emergent phenomenon in magnetism. Recently, it has been observed in the magnetostructural channel of the geometrically frustrated α-NaMnO2, for the first time in the absence of active charge degrees of freedom. Here we report an in-depth numerical and local-probe exp...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep09272
更新日期：2015-03-19 00:00:00
