Abstract:
:O-mannosylation is implicated in protein quality control in Saccharomyces cerevisiae due to the attachment of mannose to serine and threonine residues of un- or misfolded proteins in the endoplasmic reticulum (ER). This process also designated as unfolded protein O-mannosylation (UPOM) that ends futile folding cycles and saves cellular resources is mainly mediated by protein O-mannosyltransferases Pmt1 and Pmt2. Here we describe a genetic screen for factors that influence O-mannosylation in yeast, using slow-folding green fluorescent protein (GFP) as a reporter. Our screening identifies the RNA binding protein brefeldin A resistance factor 1 (Bfr1) that has not been linked to O-mannosylation and ER protein quality control before. We find that Bfr1 affects O-mannosylation through changes in Pmt1 and Pmt2 protein abundance but has no effect on PMT1 and PMT2 transcript levels, mRNA localization to the ER membrane or protein stability. Ribosome profiling reveals that Bfr1 is a crucial factor for Pmt1 and Pmt2 translation thereby affecting unfolded protein O-mannosylation. Our results uncover a new level of regulation of protein quality control in the secretory pathway.
journal_name
Int J Mol Scijournal_title
International journal of molecular sciencesauthors
Castells-Ballester J,Rinis N,Kotan I,Gal L,Bausewein D,Kats I,Zatorska E,Kramer G,Bukau B,Schuldiner M,Strahl Sdoi
10.3390/ijms20246220subject
Has Abstractpub_date
2019-12-10 00:00:00issue
24issn
1422-0067pii
ijms20246220journal_volume
20pub_type
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