Abstract：
:Pancreatic cancer remains a highly lethal malignancy. We have recently shown that simultaneous expression of Kras and mutant Tp53R175H promotes invasive ductal adenocarcinoma from pancreatic ductal cells. We hypothesized specific mutations in TP53 have divergent mechanisms of transforming ductal cells. In order to understand the role of mutant TP53 in transforming pancreatic ductal cells, we used a lentiviral system to express mutant TP53R175H and TP53R273H, two of the most frequently mutated TP53 alleles in pancreatic cancer patients, in immortalized, but not transformed, pancreatic ductal epithelial cells carrying a KRAS mutation (HPNE:KRASG12D). Mutant TP53 expression enhanced colony formation and an RPPA assay results revealed TP53R175H uniquely induced HSP70 expression in HPNE:KRASG12D cells. In the context of TP53R175H expression; we observed nuclear localization of HSP70. We performed immunoprecipitation experiments to show mutant p53R175H binds to HSP70. We also provide evidence mutant p53R175H is important for HSP70 stability and, more importantly, HSP70 is required for mutant p53 stability. These data are critical in the context of events leading to cellular transformation in the pancreas.
journal_name
Cancer Lettjournal_title
Cancer lettersauthors
Polireddy K,Singh K,Pruski M,Jones NC,Manisundaram NV,Ponnela P,Ouellette M,Van Buren G,Younes M,Bynon JS,Dar WA,Bailey JMdoi
10.1016/j.canlet.2019.03.047subject
Has Abstractpub_date
2019-07-01 00:00:00pages
122-130eissn
0304-3835issn
1872-7980pii
S0304-3835(19)30211-3journal_volume
453pub_type
杂志文章相关文献
CANCER LETTERS文献大全abstract：:The desensitization mechanism of the EGF receptor (EGFR) is important for the regulation of cancer cells. Although the phosphorylation of EGFR at Tyr1045 and Ser1046/1047 (Ser1046/7) reportedly accounts for such desensitization, the precise mechanism still remains unknown. Therefore, the present study investigated the...
journal_title：Cancer letters
pub_type： 杂志文章
doi：10.1016/j.canlet.2008.11.034
更新日期：2009-05-08 00:00:00
abstract：:The combined application of N-methyl-N-nitrosourea (MNU) and 7,12-dimethylbenz[alpha]anthracene (DMBA) was compared with the administration of each carcinogen alone as to the effectiveness of the induction of mammary carcinomas and the influence of H-ras oncogene activation in female Sprague-Dawley rats. At 50 days of...
journal_title：Cancer letters
pub_type： 杂志文章
doi：10.1016/s0304-3835(97)00293-0
更新日期：1997-11-25 00:00:00
abstract：:Loss of radioactivity from nude mice was determined after inoculation of human tumor cells prelabeled with [111In]indium oxine (111InOx). Elimination of 111In was increased somewhat by treating the mice with diphtheria toxin (DT), which is toxic selectively for human cells compared to mice. Calcium disodium edetate (C...
journal_title：Cancer letters
pub_type： 杂志文章
doi：10.1016/0304-3835(84)90134-4
更新日期：1984-09-01 00:00:00
abstract：:Microtubule targeting based therapies have revolutionized cancer treatment; however, resistance and side effects remain a major limitation. Therefore, novel strategies that can overcome these limitations are urgently needed. We made a novel discovery that fisetin, a hydroxyflavone, is a microtubule stabilizing agent. ...
journal_title：Cancer letters
pub_type： 杂志文章
doi：10.1016/j.canlet.2015.07.030
更新日期：2015-10-28 00:00:00
abstract：:Three series of monoacyl-2-O-beta-D-galactosylglycerols bearing an acyl chain of varying length, from C4 to C10, were studied due to their antitumor promoting effects on the activation of the Epstein-Barr virus early antigen (EBV-EA), such activation being induced by the tumor promoter 12-O-tetradecanoylphorbol-13-ace...
journal_title：Cancer letters
pub_type： 杂志文章
doi：10.1016/s0304-3835(99)00175-5
更新日期：1999-08-23 00:00:00
abstract：:Doxorubicin (dox) is an anthracycline antibiotic which is broadly used in solid tumors. Long-term therapy with this drug is accompanied by potentially lethal, dose-dependent side effects. Several reports suggest that oxygen free radicals produced during the metabolic activation of dox may have toxic effects on heart m...
journal_title：Cancer letters
pub_type： 杂志文章
doi：10.1016/0304-3835(95)04021-8
更新日期：1996-01-19 00:00:00
abstract：:Costunolide, the predominant sesquiterpene lactone in Saussureae radix, has been reported to exhibit potent chemopreventive effects on carcinogenesis. Effects of costunolide on cellular activation induced by a tumor-promoting phorbol ester 12-O-tetradecanoylphorbol-13-acetate (TPA) were investigated using a reporter g...
journal_title：Cancer letters
pub_type： 杂志文章
doi：10.1016/s0304-3835(00)00704-7
更新日期：2001-03-10 00:00:00
abstract：:6-(1-Hydroxyimino-4-methylpentyl)5,8-dimethyoxy 1,4-naphthoquinone S-52 (DMNQ S-52) was reported to have cytotoxic activity against L1210 leukemia cells. In the present study, we investigated the apoptotic mechanism of DMNQ S-52 in vitro and in vivo in murine solid cancer cells. DMNQ S-52 exerted cytotoxicity against ...
journal_title：Cancer letters
pub_type： 杂志文章
doi：10.1016/j.canlet.2005.02.042
更新日期：2006-02-20 00:00:00
abstract：:Given its contribution to malignant phenotypes of cancer, tumor hypoxia has been considered as a potential therapeutic problem. In the stressful microenvironment condition, hypoxia inducible factor 1 (HIF1) is well known to mediate the transcriptional adaptation of cells to hypoxia and acts as a central player for the...
journal_title：Cancer letters
pub_type： 杂志文章
doi：10.1016/j.canlet.2014.07.048
更新日期：2014-11-01 00:00:00
abstract：:Nuclear factor-kappa B (NF-kappaB) activation by tumor necrosis factor-alpha (TNF-alpha) attenuates the TNF-alpha-induced apoptosis pathway. Thus, blockage of NF-kappaB activity may improve the anti-cancer activity of TNF-alpha. HA14-1 induces apoptosis in various human cancer cells, and the molecular mechanisms of th...
journal_title：Cancer letters
pub_type： 杂志文章
doi：10.1016/j.canlet.2009.11.014
更新日期：2010-06-01 00:00:00
abstract：:Adult T-cell leukemia (ATL) is a fatal malignancy caused by infection with human T lymphotropic virus type-1 (HTLV-1). To search for a new biomarker of ATL, we analyzed sera from ATL patients using ProteinChip arrays. The spectral comparison of ATL patients with HTLV-1 carriers and healthy volunteers showed that the i...
journal_title：Cancer letters
pub_type： 杂志文章
doi：10.1016/j.canlet.2008.06.004
更新日期：2008-11-18 00:00:00
abstract：:The family of cyclin-dependent kinase complexes (Cdks) are well known for their role in the cell division cycle. What is less well known, however, is that Cdks also participate in a subset of apoptosis programs. Evidence for the role of Cdks in apoptosis comes from a variety of experimental approaches, including studi...
journal_title：Cancer letters
pub_type： 杂志文章,评审
doi：10.1016/j.canlet.2004.08.005
更新日期：2005-01-20 00:00:00
abstract：:Cancer prevention techniques include legislative regulation, chemoprevention, adherence to a "healthful" lifestyle, and specialized medical intervention. Attempts at cancer prevention have possibly avoided a dramatic increase in cancer incidence and mortality due to increasing dependence on the products of the chemica...
journal_title：Cancer letters
pub_type： 杂志文章,评审
doi：10.1016/0304-3835(90)90172-t
更新日期：1990-04-09 00:00:00
abstract：:The genomic aberration profile of chromosome 20q in distal CIN colon carcinomas was analysed using allelotyping and CGH arrays. Allelotyping revealed carcinomas with allelic imbalance along the full long arm, and carcinomas with fully non-aberrant 20q. Oligonucleotide-based CGH showed that among the carcinomas without...
journal_title：Cancer letters
pub_type： 杂志文章
doi：10.1016/j.canlet.2009.03.012
更新日期：2009-09-18 00:00:00
abstract：:The human multidrug resistance ABC transporters provide a protective function in our body against a large number of toxic compounds. These proteins, residing in the plasma membrane, perform an active, ATP-dependent extrusion of such xenobiotics. However, the same proteins are also used by the tumor cells to fight vari...
journal_title：Cancer letters
pub_type： 杂志文章,评审
doi：10.1016/j.canlet.2005.01.061
更新日期：2006-03-08 00:00:00
abstract：:Artemisinin, a compound isolated from the sweet wormwood Artemisia annua L., has previously been shown to have selective toxicity towards cancer cells in vitro. In the present experiment, we studied the potential of artemisinin to prevent breast cancer development in rats treated with a single oral dose (50mg/kg) of 7...
journal_title：Cancer letters
pub_type： 杂志文章
doi：10.1016/j.canlet.2005.01.019
更新日期：2006-01-08 00:00:00
abstract：:Epigenetic silencing of HOPX has been shown to be frequent and specific in human cancers. HOPX is thought as a tumor suppressor gene and its promoter methylation is the main mechanism of down-regulation. In non-hereditary breast cancer, since roles of epigenetic modifications are more critical than in other cancers, t...
journal_title：Cancer letters
pub_type： 杂志文章
doi：10.1016/j.canlet.2016.10.017
更新日期：2017-01-01 00:00:00
abstract：:Glioblastoma (GBM) has limited therapeutic options. DNA repair mechanisms contribute GBM cells to escape therapies and re-establish tumor growth. Multiple studies have shown that POLD2 plays a critical role in DNA replication, DNA repair and genomic stability. We demonstrate for the first time that POLD2 is highly exp...
journal_title：Cancer letters
pub_type： 杂志文章
doi：10.1016/j.canlet.2020.01.011
更新日期：2020-07-10 00:00:00
abstract：:Primary cultures of mouse embryo cells are more efficient in excising DNA-carcinogen adducts resulting from exposure to either 7-bromomethylbenz-[alpha]anthracene or the more carcinogen 7-bromomethyl-12-methylbenz[alpha]anthracene than are mouse L 929 cell suspension cultures. However, within each of these systems, th...
journal_title：Cancer letters
pub_type： 杂志文章
doi：10.1016/s0304-3835(79)80103-2
更新日期：1979-07-01 00:00:00
abstract：:Ovarian cancer represents the leading cause of death among patients with gynecological cancer. The genetic changes underlying the initiation and progression of ovarian cancer have not been well defined. However, non-random structural chromosomal changes have been identified with common chromosomal breakpoints. We have...
journal_title：Cancer letters
pub_type： 杂志文章
doi：10.1016/j.canlet.2005.04.010
更新日期：2006-04-08 00:00:00
abstract：:Cisplatin is an anticancer agent that is effective against several types of cancer, including gastric cancer. However, its therapeutic application is limited by its toxicity in normal tissues and complications caused in patients. In this study, we attempted to clarify how triptolide, an active component extracted from...
journal_title：Cancer letters
pub_type： 杂志文章
doi：10.1016/j.canlet.2012.01.006
更新日期：2012-06-28 00:00:00
abstract：:Despite the improved 5-year survival rate of breast cancer, triple-negative breast cancer (TNBC) remains a challenge due to lack of effective targeted therapy and higher recurrence and metastasis than other subtypes. To identify novel druggable targets and to understand its unique biology, we tried to implement 24 pat...
journal_title：Cancer letters
pub_type： 杂志文章
doi：10.1016/j.canlet.2018.04.020
更新日期：2018-08-01 00:00:00
abstract：:The malignant phenotype results from multiple genetic alterations, including the activation of oncogenes and inactivation of tumor suppressor genes. Activation of the Ki-ras oncogene has been implicated as an early event in the pathogenesis of lung adenocarcinomas in humans and experimental animal models. Previous stu...
journal_title：Cancer letters
pub_type： 杂志文章
doi：10.1016/s0304-3835(01)00647-4
更新日期：2001-10-22 00:00:00
abstract：:The capacity of enteric bacteria (E. coli, Salmonella, Pseudomonas, Shigella and Klebsiella) to catalyze the covalent binding of benzo(a)pyrene (BP), cholic acid, deoxycholic acid and cholesterol was investigated. In general, these bacteria were incapable of activating BP to a covalently bound product with calf thymus...
journal_title：Cancer letters
pub_type： 杂志文章
doi：10.1016/s0304-3835(77)94577-3
更新日期：1977-07-01 00:00:00
abstract：:Previous studies had established that transforming growth factor-beta1 (TGF-beta1) is highly expressed in bladder tumor, facilitating progression and spreading of the cancerous cells. Here, we report that both the number and the cytotoxic function of natural killer (NK) cells are decreased in patients with superficial...
journal_title：Cancer letters
pub_type： 杂志文章
doi：10.1016/j.canlet.2009.09.019
更新日期：2010-05-01 00:00:00
abstract：:We have previously shown that transforming growth factor (TGF)-beta up-regulates matrix metalloproteinase (MMP)-2 leading to the induction of oncogenic signaling in preneoplastic MCF10A human breast epithelial cells. The present study investigated the mechanism of transcriptional regulation of MMP-2 by TGF-beta in MCF...
journal_title：Cancer letters
pub_type： 杂志文章
doi：10.1016/j.canlet.2006.12.016
更新日期：2007-07-08 00:00:00
abstract：:A novel second generation retroviral producer cell strategy (an adenoviral/retroviral combined delivery system) has been developed by this laboratory. In the present studies, this delivery system was used to examine its delivery efficiency in vitro and in vivo by using a marker gene, LacZ, and a therapeutic gene, herp...
journal_title：Cancer letters
pub_type： 杂志文章
doi：10.1016/s0304-3835(02)00241-0
更新日期：2002-10-28 00:00:00
abstract：:Cancer is a multistep process that arises from a series of genetic and epigenetic events. With recent technological advances there has been a burst in genome sequencing and epigenetic studies revealing a plethora of alterations that may contribute to cancer. However, the great challenge for the cancer research communi...
journal_title：Cancer letters
pub_type： 杂志文章,评审
doi：10.1016/j.canlet.2016.03.029
更新日期：2017-02-28 00:00:00
abstract：:As a promising method for local tumor treatment, nanosecond pulsed electric field (nsPEF) ablation elicits a potent anti-tumor immune response. However, the mechanism of the nsPEF-mediated anti-tumor immune response and its effects on the tumor microenvironment remains unclear. Here, we demonstrated that nsPEF treatme...
journal_title：Cancer letters
pub_type： 杂志文章
doi：10.1016/j.canlet.2020.09.015
更新日期：2020-12-28 00:00:00
abstract：:The phenomenon of cellular dormancy has been observed in normal adult stem cells in many different tissues such as the skin, the intestine and the hematopoietic system. These dormant cells have been proposed to be important for life-long self-renewal and for the generation of the different cellular lineages. As tumor ...
journal_title：Cancer letters
pub_type： 杂志文章,评审
doi：10.1016/j.canlet.2013.04.019
更新日期：2013-11-28 00:00:00
