Abstract:
:: The highly conserved transcription factor Pax6 is involved in the development of the eyes, brain, and pancreas in vertebrates and invertebrates, whereas the additional expression pattern in other organs is still elusive. In this study, we cloned and characterized two pax6 homologs in blunt snout bream (Megalobrama amblycephala), named Mapax6a and Mapax6b. The protein alignment and phylogenetic tree showed that Mapax6a and Mapax6b were highly conserved compared with their counterparts in other species. Genomic information analysis revealed that the synteny conservation of Wilms tumor, Aniridia, genitourinary abnormalities, and mental retardation loci was also maintained in this species. By reverse transcription polymerase chain reaction, the expression of Mapax6a was later than that of Mapax6b which was found in the blastula stage, while the expression of Mapax6a started from the somite stage, and both of them persisted in a subsequent stage during the embryonic development. By RNA and protein detection, Mapax6a and Mapax6b were detected in the eye and brain as canonic patterns, and most importantly, they were also enriched in germ cells of the testis and ovary. Therefore, our findings validate the duplication of pax6 in fish, confirm the classical expression patterns in the brain and eye, and, for the first time, present a new acquisition of Mapax6a and Mapax6b in gonadal germ cells in particular. Therefore, our results enrich the expression pattern and evolutionary relationship of pax6 by suggesting that duplicated Mapax6 is involved in gametogenesis in Megalobrama amblycephala.
journal_name
Int J Mol Scijournal_title
International journal of molecular sciencesauthors
Pan Q,Xue T,Xia B,Luo J,Wang Q,Jiang Y,Yu M,Chen Tdoi
10.3390/ijms20071710subject
Has Abstractpub_date
2019-04-05 00:00:00issue
7issn
1422-0067pii
ijms20071710journal_volume
20pub_type
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journal_title:International journal of molecular sciences
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更新日期:2019-09-25 00:00:00
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更新日期:2019-01-21 00:00:00
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journal_title:International journal of molecular sciences
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doi:10.3390/ijms20246220
更新日期:2019-12-10 00:00:00
abstract:BACKGROUND:Ras homolog gene family member A (RhoA) is involved in Wnt-5a-induced migration of gastric and breast cancer cells. We investigated the roles of RhoA and Wnt-5a in ovarian carcinoma. METHODS:RhoA and Wnt-5a mRNA and protein expression in normal fallopian tube epithelium, benign tumors, primary ovarian carci...
journal_title:International journal of molecular sciences
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doi:10.3390/ijms141224187
更新日期:2013-12-12 00:00:00
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journal_title:International journal of molecular sciences
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doi:10.3390/ijms22031105
更新日期:2021-01-22 00:00:00
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journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms20204998
更新日期:2019-10-09 00:00:00
abstract::(1) Background: A high incidence of intervening sequence (IVS)4+919 G>A mutation with later-onset cardiac phenotype have been reported in a majority of Taiwan Fabry cohorts. Some evidence indicated that conventional biomarkers failed to predict the long-term progression and therapeutic outcome; (2) Methods: In this st...
journal_title:International journal of molecular sciences
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doi:10.3390/ijms19051480
更新日期:2018-05-16 00:00:00
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journal_title:International journal of molecular sciences
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更新日期:2016-05-11 00:00:00
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更新日期:2018-11-18 00:00:00
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journal_title:International journal of molecular sciences
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doi:10.3390/ijms161025657
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journal_title:International journal of molecular sciences
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doi:10.3390/ijms20020313
更新日期:2019-01-14 00:00:00
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更新日期:2020-07-08 00:00:00
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journal_title:International journal of molecular sciences
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doi:10.3390/ijms20112641
更新日期:2019-05-29 00:00:00
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journal_title:International journal of molecular sciences
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doi:10.3390/ijms12031605
更新日期:2011-01-01 00:00:00
abstract::Carbonic anhydrases (CAs) are a family of enzymes involved in the pH regulation of metabolically active cells/tissues. Upregulation of the CAIX/XII isoforms is associated with hypoxic tumours and clinically linked with malignant progression, treatment resistance and poor prognosis. The elucidation of the crystal struc...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms20236080
更新日期:2019-12-02 00:00:00
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journal_title:International journal of molecular sciences
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doi:10.3390/ijms131012169
更新日期:2012-09-25 00:00:00
abstract::Protein microarrays are powerful tools that are widely used in systems biology research. For infectious diseases, proteome microarrays assembled from proteins of pathogens will play an increasingly important role in discovery of diagnostic markers, vaccines, and therapeutics. Distinct formats of protein microarrays ha...
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pub_type: 杂志文章,评审
doi:10.3390/ijms11125165
更新日期:2010-01-01 00:00:00