Incorporation and antimicrobial activity of nisin Z within carrageenan/chitosan multilayers.

Abstract:

:An antimicrobial peptide, nisin Z, was embedded within polyelectrolyte multilayers (PEMs) composed of natural polysaccharides in order to explore the potential of forming a multilayer with antimicrobial properties. Using attenuated total reflection Fourier transform infrared spectroscopy (ATR FTIR), the formation of carrageenan/chitosan multilayers and the inclusion of nisin Z in two different configurations was investigated. Approximately 0.89 µg cm-2 nisin Z was contained within a 4.5 bilayer film. The antimicrobial properties of these films were also investigated. The peptide containing films were able to kill over 90% and 99% of planktonic and biofilm cells, respectively, against Staphylococcus aureus and methicillin-resistant Staphylococcus aureus (MRSA) strains compared to control films. Additionally, surface topography and wettability studies using atomic force microscopy (AFM) and the captive bubble technique revealed that surface roughness and hydrophobicity was similar for both nisin containing multilayers. This suggests that the antimicrobial efficacy of the peptide is unaffected by its location within the multilayer. Overall, these results demonstrate the potential to embed and protect natural antimicrobials within a multilayer to create functionalised coatings that may be desired by industry, such as in the food, biomaterials, and pharmaceutical industry sectors.

journal_name

Sci Rep

journal_title

Scientific reports

authors

Webber JL,Namivandi-Zangeneh R,Drozdek S,Wilk KA,Boyer C,Wong EHH,Bradshaw-Hajek BH,Krasowska M,Beattie DA

doi

10.1038/s41598-020-79702-3

subject

Has Abstract

pub_date

2021-01-18 00:00:00

pages

1690

issue

1

issn

2045-2322

pii

10.1038/s41598-020-79702-3

journal_volume

11

pub_type

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