Abstract:
:Raffinose family oligosaccharides (RFOs) are implicated in plant regulatory mechanisms of abiotic stresses tolerance and, despite their antinutritional proprieties in grain legumes, little information is available about the enzymes involved in RFO metabolism in Fabaceae species. In the present study, the systematic survey of legume proteins belonging to five key enzymes involved in the metabolism of RFOs (galactinol synthase, raffinose synthase, stachyose synthase, alpha-galactosidase, and beta-fructofuranosidase) identified 28 coding-genes in Arachis duranensis and 31 in A. ipaënsis. Their phylogenetic relationships, gene structures, protein domains, and chromosome distribution patterns were also determined. Based on the expression profiling of these genes under water deficit treatments, a galactinol synthase candidate gene (AdGolS3) was identified in A. duranensis. Transgenic Arabidopsis plants overexpressing AdGolS3 exhibited increased levels of raffinose and reduced stress symptoms under drought, osmotic, and salt stresses. Metabolite and expression profiling suggested that AdGolS3 overexpression was associated with fewer metabolic perturbations under drought stress, together with better protection against oxidative damage. Overall, this study enabled the identification of a promising GolS candidate gene for metabolic engineering of sugars to improve abiotic stress tolerance in crops, whilst also contributing to the understanding of RFO metabolism in legume species.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Vinson CC,Mota APZ,Porto BN,Oliveira TN,Sampaio I,Lacerda AL,Danchin EGJ,Guimaraes PM,Williams TCR,Brasileiro ACMdoi
10.1038/s41598-020-72191-4subject
Has Abstractpub_date
2020-09-17 00:00:00pages
15258issue
1issn
2045-2322pii
10.1038/s41598-020-72191-4journal_volume
10pub_type
杂志文章abstract::Encapsulation is essential to protect the air-sensitive components of organic light-emitting diodes (OLEDs) such as active layers and cathode electrodes. In this study, hybrid zirconium inorganic/organic nanolaminates were fabricated using remote plasma enhanced atomic layer deposition (PEALD) and molecular layer depo...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep40061
更新日期:2017-01-06 00:00:00
abstract::Salinity is a critical abiotic stress, which significantly impacts the agricultural yield worldwide. Identification of the molecular mechanisms underlying the salt tolerance in euhalophyte Suaeda salsa is conducive to the development of salt-resistant crops. In the present study, high-throughput RNA sequencing was per...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-61204-x
更新日期:2020-03-06 00:00:00
abstract::Sapphirinid copepods, which are marine zooplankton, exhibit tunable structural colors originating from a layered structure of guanine crystal plates. In the present study, the coloring portion of adult male of a sapphirinid copepod, Sapphirina nigromaculata, under the dorsal body surface was characterized to clarify t...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-59090-4
更新日期:2020-02-10 00:00:00
abstract::Identification of mutants with impairments in auxin biosynthesis and dynamics by forward genetic screening is hindered by the complexity, redundancy and necessity of the pathways involved. Furthermore, although a few auxin-deficient mutants have been recently identified by screening for altered responses to shade, eth...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep11923
更新日期:2015-07-06 00:00:00
abstract::Ballooning degeneration (BD) of hepatocytes is a distinguishing histological feature associated with the progression of nonalcoholic fatty liver disease (NAFLD). Under the assumption that NAFLD severity is associated with metabolic-stress we explored the hypothesis that heat shock 27 kDa protein 1 (HSP27), a protein c...
journal_title:Scientific reports
pub_type: 临床试验,杂志文章
doi:10.1038/srep22528
更新日期:2016-03-03 00:00:00
abstract::Glycans are biologically important structures synthesised by glycosyltransferase (GT) enzymes. Disruptive genetic null variants in GT genes can lead to serious illness but benign phenotypes are also seen, including antigenic differences on the red blood cell (RBC) surface, giving rise to blood groups. To characterise ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-24445-5
更新日期:2018-04-16 00:00:00
abstract::The concept of brain circuit disorders has been proposed for a variety of neuropsychiatric diseases, characterized by pathological disturbances of neuronal networks including changes in oscillatory signaling of re-entrant cortico-subcortical loops in the basal ganglia system. Parts of this circuitry play a pivotal rol...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-15872-x
更新日期:2017-11-14 00:00:00
abstract::High-throughput technology can now provide rich information on a person's biological makeup and environmental surroundings. Important discoveries have been made by relating these data to various health outcomes in fields such as genomics, proteomics, and medical imaging. However, cross-investigations between several h...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep36076
更新日期:2016-10-26 00:00:00
abstract::The fundamental mechanism how heterogeneous hepatic macrophage (Mφ) subsets fulfill diverse functions in health and disease has not been elucidated. We recently reported that CCR9+ inflammatory Mφs play a critical role in the course of acute liver injury. To clarify the origin and differentiation of CCR9+Mφs, we used ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep35146
更新日期:2016-10-11 00:00:00
abstract::Stromal capillary sheath cells in human spleens strongly express CD271, the low affinity nerve growth factor receptor p75. Serial sections of a representative adult human spleen were double-stained for CD271 versus smooth muscle alpha actin (SMA) plus CD34 to visualise capillary sheaths, the arterial tree and endothel...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-34105-3
更新日期:2018-10-24 00:00:00
abstract::Intergroup competition has an important impact on the survival and fitness of individuals in group-living species. However, factors influencing the probability of winning an encounter are not fully understood. We studied the influence of numerical advantage and location of the encounter on the chances of winning in ei...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep28457
更新日期:2016-06-22 00:00:00
abstract::In this study we analysed the acoustic properties and presence of haddock calls in the Arctic fjord Kongsfjorden (79° N-12° E, Svalbard Islands, Norway) in one year. Data were collected with three autonomous acoustic recorders located in the inner, middle, and outer parts of the fjord. The fjord is characterized by a ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-75415-9
更新日期:2020-10-26 00:00:00
abstract::Because of the unique selection rule, hyper-Raman scattering (HRS) can provide spectral information that linear Raman and infrared spectroscopy cannot obtain. However, the weak signal is the key bottleneck that restricts the application of HRS technique in study of the molecular structure, surface or interface behavio...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-30331-x
更新日期:2018-08-08 00:00:00
abstract::Chimpanzees (Pan troglodytes), one of humankinds' closest living relatives, are known to hunt and consume the meat of various animal taxa. Although some researchers have presented indirect evidence that chimpanzees may also prey on tortoises, until now, direct observations of this behaviour did not exist. Here, we pro...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-43301-8
更新日期:2019-05-23 00:00:00
abstract::The high failure strain of thermoplastic elastomers (TPEs) is a very desirable feature for rechargeable Li-ion batteries by improving the lifetime of high specific capacity anode materials that undergo mechanical fractures induced by large volume variations. In this work, poly(styrene-b-2-hydroxyethyl acrylate) called...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-40835-9
更新日期:2019-03-13 00:00:00
abstract::Triggering receptor expressed on myeloid cells-1(TREM-1) is a member of the superimmunoglobulin receptor family. We have previously shown that TREM-1 prolongs survival of macrophages treated with lipoolysaccharide through Egr2-Bcl2 signaling. Recent studies suggest a role for TREM-1 in viral immunity. Human immunodefi...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep42028
更新日期:2017-02-09 00:00:00
abstract::The ability of bacteria and fungi to communicate with each other is a remarkable aspect of the microbial world. It is recognized that volatile organic compounds (VOCs) act as communication signals, however the molecular responses by bacteria to fungal VOCs remain unknown. Here we perform transcriptomics and proteomics...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-00893-3
更新日期:2017-04-13 00:00:00
abstract::Poorer liver function is positively associated with diabetes in Mendelian randomization (MR) studies. Observationally, adiposity is associated with poorer liver function. To clarify the etiology, we assessed the association of liver enzymes with adiposity observationally and using two-sample MR for validation. In the ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-52489-8
更新日期:2019-11-14 00:00:00
abstract::Many insects possess the ability to detect fine fluctuations in the environmental CO2 concentration. In herbivorous species, plant-emitted CO2, in combination with other sensory cues, affect many behaviors including foraging and oviposition. In contrast to the comprehensive knowledge obtained on the insect olfactory p...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-76918-1
更新日期:2020-11-16 00:00:00
abstract::Type 2 diabetes mellitus (T2DM) can cause multidimensional cognitive deficits, among which working memory (WM) is usually involved at an early stage. However, the neural substrates underlying impaired WM in T2DM patients are still unclear. To clarify this issue, we utilized functional magnetic resonance imaging (fMRI)...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep23767
更新日期:2016-03-29 00:00:00
abstract::Nitrogen-doped graphene (N-graphene) has attractive properties that has been widely studied over the years. However, its possible formation process still remains unclear. Here, we propose a highly feasible formation mechanism of the graphitic-N doing in thermally treated graphene with ammonia by performing ab initio m...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep23495
更新日期:2016-03-22 00:00:00
abstract::In this work, lamellar MnFe2O4 was successfully planted on a wood surface through the association of hydrogen bonds via the one-pot hydrothermal method. Simultaneously, the fluoroalkylsilane (FAS-17) on the surface of the MnFe2O4 layer formed long-chain or network macromolecules through a poly-condensation process and...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep35549
更新日期:2016-10-17 00:00:00
abstract::Colorectal cancer (CRC) treatment is associated with a high morbidity which may result in a reduced health-related quality of life (HRQoL). The pre-operative measurement of handgrip strength (HGS) might be a tool to predict the patient's outcome after CRC surgery. The aim of this study was to evaluate the association ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-69806-1
更新日期:2020-08-03 00:00:00
abstract::Most Group I Clostridium botulinum strains harbor botulinum neurotoxin (bont) genes on their chromosome, while some carry these genes (including bont/a, bont/b, and bont/f) on large plasmids. Prior work in our laboratory demonstrated that Group I BoNT plasmids were mobilized to C. botulinum recipient strains containin...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-21342-9
更新日期:2018-02-15 00:00:00
abstract::Food constitutes a fuel of life for human beings. It is therefore of chief importance that their recognition system readily identifies the most relevant properties of food by drawing on semantic memory. One of the most relevant properties to be considered is the level of processing impressed by humans on food. We hypo...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-43919-8
更新日期:2019-05-15 00:00:00
abstract::This study aimed to explore the risk of dementia in a middle- and older-aged population with severe or profound hearing impairments. Data were collected for the Korean National Health Insurance Service-National Sample Cohort from 2002 to 2013. Participants aged 40 or older were selected. The 4,432 severely hearing-imp...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-33325-x
更新日期:2018-10-15 00:00:00
abstract::The present study aimed to explore a new source of montmorillonite and to develop an extraction and purification protocol for its isolation from raw clay samples acquired from the Koh-e-Suleiman mountain range in Pakistan. The process involved the collection of raw clay from the source, identification and quantificati...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-57511-y
更新日期:2020-01-17 00:00:00
abstract::Calcium-activated potassium ion channel-3.1 (KCa3.1) plays a pivotal role in the potassium-calcium exchange involved in atopy. This study aimed to explore the impact of lentiviral-mediated shRNA silencing KCa3.1 on allergic response in a murine allergic rhinitis (AR) model. The BALB/c mice were divided into four group...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep13127
更新日期:2015-08-14 00:00:00
abstract::We previously reported that chronic rhinosinusitis with nasal polyps (CRSwNP) was subdivided into four chronic rhinosinusitis (CRS) subtypes using the JESREC scoring system. We sought to identify the gene expression profile and biomarkers related with CRSwNP by RNA-sequence. RNA-sequencing was performed to identify di...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-29612-2
更新日期:2018-07-30 00:00:00
abstract::Dyschloremia is common in critically ill patients. However, little is known about the effects of dyschloremia on renal function in patients with acute kidney injury (AKI) requiring continuous renal replacement therapy (CRRT). A total of 483 patients who received CRRT for AKI were selected and divided into three groups...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-76798-5
更新日期:2020-11-12 00:00:00