Abstract:
:An antimicrobial peptide, nisin Z, was embedded within polyelectrolyte multilayers (PEMs) composed of natural polysaccharides in order to explore the potential of forming a multilayer with antimicrobial properties. Using attenuated total reflection Fourier transform infrared spectroscopy (ATR FTIR), the formation of carrageenan/chitosan multilayers and the inclusion of nisin Z in two different configurations was investigated. Approximately 0.89 µg cm-2 nisin Z was contained within a 4.5 bilayer film. The antimicrobial properties of these films were also investigated. The peptide containing films were able to kill over 90% and 99% of planktonic and biofilm cells, respectively, against Staphylococcus aureus and methicillin-resistant Staphylococcus aureus (MRSA) strains compared to control films. Additionally, surface topography and wettability studies using atomic force microscopy (AFM) and the captive bubble technique revealed that surface roughness and hydrophobicity was similar for both nisin containing multilayers. This suggests that the antimicrobial efficacy of the peptide is unaffected by its location within the multilayer. Overall, these results demonstrate the potential to embed and protect natural antimicrobials within a multilayer to create functionalised coatings that may be desired by industry, such as in the food, biomaterials, and pharmaceutical industry sectors.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Webber JL,Namivandi-Zangeneh R,Drozdek S,Wilk KA,Boyer C,Wong EHH,Bradshaw-Hajek BH,Krasowska M,Beattie DAdoi
10.1038/s41598-020-79702-3subject
Has Abstractpub_date
2021-01-18 00:00:00pages
1690issue
1issn
2045-2322pii
10.1038/s41598-020-79702-3journal_volume
11pub_type
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