Abstract:
OBJECTIVES:Escherichia coli and Enterobacter cloacae are two major pathogens causing urinary tract infection (UTI). Here we characterised the genome of an NDM-1-producing E. cloacae strain and an extended-spectrum β-lactamase (ESBL)-producing E. coli strain that were co-isolated from a UTI. METHODS:The genomes of E. cloacae strain EC32 and E. coli strain EC33 were sequenced using an Illumina HiSeqTM platform. The whole genome sequences were assembled using CLC Genomics Workbench and were annotated by the NCBI Prokaryotic Genome Annotation Pipeline (PGAP) server. Genomic analysis was further performed. RESULTS:The draft genome sequences of E. cloacae EC32 (ST66) and E. coli EC33 (ST1139) consisted of 89 contigs comprising 5 178 393 bp and 62 contigs comprising 5 057 666 bp, respectively. Including the blaNDM-1 gene, in total 13 resistance genes were identified in E. cloacae EC32 conferring resistance to β-lactams, rifampicin, phenicols, fosfomycin, macrolides, quinolones, sulfonamides and tetracycline. Nine resistance genes were identified in E. coli EC33 including the ESBL-encoding gene blaCTX-M-27. CONCLUSION:To our knowledge, this is the first report of genomic characterisation of NDM-1-producing E. cloacae and ESBL-producing E. coli co-isolated from a UTI in China. The two strains inhabiting in the same environment may allow the possibility of horizontal transfer of blaNDM-1 and blaCTX-M-27, making clinical treatment even difficult. This report also sheds light on geographically distinct Enterobacteriaceae strains from China enabling a comparative analysis of NDM-1- or ESBL-producing strains.
journal_name
J Glob Antimicrob Resistjournal_title
Journal of global antimicrobial resistanceauthors
Xu J,He Fdoi
10.1016/j.jgar.2019.04.007subject
Has Abstractpub_date
2019-06-01 00:00:00pages
198-200eissn
2213-7165issn
2213-7173pii
S2213-7165(19)30096-7journal_volume
17pub_type
杂志文章abstract:OBJECTIVES:Thousands of cases of multidrug-resistant tuberculosis (TB) have been observed in the Philippines, but studies on the Mycobacterium tuberculosis (MTB) genotypes that underlie the observed drug resistance profiles are lacking. This study aimed to analyse the whole genomes of clinical MTB isolates representing...
journal_title:Journal of global antimicrobial resistance
pub_type: 杂志文章
doi:10.1016/j.jgar.2018.08.009
更新日期:2018-12-01 00:00:00
abstract::Acinetobacter calcoaceticus-baumannii complex is a common cause of hospital-acquired infections (HAIs) globally, remarkable for its high rate of antibiotic resistance, including to carbapenems. There are few data on the resistance of A. baumannii in Vietnam, which are essential for developing evidence-based treatment ...
journal_title:Journal of global antimicrobial resistance
pub_type: 杂志文章
doi:10.1016/j.jgar.2014.05.003
更新日期:2014-12-01 00:00:00
abstract:OBJECTIVES:The aim of this study was to evaluate the efficacy of pyrimethamine-loaded poloxamer 407 nanomicelles on Plasmodium berghei strain NICD in vivo. METHODS:Pyrimethamine-loaded nanomicelles were prepared and their zeta potential, particle size and polydispersity index were measured. For antiplasmodial assessme...
journal_title:Journal of global antimicrobial resistance
pub_type: 杂志文章
doi:10.1016/j.jgar.2019.08.002
更新日期:2020-03-01 00:00:00
abstract:BACKGROUND:Inappropriate antimicrobial prescribing may harm patients and drive antimicrobial resistance. Junior doctors' knowledge of infectious diseases and antimicrobial prescribing is inadequate. Online spaced case-based learning can improve knowledge. OBJECTIVE:To develop infectious diseases and antimicrobial pres...
journal_title:Journal of global antimicrobial resistance
pub_type: 杂志文章
doi:10.1016/j.jgar.2020.02.008
更新日期:2020-09-01 00:00:00
abstract:OBJECTIVES:Mortality associated with carbapenemase-producing Enterobacteriaceae is high as there are few therapeutic options. Escherichia coli sequence type 410 (ST410) is currently an international high-risk clone and is responsible for a large number of clinical infections. Here we report the draft genome sequence of...
journal_title:Journal of global antimicrobial resistance
pub_type: 杂志文章
doi:10.1016/j.jgar.2019.10.027
更新日期:2019-12-01 00:00:00
abstract:BACKGROUND:OXA-48 is an Ambler class D β-lactamase that hydrolyses penicillin and imipenem but has poor hydrolytic activity against cephalosporins. However, very few clinical experiences of treating extended-spectrum β-lactamase (ESBL)-negative OXA-48 producers with cephalosporins have been published. OBJECTIVES:The a...
journal_title:Journal of global antimicrobial resistance
pub_type: 杂志文章
doi:10.1016/j.jgar.2019.02.016
更新日期:2019-12-01 00:00:00
abstract:OBJECTIVES:Corynebacterium urealyticum is a non-diphtherial urease-producing clinically relevant corynebacterium associated with urinary tract infections. Most clinical C. urealyticum isolates are multidrug-resistant. Whole-genome sequencing (WGS) of C. urealyticum VH4248 isolated from a clinical urine sample at Hospit...
journal_title:Journal of global antimicrobial resistance
pub_type: 杂志文章
doi:10.1016/j.jgar.2020.07.020
更新日期:2020-12-01 00:00:00
abstract:OBJECTIVES:Carbapenem-resistant Acinetobacter baumannii (CRAB) have emerged as a serious threat to public-health worldwide. This study aimed to determine the antimicrobial susceptibility of A. baumannii isolates in Iran and to investigate oxacillinase-encoding determinants and their association with insertion sequence ...
journal_title:Journal of global antimicrobial resistance
pub_type: 杂志文章
doi:10.1016/j.jgar.2018.12.011
更新日期:2019-09-01 00:00:00
abstract:OBJECTIVES:The aim of this study was to evaluate the role of residue 238 in CTX-M-15 and CTX-M-15G238C mutant with respect to carbapenems and various β-lactamase inhibitors. METHODS:A CTX-M-15G238C laboratory mutant was generated by site-directed mutagenesis from CTX-M-15 enzyme by replacing glycine 238 with cysteine....
journal_title:Journal of global antimicrobial resistance
pub_type: 杂志文章
doi:10.1016/j.jgar.2017.04.004
更新日期:2017-09-01 00:00:00
abstract:OBJECTIVE:Little is known about the treatment outcomes of HIV-negative drug-resistant tuberculosis (TB) patients in Malaysia. With respect to this issue, this study aimed to determine factors associated with unsuccessful treatment outcomes among drug-resistant TB patients at the Institute of Respiratory Medicine, Kuala...
journal_title:Journal of global antimicrobial resistance
pub_type: 杂志文章
doi:10.1016/j.jgar.2019.05.009
更新日期:2019-12-01 00:00:00
abstract:OBJECTIVES:The aim of this study was to determine the prevalence of nasal carriage of methicillin-resistant Staphylococcus aureus (MRSA) among healthcare workers (HCWs) compared with non-HCWs at Duhok city, Kurdistan Region, northern Iraq. METHODS:A total of 182 HCWs with different occupations and working in different...
journal_title:Journal of global antimicrobial resistance
pub_type: 杂志文章
doi:10.1016/j.jgar.2017.01.010
更新日期:2017-06-01 00:00:00
abstract:OBJECTIVES:This study was conducted to assess the prevalence of azole resistance in Aspergillus isolates from patients with haematological malignancies or who were undergoing haematopoietic stem cell transplantation and to identify the molecular mechanism of resistance. METHODS:In this 28-month prospective study invol...
journal_title:Journal of global antimicrobial resistance
pub_type: 杂志文章
doi:10.1016/j.jgar.2020.01.016
更新日期:2020-09-01 00:00:00
abstract::Antibiotic resistance genes are rapidly spread between pathogens and the normal flora, with plasmids playing an important role in their circulation. This study aimed to investigate antibiotic resistance plasmids in the gut microbiome of hospitalised patients. Stool samples were collected from seven inpatients at Sirir...
journal_title:Journal of global antimicrobial resistance
pub_type: 杂志文章
doi:10.1016/j.jgar.2016.03.001
更新日期:2016-09-01 00:00:00
abstract::Environmental fungicides are used in agriculture to reduce fungal spoilage of crops to a minimum, and the polyene macrolide natamycin is used as a food preservative. The use of natamycin in yoghurt has recently been authorised in the USA and some other countries. However, resistance development is a serious risk assoc...
journal_title:Journal of global antimicrobial resistance
pub_type: 杂志文章,评审
doi:10.1016/j.jgar.2017.10.024
更新日期:2018-06-01 00:00:00
abstract:OBJECTIVES:The aim of this study was to explore the genomic content of a blaKPC-2- and mcr-1-harbouring Escherichia coli strain and to clarify the molecular mechanism for the transmission of multidrug resistance. METHODS:Antimicrobial susceptibility testing was conducted by the broth microdilution method to determine ...
journal_title:Journal of global antimicrobial resistance
pub_type: 杂志文章
doi:10.1016/j.jgar.2020.08.023
更新日期:2020-12-01 00:00:00
abstract:BACKGROUND AND AIM:The global rise of antimicrobial resistance among bacterial strains is a rapidly growing challenge and is becoming a major public health concern. This study documents the worldwide spread and genotype distribution of human clinical isolates of New Delhi metallo-β-lactamase-producing Klebsiella pneumo...
journal_title:Journal of global antimicrobial resistance
pub_type: 杂志文章,评审
doi:10.1016/j.jgar.2020.10.016
更新日期:2020-12-01 00:00:00
abstract:OBJECTIVES:Here we report the finding of three copies of the blaKPC-2 gene on a plasmid in ST11 Klebsiella pneumoniae. METHODS:Carbapenem-resistant K. pneumoniae clinical strain WCHKP2 was subjected to whole-genome sequencing (WGS) using both a short-read Illumina HiSeq X10 platform and long-read MinION sequencer. Hyb...
journal_title:Journal of global antimicrobial resistance
pub_type: 杂志文章
doi:10.1016/j.jgar.2018.11.017
更新日期:2019-06-01 00:00:00
abstract:OBJECTIVES:This study aimed to characterise insertional mutations disturbing themgrB gene in carbapenem-resistant Klebsiella pneumoniae (CRKp). METHODS:A total of 118 clinical CRKp isolates were surveyed for polymyxin resistance and insertion sequence (IS) elements disruptingmgrB. RESULTS:Of the 118 isolates, 78 (66....
journal_title:Journal of global antimicrobial resistance
pub_type: 杂志文章
doi:10.1016/j.jgar.2020.11.003
更新日期:2020-11-24 00:00:00
abstract:OBJECTIVES:This meta-analysis was performed to compare polymyxin monotherapy and polymyxin-based combination therapy for carbapenem-resistant Klebsiella pneumoniae (CR-KP) infections. METHODS:We conducted searches on MEDLINE, Embase and Cochrane Collaborative database for both observational studies and randomised cont...
journal_title:Journal of global antimicrobial resistance
pub_type: 杂志文章
doi:10.1016/j.jgar.2020.08.024
更新日期:2020-12-01 00:00:00
abstract:OBJECTIVES:Staphylococcus cohnii has become increasingly recognized as a potential pathogen of clinically significant nosocomial and farm animal infections. This study was designed to determine the genome of a multidrug-resistant S. cohnii subsp. urealyticus strain SNUDS-2 isolated from a farmed duck in Korea. METHODS...
journal_title:Journal of global antimicrobial resistance
pub_type: 杂志文章
doi:10.1016/j.jgar.2017.05.008
更新日期:2017-09-01 00:00:00
abstract:OBJECTIVES:Here we describe a clinical isolate of Klebsiella pneumoniae ST11 harbouring both blaKPC-2 and rmtB genes in Japan. METHODS:A carbapenem- and aminoglycoside-resistant K. pneumoniae was isolated from an inpatient in Japan. Whole-genome sequencing (WGS) was performed using an Illumina next-generation sequence...
journal_title:Journal of global antimicrobial resistance
pub_type: 杂志文章
doi:10.1016/j.jgar.2018.11.026
更新日期:2019-06-01 00:00:00
abstract::Antibiotics are frequently used in food animal production in developing countries to promote the well-being and growth of animals. This practice provides some economic benefits to producers and consumers at large. Nevertheless, this practice is also associated with a number of concerns. A major concern has been that r...
journal_title:Journal of global antimicrobial resistance
pub_type: 杂志文章,评审
doi:10.1016/j.jgar.2019.07.031
更新日期:2020-03-01 00:00:00
abstract:OBJECTIVES:The aim of this study was to forecast the monthly incidence rates of infections [infections/1000 bed-days (IBD)] due to carbapenem-resistant Klebsiella pneumoniae (CRKP), carbapenem-resistant Pseudomonas aeruginosa (CRPA), carbapenem-resistant Acinetobacter baumannii (CRAB) and total carbapenem-resistant Gra...
journal_title:Journal of global antimicrobial resistance
pub_type: 杂志文章
doi:10.1016/j.jgar.2019.06.019
更新日期:2020-03-01 00:00:00
abstract:OBJECTIVES:The aim of this study was to define the relationship between age and response to tigecycline among patients treated for complicated skin and soft-tissue infections (cSSTIs) and complicated intra-abdominal infections (cIAIs). METHODS:Pooled data derived from five European observational studies on the use of ...
journal_title:Journal of global antimicrobial resistance
pub_type: 杂志文章
doi:10.1016/j.jgar.2019.03.018
更新日期:2019-09-01 00:00:00
abstract:OBJECTIVES:Farm animals have been recognised as important carriers and reservoirs of extended-spectrum β-lactamase (ESBL)-producing Escherichia coli. The aim of this study was to report the draft genome sequences of two multidrug-resistant (MDR) CTX-M-15-producing E. coli strains (47VL and 13B) isolated from different ...
journal_title:Journal of global antimicrobial resistance
pub_type: 杂志文章
doi:10.1016/j.jgar.2017.10.009
更新日期:2017-12-01 00:00:00
abstract:OBJECTIVES:The number of multidrug-resistant tuberculosis (MDR-TB) cases is rising worldwide. The present investigation aimed to evaluate, using the GenoType® MTBDRplus assay, the most common mutations associated with rifampicin (RIF) and isoniazid (INH) resistance among resistant strains in Morocco. METHODS:A total o...
journal_title:Journal of global antimicrobial resistance
pub_type: 杂志文章
doi:10.1016/j.jgar.2017.09.017
更新日期:2018-03-01 00:00:00
abstract:OBJECTIVES:Delafloxacin is a broad-spectrum anionic fluoroquinolone with activity against Gram-positive and Gram-negative organisms, including methicillin-resistant Staphylococcus aureus. Both oral and intravenous formulations were approved for use in the treatment of acute bacterial skin and skin-structure infections ...
journal_title:Journal of global antimicrobial resistance
pub_type: 杂志文章
doi:10.1016/j.jgar.2020.09.027
更新日期:2020-12-01 00:00:00
abstract:OBJECTIVES:Recently, a growing resistance to antimalarial drugs such as chloroquine, sulfadoxine-pyrimethamine, artemisinin derivatives and mefloquine has been observed. The pharmacokinetic limitation of the current therapy and multi-drug resistance has resulted in an urgent need to study the new antimalarial combinati...
journal_title:Journal of global antimicrobial resistance
pub_type: 杂志文章
doi:10.1016/j.jgar.2020.02.018
更新日期:2020-09-01 00:00:00
abstract::In the past few years, Europe has experienced an enormous influx of refugees and migrants owing to the ongoing civil war in Syria as well as conflicts, violence and instability in other Asian and African countries. Available data suggest that refugees carry a significant burden of multidrug-resistant (MDR) organisms, ...
journal_title:Journal of global antimicrobial resistance
pub_type: 杂志文章,评审
doi:10.1016/j.jgar.2017.03.013
更新日期:2017-09-01 00:00:00
abstract:OBJECTIVES:The rapid spread of multidrug-resistant (MDR) Acinetobacter baumannii poses a substantial threat for morbidity and mortality worldwide. In particular, carbapenem-resistant A. baumannii has caused a severe challenge to human health. Here we reported the draft genome sequence of A. baumannii S131434, an OXA-23...
journal_title:Journal of global antimicrobial resistance
pub_type: 杂志文章
doi:10.1016/j.jgar.2018.11.008
更新日期:2019-03-01 00:00:00