Abstract:
:Specific breast cancer (BC) subtypes are associated with bad prognoses due to the absence of successful treatment plans. The triple-negative breast cancer (TNBC) subtype, with estrogen (ER), progesterone (PR) and human epidermal growth factor-2 (HER2) negative receptor status, is a clinical challenge for oncologists, because of its aggressiveness and the absence of effective therapies. In addition, proton therapy (PT) represents an effective treatment against both inaccessible area located or conventional radiotherapy (RT)-resistant cancers, becoming a promising therapeutic choice for TNBC. Our study aimed to analyze the in vivo molecular response to PT and its efficacy in a MDA-MB-231 TNBC xenograft model. TNBC xenograft models were irradiated with 2, 6 and 9 Gy of PT. Gene expression profile (GEP) analyses and immunohistochemical assay (IHC) were performed to highlight specific pathways and key molecules involved in cell response to the radiation. GEP analysis revealed in depth the molecular response to PT, showing a considerable immune response, cell cycle and stem cell process regulation. Only the dose of 9 Gy shifted the balance toward pro-death signaling as a dose escalation which can be easily performed using proton beams, which permit targeting tumors while avoiding damage to the surrounding healthy tissue.
journal_name
Int J Mol Scijournal_title
International journal of molecular sciencesauthors
Cammarata FP,Forte GI,Broggi G,Bravatà V,Minafra L,Pisciotta P,Calvaruso M,Tringali R,Tomasello B,Torrisi F,Petringa G,Cirrone GAP,Cuttone G,Acquaviva R,Caltabiano R,Russo Gdoi
10.3390/ijms21176337subject
Has Abstractpub_date
2020-09-01 00:00:00issue
17issn
1422-0067pii
ijms21176337journal_volume
21pub_type
杂志文章abstract::Streptococcus pneumoniae is a major meningitis-causing pathogen globally, bringing about significant morbidity and mortality, as well as long-term neurological sequelae in almost half of the survivors. Subsequent to nasopharyngeal colonisation and systemic invasion, translocation across the blood‒brain barrier (BBB) b...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms19113555
更新日期:2018-11-11 00:00:00
abstract::Photodynamic therapy is a procedure that can provide a selective eradication of neoplastic disease if sufficient drug, light, and oxygen are available. As this description suggests, it involves the photosensitization of malignant tissues to irradiation with photons in the visible range. While not suitable for tumors a...
journal_title:International journal of molecular sciences
pub_type: 历史文章,杂志文章
doi:10.3390/ijms160715188
更新日期:2015-07-03 00:00:00
abstract::The phytohormone auxin regulates various developmental programs in plants, including cell growth, cell division and cell differentiation. The auxin efflux carriers are essential for the auxin transport. To show an involvement of auxin transporters in the coordination of fruit development in bitter gourd, a juicy fruit...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms18112343
更新日期:2017-11-06 00:00:00
abstract::The bone marrow (BM) microenvironment plays an important role in supporting proliferation, survival and drug resistance of Multiple Myeloma (MM) cells. MM cells adhere to bone marrow stromal cells leading to the activation of tumour-promoting signaling pathways. Activation of the NFκB pathway, in particular, is centra...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms20030702
更新日期:2019-02-06 00:00:00
abstract::Non-alcoholic steatohepatitis (NASH) is characterized by the presence of steatosis, inflammation and hepatocyte injury and constitutes hepatic manifestation of the metabolic syndrome. The pathogenesis of NASH is complex and implicates cross-talk between different metabolically active sites, such as liver and adipose t...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms141019867
更新日期:2013-10-01 00:00:00
abstract::The adverse prognosis of most patients with ovarian cancer is related to recurrent disease caused by resistance to chemotherapeutic and targeted therapeutics. Besides their direct activity against tumor cells, monoclonal antibodies and tyrosine kinase inhibitors (TKIs) also influence the antitumoral activity of immune...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms20194693
更新日期:2019-09-22 00:00:00
abstract::A defining challenge of the 21st century is meeting the nutritional demands of the growing human population, under a scenario of limited land and water resources and under the specter of climate change. The Vavilov seed bank contains numerous landraces collected nearly a hundred years ago, and thus may contain 'geneti...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21113952
更新日期:2020-05-31 00:00:00
abstract::The mammalian target of rapamycin complex 1 (mTORC1) integrates amino acid (AA) availability to support protein synthesis and cell growth. Taste receptor type 1 member (T1R) is a G protein-coupled receptor that functions as a direct sensor of extracellular AA availability to regulate mTORC1 through Ca2+ stimulation an...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms17101684
更新日期:2016-10-11 00:00:00
abstract::Verticillium dahliae (V. dahliae) infects roots and colonizes the vascular vessels of host plants, significantly reducing the economic yield of cotton and other crops. In this study, the protein VdTHI20, which is involved in the thiamine biosynthesis pathway, was characterized by knocking out the corresponding VdTHI20...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21041378
更新日期:2020-02-18 00:00:00
abstract::Polyphosphoinositides (PPIns) are a family of seven lipid messengers that regulate a vast array of signalling pathways to control cell proliferation, migration, survival and differentiation. PPIns are differentially present in various sub-cellular compartments and, through the recruitment and regulation of specific pr...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms20122991
更新日期:2019-06-19 00:00:00
abstract::Chalcones are precursors of flavonoid biosynthesis in plants. Both flavonoids and chalcones are intensively investigated because of a large spectrum of their biological activities. Among others, anticancer and antiangiogenic effects account for the research interest of these substances. Because of an essential role in...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms19010027
更新日期:2017-12-22 00:00:00
abstract::Dry eye syndrome is the most common eye disease and it is caused by various reasons. As the balance of the tear film that protects the eyes is broken due to various causes, it becomes impossible to properly protect the eyes. In this study, the protective effects and underlying mechanisms of topical (E)-4-(2-(6-(2,6-di...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21103729
更新日期:2020-05-25 00:00:00
abstract::MicroRNAs (miRNAs) play an essential role in the regulation of almost all the biological processes, including melanogenesis. MiR-27a-3p is nearly six times higher in white alpaca skin compared to brown skin, which indicates that miR-27a-3p may be a candidate regulator for melanogenesis. Wnt3a plays an important role i...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms160510921
更新日期:2015-05-14 00:00:00
abstract::KMUP-1 (7-[2-[4-(2-chlorobenzene)piperazinyl]ethyl]-1,3-dimethylxanthine) has been reported to cause hepatic fat loss. However, the action mechanisms of KMUP-1 in obesity-induced steatohepatitis remains unclear. This study elucidated the steatohepatitis via matrix metallopeptidase 9 (MMP-9) and tumor necrosis factor α...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms17081345
更新日期:2016-08-17 00:00:00
abstract::Acute kidney injury (AKI) is a fatal medical episode caused by sudden kidney damage or failure, leading to the death of patients within a few hours or days. Previous studies demonstrated that exosomes derived from various mesenchymal stem/stromal cells (MSC-exosomes) have positive effects on renal injuries in multiple...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21134774
更新日期:2020-07-05 00:00:00
abstract::The rs10830963 variant of the Melatonin Receptor 1B (MTNR1B) gene is associated with the development of gestational diabetes mellitus (GDM). We hypothesized that carrying the rs10830963/G risk allele had effect on antenatal insulin therapy (AIT) initiation in GDM in a body mass index (BMI)-dependent manner. Design: In...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms19123734
更新日期:2018-11-23 00:00:00
abstract::An economical and environmentally friendly whey protein fractionation process was developed using supercritical carbon dioxide (sCO(2)) as an acid to produce enriched fractions of α-lactalbumin (α-LA) and β-lactoglobulin (β-LG) from a commercial whey protein isolate (WPI) containing 20% α-LA and 55% β-LG, through sele...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms13010240
更新日期:2012-01-01 00:00:00
abstract::Papillary thyroid cancer (PTC) is the most common tumor subtype of thyroid cancer. However, not all PTCs are responsive to current surgical and radioiodine treatment. The well-established clinical prognostic factors include tumor size, lymph node/distal metastasis, and extrathyroidal invasion. The RET/PTC-RAS-BRAF lin...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms18030636
更新日期:2017-03-15 00:00:00
abstract::Osteosarcoma therapy might be moving toward nanotechnology-based drug delivery systems to reduce the cytotoxicity of antineoplastic drugs and improve their pharmacokinetics. In this paper, we present, for the first time, an extensive chemical and in vitro characterization of dual-loaded photo- and chemo-active keratin...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms19113670
更新日期:2018-11-20 00:00:00
abstract::Peroxisome proliferator activated receptor α (PPARα) has the most relevant biological functions among PPARs. Activation by drugs and dietary components lead to major metabolic changes, from reduced triglyceridemia to improvement in the metabolic syndrome. Polymorphisms of PPARα are of interest in order to improve our ...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms20184378
更新日期:2019-09-06 00:00:00
abstract::Platelet P2Y12 is an important adenosine diphosphate (ADP) receptor that is involved in agonist-induced platelet aggregation and is a valuable target for the development of anti-platelet drugs. Here we characterise the effects of thio analogues of uridine triphosphate (UTP) on ADP-induced platelet aggregation. Using h...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms18020269
更新日期:2017-01-29 00:00:00
abstract::An increase in intracellular Ca2+ concentration ([Ca2+]i) plays a key role in controlling endothelial functions; however, it is still unclear whether endothelial Ca2+ handling is altered by type 2 diabetes mellitus, which results in severe endothelial dysfunction. Herein, we analyzed for the first time the Ca2+ respon...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21010250
更新日期:2019-12-30 00:00:00
abstract::Intraluminal vesicles (ILVs) are released into the extracellular space as exosomes after the fusion of multivesicular bodies (MVBs) with the plasma membrane. miRNAs are delivered to the raft-like region of MVB by RNA-binding proteins (RBPs). RNA loading into exosomes can be either through direct interaction between RN...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21228503
更新日期:2020-11-12 00:00:00
abstract::Today, cancer is understood as an epigenetic as well as genetic disease. The main epigenetic hallmarks of the cancer cell are DNA methylation and histone modifications. Proteins such as histone deacetylases (HDACs) that cause modifications of histones and other proteins can be targets for novel anticancer agents. Rece...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms160818252
更新日期:2015-08-06 00:00:00
abstract::Metastasis to the brain is a feared complication of systemic cancer, associated with significant morbidity and poor prognosis. A better understanding of the tumor metabolism might help us meet the challenges in controlling brain metastases. The study aims to characterize the metabolic profile of brain metastases of di...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms14012104
更新日期:2013-01-22 00:00:00
abstract::Oral biofilms attach onto both teeth surfaces and dental material surfaces in oral cavities. In the meantime, oral biofilms are not only the pathogenesis of dental caries and periodontitis, but also secondary caries and peri-implantitis, which would lead to the failure of clinical treatments. The material surfaces exp...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms19103157
更新日期:2018-10-14 00:00:00
abstract::Non-bullous congenital ichthyosiform erythroderma (NBCIE) is a hereditary disorder of keratinization caused by pathogenic variants in genes encoding enzymes important to lipid processing and terminal keratinocyte differentiation. Impaired function of these enzymes can cause pathologic epidermal scaling, significantly ...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms160921791
更新日期:2015-09-09 00:00:00
abstract::The melanin-inducing properties of cirsimaritin were investigated in murine B16F10 cells. Cirsimaritin is an active flavone with methoxy groups, which is isolated from the branches of Lithocarpus dealbatus. Tyrosinase activity and melanin content in murine B16F10 melanoma cells were increased by cirsimaritin in a dose...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms16048772
更新日期:2015-04-20 00:00:00
abstract:BACKGROUND:A growing body of data indicates that the physiology of the liver is sex-hormone dependent, with some types of liver failure occurring more frequently in males, and some in females. In males, in physiological conditions, testosterone acts via androgen receptors (AR) to increase insulin receptor (IR) expressi...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms22031242
更新日期:2021-01-27 00:00:00
abstract::Leukemia is the most common cancer in children, representing 30% of all childhood cancers. The disease arises from recurrent genetic insults that block differentiation of hematopoietic stem and/or progenitor cells (HSPCs) and drives uncontrolled proliferation and survival of the differentiation-blocked clone. Pediatri...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms17040461
更新日期:2016-03-29 00:00:00