Abstract：
:Protein microarrays are powerful tools that are widely used in systems biology research. For infectious diseases, proteome microarrays assembled from proteins of pathogens will play an increasingly important role in discovery of diagnostic markers, vaccines, and therapeutics. Distinct formats of protein microarrays have been developed for different applications, including abundance-based and function-based methods. Depending on the application, design issues should be considered, such as the need for multiplexing and label or label free detection methods. New developments, challenges, and future demands in infectious disease research will impact the application of protein microarrays for discovery and validation of biomarkers.
journal_name
Int J Mol Scijournal_title
International journal of molecular sciencesauthors
Natesan M,Ulrich RGdoi
10.3390/ijms11125165subject
Has Abstractpub_date
2010-01-01 00:00:00pages
5165-83issue
12issn
1422-0067pii
ijms-11-05165journal_volume
11pub_type
杂志文章,评审abstract：:For a long time, the tight junction (TJ) was known to form and regulate the paracellular barrier between epithelia and endothelial cell sheets. Starting shortly after the discovery of the proteins forming the TJ-mainly, the two families of claudins and TAMPs-several other functions have been discovered, a striking one...
journal_title：International journal of molecular sciences
pub_type： 社论
doi：10.3390/ijms21134612
更新日期：2020-06-29 00:00:00
abstract：:Barley (Hordeum vulgare L.) is an important cereal crop grown for both the feed and malting industries. The allelic dwarfing gene sdw1/denso has been used throughout the world to develop commercial barley varieties. Proteomic analysis offers a new approach to identify a broad spectrum of genes that are expressed in th...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms130810410
更新日期：2012-01-01 00:00:00
abstract：:Hederagenin (He) is a novel triterpene template for the development of new antitumor compounds. In this study, 26 new He⁻pyrazine derivatives were synthetized in an attempt to develop potent antitumor agents; they were screened for in vitro cytotoxicity against tumor and non-tumor cell lines. The majority of these der...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms19102994
更新日期：2018-09-30 00:00:00
abstract：:High-density lipoproteins (HDL) are known to have vasoprotective functions in peripheral arteries and many of these functions extend to brain-derived endothelial cells. Importantly, several novel brain-relevant HDL functions have been discovered using brain endothelial cells and in 3D bioengineered human arteries. The...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms20030462
更新日期：2019-01-22 00:00:00
abstract：:Although mitochondrial dysfunction has been implicated in aging, physical function decline, and several age-related diseases, an accessible and affordable measure of mitochondrial health is still lacking. In this study we identified the proteomic signature of muscular mitochondrial oxidative capacity in plasma. In 165...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21249540
更新日期：2020-12-15 00:00:00
abstract：:Evolution of reactive oxygen species (ROS), generated during the patho-physiological stress of nervous tissue, has been implicated in the etiology of several progressive human neurological disorders including Alzheimer's disease (AD) and amylotrophic lateral sclerosis (ALS). In this brief communication we used mixed i...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms13089615
更新日期：2012-01-01 00:00:00
abstract：:Type 2 Diabetes Mellitus (T2DM), one of the most common metabolic disorders, is caused by a combination of two primary factors: defective insulin secretion by pancreatic β-cells and the inability of insulin-sensitive tissues to respond appropriately to insulin. Because insulin release and activity are essential proces...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms21176275
更新日期：2020-08-30 00:00:00
abstract：:The effects of acidic polysaccharides purified from Gastrodia rhizome on blood pressure and serum lipid levels in spontaneously hypertensive rats (SHR) fed a high-fat diet were investigated. Acidic polysaccharides were purified from crude polysaccharides by DEAE-Sepharose CL-6B. Thirty-six male SHR were randomly divid...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms13010698
更新日期：2012-01-01 00:00:00
abstract：:Specific breast cancer (BC) subtypes are associated with bad prognoses due to the absence of successful treatment plans. The triple-negative breast cancer (TNBC) subtype, with estrogen (ER), progesterone (PR) and human epidermal growth factor-2 (HER2) negative receptor status, is a clinical challenge for oncologists, ...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21176337
更新日期：2020-09-01 00:00:00
abstract：:Maize (Zea mays L.) is a host to numerous pathogenic species that impose serious diseases to its ear and foliage, negatively affecting the yield and the quality of the maize crop. A considerable amount of research has been carried out to elucidate mechanisms of maize-pathogen interactions with a major goal to identify...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms161226106
更新日期：2015-11-30 00:00:00
abstract：:In eukaryotes, DNA is compacted into a complex structure known as chromatin. The unravelling of DNA is a crucial step in DNA repair, replication, transcription and recombination as this allows access to DNA for these processes. Failure to package DNA into the nucleosome, the individual unit of chromatin, can lead to g...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms12118063
更新日期：2011-01-01 00:00:00
abstract：:In vitro plant regeneration addresses basic questions of molecular reprogramming in the absence of embryonic positional cues. The process is highly dependent on the genotype and explant characteristics. However, the regulatory mechanisms operating during organ differentiation from in vitro cultures remain largely unkn...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms20092079
更新日期：2019-04-27 00:00:00
abstract：:Apolipoprotein M (apoM) is a plasma apolipoprotein that mainly associates with high-density lipoproteins. Hence, most studies on apoM so far have investigated its effect on and association with lipid metabolism and atherosclerosis. The insight into apoM biology recently took a major turn. ApoM was identified as a carr...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms14034419
更新日期：2013-02-25 00:00:00
abstract：:Eosinophilic colitis (EC) is a common cause of haematochezia in infants and young children. The exact pathomechanism is not understood, and the diagnosis is challenging. The role of microRNAs as key class of regulators of mRNA expression and translation in patients with EC has not been explored. Therefore, the aim of ...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms18051050
更新日期：2017-05-12 00:00:00
abstract：:Secondary caries often occurs at the tooth-composite margins. This study developed a novel bioactive composite containing DMAHDM (dimethylaminohexadecyl methacrylate) and NACP (nanoparticles of amorphous calcium phosphate), inhibiting caries at the enamel restoration margins in an in vitro saliva-derived biofilm secon...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21176369
更新日期：2020-09-02 00:00:00
abstract：:Acidovorax citrulli (A. citrulli) strains cause bacterial fruit blotch (BFB) in cucurbit crops and affect melon significantly. Numerous strains of the bacterium have been isolated from melon hosts globally. Strains that are aggressively virulent towards melon and diagnostic markers for detecting such strains are yet t...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms20112715
更新日期：2019-06-02 00:00:00
abstract：:Bradysia odoriphaga (Diptera: Sciaridae) is the most important pest of Chinese chive. Insecticides are used widely and frequently to control B. odoriphaga in China. However, the performance of the insecticides chlorpyrifos and clothianidin in controlling the Chinese chive maggot is quite different. Using next generati...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms18112445
更新日期：2017-11-17 00:00:00
abstract：:Desaturases are essentially required for unsaturated fatty acid (UFA) biosynthesis. We identified 10 genes encoding putative desaturases in the transcriptome database of the brown planthopper (BPH), Nilaparvata lugens. These include eight First Desaturase family genes, one cytochrome b5 fused desaturase gene (Nlug-Cyt...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms20061369
更新日期：2019-03-19 00:00:00
abstract：:Oxidative cellular damage caused by free radicals is known to contribute to the pathogenesis of various diseases such as cancer, diabetes, and neurodegenerative diseases, as well as to aging. The transcription factor nuclear factor erythroid 2-related factor 2 (Nrf2) and Kelch-like ECH-associated protein1 (Keap1) sign...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms19010026
更新日期：2017-12-22 00:00:00
abstract：:Acyl-CoA:glycerol-sn-3-phosphate acyltransferase (GPAT) is an enzyme responsible for the rate-limiting step in the synthesis of glycerophospholipids and triacylglycerol (TAG). The enzymes of mammalian species are classified into four isoforms; GPAT1 and GPAT2 are localized in the mitochondrial outer membrane, whereas ...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms20040964
更新日期：2019-02-22 00:00:00
abstract：:Natural killer (NK) cells play a significant and vital role in the first line of defense against infection through their ability to target cells without prior sensitization. They also contribute significantly to the activation and recruitment of both innate and adaptive immune cells through the production of a range o...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms21228864
更新日期：2020-11-23 00:00:00
abstract：:When cellular reducing enzymes fail to shield the cell from increased amounts of reactive oxygen species (ROS), oxidative stress arises. The redox state is misbalanced, DNA and proteins are damaged and cellular transcription networks are activated. This condition can lead to the initiation and/or to the progression of...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms131013104
更新日期：2012-10-12 00:00:00
abstract：:Carbonic anhydrase is a ubiquitous metalloenzyme that catalyzes the reversible interconversion of CO2/HCO3-. Equilibrium of these species is maintained by the action of carbonic anhydrase. Recent advances in magnetic resonance spectroscopy have allowed, for the first time, in vivo characterization of carbonic anhydras...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms21072442
更新日期：2020-04-01 00:00:00
abstract：:Acute or chronic administration of guanosine (GUO) induces anxiolytic-like effects, for which the adenosine (ADO) system involvement has been postulated yet without a direct experimental evidence. Thus, we aimed to investigate whether adenosine receptors (ARs) are involved in the GUO-mediated anxiolytic-like effect, e...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21239281
更新日期：2020-12-05 00:00:00
abstract：:The SGNH family esterases are highly effective biocatalysts due to their strong catalytic efficiencies, great stabilities, relatively small sizes, and ease of immobilization. Here, a novel SGNH family esterase (LaSGNH1) from Lactobacillus acidophilus NCFM, which has homologues in many Lactobacillus species, was identi...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21010091
更新日期：2019-12-21 00:00:00
abstract：:Chlorogenic acid (CGA) decreases colon cancer-cell proliferation but the combined anti-cancer effects of CGA with its major colonic microbial metabolites, caffeic acid (CA), 3-phenylpropionic acid (3-PPA) and benzoic acid (BA), needs elucidation as they occur together in colonic digesta. Caco-2 cancer cells were treat...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms19030723
更新日期：2018-03-03 00:00:00
abstract：:This study examined whether the conserved arginine cluster present within the 29-amino acid insert of the long form of the D₂ dopamine receptor (D2L-R) confers its predominant intracellular localization. We hypothesized that the conserved arginine cluster (RRR) located within the insert could act as an RXR-type endopl...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms17071152
更新日期：2016-07-19 00:00:00
abstract：:Hepatocyte growth factor (HGF) is an endogenously expressed bioactive substance that has a strong anti-apoptotic effect. In this study, we biochemically and histologically characterized the effects of rh-HGF on in vitro human hepatocyte injury and mouse acute liver failure (ALF) models, both of which were induced by a...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms20081821
更新日期：2019-04-12 00:00:00
abstract：:Obesity is a serious health challenge worldwide and is associated with various comorbidities, including dyslipidemia, type 2 diabetes, and cardiovascular disease. Developing effective strategies to prevent obesity is therefore of paramount importance. One potential strategy to reduce obesity is to consume calcium, whi...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms20123072
更新日期：2019-06-23 00:00:00
abstract：:Early diagnosis of cirrhosis and hepatocellular carcinoma (HCC) due to chronic Hepatitis C (CHC) remain clinical priorities. In this pilot study, we assessed serum microRNA (miRNA) expression to distinguish cirrhosis and HCC, alone and in combination with the aminotransferase-to-platelet ratio (APRI), Fibrosis 4 (FIB-...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms20040864
更新日期：2019-02-17 00:00:00
